Publication year rev: 7986-2014 / Subject term: extracellular matrix

1. (±)-Aspongamide A, an N-Acetyldopamine Trimer Isolated from the Insect Aspongopus chinensis, Is an Inhibitor of p-Smad3

Author:: Yan Yong-Ming; Ai Jun; Shi Yan-Ni; Zuo Zhi-Li; Hou Bo; Luo Jie; Cheng Yong-Xian
Source:: Organic letters 2014 v.16 no.2 pp. 532-535
ISSN:: 1523-7052
Subject:: Aspongopus; enantiomers; extracellular matrix; insects; organic compounds; phosphorylation; rats; spectroscopy; transforming growth factor beta 1
Abstract:: ... (±)-Aspongamide A (1), an unusual trimer of N-acetyldopamine (NADA) bearing a novel tetrahydrobenzo[a]dibenzo[b,e][1,4]dioxine structure, and a pair of NADA dimeric enantiomers (2) were isolated from Aspongopus chinensis. The structures of compounds 1 and 2 were assigned using spectroscopic methods. Compound 1 was found to be an inhibitor of Smad3 phosphorylation in transforming growth factor-β1 ( ...
DOI:: 10.1021/ol403409v
: https://doi.org/10.1021/ol403409v

2. 1H NMR spectroscopic studies establish that heparanase is a retaining glycosidase

Author:: Jennifer C. Wilson; Andrew Elohim Laloo; Sanjesh Singh; Vito Ferro
Source:: Biochemical and biophysical research communications 2014 v.443 pp. 185-188
ISSN:: 0006-291X
Subject:: angiogenesis; anti-inflammatory agents; extracellular matrix; glycosides; heparan sulfate; hydrolysis; inflammation; metastasis; mutagenicity; nuclear magnetic resonance spectroscopy; prediction; proteoglycans; sequence analysis; spectral analysis
Abstract:: ... Heparanase is an endo-β-glucuronidase that cleaves heparan sulfate side chains of proteoglycans in basement membranes and the extracellular matrix (ECM). Heparanase is implicated in several diverse pathological processes associated with ECM degradation such as metastasis, inflammation and angiogenesis and is thus an important target for anti-cancer and anti-inflammatory drug discovery. Heparanase ...
DOI:: 10.1016/j.bbrc.2013.11.079
: https://doi.org/10.1016/j.bbrc.2013.11.079

3. 2,5-Dimethoxy 2,5-dihydrofuran crosslinked chitosan fibers enhance bone regeneration in rabbit femur defects

Author:: Paulomi Ghosh; Arun Prabhu Rameshbabu; Nantu Dogra; Santanu Dhara
Source:: RSC advances 2014 v.4 no.37 pp. 19516-19524
ISSN:: 2046-2069
Subject:: adhesion; adsorption; alkaline phosphatase; animal models; bone formation; bone marrow; calcium; cell proliferation; chemical bonding; chitosan; crosslinking; extracellular matrix; femur; glucosamine; histology; humans; hydrophobicity; imines; in vivo studies; mesenchymal stromal cells; neutralization; osteocalcin; pH; polymers; rabbits; secretion; sodium hydroxide; tensile strength; tissue engineering
Abstract:: ... Chitosan fibers were fabricated via pH induced neutralization and precipitation in a 5 w/v% NaOH bath. Intermolecular covalent crosslinking of these fibers were performed through imine linkages between the glucosamine units of polymers and the dialdehyde groups of 2,5-dimethoxy-2,5-dihydrofuran at 60 °C, pH 2.2. The covalently crosslinked fibers demonstrated improved tensile strength, stiffness, h ...
DOI:: 10.1039/c4ra01971g
: https://doi.org/10.1039/c4ra01971g

4. 3D Tissue Engineered Supramolecular Hydrogels for Controlled Chondrogenesis of Human Mesenchymal Stem Cells

Author:: Jung Hyuntae; Park Ji Sun; Yeom Junseok; Selvapalam Narayanan; Park Kyeng Min; Oh Kyunghoon; Yang Jeong-A; Park Keun Hong; Hahn Sei Kwang; Kim Kimoon
Source:: Biomacromolecules 2014 v.15 no.3 pp. 707-714
ISSN:: 1526-4602
Subject:: cartilage; chondrogenesis; crosslinking; dexamethasone; extracellular matrix; glycosaminoglycans; humans; hydrogels; immunohistochemistry; mechanical properties; quantitative polymerase chain reaction; stem cells; tissue engineering; transforming growth factor beta 3
Abstract:: ... Despite a wide investigation of hydrogels as an artificial extracellular matrix, there are few scaffold systems for the facile spatiotemporal control of mesenchymal stem cells (MSCs). Here, we report 3D tissue engineered supramolecular hydrogels prepared with highly water-soluble monofunctionalized cucurbit[6]uril–hyaluronic acid (CB[6]-HA), diaminohexane conjugated HA (DAH-HA), and drug conjugate ...
DOI:: 10.1021/bm401123m
: https://doi.org/10.1021/bm401123m

5. A 3-D in vitro co-culture model of mammary gland involution

Author:: Jonathan J. Campbell; Laur-Alexandru Botos; Timothy J. Sargeant; Natalia Davidenko; Ruth E. Cameron; Christine J. Watson
Source:: Integrative biology 2014 v.6 no.6 pp. 618-626
ISSN:: 1757-9708
Subject:: adipose tissue; autophagy; breast neoplasms; breasts; cell lines; coculture; collagen; cytokines; drugs; epithelial cells; epithelium; extracellular matrix; high-throughput screening methods; hyaluronic acid; macrophages; mammary glands; models; monocytes; phenotype; progeny; prolactin; signal transduction; therapeutics; tissue repair; weaning
Abstract:: ... Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues of the breast cou ...
DOI:: 10.1039/c3ib40257f
: https://doi.org/10.1039/c3ib40257f

6. A 3D matrix platform for the rapid generation of therapeutic anti-human carcinoma monoclonal antibodies

Author:: David T. Dudley; Xiao-Yan Li; Casey Y. Hu; Celina G. Kleer; Amanda L. Willis; Stephen J. Weiss
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.41 pp. 14882-14887
ISSN:: 0027-8424
Subject:: antigens; antineoplastic activity; breast neoplasms; carcinoma; cell growth; collagen; humans; integrins; monoclonal antibodies; neoplasm cells; patients; tissues
Abstract:: ... Efforts to develop unbiased screens for identifying novel function-blocking monoclonal antibodies (mAbs) in human carcinomatous states have been hampered by the limited ability to design in vitro models that recapitulate tumor cell behavior in vivo. Given that only invasive carcinoma cells gain permanent access to type I collagen-rich interstitial tissues, an experimental platform was established ...
DOI:: 10.1073/pnas.1410996111
PubMed:: 25267635
PubMed Central:: PMC4205646
: https://doi.org/10.1073/pnas.1410996111

7. A Dictyostelium cellobiohydrolase orthologue that affects developmental timing

Author:: Mizuho Kunii; Mami Yasuno; Yuki Shindo; Takefumi Kawata
Source:: Development genes and evolution 2014 v.224 no.1 pp. 25-35
ISSN:: 0949-944X
Subject:: Dictyostelium discoideum; cellulose; cellulose 1,4-beta-cellobiosidase; culture media; endo-1,4-beta-glucanase; extracellular matrix; fruiting bodies; gel electrophoresis; genes; mutants; spores
Abstract:: ... Dictyostelium discoideum is a facultative multicellular amoebozoan with cellulose in the stalk and spore coat of its fruiting body as well as in the extracellular matrix of the migrating slug. The organism also harbors a number of cellulase genes. One of them, cbhA, was identified as a candidate cellobiohydrolase gene based on the strong homology of its predicted protein product to fungal cellobio ...
DOI:: 10.1007/s00427-013-0460-x
PubMed:: 24240571
: https://doi.org/10.1007/s00427-013-0460-x

8. A Nacre Protein, n16.3, Self-Assembles To Form Protein Oligomers That Dimensionally Limit and Organize Mineral Deposits

Author:: Perovic Iva; Chang Eric P.; Lui Michael; Rao Ashit; Cölfen Helmut; Evans John Spencer
Source:: Biochemistry 2014 v.53 no.16 pp. 2739-2748
ISSN:: 1520-4995
Subject:: Pinctada fucata; aragonite; calcite; calcium; calcium carbonate; extracellular matrix; gels; ions; mineralization; nacre; protein deficiencies; protein subunits; proteins; proteome; shell (molluscs)
Abstract:: ... The mollusk shell is a complex biological material that integrates mineral phases with organic macromolecular components such as proteins. The role of proteins in the formation of the nacre layer (aragonite mineral phase) is poorly understood, particularly with regard to the organization of mineral deposits within the protein extracellular matrix and the identification of which proteins are respon ...
DOI:: 10.1021/bi401721z
: https://doi.org/10.1021/bi401721z

9. A Nanostructured Synthetic Collagen Mimic for Hemostasis

Author:: Kumar Vivek A.; Taylor Nichole L.; Jalan Abhishek A.; Hwang Lyahn K.; Wang Benjamin K.; Hartgerink Jeffrey D.
Source:: Biomacromolecules 2014 v.15 no.4 pp. 1484-1490
ISSN:: 1526-4602
Subject:: amino acids; animals; biocompatible materials; biomimetics; blood coagulation; collagen; extracellular matrix; hemostasis; hydrogels; inflammation; interleukin-1beta; monocytes; nanofibers; secretion; thrombosis; transfection; tumor necrosis factor-alpha
Abstract:: ... Collagen is a major component of the extracellular matrix and plays a wide variety of important roles in blood clotting, healing, and tissue remodeling. Natural, animal derived, collagen is used in many clinical applications but concerns exist with respect to its role in inflammation, batch-to-batch variability, and possible disease transfection. Therefore, development of synthetic nanomaterials t ...
DOI:: 10.1021/bm500091e
PubMed:: 24694012
PubMed Central:: PMC3993945
: https://doi.org/10.1021/bm500091e

10. A Sporulation Factor Is Involved in the Morphological Change of Clostridium perfringens Biofilms in Response to Temperature

Author:: Nozomu Obana; Kouji Nakamura; Nobuhiko Nomura
Source:: Journal of bacteriology 2014 v.196 no.8 pp. 1540-1550
ISSN:: 0021-9193
Subject:: Clostridium perfringens; antibiotics; bacteria; bacteriology; biofilm; extracellular matrix; fimbriae; pathogenesis; pathogens; regulator genes; spores; sporulation; temperature
Abstract:: ... Biofilm formation has been associated with bacterial pathogenesis, such as nosocomial and chronic infections, as the resistance of biofilms to environmental stresses has increased. Clostridium perfringens is a Gram-positive spore-forming anaerobic pathogen. This organism survives antibiotic treatment through the formation of biofilms or spores, but the environmental and regulatory factors involved ...
DOI:: 10.1128/JB.01444-13
PubMed:: 24509316
PubMed Central:: PMC3993371
: https://doi.org/10.1128/JB.01444-13

