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You searched for: Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Subject term cell adhesion Remove constraint Subject term: cell adhesion
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1. 2-Arachidonoylglycerol modulates human endothelial cell/leukocyte interactions by controlling selectin expression through CB1 and CB2 receptors

Author:
Valeria Gasperi; Daniela Evangelista; Valerio Chiurchiù; Fulvio Florenzano; Isabella Savini; Sergio Oddi; Luciana Avigliano; Maria Valeria Catani; Mauro Maccarrone
Source:
international journal of biochemistry & cell biology 2014 v.51 pp. 79-88
ISSN:
1357-2725
Subject:
T-lymphocytes; cannabinoids; cell adhesion; cell movement; endothelium; glycoproteins; human umbilical vein endothelial cells; humans; plasma membrane; receptors; rolling; therapeutics; tumor necrosis factor-alpha
Abstract:
... Accumulated evidence points to a key role for endocannabinoids in cell migration, and here we sought to characterize the role of these substances in early events that modulate communication between endothelial cells and leukocytes. We found that 2-arachidonoylglycerol (2-AG) was able to initiate and complete the leukocyte adhesion cascade, by modulating the expression of selectins. A short exposur ...
DOI:
10.1016/j.biocel.2014.03.028

2. 3-D self-assembling leucine zipper hydrogel with tunable properties for tissue engineering

Author:
Chun-Chieh Huang; Sriram Ravindran; Ziying Yin; Anne George
Source:
Biomaterials 2014 v.35 no.20 pp. 5316-5326
ISSN:
0142-9612
Subject:
angiogenesis; animal models; cell adhesion; cellular microenvironment; humans; hydrocolloids; in vivo studies; leucine zipper; mice; polypeptides; stem cells; tissue engineering
Abstract:
... Peptide-based engineered hydrogel scaffolds present several advantages over traditional protein or polymeric hydrogels by imparting more robust control over hydrogel properties. In this manuscript, we report the synthesis and characterization of a leucine zipper (LZ) based self-assembling hydrogel for use in tissue engineering applications. Although, LZ hydrogels posses several advantages, the sta ...
DOI:
10.1016/j.biomaterials.2014.03.035
PubMed:
24713184
PubMed Central:
PMC4020426

3. 6-Methylsulfinylhexyl isothiocyanate modulates endothelial cell function and suppresses leukocyte adhesion

Author:
Takayuki Okamoto; Nobuyuki Akita; Masashi Nagai; Tatsuya Hayashi; Koji Suzuki
Source:
Natural medicines 2014 v.68 no.1 pp. 144-153
ISSN:
1340-3443
Subject:
adenosine; anti-inflammatory activity; vasculitis; necrosis; Eutrema japonica; platelet activation; chemokines; cell adhesion; human umbilical vein endothelial cells; macrophage activation; gene expression; homeostasis; blood coagulation; messenger RNA; humans; tumor necrosis factor-alpha; blood coagulation factors; anticoagulants; adhesion; cell proliferation; inflammation; interleukin-6; spices; Japan
Abstract:
... 6-Methylsulfinylhexyl isothiocyanate (6-MSITC) is an active compound in wasabi (Wasabia japonica Matsum.), which is one of the most popular spices in Japan. 6-MSITC suppresses lipopolysaccharide-induced macrophage activation, arachidonic- or adenosine diphosphate-induced platelet activation, and tumor cell proliferation. These data indicate that 6-MSITC has several biological activities involving ...
DOI:
10.1007/s11418-013-0784-x
PubMed:
23760613

4. A C-terminal fragment of fibulin-7 interacts with endothelial cells and inhibits their tube formation in culture

Author:
Susana de Vega; Nobuharu Suzuki; Risa Nonaka; Takako Sasaki; Patricia Forcinito; Eri Arikawa-Hirasawa; Yoshihiko Yamada
Source:
Archives of biochemistry and biophysics 2014 v.545 pp. 148-153
ISSN:
0003-9861
Subject:
actin; adhesion; angiogenesis; basement membrane; blood vessels; cell adhesion; human umbilical vein endothelial cells; in vitro studies; integrins; non-specific protein-tyrosine kinase; sprouting
Abstract:
... We have previously demonstrated that fibulin-7 (Fbln7) is expressed in teeth by pre-odontoblast and odontoblast cells, localized in the basement membrane and dentin matrices, and is an adhesion molecule for dental mesenchyme cells and odontoblasts. Fbln7 is also expressed in blood vessels by endothelial cells. In this report, we show that a recombinant C-terminal Fbln7 fragment (Fbln7-C) bound to ...
DOI:
10.1016/j.abb.2014.01.013
PubMed:
24480309
PubMed Central:
PMC3974681

5. A Comprehensive Phylogenetic and Structural Analysis of the Carcinoembryonic Antigen (CEA) Gene Family

Author:
Athanasia Pavlopoulou; Andreas Scorilas
Source:
Genome biology and evolution 2014 v.6 no.6 pp. 1314-1326
Subject:
amino acid composition; amino acid sequences; amino acids; angiogenesis; antigens; carcinogenesis; cell adhesion; chromosomes; genes; glycoproteins; homeostasis; humans; immunity; immunoglobulin A; insulin; mice; phylogeny; pregnancy; transcription (genetics)
Abstract:
... The carcinoembryonic antigen (CEA) gene family belongs to the immunoglobulin (Ig) superfamily and codes for a vast number of glycoproteins that differ greatly both in amino acid composition and function. The CEA family is divided into two groups, the carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) and the pregnancy-specific glycoproteins. The CEA family members are implicated in ...
DOI:
10.1093/gbe/evu103
PubMed:
24858421
PubMed Central:
PMC4079198

6. A Conserved Noncoding Sequence Can Function as a Spermatocyte-Specific Enhancer and a Bidirectional Promoter for a Ubiquitously Expressed Gene and a Testis-Specific Long Noncoding RNA

Author:
Misuzu Kurihara; Akira Shiraishi; Honoo Satake; Atsushi P. Kimura
Source:
Journal of Molecular Biology 2014 v.426 pp. 3069-3093
ISSN:
0022-2836
Subject:
acetylation; cell adhesion; chromatin; gene expression; green fluorescent protein; histones; mice; non-coding RNA; promoter regions; reporter genes; spermatocytes; testes; transcription (genetics)
Abstract:
... Tissue-specific gene expression is tightly regulated by various elements such as promoters, enhancers, and long noncoding RNAs (lncRNAs). In the present study, we identified a conserved noncoding sequence (CNS1) as a novel enhancer for the spermatocyte-specific mouse testicular cell adhesion molecule 1 (Tcam1) gene. CNS1 was located 3.4kb upstream of the Tcam1 gene and associated with histone H3K4 ...
DOI:
10.1016/j.jmb.2014.06.018

7. A PNIPAAm-based thermosensitive hydrogel containing SWCNTs for stem cell transplantation in myocardial repair

Author:
Xia Li; Jin Zhou; Zhiqiang Liu; Jun Chen; Shuanghong Lü; Hongyu Sun; Junjie Li; Qiuxia Lin; Boguang Yang; Cuimi Duan; Malcolm (Mengqiu) Xing; Changyong Wang
Source:
Biomaterials 2014 v.35 no.22 pp. 5679-5688
ISSN:
0142-9612
Subject:
adhesion; bioactive properties; carbon nanotubes; cell adhesion; cell transplantation; encapsulation; hydrogels; in vitro studies; infarction; myocardial infarction; myocardium; rats; stem cells; tissue engineering
Abstract:
... Poly (N-isopropylacrylamide) (PNIPAAm) hydrogel was a widely used carrier in therapeutic agent delivery. However, its bioactivities for encapsulated cells were not satisfactory. In the study, we aimed to determine whether modification with single-wall carbon nanotubes (SWCNTs) could improve the bioactivitis, especially supportive adhesion of PNIPAAm to encapsulated cells and favor their efficacy i ...
DOI:
10.1016/j.biomaterials.2014.03.067

8. A bioactive elastin-like recombinamer reduces unspecific protein adsorption and enhances cell response on titanium surfaces

Author:
Emiliano Salvagni; Geoffrey Berguig; Elisabeth Engel; J. Carlos Rodriguez-Cabello; Geraldine Coullerez; Marcus Textor; Josep A. Planell; F. Javier Gil; Conrado Aparicio
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.114 pp. 225-233
ISSN:
0927-7765
Subject:
X-ray photoelectron spectroscopy; absorption; adsorption; bioactive properties; blood proteins; blood serum; cell adhesion; chemical bonding; coatings; colloids; mechanical stress; titanium
Abstract:
... We present the immobilization on synthetic substrates of elastin-like recombinamers (ELR) that combine a bioactive motif for cell adhesion with protein antifouling properties. Physical adsorption of the recombinamers and covalent-grafting through organosilane chemistry were investigated. The biochemically-modified surfaces were thoroughly characterized and tested for protein absorption in serum by ...
DOI:
10.1016/j.colsurfb.2013.10.008

9. A combinatorial study of nanocomposite hydrogels: on-chip mechanical/viscoelastic and pre-osteoblast interaction characterization

Author:
M. B. Oliveira; G. M. Luz; J. F. Mano
Source:
Journal of materials chemistry B 2014 v.2 no.34 pp. 5627-5638
ISSN:
2050-7518
Subject:
bioactive properties; biocompatible materials; cell adhesion; cell culture; cell proliferation; cell structures; chitosan; collagen; fibroblasts; granulation tissue; hydrogels; hydrophobicity; image analysis; mechanical properties; modulus of elasticity; nanocomposites; nanoparticles; regression analysis; tissue engineering; viscoelasticity
Abstract:
... Nanocomposite hydrogels were prepared in a combinatorial way with chitosan, bioglass nanoparticles (BG-NPs) and distinct amounts of crosslinker (genipin), in a total of 30 formulations. Such miniaturized hydrogels were prepared by dispensing the precursor solutions in wettable spots previously patterned onto superhydrophobic surfaces. The chips were used as platforms to analyze the biomaterials on ...
DOI:
10.1039/c4tb00437j

10. A composite demineralized bone matrix – Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow

Author:
Tianyong Hou; Zhiqiang Li; Fei Luo; Zhao Xie; Xuehui Wu; Junchao Xing; Shiwu Dong; Jianzhong Xu
Source:
Biomaterials 2014 v.35 no.22 pp. 5689-5699
ISSN:
0142-9612
Subject:
biocompatibility; biocompatible materials; bone marrow; cell adhesion; cellular microenvironment; comorbidity; ethics; porosity; stem cells
Abstract:
... The need for suitable bone grafts is high; however, there are limitations to all current graft sources, such as limited availability, the invasive harvest procedure, insufficient osteoinductive properties, poor biocompatibility, ethical problems, and degradation properties. The lack of osteoinductive properties is a common problem. As an allogenic bone graft, demineralized bone matrix (DBM) can ov ...
DOI:
10.1016/j.biomaterials.2014.03.079

11. A correlation study of protein adsorption and cell behaviors on substrates with different densities of PEG chains

Author:
Mingcong Sun; Jun Deng; Zengchao Tang; Jindan Wu; Dan Li; Hong Chen; Changyou Gao
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.122 pp. 134-142
ISSN:
0927-7765
Subject:
adhesion; adsorption; blood serum; bovine serum albumin; cell adhesion; colloids; fibronectins; fluorescence microscopy; in vitro culture; mushrooms; myocytes; polyethylene glycol; radiolabeling; smooth muscle
Abstract:
... The adsorption of proteins, in particular fibronectin (Fn), was studied on poly(ethylene glycol) (PEG, 5kDa)-grafted surfaces, and was correlated with the adhesion behaviors of smooth muscle cells (SMCs). The PEG molecules were covalently grafted on aldehyde-activated substrates with different densities of amino groups. The thickness of PEG layer increased nearly 10 fold in a hydrated state, reach ...
DOI:
10.1016/j.colsurfb.2014.06.041

12. A facile preparation of poly(ethylene oxide)-modified medical polyurethane to improve hemocompatibility

Author:
Xuefeng Zhou; Tianzhu Zhang; Dawei Guo; Ning Gu
Source:
Colloids and Surfaces A: Physicochemical and Engineering Aspects 2014 v.441 pp. 34-42
ISSN:
0927-7757
Subject:
adhesion; atomic force microscopy; biocompatibility; biocompatible materials; blood platelets; catalysts; cell adhesion; coatings; deformation; ethylene oxide; hydrophilicity; infrared spectroscopy; molecular weight; polyethylene glycol; polyurethanes; reflectance; scanning electron microscopy
Abstract:
... Poly(ethylene oxide) (PEO) has been widely applied in the modification of biomaterials because it shows high hydrophilicity and excellent biocompatibility. In the current study, the surface of medical polyurethane (PU) films was modified to improve hydrophilicity and hemocompatibility by a facile method without catalyst. PU films were modified directly using poly(ethylene oxide) (PEO) with various ...
DOI:
10.1016/j.colsurfa.2013.08.070