11. A Study of Phenylalanine Side-Chain Dynamics in Surface-Adsorbed Peptides Using Solid-State Deuterium NMR and Rotamer Library Statistics

Author:: Li Kun; Emani Prashant S.; Ash Jason; Groves Michael; Drobny Gary P.
Source:: Journal of the American Chemical Society 2014 v.136 no.32 pp. 11402-11411
ISSN:: 1520-5126
Subject:: biofilm; biomineralization; deuterium; extracellular matrix; models; nuclear magnetic resonance spectroscopy; peptides; phenylalanine; proteins; stable isotopes; statistics
Abstract:: ... Extracellular matrix proteins adsorbed onto mineral surfaces exist in a unique environment where the structure and dynamics of the protein can be altered profoundly. To further elucidate how the mineral surface impacts molecular properties, we perform a comparative study of the dynamics of nonpolar side chains within the mineral-recognition domain of the biomineralization protein salivary statheri ...
DOI:: 10.1021/ja504677d
: https://doi.org/10.1021/ja504677d

12. A cell-assembled, spatially aligned extracellular matrix to promote directed tissue development

Author:: Shivani Singh; Stephen B. Bandini; Patrick E. Donnelly; Jeffrey Schwartz; Jean E. Schwarzbauer
Source:: Journal of materials chemistry B 2014 v.2 no.11 pp. 1449-1453
ISSN:: 2050-7518
Subject:: chemistry; extracellular matrix; fibroblasts; fibronectins; neurites; polymers
Abstract:: ... A nanometer thick, micron scale-patterned interface on a polymeric material directs fibroblast proliferation into a highly aligned, confluent cell monolayer. These cells assemble fibronectin extracellular matrix (ECM) fibrils that are aligned with the pattern, and matrix alignment on the synthetic polymer surface is maintained throughout a decellularization process. Biologic relevance of this ECM- ...
DOI:: 10.1039/c3tb21864c
PubMed:: 24707354
PubMed Central:: PMC3975264
: https://doi.org/10.1039/c3tb21864c

13. A chondromimetic microsphere for in situ spatially controlled chondrogenic differentiation of human mesenchymal stem cells

Author:: Sharon Ansboro; Jessica S. Hayes; Valerie Barron; Shane Browne; Linda Howard; Udo Greiser; Pierce Lalor; Fintan Shannon; Frank P. Barry; Abhay Pandit; J. Mary Murphy
Source:: Journal of controlled release 2014 v.179 pp. 42-51
ISSN:: 0168-3659
Subject:: cartilage; cell viability; chondrogenesis; collagen; extracellular matrix; gene expression; genes; humans; hyaluronic acid; microparticles; models; phenotype; proteoglycans; staining; stem cells; tissue engineering; transforming growth factor beta 3
Abstract:: ... Human mesenchymal stem cells (hMSCs) have been identified as a viable cell source for cartilage tissue engineering. However, to undergo chondrogenic differentiation hMSCs require growth factors, in particular members of the transforming growth factor beta (TGF-β) family. While in vitro differentiation is feasible through continuous supplementation of TGF-β3, mechanisms to control and drive hMSCs d ...
DOI:: 10.1016/j.jconrel.2014.01.023
: https://doi.org/10.1016/j.jconrel.2014.01.023

14. A discoidin domain receptor 1 knock-out mouse as a novel model for osteoarthritis of the temporomandibular joint

Author:: Boris Schminke; Hayat Muhammad; Christa Bode; Boguslawa Sadowski; Regina Gerter; Nikolaus Gersdorff; Ralf Bürgers; Efrat Monsonego-Ornan; Vicki Rosen; Nicolai Miosge
Source:: Cellular and molecular life sciences 2014 v.71 no.6 pp. 1081-1096
ISSN:: 1420-682X
Subject:: chondrocytes; chondrogenesis; collagen; extracellular matrix; mice; models; osteoarthritis; proteoglycans; tissue repair
Abstract:: ... Discoidin domain receptor 1 (DDR-1)-deficient mice exhibited a high incidence of osteoarthritis (OA) in the temporomandibular joint (TMJ) as early as 9 weeks of age. They showed typical histological signs of OA, including surface fissures, loss of proteoglycans, chondrocyte cluster formation, collagen type I upregulation, and atypical collagen fibril arrangements. Chondrocytes isolated from the TM ...
DOI:: 10.1007/s00018-013-1436-8
: https://doi.org/10.1007/s00018-013-1436-8

15. A genome‐wide association study for canine cryptorchidism in Siberian Huskies

Author:: X. Zhao; S. Onteru; M. Saatchi; D. Garrick; M. Rothschild
Source:: Journal of animal breeding and genetics 2014 v.131 no.3 pp. 202-209
ISSN:: 0931-2668
Subject:: computer software; cryptorchidism; dogs; extracellular matrix; gelatinase B; genes; genetic variance; haplotypes; population structure; screening; single nucleotide polymorphism; testes
Abstract:: ... Cryptorchidism is a condition whereby one or both testes fail to descend into the scrotal sac. Here, we performed a genome‐wide association study (GWAS) with both a case–control analysis using the GEMMA software accounting for population structure and a BayesB approach in the GenSel software applied to every 1 Mb window of SNPs or haplotypes. The haplotypes were constructed from a genealogical tre ...
DOI:: 10.1111/jbg.12064
PubMed:: 24268032
: https://doi.org/10.1111/jbg.12064

16. A modular approach to easily processable supramolecular bilayered scaffolds with tailorable properties

Author:: Björne B. Mollet; Marta Comellas-Aragonès; A. J. H. Spiering; Serge H. M. Söntjens; E. W. Meijer; Patricia Y. W. Dankers
Source:: Journal of materials chemistry B 2014 v.2 no.17 pp. 2483-2493
ISSN:: 2050-7518
Subject:: anisotropy; biocompatible materials; epithelial cells; extracellular matrix; hydrogen bonding; kidneys; lactones; medicine; moieties; peptides; polyethylene glycol; tissue engineering; tissues
Abstract:: ... Engineering of anisotropic tissues demands extracellular matrix (ECM) mimicking scaffolds with an asymmetric distribution of functionalities. We here describe a convenient, modular approach based on supramolecular building blocks to form electrospun bilayered scaffolds with tailorable properties. Polymers and peptides functionalized with hydrogen-bonding ureido-pyrimidinone (UPy) moieties can easi ...
DOI:: 10.1039/c3tb21516d
: https://doi.org/10.1039/c3tb21516d

17. A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

Author:: Sohail Ejaz; Khaleeq Anwar; Robina Taj; Muhammad Ashraf
Source:: Open life sciences 2014 v.9 no.3 pp. 298-308
ISSN:: 2391-5412
Subject:: Opuntia stricta var. dillenii; angiogenesis; blood vessels; chickens; chorioallantoic membrane; diabetic neuropathy; extracellular matrix; new drugs; phytochemicals
Abstract:: ... Angiogenesis is the formation of new blood vessels from the existing blood vessels and is involved in both physiological and pathological events. Pathological angiogenesis provokes in several important diseases as in inflammatory diseases, ischemic conditions, diabetic neuropathy and cancer. The discovery of new drugs from phyto-chemicals has a long history and anti-angiogenic agents from plant so ...
DOI:: 10.2478/s11535-013-0266-x
: https://doi.org/10.2478/s11535-013-0266-x

18. A novel motif in the NaTrxh N-terminus promotes its secretion, whereas the C-terminus participates in its interaction with S-RNase in vitro

Author:: Alejandra Ávila-Castañeda; Javier Andrés Juárez-Díaz; Rogelio Rodríguez-Sotres; Carlos E Bravo-Alberto; Claudia Patricia Ibarra-Sánchez; Alejandra Zavala-Castillo; Yuridia Cruz-Zamora; León P Martínez-Castilla; Judith Márquez-Guzmán; Felipe Cruz-García
Source:: BMC plant biology 2014 v.14 no.1 pp. 147
ISSN:: 1471-2229
Subject:: Golgi apparatus; Nicotiana; apoplast; cell walls; endoplasmic reticulum; extracellular matrix; gene expression regulation; hydrophobicity; onions; protein-protein interactions; proteins; ribonucleases; secretion; signal peptide
Abstract:: ... BACKGROUND: NaTrxh, a thioredoxin type h, shows differential expression between self-incompatible and self-compatible Nicotiana species. NaTrxh interacts in vitro with S-RNase and co-localizes with it in the extracellular matrix of the stylar transmitting tissue. NaTrxh contains N- and C-terminal extensions, a feature shared by thioredoxin h proteins of subgroup 2. To ascertain the function of the ...
DOI:: 10.1186/1471-2229-14-147
PubMed:: 24886483
PubMed Central:: PMC4065587
: https://doi.org/10.1186/1471-2229-14-147

19. A robust pH-sensitive drug carrier: Aqueous micelles mineralized by calcium phosphate based on chitosan

Author:: Yan Lv; Hailong Huang; Bohan Yang; Huan Liu; Yapeng Li; Jingyuan Wang
Source:: Carbohydrate polymers 2014 v.111 pp. 101-107
ISSN:: 0144-8617
Subject:: blood serum; calcium chloride; calcium phosphates; chitosan; composite polymers; drugs; extracellular matrix; micelles; mineralization; nanocarriers; neoplasms; pH; sodium phosphate
Abstract:: ... This study developed an exciting alternative to the polymeric delivery system in cancer therapy. In this study, novel organic–inorganic hybrid polymeric micelles based on chitosan were synthesized and found to be robust nanocarriers for intracellular controlled release drug delivery. The polymeric micelles of carboxymethyl chitosan-grafted-p(ethylene glycol)-dodecylamine (CMC-g-PEG-DDA) were readi ...
DOI:: 10.1016/j.carbpol.2014.04.082
: https://doi.org/10.1016/j.carbpol.2014.04.082