13. A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces

Author:
Corinne A. Hoesli; Alain Garnier; Pierre-Marc Juneau; Pascale Chevallier; Carl Duchesne; Gaétan Laroche
Source:
Biomaterials 2014 v.35 no.3 pp. 879-890
ISSN:
0142-9612
Subject:
biocompatible materials; cell adhesion; cell proliferation; endothelial cells; focal adhesions; image analysis; integrins; non-specific protein-tyrosine kinase; phosphorylation; prostheses
Abstract:
... The long-term patency rates of vascular grafts and stents are limited by the lack of surface endothelialisation of the implanted materials. We have previously reported that GRGDS and WQPPRARI peptide micropatterns increase the endothelialisation of prosthetic materials in vitro. To investigate the mechanisms by which the peptide micropatterns affect endothelial cell adhesion and proliferation, a T ...
DOI:
10.1016/j.biomaterials.2013.09.076

14. A peptide functionalized poly(ethylene glycol) (PEG) hydrogel for investigating the influence of biochemical and biophysical matrix properties on tumor cell migration

Author:
Samir P. Singh; Michael P. Schwartz; Justin Y. Lee; Benjamin D. Fairbanks; Kristi S. Anseth
Source:
Biomaterials science 2014 v.2 no.7 pp. 1024-1034
ISSN:
2047-4849
Subject:
adhesion; cell adhesion; cell culture; cell movement; crosslinking; fibrosarcoma; human cell lines; humans; hydrogels; ligands; models; neoplasm cells; peptides; photochemistry; polyethylene glycol
Abstract:
... To address the challenges associated with defined control over matrix properties in 3D cell culture systems, we employed a peptide functionalized poly(ethylene glycol) (PEG) hydrogel matrix in which mechanical modulus and adhesive properties were tuned. An HT-1080 human fibrosarcoma cell line was chosen as a model for probing matrix influences on tumor cell migration using the PEG hydrogel platfor ...
DOI:
10.1039/c4bm00022f
PubMed:
25105013
PubMed Central:
PMC4120072

15. A proteomic approach for the elucidation of the specificity of ectodomain shedding

Author:
Kyoko Shirakabe; Yoshio Shibagaki; Akihiko Yoshimura; Shigeo Koyasu; Seisuke Hattori
Source:
Journal of proteomics 2014 v.98 pp. 233-243
ISSN:
1874-3919
Subject:
cell adhesion; cell growth; cell movement; immune response; integrins; lipopolysaccharides; macrophages; mice; pathogenesis; post-translational modification; proteomics; screening
Abstract:
... Ectodomain shedding (shedding) is a posttranslational modification mechanism, which liberates extracellular domains of membrane proteins through juxtamembrane processing. Because shedding alters cell characteristics in a rapid and irreversible manner, it must be strictly regulated. However, the regulatory mechanisms of shedding in response to environmental changes remain obscure. To evaluate the r ...
DOI:
10.1016/j.jprot.2014.01.012

16. A silica-gold core-shell structure to mimic the large size of gold particles for promoting cell growth: A comparative study of the silica core size and the nanogold amount in the shell

Author:
Xiaoqin Li; Jinru Li; Long Jiang
Source:
Colloids and Surfaces A: Physicochemical and Engineering Aspects 2014 v.459 pp. 211-216
ISSN:
0927-7757
Subject:
biosafety; cell adhesion; cell growth; cell proliferation; cell viability; coculture; cytotoxicity; energy-dispersive X-ray analysis; gold; nanogold; scanning electron microscopes; scanning electron microscopy; seeds; silica; tissue engineering
Abstract:
... Recently, the biosafety of gold nanomaterials has attracted more attention. Some consider them as an adverse factor for cell growth, whereas others regard them as biocompatible. In this work, we reported a type of silica-gold core-shell structure (SiO2@AuNPs) with the diameter more than 70nm that could promote cell growth. The synthesis of SiO2@AuNPs adopted a step-by-step protocol involving the f ...
DOI:
10.1016/j.colsurfa.2014.07.013

17. A spatial patternable macroporous hydrogel with cell-affinity domains to enhance cell spreading and differentiation

Author:
Jing Sun; Dan Wei; Yuda Zhu; Meiling Zhong; Yicong Zuo; Hongsong Fan; Xingdong Zhang
Source:
Biomaterials 2014 v.35 no.17 pp. 4759-4768
ISSN:
0142-9612
Subject:
alginates; bioengineering; bone formation; cell adhesion; cell differentiation; cytoskeleton; gelatin; hybridization; hydrogels; macropores; microparticles; models; porous media; tissues
Abstract:
... Cell adhesion and spreading are two essential factors for anchorage-dependent cells such as osteocytes. An adhesive macroporous hydrogel system, in which cell-affinitive domains and sufficient cytoskeleton reorganization space were simultaneously constructed, was proposed in this report to support cell adhesion and spreading, respectively, and facilitate cell differentiation and function establish ...
DOI:
10.1016/j.biomaterials.2014.02.041

18. A stimulatory effect of Ca₃ZrSi₂O₉ bioceramics on cementogenic/osteogenic differentiation of periodontal ligament cells

Author:
Xufang Zhang; Pingping Han; Anjali Jaiprakash; Chengtie Wu; Yin Xiao
Source:
Journal of materials chemistry B 2014 v.2 no.10 pp. 1415-1423
ISSN:
2050-7518
Subject:
alkaline phosphatase; beta catenin; biocompatible materials; bone formation; calcium; calcium silicate; cell adhesion; cell growth; cell proliferation; ceramics; chemistry; gene expression; gene expression regulation; genes; humans; ligaments; osteoblasts; periodontitis; phosphates; powders; signal transduction; silicon; tissue engineering; tissue repair; zirconium
Abstract:
... The regeneration of periodontal tissues to cure periodontitis remains a medical challenge. Therefore, it is of great importance to develop a novel biomaterial that could induce cementogenesis and osteogenesis in periodontal tissue engineering. Calcium silicate (Ca–Si) based ceramics have been found to be potential bioactive materials due to their osteostimulatory effect. Recently, it is reported t ...
DOI:
10.1039/c3tb21663b

19. A tough, precision-porous hydrogel scaffold: Ophthalmologic applications

Author:
Wenqi Teng; Thomas J. Long; Qianru Zhang; Ke Yao; Tueng T. Shen; Buddy D. Ratner
Source:
Biomaterials 2014 v.35 no.32 pp. 8916-8926
ISSN:
0142-9612
Subject:
acrylamides; cell adhesion; hydrogels; in vitro studies; mechanical properties; polyacrylic acid; polymerization; porosity; rabbits; tissue engineering; tissues
Abstract:
... Appropriate mechanical properties and highly interconnected porosity are important properties for tissue engineering scaffolds. However, most existing hydrogel scaffolds suffer from poor mechanical properties limiting their application. Furthermore, it is relatively infrequent that precision control is achieved over pore size and structure of the scaffold because there are relatively few current t ...
DOI:
10.1016/j.biomaterials.2014.07.013

20. A transgenic insertion on mouse chromosome 17 inactivates a novel immunoglobulin superfamily gene potentially involved in sperm–egg fusion

Author:
Diego Lorenzetti; Christophe Poirier; Ming Zhao; Paul A. Overbeek; Wilbur Harrison; Colin E. Bishop
Source:
Mammalian genome 2014 v.25 no.3-4 pp. 141-148
ISSN:
0938-8990
Subject:
cell adhesion; chromosomes; cytoplasm; diploidy; egg membranes; eggs; genes; genetically modified organisms; haploidy; immunoglobulins; in vivo studies; membrane proteins; mice; mutants; mutation; plasma membrane; spermatozoa; testes; zygote
Abstract:
... Fertilization is the process that leads to the formation of a diploid zygote from two haploid gametes. This is achieved through a complex series of cell-to-cell interactions between a sperm and an egg. The final event of fertilization is the fusion of the gametes’ membranes, which allows the delivery of the sperm genetic material into the egg cytoplasm. In vivo studies in the laboratory mouse have ...
DOI:
10.1007/s00335-013-9491-x
PubMed:
24275887

21. Accelerated bone growth in vitro by the conjugation of BMP2 peptide with hydroxyapatite on titanium alloy

Author:
Yanli Cai; Xiaoyan Wang; Chye Khoon Poh; Hark Chuan Tan; Min Tun Soe; Sam Zhang; Wilson Wang
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.116 pp. 681-686
ISSN:
0927-7765
Subject:
X-ray diffraction; X-ray photoelectron spectroscopy; alkaline phosphatase; alloys; bioactive properties; calcium; cell adhesion; cell culture; coatings; colloids; fluorescence emission spectroscopy; fluorescence microscopy; gene expression; hydroxyapatite; in vitro studies; ions; medical equipment; orthopedics; sodium chloride; titanium
Abstract:
... Titanium alloys have been widely used in orthopedic practice due to their inherent bioactivity, however it is still insufficient to truly and reliably incorporate into living bone. In this work, polydopamine film was employed to induce the growth of hydroxyapatite (HA) on titanium alloy to enhance its osteoconductivity. Bone morphogenetic protein-2 (BMP2) peptide was absorbed into the HA particles ...
DOI:
10.1016/j.colsurfb.2013.11.004

22. Acetyltransferase SAS2 and sirtuin SIR2, respectively, control flocculation and biofilm formation in wine yeast

Author:
María E. Rodriguez; Helena Orozco; Jesús M. Cantoral; Emilia Matallana; Agustín Aranda
Source:
FEMS yeast research 2014 v.14 no.6 pp. 845-857
ISSN:
1567-1356
Subject:
Saccharomyces cerevisiae; acetylation; adhesion; agar; biofilm; cell adhesion; encapsulation; fermentation; flocculation; genetic engineering; grape juice; histone deacetylase; maltose; phenotype; promoter regions; regulatory proteins; transcription (genetics); wine yeasts; wines
Abstract:
... Cell‐to‐cell and cell‐to‐environment interactions of microorganisms are of substantial relevance for their biotechnological use. In the yeast Saccharomyces cerevisiae, flocculation can be an advantage to clarify final liquid products after fermentation, and biofilm formation may be relevant for the encapsulation of strains of interest. The adhesion properties of wine yeast strains can be modified ...
DOI:
10.1111/1567-1364.12173

23. Actin filament dynamics and endothelial cell junctions: the Ying and Yang between stabilization and motion

Author:
Hans Schnittler; Muna Taha; Maria Odenthal Schnittler; Abdallah Abu Taha; Nico Lindemann; Jochen Seebach
Source:
Cell and tissue research 2014 v.355 no.3 pp. 529-543
ISSN:
0302-766X
Subject:
actin; adhesion; blood; cell adhesion; endothelial cells; endothelium; exocytosis; intercellular junctions; microfilaments; pseudopodia; solutes
Abstract:
... The vascular endothelium is a cellular interface between the blood and the interstitial space of tissue, which controls the exchange of fluid, solutes and cells by both transcellular and paracellular means. To accomplish the demands on barrier function, the regulation of the endothelium requires quick and adaptive mechanisms. This is, among others, accomplished by actin dynamics that interdependen ...
DOI:
10.1007/s00441-014-1856-2
PubMed:
24643678

24. Addition of 1,3-β-d-glucan to chitosan-based composites enhances osteoblast adhesion, growth, and proliferation

Author:
Agata Przekora; Grazyna Ginalska
Source:
International journal of biological macromolecules 2014 v.70 pp. 474-481
ISSN:
0141-8130
Subject:
adhesion; biocompatible materials; biocomposites; bones; cell adhesion; cell proliferation; chitosan; cytotoxicity; fluorescence; glucans; osteoblasts; staining; tissue engineering
Abstract:
... The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-d-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells. Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining. Cell adhesion was determined qua ...
DOI:
10.1016/j.ijbiomac.2014.07.035

25. Adhesion and Proliferation of Human Mesenchymal Stem Cells from Dental Pulp on Porous Silicon Scaffolds

Author:
Collart-Dutilleul Pierre-Yves; Secret Emilie; Panayotov Ivan; Deville de Périère Dominique; Martín-Palma Raúl J.; Torres-Costa Vicente; Martin Marta; Gergely Csilla; Durand Jean-Olivier; Cunin Frédérique; Cuisinier Frédéric J.
Source:
ACS Applied Materials & Interfaces 2014 v.6 no.3 pp. 1719-1728
ISSN:
1944-8252
Subject:
adhesion; cell adhesion; cell proliferation; chemical treatment; culture media; enzyme activity; fluorescence microscopy; growth factors; humans; medicine; mitosis; oxidation; physicochemical properties; porosity; scanning electron microscopy; silicon; stem cells; therapeutics; tissue engineering; tooth pulp
Abstract:
... In regenerative medicine, stem-cell-based therapy often requires a scaffold to deliver cells and/or growth factors to the injured site. Porous silicon (pSi) is a promising biomaterial for tissue engineering as it is both nontoxic and bioresorbable. Moreover, surface modification can offer control over the degradation rate of pSi and can also promote cell adhesion. Dental pulp stem cells (DPSC) are ...
DOI:
10.1021/am4046316