20. A role for carbohydrate recognition in mammalian sperm-egg binding

Author:: Gary F. Clark
Source:: Biochemical and biophysical research communications 2014 v.450 pp. 1195-1203
ISSN:: 0006-291X
Subject:: adhesion; binding proteins; blastocyst; coatings; eggs; embryogenesis; exocytosis; extracellular matrix; hatching; humans; immune response; lectins; ligands; lymphocytes; mice; models; plasma membrane; spermatozoa; swine; zona pellucida
Abstract:: ... Mammalian fertilization usually requires three sequential cell–cell interactions: (i) initial binding of sperm to the specialized extracellular matrix coating the egg known as the zona pellucida (ZP); (ii) binding of sperm to the ZP via the inner acrosomal membrane that is exposed following the induction of acrosomal exocytosis; and (iii) adhesion of acrosome-reacted sperm to the plasma membrane o ...
DOI:: 10.1016/j.bbrc.2014.06.051
: https://doi.org/10.1016/j.bbrc.2014.06.051

21. A shape-controlled tuneable microgel platform to modulate angiogenic paracrine responses in stem cells

Author:: Dilip Thomas; Gianluca Fontana; Xizhe Chen; Clara Sanz-Nogués; Dimitrios I. Zeugolis; Peter Dockery; Timothy O'Brien; Abhay Pandit
Source:: Biomaterials 2014 v.35 pp. 8757-8766
ISSN:: 0142-9612
Subject:: animal models; cell culture; extracellular matrix; humans; ischemia; proteins; stem cells
Abstract:: ... Development of cell delivery platforms have been driven based on an empirical cytoprotective design. While cell-matrix and cell–cell interactions that influence biochemical effects beyond survival has been limited and overshadowed in an effort to incrementally improve biomimicking properties of the tissue-engineered constructs. Here we demonstrate fabrication of a shape controlled 3D type-I collag ...
DOI:: 10.1016/j.biomaterials.2014.06.053
: https://doi.org/10.1016/j.biomaterials.2014.06.053

22. A spectrophotometer-based diffusivity assay reveals that diffusion hindrance of small molecules in extracellular matrix gels used in 3D cultures is dominated by viscous effects

Author:: Roland Galgoczy; Isabel Pastor; Adai Colom; Alicia Giménez; Francesc Mas; Jordi Alcaraz
Source:: Colloids and Surfaces B: Biointerfaces 2014 v.120 pp. 200-207
ISSN:: 0927-7765
Subject:: brain; collagen; colloids; cost effectiveness; diffusivity; equations; extracellular matrix; fibrin; gels; molecular weight; porosity; spectrophotometers; tissues; viscosity
Abstract:: ... The design of 3D culture studies remains challenging due to the limited understanding of extracellular matrix (ECM)-dependent hindered diffusion and the lack of simple diffusivity assays. To address these limitations, we set up a cost-effective diffusivity assay based on a Transwell plate and the spectrophotometer of a Microplate Reader, which are readily accessible to cell biology groups. The spe ...
DOI:: 10.1016/j.colsurfb.2014.05.017
: https://doi.org/10.1016/j.colsurfb.2014.05.017

23. Absence of feedback regulation in the synthesis of COL1A1

Author:: Kevin Dzobo; Virna D. Leaner; M. Iqbal Parker
Source:: Life sciences 2014 v.103 no.1 pp. 25-33
ISSN:: 0024-3205
Subject:: Western blotting; cell growth; collagen; extracellular matrix; fibroblasts; gene expression; messenger RNA; metalloproteinases; polypeptides; protein content; quantitative polymerase chain reaction; transforming growth factor beta
Abstract:: ... Recent studies have emphasized the importance of the extracellular microenvironment in modulating cell growth, motility, and signalling. In this study we have evaluated the ability of a fibroblast derived-extracellular matrix (fd-ECM) to regulate type I collagen synthesis and degradation in fibroblasts.Fibroblasts were plated on plastic (control) or on fd-ECM and type I collagen synthesis and degr ...
DOI:: 10.1016/j.lfs.2014.03.006
: https://doi.org/10.1016/j.lfs.2014.03.006

24. Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin

Author:: Yasuo Hitsumoto; Naomi Morita; Ryosuke Yamazoe; Mika Tagomori; Tsutomu Yamasaki; Seiichi Katayama
Source:: Anaerobe 2014 v.25 pp. 67-71
ISSN:: 1075-9964
Subject:: Clostridium perfringens; bacterial adhesion; collagen; dose response; extracellular matrix; fibronectins; gelatin
Abstract:: ... The adhesive properties of Clostridium perfringens to collagens, gelatin, fibronectin (Fn), Fn-prebound collagens, and Fn-prebound gelatin were investigated. C. perfringens could bind to Fn-prebound collagen type II, type III, and gelatin, but not to gelatin or collagens except for collagen type I directly. Recombinant Fn-binding proteins of C. perfringens, rFbpA and rFbpB, were used to examine Fn ...
DOI:: 10.1016/j.anaerobe.2013.11.002
: https://doi.org/10.1016/j.anaerobe.2013.11.002

25. Age-related disruption of autophagy in dermal fibroblasts modulates extracellular matrix components

Author:: Kanae Tashiro; Mayumi Shishido; Keiko Fujimoto; Yuko Hirota; Kazuyuki Yo; Takamasa Gomi; Yoshitaka Tanaka
Source:: Biochemical and biophysical research communications 2014 v.443 pp. 167-172
ISSN:: 0006-291X
Subject:: Western blotting; autophagy; collagen; elastin; extracellular matrix; fibroblasts; fluorescence microscopy; humans; hyaluronic acid; proteinase inhibitors; transmission electron microscopy; vacuoles; women
Abstract:: ... Autophagy is an intracellular degradative system that is believed to be involved in the aging process. The contribution of autophagy to age-related changes in the human skin is unclear. In this study, we examined the relationship between autophagy and skin aging. Transmission electron microscopy and immunofluorescence microscopy analyses of skin tissue and cultured dermal fibroblasts derived from ...
DOI:: 10.1016/j.bbrc.2013.11.066
: https://doi.org/10.1016/j.bbrc.2013.11.066

26. Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent

Author:: Qing Li; Xin Liu; Jin Wei
Source:: Biochemical and biophysical research communications 2014 v.452 pp. 497-502
ISSN:: 0006-291X
Subject:: blood serum; cardiomyocytes; extracellular matrix; fibroblasts; fibrosis; humans; interleukin-4; interleukin-6; rats
Abstract:: ... Periostin, as an extracellular matrix (ECM) protein, plays a critical role in myocardial fibrosis and also might be involved in the heart inflammatory process since it is a downstream molecule of IL4 and IL13. Considering the possible important role of periostin in heart aging, this study explored periostin expression pattern in both rat and human, the effect of periostin expression on cardiomyocy ...
DOI:: 10.1016/j.bbrc.2014.08.109
: https://doi.org/10.1016/j.bbrc.2014.08.109

27. Aggrecan is required for growth plate cytoarchitecture and differentiation

Author:: Kristen L. Lauing; Mauricio Cortes; Miriam S. Domowicz; Judith G. Henry; Alexis T. Baria; Nancy B. Schwartz
Source:: Developmental biology 2014 v.396 no.2 pp. 224-236
ISSN:: 0012-1606
Subject:: cartilage; cell death; chicks; chondrocytes; deformation; embryo (animal); extracellular matrix; fibroblast growth factor receptor 3; gene expression; genes; growth factors; growth plate; homozygosity; messenger RNA; mice; models; mortality; mutants; mutation; phenotype; proteoglycans; skeletal development; transgenic animals; vertebrae
Abstract:: ... The proteoglycan aggrecan is a prominent component of the extracellular matrix in growth plate cartilage. A naturally occurring, recessive, perinatally lethal mutation in the aggrecan core protein gene, cmdᵇᶜ (Acanᶜᵐᵈ⁻ᴮᶜ), that deletes the entire protein-coding sequence provided a model in which to characterize the phenotypic and morphologic effects of aggrecan deletion on skeletal development. We ...
DOI:: 10.1016/j.ydbio.2014.10.005
PubMed:: 25446537
PubMed Central:: PMC4261049
: https://doi.org/10.1016/j.ydbio.2014.10.005

28. Akt induces down regulation of MUC5AC production in NCI-H292 human airway epithelial cells cultured on extracellular matrix

Author:: Jun Iwashita; Yuho Ito; Mari Yokoo; Sunao Takahashi; Jun Murata
Source:: Bioscience, biotechnology, and biochemistry 2014 v.78 no.2 pp. 212-221
ISSN:: 1347-6947
Subject:: asthma; biotechnology; collagen; epithelium; extracellular matrix; humans; integrins; laminin; mucins; signal transduction
Abstract:: ... MUC5AC mucin overproduction is a key feature of asthma as contributes to airway obstruction. The production of MUC5AC is regulated in part by signals from extracellular matrix via integrin pathways, but it remains largely unclear. We investigated the role of Akt, a typical signal transducer in the integrin pathway, in the regulation of MUC5AC production. When NCI-H292 human airway epithelial cells ...
DOI:: 10.1080/09168451.2014.877829
: https://doi.org/10.1080/09168451.2014.877829

29. Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gels

Author:: Eric J. Berns; Shantanu Sur; Liuliu Pan; Joshua E. Goldberger; Sunitha Suresh; Shuming Zhang; John A. Kessler; Samuel I. Stupp
Source:: Biomaterials 2014 v.35 no.1 pp. 185-195
ISSN:: 0142-9612
Subject:: cell growth; cell movement; encapsulation; epitopes; extracellular matrix; gels; nanofibers; neurites; regrowth; spinal cord; stem cells; tissue repair; tissues
Abstract:: ... Regeneration of neural tissues will require regrowth of axons lost due to trauma or degeneration to reestablish neuronal connectivity. One approach toward this goal is to provide directional cues to neurons that can promote and guide neurite growth. Our laboratory previously reported the formation of aligned monodomain gels of peptide amphiphile (PA) nanofibers over macroscopic length scales. In t ...
DOI:: 10.1016/j.biomaterials.2013.09.077
PubMed:: 24120048
PubMed Central:: PMC3865987
: https://doi.org/10.1016/j.biomaterials.2013.09.077

30. Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles

Author:: Akira C. Saito; Tsubasa S. Matsui; Masaaki Sato; Shinji Deguchi
Source:: Biotechnology letters 2014 v.36 no.2 pp. 391-396
ISSN:: 0141-5492
Subject:: adhesion; deformation; equipment; extracellular matrix; medicine; tissue repair
Abstract:: ... Sheets of cells can be used for tissue regenerative medicine. Cell alignment within the sheet is now a key factor in the next generation of this technology. Anisotropic cell sheets without random cell orientations have been conventionally produced with photolithographically, microfabricated substrates using special facilities and equipment. Here we demonstrate a more accessible approach to the fab ...
DOI:: 10.1007/s10529-013-1368-2
PubMed:: 24101251
: https://doi.org/10.1007/s10529-013-1368-2

31. Alteration of energy metabolism gene expression in cumulus cells affects oocyte maturation via MOS–mitogen-activated protein kinase pathway in dairy cows with an unfavorable “Fertil−” haplotype of one female fertility quantitative trait locus

Author:: Daphné Brisard; Alice Desmarchais; Jean-Luc Touzé; Lionel Lardic; Sandrine Freret; Sebastien Elis; Fabienne Nuttinck; Sylvaine Camous; Joelle Dupont; Svetlana Uzbekova
Source:: Theriogenology 2014 v.81 no.4 pp. 599-612
ISSN:: 0093-691X
Subject:: dairy cows; embryogenesis; energy metabolism; extracellular matrix; fatty-acid synthase; female fertility; gene expression; gene expression regulation; genes; haplotypes; heifers; lipid metabolism; mitogen-activated protein kinase; oocytes; pregnancy; prostaglandin synthase; quantitative polymerase chain reaction; quantitative trait loci; reverse transcription
Abstract:: ... Prim'Holstein heifers selected for the “Fertil−” homozygous haplotype of QTL-Female-Fert ility-BTA3 showed a greater rate of early pregnancy failure and slower embryo development after IVM suggesting lower oocyte quality than those selected for “Fertile+”. We aimed to ascertain intrafollicular factors related to lower oocyte quality in “Fertil−” cows. Analysis of individual oocytes showed meiotic ...
DOI:: 10.1016/j.theriogenology.2013.11.013
: https://doi.org/10.1016/j.theriogenology.2013.11.013

32. Amelioration of diabetic nephropathy by orange peel extract in rats

Author:: Nishad Parkar; Veeranjaneyulu Addepalli
Source:: Natural product research 2014 v.28 no.23 pp. 2178-2181
ISSN:: 1478-6427
Subject:: blood; creatinine; diabetic nephropathy; fibrosis; kidneys; orange peels; rats; urea; urea nitrogen
Abstract:: ... This study was aimed to evaluate the effect of alcoholic orange peel extract (OPE) on streptozotocin-induced diabetic nephropathy. Four weeks after the induction of diabetes, treatment with OPE (100 and 200 mg/kg) was further given for 4 weeks. Treatment with OPE 200 improved renal functions and significantly prevented the increase in creatinine, urea and blood urea nitrogen levels. Histological a ...
DOI:: 10.1080/14786419.2014.925894
: https://doi.org/10.1080/14786419.2014.925894

33. Amphiphilic beads as depots for sustained drug release integrated into fibrillar scaffolds

Author:: Akhilesh K. Gaharwar; Silvia M. Mihaila; Ashish A. Kulkarni; Alpesh Patel; Andrea Di Luca; Rui L. Reis; Manuela E. Gomes; Clemens van Blitterswijk; Lorenzo Moroni; Ali Khademhosseini
Source:: Journal of controlled release 2014 v.187 pp. 66-73
ISSN:: 0168-3659
Subject:: alkaline phosphatase; bone formation; cell adhesion; composite polymers; dexamethasone; encapsulation; extracellular matrix; humans; hydrophobicity; mineralization; models; stem cells; tissue engineering; tissue repair
Abstract:: ... Native extracellular matrix (ECM) is a complex fibrous structure loaded with bioactive cues that affects the surrounding cells. A promising strategy to mimicking native tissue architecture for tissue engineering applications is to engineer fibrous scaffolds using electrospinning. By loading appropriate bioactive cues within these fibrous scaffolds, various cellular functions such as cell adhesion, ...
DOI:: 10.1016/j.jconrel.2014.04.035
PubMed:: 24794894
PubMed Central:: PMC4079754
: https://doi.org/10.1016/j.jconrel.2014.04.035

34. An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches

Author:: Shang-Jun Yin; Daeui Park; Yue-Xiu Si; Hae Young Chung; Yong-Doo Park; Jun-Mo Yang
Source:: Process Biochemistry 2014 v.49 pp. 1612-1621
ISSN:: 1359-5113
Subject:: proteins; RNA interference; protein synthesis; neoplasms; extracellular matrix; bioinformatics; data collection; DNA microarrays; acetaldehyde dehydrogenase; genes; atopic dermatitis; keratinocytes; algorithms; chemokines; small interfering RNA
Abstract:: ... The role of acetaldehyde dehydrogenase 1 (ALDH1) has been probed in several diseases, especially in various types of cancers. However, the role of ALDH1 in skin cells has not been well elucidated. In this study, we aimed to identify critical factors associated with ALDH1 in keratinocytes through gene and protein expression profiling approaches. To this end, we conducted serial OMICS studies, inclu ...
DOI:: 10.1016/j.procbio.2014.06.017
: https://doi.org/10.1016/j.procbio.2014.06.017

35. An ectopic approach for engineering a vascularized tracheal substitute

Author:: Chung-Kan Tsao; Chao-Yin Ko; Shu-Rui Yang; Chin-Yu Yang; Eric M. Brey; Simon Huang; I-Ming Chu; Ming-Huei Cheng
Source:: Biomaterials 2014 v.35 no.4 pp. 1163-1175
ISSN:: 0142-9612
Subject:: bone marrow; cartilage; chondrocytes; chondrogenesis; coculture; extracellular matrix; histology; mechanical properties; muscles; rabbits; sowing; stem cells; tissue engineering
Abstract:: ... Tissue engineering can provide alternatives to current methods for tracheal reconstruction. Here we describe an approach for ectopic engineering of vascularized trachea based on the implantation of co-cultured scaffolds surrounded by a muscle flap. Poly(L-lactic-co-glycolic acid) (PLGA) or poly(ε-caprolactone) (PCL) scaffolds were seeded with chondrocytes, bone marrow stem cells and co-cultured bo ...
DOI:: 10.1016/j.biomaterials.2013.10.055
: https://doi.org/10.1016/j.biomaterials.2013.10.055

36. Angiogenesis in Bone Regeneration: Tailored Calcium Release in Hybrid Fibrous Scaffolds

Author:: Castaño Oscar; Sachot Nadège; Xuriguera Elena; Engel Elisabeth; Planell Josep A.; Park Jeong-Hui; Jin Guang-Zhen; Kim Tae-Hyun; Kim Joong-Hyun; Kim Hae-Won
Source:: ACS applied materials 2014 v.6 no.10 pp. 7512-7522
ISSN:: 1944-8252
Subject:: angiogenesis; blood vessels; calcium; calcium phosphates; extracellular matrix; ions; scanning electron microscopy; silica; silicon; titanium dioxide; zeta potential
Abstract:: ... In bone regeneration, silicon-based calcium phosphate glasses (Bioglasses) have been widely used since the 1970s. However, they dissolve very slowly because of their high amount of Si (SiO₂ > 45%). Recently, our group has found that calcium ions released by the degradation of glasses in which the job of silicon is done by just 5% of TiO₂ are effective angiogenic promoters, because of their stimula ...
DOI:: 10.1021/am500885v
: https://doi.org/10.1021/am500885v

37. AngiotensinII induces HuR shuttling by post-transcriptional regulated CyclinD1 in human mesangial cells

Author:: Yu Che; Liu Yi; Javed Akhtar; Chen Bing; Zhang Ruiyu; Wan Qiang; Wang Rong
Source:: Molecular biology reports 2014 v.41 no.2 pp. 1141-1150
ISSN:: 0301-4851
Subject:: RNA interference; cell proliferation; extracellular matrix; humans; in vitro studies; kidney diseases; kidneys; messenger RNA; sclerosis; translation (genetics)
Abstract:: ... Abnormal proliferation of human mesangial cells was the earliest pathological character in chronic kidney disease and linked to the accumulation of extracellular matrix and glomerular sclerosis. Multifunctional Angiotensin (AngII) had been emerged as a key player in initiation and progression of fibrogenic processes in kidney. In mesangial cells, treatment with the proliferation stimulus AngII tri ...
DOI:: 10.1007/s11033-013-2960-1
: https://doi.org/10.1007/s11033-013-2960-1

38. Anti-fibrotic effect of thymoquinone on hepatic stellate cells

Author:: Mohammed Ghazwani; Yifei Zhang; Xiang Gao; Jie Fan; Jiang Li; Song Li
Source:: Phytomedicine 2014 v.21 pp. 254-260
ISSN:: 0944-7113
Subject:: animal models; extracellular matrix; gene expression; gene expression regulation; genes; humans; in vitro studies; in vivo studies; inflammation; interleukin-6; liver; liver cirrhosis; mechanism of action; messenger RNA; mice; protective effect; rats; transcription factor NF-kappa B
Abstract:: ... Hepatic stellate cells (HSCs) are the major cell type involved in the production of extracellular matrix in liver. After liver injury, HSCs undergo transdifferentiation process from quiescent state to activated state, which plays an important role in liver fibrosis. Previous studies have shown that thymoquinone (TQ) might have protective effect against liver fibrosis in animal models; however, the ...
DOI:: 10.1016/j.phymed.2013.09.014
: https://doi.org/10.1016/j.phymed.2013.09.014

39. Anti-inflammatory and Chondroprotective Activity of (+)-α-Pinene: Structural and Enantiomeric Selectivity

Author:: Rufino Ana T.; Ribeiro Madalena; Judas Fernando; Salgueiro Lígia; Lopes Maria C.; Cavaleiro Carlos; Mendes Alexandrina F.
Source:: Journal of natural products 2014 v.77 no.2 pp. 264-269
ISSN:: 1520-6025
Subject:: alpha-pinene; anti-inflammatory activity; beta-pinene; chondrocytes; cytotoxicity; drugs; extracellular matrix; genes; humans; inducible nitric oxide synthase; inflammation; metabolites; mitogen-activated protein kinase; physicochemical properties; transcription factor NF-kappa B
Abstract:: ... Previous studies have suggested that α-pinene, a common volatile plant metabolite, may have anti-inflammatory effects in human chondrocytes, thus exhibiting potential antiosteoarthritic activity. The objective of this study was to further characterize the potential antiosteoarthritic activity of selected pinene derivatives by evaluating their ability to modulate inflammation and extracellular matr ...
DOI:: 10.1021/np400828x
: https://doi.org/10.1021/np400828x

40. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice

Author:: A-Rang Im; Jae Hyoung Song; Mi Young Lee; Sung Hum Yeon; Key An Um; Sungwook Chae
Source:: BMC complementary and alternative medicine 2014 v.14 no.1 pp. 424
ISSN:: 1472-6882
Subject:: Cyclopia intermedia; alternative medicine; collagen; extracellular matrix; herbal tea; in vivo studies; leaves; mechanical properties; mice; photoaging; stems; ultraviolet radiation; South Africa
Abstract:: ... BACKGROUND: The fermented leaves and stems of Cyclopia intermedia are used to brew honeybush tea, an herbal tea indigenous to South Africa with reported anti-wrinkle effects. Wrinkle formation caused by photoaging clearly involves changes in extracellular matrix components and mechanical properties of the skin. METHODS: The inhibitory effects of honeybush extract and fermented honeybush on wrinkle ...
DOI:: 10.1186/1472-6882-14-424
PubMed:: 25355549
PubMed Central:: PMC4232710
: https://doi.org/10.1186/1472-6882-14-424