26. Adhesion and differentiation of human mesenchymal stem cells on plasma-functionalized graphenes with different feeding gases

Author:
Ku Youn Baik; Jinsung Choi; Hyeseon Gwon; Jaewon Cho; Yun Ki Kim; Pankaj Attri; Un Jeong Kim; Ranju Jung
Source:
Carbon 2014 v.77 pp. 302-310
ISSN:
0008-6223
Subject:
adhesion; adsorption; bone formation; cell adhesion; cell differentiation; cell engineering; cell proliferation; fibronectins; gases; graphene; humans; hydrogen; myoblasts; nitrogen; osteoblasts; oxygen
Abstract:
... Surface functionalisation of graphene layer has been investigated to improve the efficiencies in bio-applications such as stem cell differentiation or protein sensing. Plasma treatment can induce various functionalities on graphene surface simply by changing feeding gas. In this study, the surface chemistry of graphene is modified by plasma treatment using three different feeding gases of H2, N2, ...
DOI:
10.1016/j.carbon.2014.05.033

27. Adhesion and growth of HUVEC endothelial cells on films of enzymatically functionalized chitosan with phenolic compounds

Author:
Abdulhadi Aljawish; Lionel Muniglia; Jordane Jasniewski; Amira Klouj; Joël Scher; Isabelle Chevalot
Source:
Process Biochemistry 2014 v.49 pp. 863-871
ISSN:
1359-5113
Subject:
adhesion; adsorption; biocompatible materials; cell adhesion; cell viability; chitosan; ferulic acid; human umbilical vein endothelial cells; phenols; phenotype
Abstract:
... Human Umbilical Vein Endothelial Cell (HUVEC) growth on chitosan films and its enzymatically functionalized derivatives films with ferulic acid (FA) and ethyl ferulate (EF) was assessed by evaluating cell adhesion, morphology and cell viability. The results indicated that chitosan derivative films improved protein adsorption properties compared to chitosan films. The HUVEC cell morphology showed w ...
DOI:
10.1016/j.procbio.2014.02.001

28. Adhesion molecules from the diatom Phaeodactylum tricornutum (Bacillariophyceae): genomic identification by amino‐acid profiling and in vivo analysis

Author:
Anusuya Willis; Maeve Eason‐Hubbard; Oliver Hodson; Uma Maheswari; Chris Bowler; Richard Wetherbee; K. Valentin
Source:
Journal of phycology 2014 v.50 no.5 pp. 837-849
ISSN:
0022-3646
Subject:
Phaeodactylum tricornutum; adhesion; atomic force microscopy; bioinformatics; cell adhesion; eukaryotic cells; gene overexpression; genes; genetically modified organisms; lectins; phenotype; prokaryotic cells; quartz; turbulent flow; yellow fluorescent protein
Abstract:
... Cell adhesion molecules (CAMs) are important in prokaryotes and eukaryotes for cell–cell and cell–substratum interactions. The characteristics of adhesive proteins in the model diatom Phaeodactylum tricornutum were investigated by bioinformatic analysis and in vivo characterization. Bioinformatic analysis of the protein coding potential of the P. tricornutum genome used an amino‐acid profile that ...
DOI:
10.1111/jpy.12214

29. Adhesion of suspension cells on a coverslip in serum-free conditions

Author:
Tomofumi Nakayama; Keiko Mihara; Jin Kawata; Hiroshi Kimura; Hisato Saitoh
Source:
Analytical biochemistry 2014 v.466 pp. 1-3
ISSN:
0003-2697
Subject:
cell adhesion; cell lines; cost effectiveness; humans; microscopy
Abstract:
... Here, we present a rapid and damage-free fixation protocol for human cells cultured in suspension. Our results demonstrated that serum-free incubation of myeloid suspension cell lines HL-60, U937, and THP-1 for 10min resulted in cell adhesion to coverslips, allowing simple and efficient fixation for microscopy. The fixed cells exhibited an intact morphology and were suitable for immunostaining. Su ...
DOI:
10.1016/j.ab.2014.07.023

30. Adhesive forces and surface properties of cold gas plasma treated UHMWPE

Author:
Emily Callard Preedy; Emmanuel Brousseau; Sam L. Evans; Stefano Perni; Polina Prokopovich
Source:
Colloids and Surfaces A: Physicochemical and Engineering Aspects 2014 v.460 pp. 83-89
ISSN:
0927-7757
Subject:
adhesion; cell adhesion; cold; contact angle; crosslinking; friction; helium; hips; hydrophilicity; molecular weight; oxygen; polyethylene; prostheses; proteins
Abstract:
... Cold atmospheric plasma (CAP) treatment was used on ultra-high molecular weight polyethylene (UHMWPE), a common articulating counter material employed in hip and knee replacements. UHMWPE is a biocompatible polymer with low friction coefficient, yet does not have robust wear characteristics. CAP effectively cross-links the polymer chains of the UHMWPE improving wear performance (Perni et al., Acta ...
DOI:
10.1016/j.colsurfa.2014.03.052
PubMed:
25431523
PubMed Central:
PMC4236083

31. Advances in biofilm control for food and beverage industry using organo-silane technology: A review

Author:
Dorota Kregiel
Source:
Food control 2014 v.40 pp. 32-40
ISSN:
0956-7135
Subject:
adhesion; beverage industry; biofilm; biofouling; bioreactors; cell adhesion; corrosion; engineering; fermenters; food safety; laboratory experimentation
Abstract:
... Cell adhesion can be a potential problem as well as a valuable tool for microbiological engineering. It can lead to biofouling, contamination of product and corrosion. On the other hand, cell adhesion is purposely employed in fermenters and bioreactors to influence reactor performance. This paper presents an overview of organo-functional silanes – their chemistry, properties, use, and the main lab ...
DOI:
10.1016/j.foodcont.2013.11.014

32. Aging of Plasma-Polymerized Allylamine Nanofilms and the Maintenance of Their Cell Adhesion Capacity

Author:
Finke Birgit; Rebl Henrike; Hempel Frank; Schäfer Jan; Liefeith Klaus; Weltmann Klaus-Dieter; Nebe J. Barbara
Source:
Langmuir 2014 v.30 no.46 pp. 13914-13924
ISSN:
1520-5827
Subject:
Fourier transform infrared spectroscopy; Gibbs free energy; X-ray photoelectron spectroscopy; air; alloys; amides; cell adhesion; contact angle; free radicals; humans; osteoblasts; oxidation; oxygen; physicochemical properties; titanium; zeta potential
Abstract:
... The long-term stability and γ-sterilisability of bioactive layers is the precondition for the application of implants. Thus, aging processes of a microwave deposited, plasma polymerized allylamine nanofilm (PPAAm) with positively charged amino groups were evaluated concerning physicochemical characteristics and cell adhesion capacity over the course of one year. XPS, FT-IR, surface free energy, an ...
DOI:
10.1021/la5019778

33. Alanine-rich amphiphilic peptide containing the RGD cell adhesion motif: a coating material for human fibroblast attachment and culture

Author:
V. Castelletto; R. M. Gouveia; C. J. Connon; I. W. Hamley; J. Seitsonen; A. Nykänen; J. Ruokolainen
Source:
Biomaterials science 2014 v.2 no.3 pp. 362-369
ISSN:
2047-4849
Subject:
alanine; arginine; aspartic acid; cell adhesion; cell culture; cell proliferation; coatings; cornea; fibroblasts; humans; peptides; toxicity
Abstract:
... We studied the self-assembly of peptide A₆RGD (A: alanine, R: arginine, G: glycine, D: aspartic acid) in water, and the use of A₆RGD substrates as coatings to promote the attachment of human cornea stromal fibroblasts (hCSFs). The self-assembled motif of A₆RGD was shown to depend on the peptide concentration in water, where both vesicle and fibril formation were observed. Oligomers were detected f ...
DOI:
10.1039/c3bm60232j

34. Allosteric activation of ADAMTS13 by von Willebrand factor

Author:
Joshua Muia; Jian Zhu; Garima Gupta; Sandra L. Haberichter; Kenneth D. Friedman; Hendrik B. Feys; Louis Deforche; Karen Vanhoorelbeke; Lisa A. Westfield; Robyn Roth; Niraj Harish Tolia; John E. Heuser; J. Evan Sadler
Source:
Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.52 pp. 18584-18589
ISSN:
0027-8424
Subject:
adhesion; blood coagulation factors; blood platelets; cell adhesion; hemorrhage; metalloproteinases; thrombosis
Abstract:
... The metalloprotease ADAMTS13 cleaves von Willebrand factor (VWF) within endovascular platelet aggregates, and ADAMTS13 deficiency causes fatal microvascular thrombosis. The proximal metalloprotease (M), disintegrin-like (D), thrombospondin-1 (T), Cys-rich (C), and spacer (S) domains of ADAMTS13 recognize a cryptic site in VWF that is exposed by tensile force. Another seven T and two complement C1r ...
DOI:
10.1073/pnas.1413282112
PubMed:
25512528
PubMed Central:
PMC4284596

35. Alumina–zirconia composites functionalized with laminin-1 and laminin-5 for dentistry: Effect of protein adsorption on cellular response

Author:
A. Vallée; M.G. Faga; F. Mussano; F. Catalano; E. Tolosano; S. Carossa; F. Altruda; G. Martra
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.114 pp. 284-293
ISSN:
0927-7765
Subject:
X-ray photoelectron spectroscopy; adsorption; aesthetics; atomic force microscopy; basement membrane; cell adhesion; ceramics; colloids; dental materials; dentistry; epithelial cells; laminin; mechanical properties; phosphorylation; phosphotransferases (kinases)
Abstract:
... The present paper describes a study on laminin interaction with the surface of two alumina–zirconia composites with different percentages of ZrO2, both with submicrometric grain size. As major molecules within the basement membrane (BM), laminins are important protein fragments for epithelial cell adhesion and migration. On the other hand, alumina–zirconia composites are very attractive materials ...
DOI:
10.1016/j.colsurfb.2013.09.053

36. Amorphous calcium phosphate nanospheres/polylactide composite coated tantalum scaffold: Facile preparation, fast biomineralization and subchondral bone defect repair application

Author:
Rong Zhou; Wei Xu; Feng Chen; Chao Qi; Bing-Qiang Lu; Hao Zhang; Jin Wu; Qi-Rong Qian; Ying-Jie Zhu
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.123 pp. 236-245
ISSN:
0927-7765
Subject:
biomineralization; bovine serum albumin; calcium phosphates; cell adhesion; colloids; coprecipitation; drugs; gene transfer; humans; hydrophilicity; nanospheres; polylactic acid; rabbits; tantalum; tissue engineering; transforming growth factors; vascular endothelial growth factors
Abstract:
... Calcium phosphate (CaP) materials are widely used in various biomedical areas such as drug/gene delivery and bone repair/tissue engineering. In this study, amorphous CaP nanospheres synthesized by a simple co-precipitation method are used to prepare the CaP-polylactide (CaP-PLA) composite. Then, the as-prepared CaP-PLA composite is used to coat tantalum (Ta) plates and porous scaffolds. Compared w ...
DOI:
10.1016/j.colsurfb.2014.09.021

37. Amphiphilic beads as depots for sustained drug release integrated into fibrillar scaffolds

Author:
Akhilesh K. Gaharwar; Silvia M. Mihaila; Ashish A. Kulkarni; Alpesh Patel; Andrea Di Luca; Rui L. Reis; Manuela E. Gomes; Clemens van Blitterswijk; Lorenzo Moroni; Ali Khademhosseini
Source:
Journal of controlled release 2014 v.187 pp. 66-73
ISSN:
0168-3659
Subject:
alkaline phosphatase; bone formation; cell adhesion; composite polymers; dexamethasone; encapsulation; extracellular matrix; humans; hydrophobicity; mineralization; models; stem cells; tissue engineering; tissue repair
Abstract:
... Native extracellular matrix (ECM) is a complex fibrous structure loaded with bioactive cues that affects the surrounding cells. A promising strategy to mimicking native tissue architecture for tissue engineering applications is to engineer fibrous scaffolds using electrospinning. By loading appropriate bioactive cues within these fibrous scaffolds, various cellular functions such as cell adhesion, ...
DOI:
10.1016/j.jconrel.2014.04.035
PubMed:
24794894
PubMed Central:
PMC4079754

38. An Ancient Autoproteolytic Domain Found in GAIN, ZU5 and Nucleoporin98

Author:
Yuxing Liao; Jimin Pei; Hua Cheng; Nick V. Grishin
Source:
Journal of Molecular Biology 2014 v.426 pp. 3935-3945
ISSN:
0022-2836
Subject:
G-protein coupled receptors; adhesion; bacteria; cartilage; caspases; cell adhesion; evolution; global positioning systems; humans; polycystic kidney diseases; proteolysis; sequence homology
Abstract:
... A large family of G protein-coupled receptors (GPCRs) involved in cell adhesion has a characteristic autoproteolysis motif of HLT/S known as the GPCR proteolysis site (GPS). GPS is also shared by polycystic kidney disease proteins and it precedes the first transmembrane segment in both families. Recent structural studies have elucidated the GPS to be part of a larger domain named GPCR autoproteoly ...
DOI:
10.1016/j.jmb.2014.10.011
PubMed:
25451782
PubMed Central:
PMC4258472

39. An extract from medical leech improve the function of endothelial cells in vitro and attenuates atherosclerosis in ApoE null mice by reducing macrophages in the lesions

Author:
Yao Wang; Xin Zhao; Yun-Shan Wang; Shu-Liang Song; Hao Liang; Ai-Guo Ji
Source:
Biochemical and biophysical research communications 2014 v.455 pp. 119-125
ISSN:
0006-291X
Subject:
Oriental traditional medicine; adhesion; atherosclerosis; cell adhesion; chemotaxis; diet; dose response; endothelial cells; gene expression; humans; macrophages; messenger RNA; mice; monocytes; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Medicinal leech has been widely used as a traditional Chinese medicine in cardiovascular diseases. However, its pharmaceutical effect is not fully revealed. The goal of this study was to determine whether a leech extract has the effect of anti-atherosclerosis in ApoE −/− mice and the mechanism of this effect.In vivo experiments: ApoE −/− mice fed on high-cholesterol diet were separated into 5 grou ...
DOI:
10.1016/j.bbrc.2014.10.135

40. An in-depth comparison of the male pediatric and adult urinary proteomes

Author:
John W. Froehlich; Ali R. Vaezzadeh; Marc Kirchner; Andrew C. Briscoe; Oliver Hofmann; Winston Hide; Hanno Steen; Richard S. Lee
Source:
BBA - Proteins and Proteomics 2014 v.1844 pp. 1044-1050
ISSN:
1570-9639
Subject:
adults; biomarkers; cell adhesion; genes; immune response; infants; males; proteins; proteome; proteomics; transcription (genetics); translation (genetics); urine
Abstract:
... In this study, we performed an in-depth characterization of the male pediatric infant urinary proteome by parallel proteomic analysis of normal healthy adult (n=6) and infant (n=6) males and comparison to available published data. A total of 1584 protein groups were identified. Of these, 708 proteins were identified in samples from both cohorts. Although present in both cohorts, 136 of these commo ...
DOI:
10.1016/j.bbapap.2013.05.008
PubMed:
23707565
PubMed Central:
PMC3796057

41. Anabolic androgens affect the competitive interactions in cell migration and adhesion between normal mouse urothelial cells and urothelial carcinoma cells

Author:
Chi-Ping Huang; Teng-Fu Hsieh; Chi-Cheng Chen; Xiao-Fan Hung; Ai-Lin Yu; Chawnshang Chang; Chih-Rong Shyr
Source:
Biochemical and biophysical research communications 2014 v.452 pp. 322-327
ISSN:
0006-291X
Subject:
adhesion; androgens; cell adhesion; cell movement; coculture; mice; neoplasm cells; neoplasms; tissues; urine
Abstract:
... The urothelium is constantly rebuilt by normal urothelial cells to regenerate damaged tissues caused by stimuli in urine. However, the urothelial carcinoma cells expand the territory by aberrant growth of tumor cells, which migrate and occupy the damaged tissues to spread outside and disrupt the normal cells and organized tissues and form a tumor. Therefore, the interaction between normal urotheli ...
DOI:
10.1016/j.bbrc.2014.08.039

42. Angiopoietin-1 peptide QHREDGS promotes osteoblast differentiation, bone matrix deposition and mineralization on biomedical materials

Author:
Nicole T. Feric; Calvin C. H. Cheng; M. Cynthia Goh; Vyacheslav Dudnyk; Val Di Tizio; Milica Radisic
Source:
Biomaterials science 2014 v.2 no.10 pp. 1384-1398
ISSN:
2047-4849
Subject:
Fourier transform infrared spectroscopy; adhesion; alizarin; alkaline phosphatase; angiopoietin-1; atomic force microscopy; biocompatible materials; bone mineralization; bone resorption; bones; cardiomyocytes; cell adhesion; cell viability; coatings; endothelial cells; fibroblasts; gene expression; gene expression regulation; hydrogels; integrins; mineralization; osteoblasts; polyacrylic acid; polyethylene glycol; scanning electron microscopy; staining; synthetic peptides; therapeutics; titanium
Abstract:
... Bone loss occurs as a consequence of a variety of diseases as well as from traumatic injuries, and often requires therapeutic intervention. Strategies for repairing and replacing damaged and/or lost bone tissue include the use of biomaterials and medical implant devices with and without osteoinductive coatings. The soluble growth factor angiopoietin-1 (Ang-1) has been found to promote cell adhesio ...
DOI:
10.1039/c4bm00073k
PubMed:
25485104
PubMed Central:
PMC4251595

43. Anthocyanins from fruit juices improve the antioxidant status of healthy young female volunteers without affecting anti-inflammatory parameters: results from the randomised, double-blind, placebo-controlled, cross-over ANTHONIA (ANTHOcyanins in Nutrition Investigation Alliance) study

Author:
Sabine Kuntz; Clemens Kunz; Johannes Herrmann; Christian H. Borsch; Georg Abel; Bettina Fröhling; Helmut Dietrich; Silvia Rudloff
Source:
British journal of nutrition 2014 v.112 no.6 pp. 925-936
ISSN:
1475-2662
Subject:
anthocyanins; antioxidant activity; antioxidants; atherosclerosis; beverages; catalase; cell adhesion; chemokine CCL2; cross-over studies; enzyme activity; erythrocytes; females; fruit juices; glutathione peroxidase; ingestion; interleukin-2; malondialdehyde; nutrition; oxidative stress; placebos; superoxide dismutase; tumor necrosis factor-alpha; urine; volunteers
Abstract:
... Anthocyanins (ACN) can exert beneficial health effects not only through their antioxidative potential but also through modulation of inflammatory parameters that play a major role in CVD. A randomised cross-over study was carried out to investigate the effects of ACN-rich beverage ingestion on oxidation- and inflammation-related parameters in thirty healthy female volunteers. The participants cons ...
DOI:
10.1017/S0007114514001482
PubMed:
25089359

44. Anti-VCAM-1 SAINT-O-Somes enable endothelial-specific delivery of siRNA and downregulation of inflammatory genes in activated endothelium in vivo

Author:
Piotr S. Kowalski; Peter J. Zwiers; Henriëtte W.M. Morselt; Joanna M. Kuldo; Niek G.J. Leus; Marcel H.J. Ruiters; Grietje Molema; Jan A.A.M. Kamps
Source:
Journal of controlled release 2014 v.176 pp. 64-75
ISSN:
0168-3659
Subject:
antibodies; cell adhesion; endothelial cells; endothelium; genes; inflammation; intravenous injection; kidneys; lipopolysaccharides; liver; messenger RNA; mice; nephrotoxicity; small interfering RNA; surfactants; transcription factor NF-kappa B; tumor necrosis factor-alpha; vascular cell adhesion molecules
Abstract:
... The pivotal role of endothelial cells in the pathology of inflammatory diseases raised interest in the development of short interfering RNA (siRNA) delivery devices for selective pharmacological intervention in the inflamed endothelium. The current study demonstrates endothelial specific delivery of siRNAs and downregulation of inflammatory genes in activated endothelium in vivo by applying a nove ...
DOI:
10.1016/j.jconrel.2013.12.029

45. Anti-angiogenic activities of two recombinant disintegrins derived from the Mohave and Prairie rattlesnakes

Author:
Sara E. Lucena; Karen Romo; Montamas Suntravat; Elda E. Sánchez
Source:
Toxicon 2014 v.78 pp. 10-17
ISSN:
0041-0101
Subject:
adhesion; amino acids; angiogenesis; blood vessels; cell adhesion; cell proliferation; endothelial cells; fibronectins; integrins; neoplasm cells; neoplasms; nutrients; oxygen; peptides; platelet aggregation; receptors; snakes; therapeutics; venoms
Abstract:
... Angiogenesis plays a crucial role in the growth and spread of cancer. New vascularization nourishes cancer cells with oxygen and nutrients, allowing these cells to grow, invade nearby tissue, spread to other parts of the body, and form new colonies of cancer cells. Tumor angiogenesis consists of endothelial cell proliferation, migration, and tube formation into the tumor mass. The study of natural ...
DOI:
10.1016/j.toxicon.2013.11.005
PubMed:
24269784
PubMed Central:
PMC4270208

46. Anti-biofouling ability and cytocompatibility of the zwitterionic brushes-modified cellulose membrane

Author:
Pingsheng Liu; Qiang Chen; Li Li; Sicong Lin; Jian Shen
Source:
Journal of materials chemistry B 2014 v.2 no.41 pp. 7222-7231
ISSN:
2050-7518
Subject:
X-ray photoelectron spectroscopy; adhesion; adsorption; biocompatibility; biocompatible materials; blood platelets; cell adhesion; cell culture; cell structures; cellulose; coatings; encapsulation; hydrogels; hydrophilicity; polyethylene glycol; polymerization; proteins; zwitterions
Abstract:
... Blood compatibility and cytocompatibility are key requirements of blood-contacting biomaterials for biomedical applications. Here, we showed the general zwitterionic surface modification of cellulose membrane (CM) substrates, via surface-initiated atom transfer radical polymerization (SI-ATRP), to enhance the anti-biofouling ability without compromising the cytocompatibility of the substrates. Rob ...
DOI:
10.1039/c4tb01151a

47. Anti-septic effects of glyceollins in HMGB1-induced inflammatory responses in vitro and in vivo

Author:
Wonhwa Lee; Sae-Kwang Ku; You-Mie Lee; Jong-Sup Bae
Source:
Food and chemical toxicology 2014 v.63 pp. 1-8
ISSN:
0278-6915
Subject:
Aspergillus sojae; active ingredients; adhesion; anti-inflammatory activity; antiseptics; cell adhesion; cell movement; human umbilical vein endothelial cells; inflammation; interleukin-6; macrophages; mice; necrosis; nucleoproteins; phosphotransferases (kinases); sepsis (infection); signal transduction; toxicology; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... The ubiquitous nuclear protein High mobility group box 1 (HMGB1) is released by activated macrophages and human umbilical vein endothelial cells (HUVECs), and functions as a late mediator of experimental sepsis. Glyceollins (GCLs) are active compounds from Aspergillus sojae which have been reported for anti-cancer, anti-diabetes, and anti-inflammatory activities. We investigated here, the antisept ...
DOI:
10.1016/j.fct.2013.10.034

48. Antibiofilm formation and anti‐adhesive (to HEp‐2 cells) effects of rosemary water extract against some food‐related pathogens

Author:
Hesham Elhariry; Abeer A. Abuzaid; Ghada M. Khiralla; Youssuf Gherbawy
Source:
International journal of food science & technology 2014 v.49 no.4 pp. 1132-1141
ISSN:
0950-5423
Subject:
Pseudomonas; acids; bacteria; cell adhesion; daidzein; genistein; high performance liquid chromatography; pathogens; phenolic compounds; rosemary
Abstract:
... The present work aimed to determine the bioactive compounds in two rosemary water extracts (RWE1 and RWE2) and to assess their antimicrobial, anti‐adhesive and antibiofilm potentials against the food‐related Bacillus and Pseudomonas species at concentrations; 4, 8, 12, 16 and 20 mg mL⁻¹. Phenolic compounds and isoflavones in the RWEs were determined using HPLC. The concentrations of most bioactive ...
DOI:
10.1111/ijfs.12409

49. Antibiotic-Loaded Chitosan Hydrogel with Superior Dual Functions: Antibacterial Efficacy and Osteoblastic Cell Responses

Author:
Wu Fang; Meng Guolong; He Jing; Wu Yao; Gu Zhongwei
Source:
ACS Applied Materials & Interfaces 2014 v.6 no.13 pp. 10005-10013
ISSN:
1944-8252
Subject:
Staphylococcus aureus; adhesion; antibacterial properties; bacteria; biocompatibility; biocompatible materials; biofilm; cell adhesion; chitosan; crosslinking; gentamicin; hydrogels; membrane permeability; orthopedics; sulfates
Abstract:
... It is critical for the clinical success to take the biological function into consideration when integrating the antibacterial function into the implanted biomaterials. To this aim, we prepared gentamycin sulfate (GS)-loaded carboxymethyl-chitosan (CM-chitosan) hydrogel cross-linked by genipin. The prepared hydrogels not only achieved superb inhibition on bacteria growth and biofilm formation of St ...
DOI:
10.1021/am502537k