41. Antibiotic resistance in Pseudomonas aeruginosa biofilms: Towards the development of novel anti-biofilm therapies

Author:: Patrick K. Taylor; Amy T.Y. Yeung; Robert E.W. Hancock
Source:: Journal of biotechnology 2014 v.191 pp. 121-130
ISSN:: 0168-1656
Subject:: Pseudomonas aeruginosa; antibiotic resistance; antibiotics; bacteria; biofilm; environmental factors; extracellular matrix; health services; hospitals; nutrient availability; quorum sensing; resistance mechanisms
Abstract:: ... The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilms are recalcitrant to antibiotic therapies and immune clearance. This is particul ...
DOI:: 10.1016/j.jbiotec.2014.09.003
: https://doi.org/10.1016/j.jbiotec.2014.09.003

42. Association of prohibitin-1 and 2 with oxidative stress in rats with renal interstitial fibrosis

Author:: Tian-Biao Zhou; Yuan-Han Qin; Feng-Ying Lei; Wei-Fang Huang; Gregor P. C. Drummen
Source:: Molecular biology reports 2014 v.41 no.5 pp. 3033-3043
ISSN:: 0301-4851
Subject:: correlation; protein folding; anaerobic conditions; glutathione; fibrosis; oxidative stress; malondialdehyde; proteins; extracellular matrix; reactive oxygen species; superoxide dismutase; animal models; transforming growth factor beta 1; rats; antioxidant activity; apoptosis
Abstract:: ... Prohibitins PHB1 and PHB2 are evolutionary conserved and pleiotropic proteins, which have been shown to be important factors in various cellular functions, including proliferation, tumour suppression, apoptosis, transcription, and mitochondrial protein folding. Recently, we demonstrated that down-regulation promoted renal interstitial fibrosis (RIF) in ureteral obstructed rats. Furthermore, the hy ...
DOI:: 10.1007/s11033-014-3162-1
PubMed:: 24595445
: https://doi.org/10.1007/s11033-014-3162-1

43. Association of the SNP rs2623047 in the HSPG modification enzyme SULF1 with an Australian Caucasian Breast Cancer Cohort

Author:: Rachel K. Okolicsanyi; Marion Faure; Jose M.E. Jacinto; Diego Chacon-Cortes; Suzanne Chambers; Philippa H. Youl; Larisa M. Haupt; Lyn R. Griffiths
Source:: Gene 2014 v.547 pp. 50-54
ISSN:: 0378-1119
Subject:: alleles; breast neoplasms; cell proliferation; death; developed countries; disease detection; early diagnosis; enzymes; extracellular matrix; genotype; growth factors; heparan sulfate; ligands; patients; prognosis; proteoglycans; receptors; risk; single nucleotide polymorphism; survival rate; women
Abstract:: ... Breast cancer is the second most common cancer worldwide and the most common cancer reported in women. This malignant tumour is characterised by a number of specific features including uncontrolled cell proliferation. It ranks fifth in the world as a cause of cancer death overall in developed countries and is the second most frequent cause of cancer death in women. Early diagnosis increases 5-year ...
DOI:: 10.1016/j.gene.2014.06.009
: https://doi.org/10.1016/j.gene.2014.06.009

44. Attapulgite-doped electrospun poly(lactic-co-glycolic acid) nanofibers enable enhanced osteogenic differentiation of human mesenchymal stem cells

Author:: Zhe Wang; Yili Zhao; Yu Luo; Shige Wang; Mingwu Shen; Helena Tomás; Meifang Zhu; Xiangyang Shi
Source:: RSC advances 2014 v.5 no.4 pp. 2383-2391
ISSN:: 2046-2069
Subject:: alkaline phosphatase; biocompatibility; bone formation; calcium; clay; culture media; dexamethasone; enzyme activity; extracellular matrix; humans; hydrophilicity; medicine; mesenchymal stromal cells; nanofibers; nanorods; osteocalcin; polymers; secretion; staining; tissue engineering
Abstract:: ... The extracellular matrix mimicking property of electrospun polymer nanofibers affords their uses as an ideal scaffold material for differentiation of human mesenchymal stem cells (hMSCs), which is important for various tissue engineering applications. Here, we report the fabrication of electrospun poly(lactic-co-glycolic acid) (PLGA) nanofibers incorporated with attapulgite (ATT) nanorods, a clay ...
DOI:: 10.1039/c4ra09839k
: https://doi.org/10.1039/c4ra09839k

45. Augmentation of integrin-mediated mechanotransduction by hyaluronic acid

Author:: Anant Chopra; Maria E. Murray; Fitzroy J. Byfield; Melissa G. Mendez; Ran Halleluyan; David J. Restle; Dikla Raz-Ben Aroush; Peter A. Galie; Katarzyna Pogoda; Robert Bucki; Cezary Marcinkiewicz; Glenn D. Prestwich; Thomas I. Zarembinski; Christopher S. Chen; Ellen Puré; J. Yasha Kresh; Paul A. Janmey
Source:: Biomaterials 2014 v.35 no.1 pp. 71-82
ISSN:: 0142-9612
Subject:: actin; cadherins; cell movement; extracellular matrix; focal adhesions; gene expression regulation; homeostasis; hyaluronic acid; integrins; ligands; mechanotransduction; neoplasms; phenotype; receptors; transcription factors
Abstract:: ... Changes in tissue and organ stiffness occur during development and are frequently symptoms of disease. Many cell types respond to the stiffness of substrates and neighboring cells in vitro and most cell types increase adherent area on stiffer substrates that are coated with ligands for integrins or cadherins. In vivo cells engage their extracellular matrix (ECM) by multiple mechanosensitive adhesi ...
DOI:: 10.1016/j.biomaterials.2013.09.066
PubMed:: 24120037
PubMed Central:: PMC3930571
: https://doi.org/10.1016/j.biomaterials.2013.09.066

46. Avidin as a model for charge driven transport into cartilage and drug delivery for treating early stage post-traumatic osteoarthritis

Author:: Ambika G. Bajpayee; Cliff R. Wong; Moungi G. Bawendi; Eliot H. Frank; Alan J. Grodzinsky
Source:: Biomaterials 2014 v.35 no.1 pp. 538-549
ISSN:: 0142-9612
Subject:: avidin; binding sites; cartilage; collagen; drug carriers; drugs; electrostatic interactions; explants; extracellular matrix; hydrodynamics; models; osteoarthritis; proteoglycans; solutes
Abstract:: ... Local drug delivery into cartilage remains a challenge due to its dense extracellular matrix of negatively charged proteoglycans enmeshed within a collagen fibril network. The high negative fixed charge density of cartilage offers the unique opportunity to utilize electrostatic interactions to augment transport, binding and retention of drug carriers. With the goal of developing particle-based dru ...
DOI:: 10.1016/j.biomaterials.2013.09.091
PubMed:: 24120044
PubMed Central:: PMC3863604
: https://doi.org/10.1016/j.biomaterials.2013.09.091

47. Bacterial (intramembrane-sensing) histidine kinases: signal transfer rather than stimulus perception

Author:: Thorsten Mascher
Source:: Trends in microbiology 2014 v.22 no.10 pp. 559-565
ISSN:: 0966-842X
Subject:: bacteria; extracellular matrix; histidine kinase; membrane proteins; quorum sensing; signal transduction
Abstract:: ... Most membrane-anchored histidine kinases (HKs) of bacterial two-component systems (2CSs) contain an extracellular input domain that is thought to be responsible for sensing an environmental cue. By contrast, intramembrane-sensing HKs (IM-HKs) lack a sensory domain and cannot perceive their stimuli directly. Instead, an N-terminal signal transfer region, consisting solely of two transmembrane helic ...
DOI:: 10.1016/j.tim.2014.05.006
: https://doi.org/10.1016/j.tim.2014.05.006

48. Bacterial expression and functional reconstitution of human heparanase

Author:: Sophie Winkler; Daniela Schweiger; Zheng Wei; Erich Rajkovic; Andreas J. Kungl
Source:: Carbohydrate research 2014 v.389 pp. 72-77
ISSN:: 0008-6215
Subject:: Escherichia coli; angiogenesis; circular dichroism spectroscopy; enzyme inhibitors; extracellular matrix; heparan sulfate; humans; metastasis; screening
Abstract:: ... Human heparanase is a heparan sulfate degrading enzyme located in the extracellular matrix playing a decisive role in angiogenesis and tumor metastasis. Translated as a 65kDa inactive prae-form, the protein is processed into an 8kDa and a 50kDa subunit which form a non-covalently associated active heterodimer. We have expressed the two subunits separately in Escherichia coli which yielded active h ...
DOI:: 10.1016/j.carres.2014.01.002
: https://doi.org/10.1016/j.carres.2014.01.002

49. Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Author:: Tsung-Szu Yeh; Yu-Hua Dean Fang; Chia-Hsin Lu; Shao-Chieh Chiu; Chia-Lin Yeh; Tzu-Chen Yen; Yelena Parfyonova; Yu-Chen Hu
Source:: Biomaterials 2014 v.35 no.1 pp. 174-184
ISSN:: 0142-9612
Subject:: Baculoviridae; blood vessels; cell viability; extracellular matrix; fibrosis; genetic engineering; hypoxia; infarction; models; myocardial infarction; myocardium; physical properties; rabbits; sowing; stem cells; vascular endothelial growth factors
Abstract:: ... Cell sheet technology has been widely employed for the treatment of myocardial infarction (MI), but cell sheet fabrication generally requires the use of thermo-responsive dishes. Here we developed a method for the preparation of adipose-derived stem cell (ASC) sheet that obviated the need of thermo-responsive dishes. This method only required the seeding of rabbit ASC onto 6-well plates at an appr ...
DOI:: 10.1016/j.biomaterials.2013.09.080
: https://doi.org/10.1016/j.biomaterials.2013.09.080

50. Berberine attenuates high glucose-induced proliferation and extracellular matrix accumulation in mesangial cells: Involvement of suppression of cell cycle progression and NF-κB/AP-1 pathways