50. Antitumor activity of the dietary diterpene carnosol against a panel of human cancer cell lines

Author:
Daniele Vergara; Pasquale Simeone; Simona Bettini; Andrea Tinelli; Ludovico Valli; Carlo Storelli; Silvana Leo; Angelo Santino; Michele Maffia
Source:
Food & function 2014 v.5 no.6 pp. 1261-1269
ISSN:
2042-650X
Subject:
animal ovaries; anticarcinogenic activity; antineoplastic activity; ascites; bioactive compounds; breasts; cell adhesion; cell viability; curcumin; diterpenoids; drug therapy; drugs; epithelium; fibronectins; fruits; human cell lines; humans; intestines; metastasis; neoplasm cells; ovarian neoplasms; patients; phytochemicals; vegetables
Abstract:
... Dietary phytochemicals found in vegetables and fruits consist of a wide variety of biologically active compounds with anti-carcinogenic activity. The aim of this study was to evaluate the antigrowth activity of carnosol, a dietary diterpene, as a single agent or in combination with other dietary phytochemicals or chemotherapeutic drugs against a panel of tumor cell lines. Carnosol decreased cell v ...
DOI:
10.1039/c4fo00023d

51. Aquaporin 0 plays a pivotal role in refractive index gradient development in mammalian eye lens to prevent spherical aberration

Author:
S. Sindhu Kumari; Kulandaiappan Varadaraj
Source:
Biochemical and biophysical research communications 2014 v.452 pp. 986-991
ISSN:
0006-291X
Subject:
animal models; aquaporins; cataract; cell adhesion; cell membranes; cortex; extracellular space; eye lens; fiber cells; genetically modified organisms; heterozygosity; homeostasis; in vitro studies; light scattering; matrix-assisted laser desorption-ionization mass spectrometry; mice; mutation; permeability; polyacrylamide gel electrophoresis; refractive index; transgenes; water content
Abstract:
... Aquaporin 0 (AQP0) is a transmembrane channel that constitutes ∼45% of the total membrane protein of the fiber cells in mammalian lens. It is critical for lens transparency and homeostasis as mutations and knockout cause autosomal dominant lens cataract. AQP0 functions as a water channel and as a cell-to-cell adhesion (CTCA) molecule in the lens. Our recent in vitro studies showed that the CTCA fu ...
DOI:
10.1016/j.bbrc.2014.09.032
PubMed:
25229686
PubMed Central:
PMC4209735

52. Are the basal cells of the mammalian epididymis still an enigma?

Author:
S. Arrighi
Source:
Reproduction, fertility, and development 2014 v.26 no.8 pp. 1061-1071
ISSN:
1031-3613
Subject:
antigens; aquaporins; cell adhesion; electrolytes; epididymis; epithelium; glycerol; macrophages; prostaglandins; rats; spermatozoa; testes
Abstract:
... Basal cells are present in the columnar pseudostratified epithelium covering the epididymis of all mammalian species, which regulates the microenvironment where the functionally incompetent germ cells produced by the testis are matured and stored. Striking novelties have come from investigations on epididymal basal cells in the past 30–40 years. In addition to an earlier hypothesised scavenger rol ...
DOI:
10.1071/RD13301
PubMed:
24138802

53. Arrhythmogenic cardiomyopathy and Brugada syndrome: Diseases of the connexome

Author:
Esperanza Agullo-Pascual; Marina Cerrone; Mario Delmar
Source:
FEBS letters 2014 v.588 no.8 pp. 1322-1330
ISSN:
0014-5793
Subject:
arrhythmia; cardiomyopathy; cell adhesion; desmosomes; gap junctions; heart; mutation; sodium channels; translation (genetics)
Abstract:
... This review summarizes data in support of the notion that the cardiac intercalated disc is the host of a protein interacting network, called “the connexome”, where molecules classically defined as belonging to one particular structure (e.g., desmosomes, gap junctions, sodium channel complex) actually interact with others, and together, control excitability, electrical coupling and intercellular ad ...
DOI:
10.1016/j.febslet.2014.02.008
PubMed:
24548564
PubMed Central:
PMC3989410

54. Assay for characterizing the recovery of vertebrate cells for adhesion measurements by single-cell force spectroscopy

Author:
Rajib Schubert; Nico Strohmeyer; Mitasha Bharadwaj; Subramanian P. Ramanathan; Michael Krieg; Jens Friedrichs; Clemens M. Franz; Daniel J. Muller
Source:
FEBS letters 2014 v.588 no.19 pp. 3639-3648
ISSN:
0014-5793
Subject:
EDTA (chelating agent); adhesion; atomic force microscopy; cell adhesion; culture flasks; fibroblasts; kidneys; mammals; spectroscopy; tissue culture; trypsin
Abstract:
... Single-cell force spectroscopy (SCFS) is becoming a widely used method to quantify the adhesion of a living cell to a substrate, another cell or tissue. The high sensitivity of SCFS permits determining the contributions of individual cell adhesion molecules (CAMs) to the adhesion force of an entire cell. However, to prepare adherent cells for SCFS, they must first be detached from tissue-culture f ...
DOI:
10.1016/j.febslet.2014.06.012

55. Assembly, postsynthetic modification and hepatocyte targeting by multiantennary, galactosylated soft structures

Author:
Anisha Thomas; Akansha Shukla; Sri Sivakumar; Sandeep Verma
Source:
Chemical communications 2014 v.50 no.99 pp. 15752-15755
ISSN:
1364-548X
Subject:
aldehydes; cell adhesion; chemical reactions; crosslinking; galactose; lysine; moieties
Abstract:
... Enzyme modifiable, hollow self-assembled structures offer an excellent scope for multiantennary delivery vectors. Herein, we report synthesis and applications of bis-galactose lysine based supramolecular ensembles, which possess surface galactose moieties amenable to enzymatic modifications. This post-synthetic modification generates reactive aldehyde groups, which could possibly serve as dynamic ...
DOI:
10.1039/c4cc07074g

56. Atomic layer deposition enhanced grafting of phosphorylcholine on stainless steel for intravascular stents

Author:
Qi Zhong; Jin Yan; Xu Qian; Tao Zhang; Zhuo Zhang; Aidong Li
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.121 pp. 238-247
ISSN:
0927-7765
Subject:
X-ray photoelectron spectroscopy; adhesion; adsorption; aluminum oxide; blood platelets; cell adhesion; cell membranes; cell proliferation; coatings; colloids; contact angle; drugs; human umbilical vein endothelial cells; industry; medical equipment; polymers; semiconductors; stainless steel
Abstract:
... In-stent restenosis (ISR) and re-endothelialization delay are two major issues of intravascular stent in terms of clinical safety and effects. Construction of mimetic cell membrane surface on stents using phosphorylcholine have been regarded as one of the most powerful strategies to resolve these two issues and improve the performance of stents. In this study, atomic layer deposition (ALD) technol ...
DOI:
10.1016/j.colsurfb.2014.06.022

57. Bauhinia forficata lectin (BfL) induces cell death and inhibits integrin-mediated adhesion on MCF7 human breast cancer cells

Author:
Mariana C.C. Silva; Cláudia A.A. de Paula; Joana G. Ferreira; Edgar J. Paredes-Gamero; Angela M.S.F. Vaz; Misako U. Sampaio; Maria Tereza S. Correia; Maria Luiza V. Oliva
Source:
BBA - General Subjects 2014 v.1840 pp. 2262-2271
ISSN:
0304-4165
Subject:
Bauhinia forficata; CD29 antigen; DNA; adhesion; antineoplastic activity; breast neoplasms; caspase-9; cell adhesion; cell cycle checkpoints; collagen; cytotoxicity; death; fibronectins; flow cytometry; glycoproteins; glycosylation; humans; laminin; lectins; necrosis; neoplasm cells; regulatory proteins; seeds; viability; women
Abstract:
... Plant lectins have attracted great interest in cancer studies due to their antitumor activities. These proteins or glycoproteins specifically and reversibly bind to different types of carbohydrates or glycoproteins. Breast cancer, which presents altered glycosylation of cell surface glycoproteins, is one of the most frequent malignant diseases in women. In this work, we describe the effect of the ...
DOI:
10.1016/j.bbagen.2014.03.009

58. Bi-ligand surfaces with oriented and patterned protein for real-time tracking of cell migration

Author:
Varadraj N. Vernekar; Charles S. Wallace; Mina Wu; Joshua T. Chao; Shannon K. O’Connor; Aimee Raleigh; Xiaji Liu; Jason M. Haugh; William M. Reichert
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.123 pp. 225-235
ISSN:
0927-7765
Subject:
B-lymphocytes; X-ray photoelectron spectroscopy; active sites; antibodies; cell adhesion; cell movement; chemokines; chemotaxis; colloids; mice; receptors; recombinant proteins; silica; staining; surface proteins; ultraviolet radiation
Abstract:
... A bioactive platform for the quantitative observation of cell migration is presented by (1) presenting migration factors in a well-defined manner on 2-D substrates, and (2) enabling continuous cell tracking. Well-defined substrate presentation is achieved by correctly orienting immobilized proteins (chemokines and cell adhesion molecules), such that the active site is accessible to cell surface re ...
DOI:
10.1016/j.colsurfb.2014.09.020
PubMed:
25262410
PubMed Central:
PMC4259856

59. Bienzymatically crosslinked gelatin/hyaluronic acid interpenetrating network hydrogels: preparation and characterization

Author:
Zhiping Fan; Yemin Zhang; Shuo Fang; Chen Xu; Xinsong Li
Source:
RSC advances 2014 v.5 no.3 pp. 1929-1936
ISSN:
2046-2069
Subject:
biocompatibility; biocompatible materials; biological assessment; biopolymers; cell adhesion; compression strength; crosslinking; cytotoxicity; extracellular matrix; gelatin; gelation; hyaluronic acid; hydrogels; peroxidase; protein-glutamine gamma-glutamyltransferase; rheometers; strength (mechanics); tissue engineering; tyramine; water content
Abstract:
... Hydrogels that mimic the extracellular matrix in both composition and crosslinking chemistry have great potential in the development of tissue engineering scaffolds. Natural polymer hydrogels crosslinked by enzymes have excellent biocompatibility due to their natural origin and the mild reaction conditions, but usually suffer from a lack of mechanical strength. In order to improve the mechanical p ...
DOI:
10.1039/c4ra12446d

60. Bile carcinoembryonic cell adhesion molecule 6 (CEAM6) as a biomarker of malignant biliary stenoses

Author:
Annarita Farina; Jean-Marc Dumonceau; Paola Antinori; Isabelle Annessi-Ramseyer; Jean-Louis Frossard; Denis F. Hochstrasser; Myriam Delhaye; Pierre Lescuyer
Source:
BBA - Proteins and Proteomics 2014 v.1844 pp. 1018-1025
ISSN:
1570-9639
Subject:
bile; biomarkers; blood serum; cell adhesion; enzyme-linked immunosorbent assay; humans; neoplasms; patients; proteomics; screening; surface proteins
Abstract:
... Differentiating malignant from nonmalignant biliary stenoses is challenging. This could be facilitated by the measurement of cancer biomarkers in bile. We aimed at (i) identifying new cancer biomarkers by comparative proteomic analysis of bile collected from patients with a malignant or benign biliary stenosis (exploratory phase) and (ii) verifying the accuracy of the newly identified potential bi ...
DOI:
10.1016/j.bbapap.2013.06.010

61. Bioactive nanocomposites of bacterial cellulose and natural hydrocolloids

Author:
Marco Aurelio Woehl; Lucy Ono; Izabel Cristina Riegel Vidotti; Fernando Wypych; Wido Herwig Schreiner; Maria Rita Sierakowski
Source:
Journal of materials chemistry B 2014 v.2 no.40 pp. 7034-7044
ISSN:
2050-7518
Subject:
X-ray photoelectron spectroscopy; atomic force microscopy; cell adhesion; cell growth; cellulose; coatings; collagen; cost effectiveness; dipping; dispersions; drugs; green chemistry; guar gum; hyaluronic acid; hydrocolloids; mechanical methods; nanocomposites; nanofibers; purification methods; solvents; swelling (materials); tissues; toxicity
Abstract:
... The aim of this work was to develop bioactive films from bacterial cellulose and hydrocolloids (guar gum and hyaluronic acid), coated or not with collagen. After mechanical treatment, a suspension of cellulose nanofibres was obtained which, combined with the dispersions of hydrocolloids, was used to produce bionanocomposite films by wet casting. The materials were stable in physiological solution ...
DOI:
10.1039/c4tb00706a

62. Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties

Author:
Sandra Ullm; Anne Krüger; Christoph Tondera; Tim P. Gebauer; Axel T. Neffe; Andreas Lendlein; Friedrich Jung; Jens Pietzsch
Source:
Biomaterials 2014 v.35 pp. 9755-9766
ISSN:
0142-9612
Subject:
biocompatibility; biocompatible materials; cell adhesion; cytokines; endothelial cells; gelatin; granulocytes; humans; hydrogels; inflammation; lysine; macrophages; metalloproteinases; mice; prostaglandin synthase; secretion; tight junctions; tissues
Abstract:
... Hydrogels prepared from gelatin and lysine diisocyanate ethyl ester provide tailorable elastic properties and degradation behavior. Their interaction with human aortic endothelial cells (HAEC) as well as human macrophages (Mɸ) and granulocytes (Gɸ) were explored. The experiments revealed a good biocompatibility, appropriate cell adhesion, and cell infiltration. Direct contact to hydrogels, but not ...
DOI:
10.1016/j.biomaterials.2014.08.023

63. Biohydrogels Interpenetrated with Hydroxyethyl Cellulose and Wooden Pulp for Biocompatible Materials

Author:
Tovar-Carrillo Karla Lizette; Tagaya Motohiro; Kobayashi Takaomi
Source:
Industrial & Engineering Chemistry Research 2014 v.53 no.12 pp. 4650-4659
ISSN:
1520-5045
Subject:
adhesion; adsorption; biocompatibility; biocompatible materials; blood platelets; cell adhesion; cellulose; engineering; fibroblasts; films (materials); hydrogels; mechanical properties; polystyrenes; pulp; tissue culture
Abstract:
... Biohydrogel interpenetrated films of wooden pulp cellulose and hydroxyethyl cellulose (HEC) were prepared, and their biomedical properties were investigated. Our purpose was to obtain new material combining the nontoxicity and biocompatibility in wooden pulp cellulose films by interpenetration of HEC. It was found that the increment of HEC content affects the mechanical properties of the hydrogel ...
DOI:
10.1021/ie403257a

64. Bioinspired Microfluidic Assay for In Vitro Modeling of Leukocyte–Endothelium Interactions

Author:
Lamberti Giuseppina; Prabhakarpandian Balabhaskar; Garson Charles; Smith Ashley; Pant Kapil; Wang Bin; Kiani Mohammad F.
Source:
Analytical chemistry 2014 v.86 no.16 pp. 8344-8351
ISSN:
1520-6882
Subject:
cell adhesion; endothelial cells; humans; in vitro studies; models; monoclonal antibodies; neutrophils; rapid methods; rolling; screening
Abstract:
... Current in vitro models of the leukocyte adhesion cascade cannot be used for real-time studies of the entire leukocyte adhesion cascade, including rolling, adhesion, and migration in a single assay. In this study, we have developed and validated a novel bioinspired microfluidic assay (bMFA) and used it to test the hypothesis that blocking of specific steps in the adhesion/migration cascade signifi ...
DOI:
10.1021/ac5018716
PubMed:
25135319
PubMed Central:
PMC4139165

65. Biointerfaces on Indium–Tin Oxide Prepared from Organophosphonic Acid Self-Assembled Monolayers

Author:
Chockalingam Muthukumar; Magenau Astrid; Parker Stephen G.; Parviz Maryam; Vivekchand S. R. C.; Gaus Katharina; Gooding J. Justin
Source:
Langmuir 2014 v.30 no.28 pp. 8509-8515
ISSN:
1520-5827
Subject:
cell adhesion; ethylene oxide; gold; indium tin oxide; ligands; silicon
Abstract:
... Herein we show the development of biointerfaces on indium–tin oxide (ITO) surfaces prepared from organophosphonate self-assembled monolayers. The interfaces were prepared in a stepwise fabrication procedure containing a base monolayer modified with oligo(ethylene oxide) species to which biological recognition ligands were attached. The density of ligands was controlled by varying the ratio of two ...
DOI:
10.1021/la501774b

66. Biological properties of novel chitosan-based composites for medical application as bone substitute

Author:
Agata Przekora; Grażyna Ginalska
Source:
Open life sciences 2014 v.9 no.6 pp. 634-641
ISSN:
2391-5412
Subject:
biocompatibility; bone substitutes; bones; cell adhesion; chitosan; cytotoxicity; humans; hydroxyapatite; in vitro studies; krill; mechanical properties; medicine; teeth
Abstract:
... Hydroxyapatite is the main inorganic component of bones and teeth. In order to improve mechanical properties and surgical handiness of bioceramics, a plasticizing agent e.g. polysaccharide can be added. Chitosan is a polysaccharide with biological properties that make it an ideal component of bioceramics-based composites for medical application as bone substitute. In this study, biocompatibility o ...
DOI:
10.2478/s11535-014-0297-y

67. Biomaterial arrays with defined adhesion ligand densities and matrix stiffness identify distinct phenotypes for tumorigenic and non-tumorigenic human mesenchymal cell types

Author:
Tyler D. Hansen; Justin T. Koepsel; Ngoc Nhi Le; Eric H. Nguyen; Stefan Zorn; Matthew Parlato; Samuel G. Loveland; Michael P. Schwartz; William L. Murphy
Source:
Biomaterials science 2014 v.2 no.5 pp. 745-756
ISSN:
2047-4849
Subject:
adhesion; biocompatible materials; cell adhesion; cell lines; fibrosarcoma; humans; hydrogels; ligands; mechanical properties; mesenchymal stromal cells; models; modulus of elasticity; neoplasm cells; peptides; phenotype; polyethylene glycol
Abstract:
... Here, we aimed to investigate migration of a model tumor cell line (HT-1080 fibrosarcoma cells, HT-1080s) using synthetic biomaterials to systematically vary peptide ligand density and substrate stiffness. A range of substrate elastic moduli were investigated by using poly(ethylene glycol) (PEG) hydrogel arrays (0.34–17 kPa) and self-assembled monolayer (SAM) arrays (∼0.1–1 GPa), while cell adhesi ...
DOI:
10.1039/c3bm60278h
PubMed:
25386339
PubMed Central:
PMC4224020

68. Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

Author:
Lena A. Lautscham; Corey Y. Lin; Vera Auernheimer; Christoph A. Naumann; Wolfgang H. Goldmann; Ben Fabry
Source:
Biomaterials 2014 v.35 pp. 3198-3207
ISSN:
0142-9612
Subject:
cell adhesion; extracellular matrix; fibroblasts; glass; ligands; lipid bilayers; mechanical properties; polyacrylamide; viscoelasticity
Abstract:
... Cell behavior such as cell adhesion, spreading, and contraction critically depends on the elastic properties of the extracellular matrix. It is not known, however, how cells respond to viscoelastic or plastic material properties that more closely resemble the mechanical environment cells encounter in the body. In this report, we employ viscoelastic and plastic biomembrane-mimicking cell substrates ...
DOI:
10.1016/j.biomaterials.2013.12.091
PubMed:
24439398
PubMed Central:
PMC4026006

69. Biomimetic Design of Platelet Adhesion Inhibitors to Block Integrin α2β1-Collagen Interactions: I. Construction of an Affinity Binding Model

Author:
Zhang Lin; Sun Yan
Source:
Langmuir 2014 v.30 no.16 pp. 4725-4733
ISSN:
1520-5827
Subject:
Gibbs free energy; adhesion; biomimetics; blood platelets; cell adhesion; collagen; drugs; hydrophobic bonding; integrins; models; molecular dynamics; surface area; thrombosis
Abstract:
... Platelet adhesion on a collagen surface through integrin α2β1 has been proven to be significant for the formation of arterial thrombus. However, the molecular determinants mediating the integrin–collagen complex remain unclear. In the present study, the dynamics of integrin–collagen binding and molecular interactions were investigated using molecular dynamics (MD) simulations and molecular mechani ...
DOI:
10.1021/la404599s

70. Biomimetic Design of Platelet Adhesion Inhibitors to Block Integrin α2β1-Collagen Interactions: II. Inhibitor Library, Screening, and Experimental Validation

Author:
Zhang Lin; Zhang Chao; Sun Yan
Source:
Langmuir 2014 v.30 no.16 pp. 4734-4742
ISSN:
1520-5827
Subject:
adhesion; binding capacity; biomimetics; blood platelets; calorimetry; cell adhesion; collagen; integrins; models; molecular dynamics; screening; thrombosis; titration
Abstract:
... Platelet adhesion on collagen mediated by integrin α2β1 has been proven important in arterial thrombus formation, leading to an exigent demand on development of potent inhibitors for the integrin α2β1-collagen binding. In the present study, a biomimetic design strategy of platelet adhesion inhibitors was established, based on the affinity binding model of integrin proposed in part I. First, a hept ...
DOI:
10.1021/la4046012

71. Biomimetic Nucleation of Hydroxyapatite Crystals Mediated by Antheraea pernyi Silk Sericin Promotes Osteogenic Differentiation of Human Bone Marrow Derived Mesenchymal Stem Cells

Author:
Yang Mingying; Shuai Yajun; Zhang Can; Chen Yuyin; Zhu Liangjun; Mao Chuanbin; OuYang Hongwei
Source:
Biomacromolecules 2014 v.15 no.4 pp. 1185-1193
ISSN:
1526-4602
Subject:
Antheraea pernyi; biocompatible materials; biodegradation; biomimetics; biomineralization; bone formation; bone marrow; cell adhesion; cell viability; crystals; humans; hydrophilicity; hydroxyapatite; sericin; silk; silkworms; stem cells; tissue engineering
Abstract:
... Biomacromolecules have been used as templates to grow hydroxyapatite crystals (HAps) by biomineralization to fabricate mineralized materials for potential application in bone tissue engineering. Silk sericin is a protein with features desirable as a biomaterial, such as increased hydrophilicity and biodegradation. Mineralization of the silk sericin from Antheraea pernyi (A. pernyi) silkworm has ra ...
DOI:
10.1021/bm401740x
PubMed:
24666022
PubMed Central:
PMC3993896

72. Biomimetic honeycomb-patterned surface as the tunable cell adhesion scaffold

Author:
Shuangshuang Chen; Xuemin Lu; Ying Hu; Qinghua Lu
Source:
Biomaterials science 2014 v.3 no.1 pp. 85-93
ISSN:
2047-4849
Subject:
Parthenocissus tricuspidata; antifouling activities; betaine; bioadhesion; bioadhesives; biocompatible materials; biomimetics; cell adhesion; fish skin; mucus; polystyrenes; zwitterions
Abstract:
... Inspired by the typically adhesive behaviors of fish skin and Parthenocissus tricuspidata, two different decorations of polystyrene honeycomb membrane (PSHCM) prepared by the breath figure approach were carried out with poly(N-(3-Sulfopropyl)-N-(methacryloxyethyl)-N,N-dimethylammonium betaine)(polySBMA) to explore controllable bioadhesive surfaces. Casting and dip-coating were employed to graft po ...
DOI:
10.1039/c4bm00233d

73. Bionic design for surface optimization combining hydrophilic and negative charged biological macromolecules

Author:
Fen Ran; Haiming Song; Xiaoqin Niu; Aimei Yang; Shengqiang Nie; Lingren Wang; Jie Li; Shudong Sun; Changsheng Zhao
Source:
International journal of biological macromolecules 2014 v.67 pp. 260-269
ISSN:
0141-8130
Subject:
adhesion; blood coagulation; blood platelets; cell adhesion; coatings; heparin; hydrophilicity; mixing; polymerization; pyrrolidones; styrene
Abstract:
... While polyethersulfone (PES) membrane represents a promising option for blood purification, the blood compatibility must be dramatically enhanced to meet today's ever-increasing demands for many emerging application. In this study, we report a bionic design for optimization and development of a modified PES membrane combining hydrophilic and negative charged biological macromolecules on its surfac ...
DOI:
10.1016/j.ijbiomac.2014.03.034

74. Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals

Author:
Chin Fhong Soon; Wan Ibtisam Wan Omar; Rebecca F. Berends; Nafarizal Nayan; Hatijah Basri; Kian Sek Tee; Mansour Youseffi; Nick Blagden; Morgan Clive Thomas Denyer
Source:
Micron 2014 v.56 pp. 73-79
ISSN:
0968-4328
Subject:
Fourier transform infrared spectroscopy; atomic force microscopy; cell adhesion; cell culture; cell membranes; cholesterol; collagen; culture media; cultured cells; extracellular matrix; fibronectins; glass; humans; image analysis; integrins; keratinocytes; laminin; liquid crystals; physicochemical properties; surface roughness
Abstract:
... This study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocyto ...
DOI:
10.1016/j.micron.2013.10.011

75. Blood activation and compatibility on single-molecular-layer biointerfaces

Author:
Shengqiang Nie; Hui Qin; Chong Cheng; Weifeng Zhao; Shudong Sun; Baihai Su; Changsheng Zhao; Zhongwei Gu
Source:
Journal of materials chemistry B 2014 v.2 no.30 pp. 4911-4921
ISSN:
2050-7518
Subject:
X-ray photoelectron spectroscopy; adhesion; adsorption; atomic force microscopy; benzene; biocompatibility; biocompatible materials; blood platelets; cell adhesion; cell proliferation; complement; contact angle; drugs; encapsulation; hydrophilicity; hydrophobicity; moieties; silicon; stem cells; tissue culture
Abstract:
... Research on the interactions between living systems and materials is fuelled by diverse biomedical needs, for example, drug encapsulation and stimulated release, stem cell proliferation and differentiation, cell and tissue cultures, as well as artificial organs. Specific single-molecular-layer biointerface design is one of the most important processes to reveal the interactions or biological respo ...
DOI:
10.1039/c4tb00555d

76. Blood compatibility and interaction with endothelial cells of titanium modified by sequential immobilization of poly (ethylene glycol) and heparin

Author:
Chang-Jiang Pan; Yan-Hua Hou; Bin-Bin Zhang; Yun-Xiao Dong; Hong-Yan Ding
Source:
Journal of materials chemistry B 2014 v.2 no.7 pp. 892-902
ISSN:
2050-7518
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adsorption; biocompatibility; biocompatible materials; blood platelets; cell adhesion; endothelial cells; fibrinogen; heparin; humans; hydrophilicity; platelet activation; polyethylene glycol; scanning electron microscopy; thromboplastin; titanium
Abstract:
... In this study, poly(ethylene glycol) (PEG) and heparin were sequentially immobilized on a titanium surface by the carbodiimide covalent coupling method with the aim to improve the blood compatibility of titanium and enhance endothelial cell adhesion and proliferation. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy ...
DOI:
10.1039/c3tb21403f

77. Blood compatibility comparison for polysulfone membranes modified by grafting block and random zwitterionic copolymers via surface-initiated ATRP

Author:
Tao Xiang; Li-Sha Zhang; Rui Wang; Yi Xia; Bai-Hai Su; Chang-Sheng Zhao
Source:
Journal of colloid and interface science 2014 v.432 pp. 47-56
ISSN:
0021-9797
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adhesion; adsorption; anticoagulant activity; biocompatibility; blood platelets; cell adhesion; chemical bonding; chemical composition; composite polymers; contact angle; hemodialysis; hydrophilicity; polymerization; scanning electron microscopy; sulfobetaine; zwitterions
Abstract:
... For blood-contacting materials, good blood compatibility, especially good anticoagulant property is of great importance. Zwitterionic polymers have been proved to be resistant to nonspecific protein adsorption and platelet adhesion; however, their anticoagulant property is always inadequate. In this study, two kinds of zwitterionic copolymers (sulfobetaine methacrylate and sodium p-styrene sulfona ...
DOI:
10.1016/j.jcis.2014.06.044

78. Bone cement based nanohybrid as a super biomaterial for bone healing

Author:
Govinda Kapusetti; Nira Misra; Vakil Singh; Swati Srivastava; Partha Roy; Kausik Dana; Pralay Maiti
Source:
Journal of materials chemistry B 2014 v.2 no.25 pp. 3984-3997
ISSN:
2050-7518
Subject:
bioactive properties; biocompatibility; biocompatible materials; cell adhesion; cell viability; cement; chemical composition; fluorescence; heat production; histopathology; image analysis; iron; mixing; muscles; nanoclays; nanohybrids; necrosis; polymerization; polymethylmethacrylate; rabbits; radiography; silicates; spectroscopy; surgery; temperature; thermal stability
Abstract:
... A novel nanohybrid based on bone cement has been developed which is capable of healing fractured bone in 30 days, one-third of the time required for the natural healing process. Nanohybrids of bone cement based on poly(methyl methacrylate) (PMMA), currently used as a grouting material in joint replacement surgery, were prepared by simple mixing with organically modified layered silicates of varyin ...
DOI:
10.1039/c4tb00501e

79. Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles

Author:
Lingyan Cao; Jerome A. Werkmeister; Jing Wang; Veronica Glattauer; Keith M. McLean; Changsheng Liu
Source:
Biomaterials 2014 v.35 no.9 pp. 2730-2742
ISSN:
0142-9612
Subject:
adverse effects; alkaline phosphatase; biomechanics; bone formation; bone marrow; bone substitutes; cell adhesion; cell viability; chitosan; composite materials; encapsulation; energy-dispersive X-ray analysis; growth factors; hydrocolloids; image analysis; in vivo studies; mice; mineralization; models; nanoparticles; rabbits; resorption; scanning electron microscopy; tissue engineering; tomography; transmission electron microscopy
Abstract:
... Although rhBMP-2 has excellent ability to accelerate the repair of normal bone defects, limitations of its application exist in the high cost and potential side effects. This study aimed to develop a composite photopolymerisable hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles (PH/rhBMP-2/NPs) as the bone substitute to realize segmental bone defect repair at a low gro ...
DOI:
10.1016/j.biomaterials.2013.12.028

80. Bovine mammary epithelial cells retain stem-like phenotype in long-term cultures

Author:
Cravero Diego; Martignani Eugenio; Miretti Silvia; Macchi Elisabetta; Accornero Paolo; Baratta Mario
Source:
Research in veterinary science 2014 v.97 no.2 pp. 367-375
ISSN:
0034-5288
Subject:
adult stem cells; adults; cattle; cell adhesion; cell differentiation; epithelial cells; fibroblasts; lactation; mammary development; mammary glands; medicine; mice; milk proteins; phenotype; vimentin
Abstract:
... The detection and characterization of bovine mammary stem cells may give a better understanding of the cyclic characteristic of mammary gland development. In turn, this could potentially offer techniques to manipulate lactation yield and for regenerative medicine. We previously demonstrated that adult stem cells reside in the bovine mammary gland and possess an intrinsic regenerative potential. In ...
DOI:
10.1016/j.rvsc.2014.07.022

81. Btk29A Promotes Wnt4 Signaling in the Niche to Terminate Germ Cell Proliferation in Drosophila

Author:
Noriko Hamada-Kawaguchi; Beston F. Nore; Yusuke Kuwada; C. I. Edvard Smith; Daisuke Yamamoto
Source:
Science 2014 v.343 no.6168 pp. 294-297
ISSN:
0036-8075
Subject:
Drosophila; animal ovaries; carcinogenesis; cell adhesion; cell proliferation; germ cells; ovarian neoplasms; phosphorylation; somatic cells; transcription (genetics); tyrosine
Abstract:
... Wnt–β-Catenin in Germ Cells The Wnt–β-catenin pathway contributes to many signaling mechanisms during organismal development and carcinogenesis by regulating both transcription and cell adhesion. Hamada-Kawaguchi et al. (p. 294) demonstrate that this pathway must be activated in ovarian somatic cells to stop proliferation of germ cells in Drosophila. Phosphorylation of a tyrosine residue on β-cate ...
DOI:
10.1126/science.1244512

82. C-type natriuretic peptide attenuates LPS-induced endothelial activation: involvement of p38, Akt, and NF-κB pathways

Author:
Gan Chen; Jingxiang Zhao; Yujing Yin; Bo Wang; Qingjun Liu; Penglong Li; Lian Zhao; Hong Zhou
Source:
Amino acids 2014 v.46 no.12 pp. 2653-2663
ISSN:
0939-4451
Subject:
Western blotting; adhesion; cell adhesion; fluorescence; human umbilical vein endothelial cells; mitogen-activated protein kinase; natriuretic peptides; pathogenesis; phosphorylation; reactive oxygen species; reverse transcriptase polymerase chain reaction; sepsis (infection); signal transduction; transcription factor NF-kappa B; vascular diseases
Abstract:
... Endothelial activation elicited by inflammatory agents is regarded as a key event in the pathogenesis of several vascular inflammatory diseases. In the present study, the inhibitory effects and underlying mechanism of C-type natriuretic peptide (CNP) on LPS-induced endothelial activation were examined in human umbilical vein endothelial cells (HUVECs). The effect of CNP on adhesion molecule expres ...
DOI:
10.1007/s00726-014-1816-x

83. C/EBP-α, involvement of a novel transcription factor in leptin-induced VCAM-1 production in mouse chondrocytes

Author:
Xia Kang; Qing-Yun Xie; Jing-Song Zhou; Bo Zhang; Dong-Fa Liao; Hong-Hua Wu; Wei Zheng; Xian-Ming Pan; Yi Quan
Source:
FEBS letters 2014 v.588 no.7 pp. 1122-1127
ISSN:
0014-5793
Subject:
animal disease models; cell adhesion; chondrocytes; dose response; gene expression regulation; genes; leptin; messenger RNA; mice; obesity; osteoarthritis; transcription factors
Abstract:
... Leptin and vascular cell adhesion molecules-1 (VCAM-1) are two important mediators in obesity-related osteoarthritis, while the molecular mechanism linking leptin to VCAM-1 production is still obscure. Here we show that leptin upregulates VCAM-1 mRNA and protein levels in a time- and dose-dependent manner. Mechanistically, leptin induces VCAM-1 promoter activity by increasing the expression of C/E ...
DOI:
10.1016/j.febslet.2014.02.032

84. CD97 inhibits cell migration in human fibrosarcoma cells by modulating TIMP‐2/MT1‐ MMP/MMP‐2 activity – role of GPS autoproteolysis and functional cooperation between the N‐ and C‐terminal fragments

Author:
Cheng‐Chih Hsiao; Wen‐Chih Wang; Wan‐Lin Kuo; Hsin‐Yi Chen; Tse‐Ching Chen; Jörg Hamann; Hsi‐Hsien Lin
Source:
FEBS journal 2014 v.281 no.21 pp. 4878-4891
ISSN:
1742-464X
Subject:
G-protein coupled receptors; cell adhesion; cell movement; fibrosarcoma; gelatinase A; genes; global positioning systems; humans; integrins; interstitial collagenase; proteolysis; secretion
Abstract:
... CD97 is a tumor‐associated adhesion‐class G‐protein‐coupled receptor involved in modulating cell migration. Adhesion‐class G‐protein‐coupled receptors are characterized by proteolytic cleavage at a G‐protein‐coupled receptor proteolysis site (GPS) into an N‐terminal fragment (NTF) and a C‐terminal fragment (CTF), which remain associated noncovalently. The molecular mechanism and the role of GPS pr ...
DOI:
10.1111/febs.13027

85. Cadherin mutation linked to resistance to Cry1Ac affects male paternity and sperm competition in Helicoverpa armigera

Author:
Haonan Zhang; Bing Du; Yihua Yang; Dawn M. Higginson; Yves Carrière; Yidong Wu
Source:
Journal of insect physiology 2014 v.70 pp. 67-72
ISSN:
0022-1910
Subject:
Bacillus thuringiensis; Helicoverpa armigera; alleles; cadherins; cell adhesion; cotton; evolution; females; heterozygosity; homozygosity; insect physiology; life history; males; morphogenesis; mutation; paternity; pests; progeny; sperm competition; spermatozoa; transmembrane proteins
Abstract:
... Several lepidopteran pests of cotton have cadherin-based resistance to the Bacillus thuringiensis (Bt) toxin Cry1Ac. Cadherins are transmembrane proteins that mediate cell–cell adhesion and tissue morphogenesis, suggesting that fitness costs associated with cadherin mutations may be present in many aspects of life history. To evaluate whether cadherin-based resistance is associated with fitness co ...
DOI:
10.1016/j.jinsphys.2014.08.010
PubMed:
25220924
PubMed Central:
PMC4334375

86. Cannabinoid inhibits HIV-1 Tat-stimulated adhesion of human monocyte-like cells to extracellular matrix proteins

Author:
Erinn S. Raborn; Melissa Jamerson; Francine Marciano-Cabral; Guy A. Cabral
Source:
Life sciences 2014 v.104 no.1-2 pp. 15-23
ISSN:
0024-3205
Subject:
CD29 antigen; Human immunodeficiency virus 1; actin; adhesion; blood-brain barrier; cannabinoid receptors; cannabinoids; cell adhesion; extracellular matrix; gels; humans; laminin; monocytes; polymerization; therapeutics
Abstract:
... The aim of this study was to assess the effect of select cannabinoids on human immunodeficiency virus type 1 (HIV-1) transactivating (Tat) protein-enhanced monocyte-like cell adhesion to proteins of the extracellular matrix (ECM).Collagen IV, laminin, or an ECM gel was used to construct extracellular matrix layers. Human U937 monocyte-like cells were exposed to Tat in the presence of ∆⁹-tetrahydro ...
DOI:
10.1016/j.lfs.2014.04.008
PubMed:
24742657
PubMed Central:
PMC4089388