Author:: Tian Lan; Teng Wu; Cheng Chen; Xiaolan Chen; Jie Hao; Junying Huang; Lijing Wang; Heqing Huang
Source:: Molecular and Cellular Endocrinology 2014 v.384 pp. 109-116
ISSN:: 0303-7207
Subject:: DNA; berberine; cell cycle; cell proliferation; cyclin-dependent kinase; diabetes; extracellular matrix; fibronectins; gel electrophoresis; glucose; hypertrophy; luciferase; phosphorylation; transcription factor NF-kappa B; transforming growth factor beta 1
Abstract:: ... Berberine has been shown to have renoprotective effects on diabetes through attenuating TGF-β1 and fibronectin (FN) expression. However, how berberine regulates TGF-β1 and FN is not fully clear. Here we investigated whether berberine inhibited TGF-β1 and FN expression in high glucose-cultured mesangial cells. Berberine significantly inhibited mesangial cell proliferation and hypertrophy by increas ...
DOI:: 10.1016/j.mce.2014.01.022
: https://doi.org/10.1016/j.mce.2014.01.022

51. Bienzymatically crosslinked gelatin/hyaluronic acid interpenetrating network hydrogels: preparation and characterization

Author:: Zhiping Fan; Yemin Zhang; Shuo Fang; Chen Xu; Xinsong Li
Source:: RSC advances 2014 v.5 no.3 pp. 1929-1936
ISSN:: 2046-2069
Subject:: biocompatibility; biocompatible materials; biological assessment; biopolymers; cell adhesion; compression strength; crosslinking; cytotoxicity; extracellular matrix; gelatin; gelation; hyaluronic acid; hydrogels; peroxidase; protein-glutamine gamma-glutamyltransferase; rheometers; strength (mechanics); tissue engineering; tyramine; water content
Abstract:: ... Hydrogels that mimic the extracellular matrix in both composition and crosslinking chemistry have great potential in the development of tissue engineering scaffolds. Natural polymer hydrogels crosslinked by enzymes have excellent biocompatibility due to their natural origin and the mild reaction conditions, but usually suffer from a lack of mechanical strength. In order to improve the mechanical p ...
DOI:: 10.1039/c4ra12446d
: https://doi.org/10.1039/c4ra12446d

52. Bioactivated protein-based porous microcarriers for tissue engineering applications

Author:: Baiwen Luo; Qiu Li Loh; Marcus Thien Chong Wong; Nguan Soon Tan; Cleo Choong
Source:: Journal of materials chemistry B 2014 v.2 no.44 pp. 7795-7803
ISSN:: 2050-7518
Subject:: alginates; bioactive properties; birds; cell growth; coatings; egg albumen; extracellular matrix; humans; microstructure; ovalbumin; strength (mechanics); surface area; surgery; tissue engineering
Abstract:: ... Microcarriers are commonly used in tissue engineering applications as they provide a large surface area for cell attachment. However, limited research has been done on ovalbumin (OVA), which is a relatively cheap protein found in avian egg white. Hence, in our current study OVA is fabricated into porous microcarriers and the effect of different OVA to alginate ratios on the properties of OVA micro ...
DOI:: 10.1039/c4tb00846d
: https://doi.org/10.1039/c4tb00846d

53. Biofilm dispersion and quorum sensing

Author:: Cristina Solano; Maite Echeverz; Iñigo Lasa
Source:: Current opinion in microbiology 2014 v.18 pp. 96-104
ISSN:: 1369-5274
Subject:: biofilm; cell communication; extracellular matrix; gene expression; group behavior; lifestyle; pathogens; population density; quorum sensing
Abstract:: ... Biofilm development and quorum sensing (QS) are closely interconnected processes. Biofilm formation is a cooperative group behaviour that involves bacterial populations living embedded in a self-produced extracellular matrix. QS is a cell–cell communication mechanism that synchronizes gene expression in response to population cell density. Intuitively, it would appear that QS might coordinate the ...
DOI:: 10.1016/j.mib.2014.02.008
: https://doi.org/10.1016/j.mib.2014.02.008

54. Biofilm-forming ability and resistance to industrial disinfectants of Staphylococcus aureus isolated from fishery products

Author:: Daniel Vázquez-Sánchez; Marta López Cabo; Paula Saá Ibusquiza; Juan José Rodríguez-Herrera
Source:: Food control 2014 v.39 pp. 8-16
ISSN:: 0956-7135
Subject:: Staphylococcus aureus; adhesion; antibacterial properties; antibiotic resistance; benzalkonium chloride; biocides; biofilm; disinfectants; environmental factors; extracellular matrix; fish products; food industry; food processing; gentian violet; minimum inhibitory concentration; peracetic acid; plankton; sodium hypochlorite; staining; stainless steel; temperature
Abstract:: ... Adhesion and biofilm-forming ability of twenty six S. aureus strains previously isolated from fishery products on stainless steel was assessed. All strains reached counts higher than 104CFU/cm2 after 5h at 25°C. Most strains also showed a biofilm-forming ability higher than S. aureus ATCC 6538 – reference strain in bactericidal standard tests – by crystal violet staining. In addition, it seems tha ...
DOI:: 10.1016/j.foodcont.2013.09.029
: https://doi.org/10.1016/j.foodcont.2013.09.029

55. Biofunctionalization of gelatin microcarrier with oxidized hyaluronic acid for corneal keratocyte cultivation

Author:: Jui-Yang Lai
Source:: Colloids and Surfaces B: Biointerfaces 2014 v.122 pp. 277-286
ISSN:: 0927-7765
Subject:: Western blotting; aldehydes; biopolymers; collagen; colloids; cornea; extracellular matrix; gelatin; genes; hyaluronic acid; models; necrosis; nuclear magnetic resonance spectroscopy; phenotype; polystyrenes; rabbits; reverse transcriptase polymerase chain reaction; scanning electron microscopy; sodium; staining; tissue culture
Abstract:: ... Development of microcarriers as alternative architecture models to traditional three-dimensional porous scaffolds can potentially eliminate the concern of extensive geographic necrosis during keratocyte cultivation. For the first time, this work was performed to evaluate the in vitro expansion of rabbit corneal keratocytes (RCKs) on the gelatin microcarrier (GMC) modified with hyaluronic acid (HA) ...
DOI:: 10.1016/j.colsurfb.2014.07.009
: https://doi.org/10.1016/j.colsurfb.2014.07.009

56. Biological materials and molecular biomimetics – filling up the empty soft materials space for tissue engineering applications

Author:: Ali Miserez; James C. Weaver; Ovijit Chaudhuri
Source:: Journal of materials chemistry B 2014 v.3 no.1 pp. 13-24
ISSN:: 2050-7518
Subject:: biocompatible materials; biomimetics; biopolymers; elastin; extensibility; extracellular matrix; models; silk; tissue engineering
Abstract:: ... An extensive range of biomaterials, frequently derived from extracellular matrix (ECM) proteins or other natural biopolymers, have been developed for biomedical applications. Their mechanical response, a key requirement for regenerative medicine, is often stiff but exhibits limited extensibility (e.g. silk), or inversely, is compliant with higher strain to failure (e.g. elastin). While synthetic b ...
DOI:: 10.1039/c4tb01267d
: https://doi.org/10.1039/c4tb01267d

57. Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells

Author:: Christine-Maria Horejs; Andrea Serio; Alan Purvis; Adam J. Gormley; Sergio Bertazzo; Anna Poliniewicz; Alex J. Wang; Peter DiMaggio; Erhard Hohenester; Molly M. Stevens
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.16 pp. 5908-5913
ISSN:: 0027-8424
Subject:: basement membrane; cadherins; cell differentiation; embryonic stem cells; extracellular matrix; gelatinase A; humans; laminin; mice
Abstract:: ... The dynamic interplay between the extracellular matrix and embryonic stem cells (ESCs) constitutes one of the key steps in understanding stem cell differentiation in vitro. Here we report a biologically-active laminin-111 fragment generated by matrix metalloproteinase 2 (MMP2) processing, which is highly up-regulated during differentiation. We show that the β1-chain–derived fragment interacts via ...
DOI:: 10.1073/pnas.1403139111
PubMed:: 24706882
PubMed Central:: PMC4000797
: https://doi.org/10.1073/pnas.1403139111

58. Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

Author:: Lena A. Lautscham; Corey Y. Lin; Vera Auernheimer; Christoph A. Naumann; Wolfgang H. Goldmann; Ben Fabry
Source:: Biomaterials 2014 v.35 pp. 3198-3207
ISSN:: 0142-9612
Subject:: cell adhesion; extracellular matrix; fibroblasts; glass; ligands; lipid bilayers; mechanical properties; polyacrylamide; viscoelasticity
Abstract:: ... Cell behavior such as cell adhesion, spreading, and contraction critically depends on the elastic properties of the extracellular matrix. It is not known, however, how cells respond to viscoelastic or plastic material properties that more closely resemble the mechanical environment cells encounter in the body. In this report, we employ viscoelastic and plastic biomembrane-mimicking cell substrates ...
DOI:: 10.1016/j.biomaterials.2013.12.091
PubMed:: 24439398
PubMed Central:: PMC4026006
: https://doi.org/10.1016/j.biomaterials.2013.12.091

59. Biomimetic ECM coatings for controlled release of rhBMP-2: construction and biological evaluation

Author:: Ying Huang; Qiaojie Luo; Guangyu Zha; Jianxiang Zhang; Xiaohui Li; Shifang Zhao; Xiaodong Li
Source:: Biomaterials science 2014 v.2 no.7 pp. 980-989
ISSN:: 2047-4849
Subject:: adhesion; biological assessment; biomimetics; coatings; collagen; crosslinking; disulfide bonds; disulfides; electrolytes; extracellular matrix; gels; humans; hyaluronic acid; in vivo studies; medical equipment; peptides; therapeutics; tissue repair; torque
Abstract:: ... As we all know biochemical surface modification is promising for implantable biomedical device applications due to its ability to directly provide therapeutic molecular cues for tissue repair. However, presenting multiple molecular cues on implant surfaces in the proper way is challenging. In this study, a multi-component polyelectrolyte multilayer (PEM) coating composed of collagen type I, RGD pe ...
DOI:: 10.1039/c3bm60254k
: https://doi.org/10.1039/c3bm60254k

60. Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals

Author:: Chin Fhong Soon; Wan Ibtisam Wan Omar; Rebecca F. Berends; Nafarizal Nayan; Hatijah Basri; Kian Sek Tee; Mansour Youseffi; Nick Blagden; Morgan Clive Thomas Denyer
Source:: Micron 2014 v.56 pp. 73-79
ISSN:: 0968-4328
Subject:: Fourier transform infrared spectroscopy; atomic force microscopy; cell adhesion; cell culture; cell membranes; cholesterol; collagen; culture media; cultured cells; extracellular matrix; fibronectins; glass; humans; image analysis; integrins; keratinocytes; laminin; liquid crystals; physicochemical properties; surface roughness
Abstract:: ... This study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocyto ...
DOI:: 10.1016/j.micron.2013.10.011
: https://doi.org/10.1016/j.micron.2013.10.011

61. Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic reticulum stress-mediated apoptosis

Author:: Yongjun Zhu; Ruoting Men; Maoyao Wen; Xiaolin Hu; Xiaojing Liu; Li Yang
Source:: Life sciences 2014 v.94 no.1 pp. 37-44
ISSN:: 0024-3205
Subject:: Western blotting; apoptosis; calnexin; caspase-12; cell viability; dose response; endoplasmic reticulum; extracellular matrix; liver cirrhosis; messenger RNA; reverse transcriptase polymerase chain reaction; staining; transmission electron microscopy
Abstract:: ... Proliferation is a ‘multiplier’ for extracellular matrix production and contraction of activated hepatic stellate cells (HSC) in fibrotic liver. Transient receptor potential melastatin-like 7 channels (TRPM7) are implicated in the survival and proliferation of several kinds of cells. This study was aimed to investigate the effect of TRPM7 blocker 2-APB on survival and proliferation of HSC and the ...
DOI:: 10.1016/j.lfs.2013.10.030
: https://doi.org/10.1016/j.lfs.2013.10.030

62. Blood cells transcriptomics as source of potential biomarkers of articular health improvement: effects of oral intake of a rooster combs extract rich in hyaluronic acid

Author:: Juana Sánchez; M. Luisa Bonet; Jaap Keijer; Evert M. van Schothorst; Ingrid Mölller; Carles Chetrit; Daniel Martinez-Puig; Andreu Palou
Source:: Genes & nutrition 2014 v.9 no.5 pp. 417
ISSN:: 1555-8932
Subject:: biomarkers; blood cells; extracellular matrix; gene expression; gene expression regulation; genes; heparan sulfate; humans; hyaluronic acid; metabolism; microarray technology; novel foods; pain; quality of life; roosters; transcriptomics; yogurt; Europe
Abstract:: ... The aim of the study was to explore peripheral blood gene expression as a source of biomarkers of joint health improvement related to glycosaminoglycan (GAG) intake in humans. Healthy individuals with joint discomfort were enrolled in a randomized, double-blind, placebo-controlled intervention study in humans. Subjects ate control yoghurt or yoghurt supplemented with a recently authorized novel fo ...
DOI:: 10.1007/s12263-014-0417-3
PubMed:: 25024048
PubMed Central:: PMC4172652
: https://doi.org/10.1007/s12263-014-0417-3

63. Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Author:: Wei Lu; Kun Ji; Jennifer Kirkham; Yu Yan; Aldo R. Boccaccini; Margaret Kellett; Yan Jin; Xuebin B. Yang
Source:: Cell and tissue research 2014 v.356 no.1 pp. 97-107
ISSN:: 0302-766X
Subject:: alkaline phosphatase; bioactive properties; bone formation; extracellular matrix; foams; humans; immunohistochemistry; mice; patients; polymers; scanning electron microscopy; stem cells; tissue engineering
Abstract:: ... Translational research in bone tissue engineering is essential for “bench to bedside” patient benefit. However, the ideal combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study is to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 wt% and 40 wt% of Bioglass particles wit ...
DOI:: 10.1007/s00441-013-1770-z
PubMed:: 24408074
: https://doi.org/10.1007/s00441-013-1770-z

64. Bone tissue remodeling and development: Focus on matrix metalloproteinase functions

Author:: Katiucia Batista Silva Paiva; José Mauro Granjeiro
Source:: Archives of biochemistry and biophysics 2014 v.561 pp. 74-87
ISSN:: 0003-9861
Subject:: adult stem cells; angiogenesis; animal models; bioactive compounds; bioengineering; biomechanics; bone formation; bone resorption; bones; cartilage; collagenase; extracellular matrix; homeostasis; knockout mutants; neural crest; osteoblasts; osteoclasts; skeletal development; viability
Abstract:: ... Bone-forming cells originate from distinct embryological layers, mesoderm (axial and appendicular bones) and ectoderm (precursor of neural crest cells, which mainly form facial bones). These cells will develop bones by two principal mechanisms: intramembranous and endochondral ossification. In both cases, condensation of multipotent mesenchymal cells occurs, at the site of the future bone, which d ...
DOI:: 10.1016/j.abb.2014.07.034
: https://doi.org/10.1016/j.abb.2014.07.034

65. Bone-Like Mineral Nucleating Peptide Nanofibers Induce Differentiation of Human Mesenchymal Stem Cells into Mature Osteoblasts

Author:: Ceylan Hakan; Kocabey Samet; Unal Gulsuner Hilal; Balcik Ozlem S.; Guler Mustafa O.; Tekinay Ayse B.
Source:: Biomacromolecules 2014 v.15 no.7 pp. 2407-2418
ISSN:: 1526-4602
Subject:: adhesion; bone formation; collagen; extracellular matrix; humans; long term effects; mineralization; mussels; nanofibers; osteoblasts; prostheses; stem cells
Abstract:: ... A bone implant should integrate to the tissue through a bone-like mineralized interface, which requires increased osteoblast activity at the implant–tissue boundary. Modification of the implant surface with synthetic bioinstructive cues facilitates on-site differentiation of progenitor stem cells to functional mature osteoblasts and results in subsequent mineralization. Inspired by the bioactive d ...
DOI:: 10.1021/bm500248r
: https://doi.org/10.1021/bm500248r

66. Bortezomib prevents the expression of MMP-13 and the degradation of collagen type 2 in human chondrocytes

Author:: Weihua Hu; Weikai Zhang; Feng Li; Fengjing Guo; Anmin Chen
Source:: Biochemical and biophysical research communications 2014 v.452 pp. 526-530
ISSN:: 0006-291X
Subject:: cartilage; chondrocytes; collagen; denaturation; extracellular matrix; humans; interferon regulatory factor-1; matrix metalloproteinase 13; myeloma; neoplasm cells; osteoarthritis; patients; proteasome endopeptidase complex; tumor necrosis factor-alpha
Abstract:: ... The structural backbone of extracellular matrix in cartilage is the collagen fibril, which is mainly composed of type II collagen. A measurable increase in type II collagen denaturation and degradation has been found in early Osteoarthritis (OA). Pro-inflammatory cytokine such as TNF-α produced in OA cartilage induced the expression of matrix metalloproteinase-13 (MMP-13), which targets and degrad ...
DOI:: 10.1016/j.bbrc.2014.08.102
: https://doi.org/10.1016/j.bbrc.2014.08.102

67. Breakthroughs spotlighting roles for extracellular nucleotides and apyrases in stress responses and growth and development

Author:: Greg B. Clark; Reginald O. Morgan; Maria-Pilar Fernandez; Mari L. Salmi; Stanley J. Roux
Source:: Plant science 2014 v.225 pp. 107-116
ISSN:: 0168-9452
Subject:: Arabidopsis; abiotic stress; animals; apyrase; auxins; calcium; extracellular matrix; gravitropism; growth retardation; nitric oxide; nucleosides; nucleotides; phylogeny; plant growth; plasma membrane; post-translational modification; secretory granules; stomata; stomatal movement; stress response
Abstract:: ... Animal and plant cells release nucleotides into their extracellular matrix when touched, wounded, and when their plasma membranes are stretched during delivery of secretory vesicles and growth. These released nucleotides then function as signaling agents that induce rapid increases in the concentration of cytosolic calcium, nitric oxide and superoxide. These, in turn, are transduced into downstrea ...
DOI:: 10.1016/j.plantsci.2014.06.002
: https://doi.org/10.1016/j.plantsci.2014.06.002

68. C-20

Author:: Kelvin GM Brockbank
Source:: Cryobiology 2014 v.69 no.3 pp. 507
ISSN:: 0011-2240
Subject:: blood vessels; cartilage; cell viability; cooling; cryopreservation; cryoprotectants; dimethyl sulfoxide; extracellular matrix; humans; ice; immunogenicity; propylene glycol; rabbits; swine; tissue engineering; vitrification; washing
Abstract:: ... Application of the original tissue vitrification method developed by Fahy and used by my research group for rabbit blood vessels and articular cartilage has evolved over time. The formulation was 2.21M propylene glycol, 3.10M formamide and 3.10M dimethylsulfoxide in EuroCollins solution and it was employed with multiple addition/removal steps to minimize osmotic damage to the cells. Ice-free cryop ...
DOI:: 10.1016/j.cryobiol.2014.09.307
: https://doi.org/10.1016/j.cryobiol.2014.09.307

69. C-terminal propeptide is required for fibrillin-1 secretion and blocks premature assembly through linkage to domains cbEGF41-43

Author:: Sacha A. Jensen; Georgia Aspinall; Penny A. Handford
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.28 pp. 10155-10160
ISSN:: 0027-8424
Subject:: Animalia; biomechanics; cytokines; extracellular matrix; fibroblasts; growth factors; protein engineering; secretion
Abstract:: ... Fibrillin microfibrils are 10–12 nm diameter, extracellular matrix assemblies that provide dynamic tissues of metazoan species with many of their biomechanical properties as well as sequestering growth factors and cytokines. Assembly of fibrillin monomers into microfibrils is thought to occur at the cell surface, with initial steps including proprotein processing, multimerization driven by the C t ...
DOI:: 10.1073/pnas.1401697111
PubMed:: 24982166
PubMed Central:: PMC4104899
: https://doi.org/10.1073/pnas.1401697111

70. CD44 in canine leukemia: Analysis of mRNA and protein expression in peripheral blood

Author:: M.E. Gelain; V. Martini; M. Giantin; A. Aricò; A. Poggi; L. Aresu; F. Riondato; M. Dacasto; S. Comazzi
Source:: Veterinary immunology and immunopathology 2014 v.159 no.1-2 pp. 91-96
ISSN:: 0165-2427
Subject:: blood; dogs; exons; extracellular matrix; flow cytometry; fluorescence; gene expression; humans; hyaluronic acid; leukemia; messenger RNA; metastasis; phenotype; prognosis; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:: ... Hyaluronan receptor CD44 mediates interaction between cells and extracellular matrix. The expression of standard form and its variants is dysregulated in human leukemias and is associated with metastasis and prognosis. The aim of this work is the evaluation of CD44 mRNA and protein expression in canine leukemia. Peripheral blood from 20 acute leukemias (AL) (10 acute lymphoblastic, 6 acute myeloid ...
DOI:: 10.1016/j.vetimm.2014.02.008
: https://doi.org/10.1016/j.vetimm.2014.02.008