87. Carbonylated Plasma Proteins As Potential Biomarkers of Obesity Induced Type 2 Diabetes Mellitus

Author:
Bollineni Ravi Chand; Fedorova Maria; Blüher Matthias; Hoffmann Ralf
Source:
Journal of Proteome Research 2014 v.13 no.11 pp. 5081-5093
ISSN:
1535-3907
Subject:
affinity chromatography; angiogenesis; avidin; biomarkers; blood proteins; cell adhesion; cytoskeleton; diabetes mellitus; endothelium; humans; liver; moieties; obesity; oxidative stress; patients; peptides; post-translational modification; proteome; trypsin; vascular endothelial growth factor receptor-2
Abstract:
... Protein carbonylation is a common nonenzymatic oxidative post-translational modification, which is often considered as biomarker of oxidative stress. Recent evidence links protein carbonylation also to obesity and type 2 diabetes mellitus (T2DM), though the protein targets of carbonylation in human plasma have not been identified. In this study, we profiled carbonylated proteins in plasma samples ...
DOI:
10.1021/pr500324y

88. Carborane–β-cyclodextrin complexes as a supramolecular connector for bioactive surfaces

Author:
P. Neirynck; J. Schimer; P. Jonkheijm; L.-G. Milroy; P. Cigler; L. Brunsveld
Source:
Journal of materials chemistry B 2014 v.3 no.4 pp. 539-545
ISSN:
2050-7518
Subject:
beta-cyclodextrin; bioactive properties; cell adhesion; chemistry; epitopes; focal adhesions; peptides
Abstract:
... Supramolecular chemistry provides an attractive entry to generate dynamic and well-controlled bioactive surfaces. Novel host–guest systems are urgently needed to provide a broader affinity and applicability portfolio. A synthetic strategy to carborane–peptide bioconjugates was therefore developed to provide an entry to monovalent supramolecular functionalization of β-cyclodextrin coated surfaces. ...
DOI:
10.1039/c4tb01489h

89. Carboxybetaine Methacrylate-Modified Nylon Surface for Circulating Tumor Cell Capture

Author:
Wang Huiyu; Yue Guofeng; Dong Chaoqun; Wu Fenglei; Wei Jia; Yang Yang; Zou Zhengyun; Wang Lifeng; Qian Xiaoping; Zhang Tao; Liu Baorui
Source:
ACS Applied Materials & Interfaces 2014 v.6 no.6 pp. 4550-4559
ISSN:
1944-8252
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adhesion; antibodies; blood platelets; blood sampling; bovine serum albumin; cell adhesion; contact angle; cytotoxicity; mice; models; neoplasm cells; nylon
Abstract:
... Conventional in vitro circulating tumor cell (CTC) detection methods are always limited by blood sample volume because of the requirement of a large amount of blood. The aim of this study was to overcome the limitation by designing and making an in vivo CTC capture device. In this study, we designed and prepared a kind of proper material to serve the purpose of intervention. A method employing 3-a ...
DOI:
10.1021/am500394j

90. Carboxybetaine methacrylate oligomer modified nylon for circulating tumor cells capture

Author:
Chaoqun Dong; Huiyu Wang; Zhuo Zhang; Tao Zhang; Baorui Liu
Source:
Journal of colloid and interface science 2014 v.432 pp. 135-143
ISSN:
0021-9797
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adhesion; adsorption; antibodies; atomic force microscopy; biocompatibility; blood platelets; cell adhesion; contact angle; manufacturing; medical equipment; medicine; moieties; neoplasm cells; neoplasms; nylon; polymerization; proteins; zwitterions
Abstract:
... Circulating tumor cells (CTC) capture is one of the most effective approaches in diagnosis and treatment of cancers in the field of personalized cancer medicine. In our study, zwitterionic carboxybetaine methacrylate (CBMA) oligomers were grafted onto nylon via atomic transfer random polymerization (ATRP) which would serve as a novel material for the development of convenient CTC capture intervent ...
DOI:
10.1016/j.jcis.2014.07.003

91. Cardiac outflow tract development relies on the complex function of Sox4 and Sox11 in multiple cell types

Author:
Mandy H. Paul; Richard P. Harvey; Michael Wegner; Elisabeth Sock
Source:
Cellular and molecular life sciences 2014 v.71 no.15 pp. 2931-2945
ISSN:
1420-682X
Subject:
mechanism of action; neural crest; cell adhesion; transcription factors; humans; congenital abnormalities; cytoskeleton; mice; heart; extracellular matrix
Abstract:
... Congenital heart defects represent the most common human birth defects and are often life-threatening. Frequently, they are caused by abnormalities of the outflow tract whose formation results from coordinated development of cells from mesodermal and neural crest origin and depends on the activity of many different transcription factors. However, place, time, and mode of action have only been anal ...
DOI:
10.1007/s00018-013-1523-x

92. Catechol Chemistry Inspired Approach to Construct Self-Cross-Linked Polymer Nanolayers as Versatile Biointerfaces

Author:
Liu Xinyue; Deng Jie; Ma Lang; Cheng Chong; Nie Chuanxiong; He Chao; Zhao Changsheng
Source:
Langmuir 2014 v.30 no.49 pp. 14905-14915
ISSN:
1520-5827
Subject:
X-ray photoelectron spectroscopy; acrylic acid; adhesion; adsorption; anticoagulant activity; blood platelets; catechol; cell adhesion; cell proliferation; cell viability; crosslinking; dopamine; endothelial cells; fouling; hydrophilicity; pH; platelet activation; polymerization; polymers; surface roughness; thrombin; thromboplastin; wettability
Abstract:
... In this study, we proposed a catechol chemistry inspired approach to construct surface self-cross-linked polymer nanolayers for the design of versatile biointerfaces. Several representative biofunctional polymers, P(SS-co-AA), P(SBMA-co-AA), P(EGMA-co-AA), P(VP-co-AA), and P(MTAC-co-AA), were first synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization, and then the ...
DOI:
10.1021/la503872h

93. Celiac disease patient IgA antibodies induce endothelial adhesion and cell polarization defects via extracellular transglutaminase 2

Author:
Cristina Antonella Nadalutti; Ilma Rita Korponay-Szabo; Katri Kaukinen; Martin Griffin; Markku Mäki; Katri Lindfors
Source:
Cellular and molecular life sciences 2014 v.71 no.7 pp. 1315-1326
ISSN:
1420-682X
Subject:
CD29 antigen; adhesion; angiogenesis; apoptosis; autoantibodies; celiac disease; cell adhesion; cell movement; crosslinking; endothelial cells; enzyme activity; extracellular matrix; fibronectins; immunoglobulin A; patients; phenotype; protein-glutamine gamma-glutamyltransferase; secretion; signal transduction
Abstract:
... We have recently found that celiac disease patient serum-derived autoantibodies targeted against transglutaminase 2 interfere with several steps of angiogenesis, including endothelial sprouting and migration, though the mechanism involved remained to be fully characterized. This study now investigated the processes underlying the antiangiogenic effects exerted by celiac disease patient antibodies ...
DOI:
10.1007/s00018-013-1455-5

94. Cell adhesion and invasion inhibitory effect of an ovarian cancer targeting peptide selected via phage display in vivo

Author:
Ximing Pu; Chuying Ma; Guangfu Yin; Fei You; Yan Wei
Source:
Biochemical and biophysical research communications 2014 v.443 pp. 858-863
ISSN:
0006-291X
Subject:
animal ovaries; bacteriophages; cell adhesion; extracellular matrix; metastasis; neoplasm cells; ovarian neoplasms
Abstract:
... Organ-specific metastasis is of great importance since most of the cancer deaths are caused by spread of the primary cancer to distant sites. Therefore, targeted anti-metastases therapies are needed to prevent cancer cells from metastasizing to different organs. The phage clone pc3-1 displaying peptide WSGPGVWGASVK selected by phage display had been identified which have high binding efficiency an ...
DOI:
10.1016/j.bbrc.2013.12.058

95. Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein

Author:
Hsin-Hou Chang; Po-Kong Chen; Guan-Ling Lin; Chun-Jen Wang; Chih-Hsien Liao; Yu-Cheng Hsiao; Jing-Hua Dong; Der-Shan Sun
Source:
Journal of virological methods 2014 v.201 pp. 1-6
ISSN:
0166-0934
Subject:
Middle East respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus; adhesion inhibition assay; amino acids; binding sites; cell adhesion; mortality; pathogens; psychosocial factors; synthetic peptides; therapeutics
Abstract:
... Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is requ ...
DOI:
10.1016/j.jviromet.2014.01.022
PubMed:
24530430
PubMed Central:
PMC7113645

96. Cell adhesion mechanisms on laterally mobile polymer films

Author:
Andreas P. Kourouklis; Ronald V. Lerum; Harry Bermudez
Source:
Biomaterials 2014 v.35 no.17 pp. 4827-4834
ISSN:
0142-9612
Subject:
adhesion; biomimetics; cell adhesion; composite polymers; extracellular matrix; fibroblasts; films (materials); focal adhesions; hydrophobicity; integrins; ligands; quantitative analysis
Abstract:
... In contrast with the majority of substrates used to study cell adhesion, the natural extracellular matrix (ECM) is dynamic and remodeled over time. Here we use amphiphilic block copolymers to create self-assembled supported films with tunable lateral mobility. These films are intended to serve as partial mimics of the ECM in order to better understand cell adhesion responses, specifically in the c ...
DOI:
10.1016/j.biomaterials.2014.02.052

97. Cell adhesion on supported lipid bilayers functionalized with RGD peptides monitored by using a quartz crystal microbalance with dissipation

Author:
Xiaohua Zhu; Zhao Wang; Ansha Zhao; Nan Huang; Huadong Chen; Shuo Zhou; Xiao Xie
Source:
Colloids and Surfaces B: Biointerfaces 2014 v.116 pp. 459-464
ISSN:
0927-7765
Subject:
fluorescence; colloids; quartz; peptides; cytoskeleton; lipids; stem cells; adhesion; lipid bilayers; cell adhesion; adsorption
Abstract:
... Supported lipid bilayer (SLB) is one of the most widely used structures to mimic cell membranes. To study the cell–cell, cell–matrix and cell–material interactions, supported lipid bilayers (SLBs) functionalized with RGD peptides (SLBs–RGD) were prepared by vesicle fusion on a SiO2 quartz crystal, and subsequently bone mesenchymal stem cells (BMSCs) adhesion was analyzed. A quartz crystal microbal ...
DOI:
10.1016/j.colsurfb.2014.01.032

98. Cell fate regulation during preimplantation development: A view of adhesion-linked molecular interactions

Author:
Berna Sozen; Alp Can; Necdet Demir
Source:
Developmental biology 2014 v.395 no.1 pp. 73-83
ISSN:
0012-1606
Subject:
blastomeres; cell adhesion; cell polarity; gene regulatory networks; mammals; models; morphogenesis; transcription (genetics)
Abstract:
... In the developmental process of the early mammalian embryo, it is crucial to understand how the identical cells in the early embryo later develop different fates. Along with existing models, many recently discovered molecular, cellular and developmental factors play roles in cell position, cell polarity and transcriptional networks in cell fate regulation during preimplantation. A structuring proc ...
DOI:
10.1016/j.ydbio.2014.08.028

99. Cell surface N-glycans influence the level of functional E-cadherin at the cell–cell border

Author:
M. Kristen Hall; Douglas A. Weidner; Sahil Dayal; Ruth A. Schwalbe
Source:
FEBS Open Bio 2014 v.4 pp. 892-897
ISSN:
2211-5463
Subject:
cadherins; cell adhesion; cells; polysaccharides
Abstract:
... E-cadherin is crucial for adhesion of cells to each other and thereby development and maintenance of tissue. While it is has been established that N-glycans inside the cell impact the level of E-cadherin at the cell surface of epithelial-derived cells, it is unclear whether N-glycans outside the cell control the clustering of E-cadherin at the cell–cell border. Here, we demonstrate reduction of N- ...
DOI:
10.1016/j.fob.2014.10.006
PubMed:
25379387
PubMed Central:
PMC4215119

100. Characterisation and assessment of electrospun Poly/hydroxyapatite nanofibres together with a cell adhesive for bone repair applications

Author:
SY Heo; JW Seol; NS Kim
Source:
Veterinární medicína v.59 no.10 pp. 498-501
ISSN:
0375-8427
Subject:
adhesion; cell adhesion; extracellular matrix; gelatin; hydroxyapatite; nanofibers
Abstract:
... In this study, we fabricated Poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres using electrospinning and evaluated their potential use for bone repair applications. Analysis confirmed that the PLGA nanofibres were similar to the natural extracellular matrix and included HAp particles. Further, gelatin augmented the adhesion of electrospun nanofibres in the cell adhesion test. Therefo ...
DOI:
10.17221/7780-vetmed