71. Calorie restriction mimicking effects of roflumilast prevents diabetic nephropathy

Author:: Kulbhushan Tikoo; Saritha Lodea; Pinakin Arun Karpe; Sandeep Kumar
Source:: Biochemical and biophysical research communications 2014 v.450 pp. 1581-1586
ISSN:: 0006-291X
Subject:: albumins; animal disease models; apoptosis; blood; blood glucose; collagen; creatinine; diabetic nephropathy; extracellular matrix; fibronectins; kidneys; low calorie diet; males; oxidative stress; rats; resveratrol; streptozotocin; urea nitrogen
Abstract:: ... Little is known about role of PDE4 in the development and progression of diabetic nephropathy. Here, we investigated the effect of roflumilast, a selective PDE 4 inhibitor in type 1 diabetic nephropathy. Diabetes was induced in male Sprague–Dawley rats using streptozotocin (55mg/kg). Diabetic rats showed elevated plasma glucose, blood urea nitrogen, creatinine and decrease in plasma albumin confir ...
DOI:: 10.1016/j.bbrc.2014.07.039
: https://doi.org/10.1016/j.bbrc.2014.07.039

72. Cannabinoid inhibits HIV-1 Tat-stimulated adhesion of human monocyte-like cells to extracellular matrix proteins

Author:: Erinn S. Raborn; Melissa Jamerson; Francine Marciano-Cabral; Guy A. Cabral
Source:: Life sciences 2014 v.104 no.1-2 pp. 15-23
ISSN:: 0024-3205
Subject:: CD29 antigen; Human immunodeficiency virus 1; actin; adhesion; blood-brain barrier; cannabinoid receptors; cannabinoids; cell adhesion; extracellular matrix; gels; humans; laminin; monocytes; polymerization; therapeutics
Abstract:: ... The aim of this study was to assess the effect of select cannabinoids on human immunodeficiency virus type 1 (HIV-1) transactivating (Tat) protein-enhanced monocyte-like cell adhesion to proteins of the extracellular matrix (ECM).Collagen IV, laminin, or an ECM gel was used to construct extracellular matrix layers. Human U937 monocyte-like cells were exposed to Tat in the presence of ∆⁹-tetrahydro ...
DOI:: 10.1016/j.lfs.2014.04.008
PubMed:: 24742657
PubMed Central:: PMC4089388
: https://doi.org/10.1016/j.lfs.2014.04.008

73. Carbon Quantum Dot Stabilized Gadolinium Nanoprobe Prepared via a One-Pot Hydrothermal Approach for Magnetic Resonance and Fluorescence Dual-Modality Bioimaging

Author:: Xu Yang; Jia Xiao-Hua; Yin Xue-Bo; He Xi-Wen; Zhang Yu-Kui
Source:: Analytical chemistry 2014 v.86 no.24 pp. 12122-12129
ISSN:: 1520-6882
Subject:: Danio rerio; animal models; biocompatibility; carbon; citrates; extracellular matrix; fluorescence; gadolinium; hot water treatment; hydrophilicity; image analysis; in vivo studies; magnetic resonance imaging; neoplasm cells; neoplasms; quantum dots; tissues; toxicity
Abstract:: ... Magnetic resonance imaging (MRI) is used extensively for clinical diagnoses. It is critical to design and develop highly efficient MR contrast agents with simple preparation procedure, low toxicity, and high biocompatibility. Here, we report a carbon quantum dots (CQDs)-stabilized gadolinium hybrid nanoprobe (Gd–CQDs) prepared via a one-pot hydrothermal treatment of the mixture of citrate acid, et ...
DOI:: 10.1021/ac503002c
: https://doi.org/10.1021/ac503002c

74. Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate

Author:: Marina V. Pryzhkova; Indrat Aria; Qingsu Cheng; Greg M. Harris; Xingjie Zan; Morteza Gharib; Ehsan Jabbarzadeh
Source:: Biomaterials 2014 v.35 no.19 pp. 5098-5109
ISSN:: 0142-9612
Subject:: carbon nanotubes; extracellular matrix; humans; plastics; reverse transcriptase polymerase chain reaction; stem cells; surface roughness; tissue culture; tissue engineering; vapors
Abstract:: ... We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our r ...
DOI:: 10.1016/j.biomaterials.2014.03.011
PubMed:: 24690530
PubMed Central:: PMC4943838
: https://doi.org/10.1016/j.biomaterials.2014.03.011

75. Carbon nanotubes leading the way forward in new generation 3D tissue engineering

Author:: Erin Leigh Hopley; Shima Salmasi; Deepak M. Kalaskar; Alexander M. Seifalian
Source:: Biotechnology Advances 2014 v.32 pp. 1000-1014
ISSN:: 0734-9750
Subject:: angiogenesis; biocompatibility; biosensors; blood; carbon nanotubes; cell division; drugs; electrical properties; extracellular matrix; gene expression; humans; medicine; neurodevelopment; polymers; statistics; tensile strength; thrombosis; tissue engineering
Abstract:: ... Statistics from the NHS Blood and Transplant Annual Review show that total organ transplants have increased to 4213 in 2012, while the number of people waiting to receive an organ rose to 7613 that same year. Human donors as the origin of transplanted organs no longer meet the ever-increasing demand, and so interest has shifted to synthetic organ genesis as a form of supply. This focus has given r ...
DOI:: 10.1016/j.biotechadv.2014.05.003
: https://doi.org/10.1016/j.biotechadv.2014.05.003

76. Cardiac outflow tract development relies on the complex function of Sox4 and Sox11 in multiple cell types

Author:: Mandy H. Paul; Richard P. Harvey; Michael Wegner; Elisabeth Sock
Source:: Cellular and molecular life sciences 2014 v.71 no.15 pp. 2931-2945
ISSN:: 1420-682X
Subject:: mechanism of action; neural crest; cell adhesion; transcription factors; humans; congenital abnormalities; cytoskeleton; mice; heart; extracellular matrix
Abstract:: ... Congenital heart defects represent the most common human birth defects and are often life-threatening. Frequently, they are caused by abnormalities of the outflow tract whose formation results from coordinated development of cells from mesodermal and neural crest origin and depends on the activity of many different transcription factors. However, place, time, and mode of action have only been anal ...
DOI:: 10.1007/s00018-013-1523-x
: https://doi.org/10.1007/s00018-013-1523-x

77. Cardiac tissue engineering: renewing the arsenal for the battle against heart disease

Author:: Vassilis Georgiadis; Richard A. Knight; Suwan N. Jayasinghe; Anastasis Stephanou
Source:: Integrative biology 2014 v.6 no.2 pp. 111-126
ISSN:: 1757-9708
Subject:: bioreactors; extracellular matrix; heart; heart diseases; tissue engineering
Abstract:: ... The development of therapies that lead to the regeneration or functional repair of compromised cardiac tissue is the most important challenge facing translational cardiovascular research today. During the last 25 years huge efforts have been made towards restoring the physiologic functions of the heart by means of delivering cell implants into the insulted heart, initially through ‘naked cell’ inj ...
DOI:: 10.1039/c3ib40097b
: https://doi.org/10.1039/c3ib40097b

78. Cardiomyocyte intracellular cholesteryl ester accumulation promotes tropoelastin physical alteration and degradation

Author:: Valerie Samouillan; Elena Revuelta-López; Jany Dandurand; Laura Nasarre; Lina Badimon; Colette Lacabanne; Vicenta Llorente-Cortés
Source:: international journal of biochemistry & cell biology 2014 v.55 pp. 209-219
ISSN:: 1357-2725
Subject:: cardiomyocytes; cathepsin S; elastin; extracellular matrix; glass transition temperature; hyperlipoproteinemia; insulin resistance; low density lipoprotein; protein content; triacylglycerols; very low density lipoprotein
Abstract:: ... Dyslipemia has a direct impact on cardiac remodeling by altering extracellular matrix (ECM) components. One of the main ECM components is elastin, a proteic three-dimensional network that can be efficiently degraded by cysteine proteases or cathepsins. Dyslipemic status in insulin resistance and combined hyperlipoproteinemia diseases include raised levels of very low density lipoproteins (VLDL), t ...
DOI:: 10.1016/j.biocel.2014.09.005
: https://doi.org/10.1016/j.biocel.2014.09.005

79. Cartilage derived morphogenetic protein 2 – A potential therapy for intervertebral disc regeneration?

Author:: Lisa A. Williams; Aiqun Wei; Divya Bhargav; Ashish D. Diwan
Source:: Biologicals 2014 v.42 pp. 65-73
ISSN:: 1045-1056
Subject:: Danio rerio; Xenopus; complications (disease); diabetes; disability-adjusted life year; extracellular matrix; intervertebral disks; mental health; mutation; pain; risk factors; therapeutics; tissue repair
Abstract:: ... Low back pain is amongst the top ten risk factors that contribute to disability, ranking higher than diabetes and mental health disease globally as a contributor to years lost to disability (YLD), and escalating as Western societies age. Abundant evidence suggests that intervertebral disc (IVD) damage is central to the origin of pain in the spine. IVD degeneration involves the progressive deterior ...
DOI:: 10.1016/j.biologicals.2013.12.003
: https://doi.org/10.1016/j.biologicals.2013.12.003

80. Cartilaginous Extracellular Matrix-Modified Chitosan Hydrogels for Cartilage Tissue Engineering

Author:: Choi Bogyu; Kim Soyon; Lin Brian; Wu Benjamin M.; Lee Min
Source:: ACS Applied Materials & Interfaces 2014 v.6 no.22 pp. 20110-20121
ISSN:: 1944-8252
Subject:: biocompatible materials; blue light; cartilage; chitosan; chondrocytes; chondrogenesis; chondroitin sulfate; collagen; encapsulation; extracellular matrix; hydrogels; integrins; riboflavin; stem cells; tissue engineering
Abstract:: ... Cartilaginous extracellular matrix (ECM) components such as type-II collagen (Col II) and chondroitin sulfate (CS) play a crucial role in chondrogenesis. However, direct clinical use of natural Col II or CS as scaffolds for cartilage tissue engineering is limited by their instability and rapid enzymatic degradation. Here, we investigate the incorporation of Col II and CS into injectable chitosan h ...
DOI:: 10.1021/am505723k
: https://doi.org/10.1021/am505723k

81. Celiac disease patient IgA antibodies induce endothelial adhesion and cell polarization defects via extracellular transglutaminase 2

Author:: Cristina Antonella Nadalutti; Ilma Rita Korponay-Szabo; Katri Kaukinen; Martin Griffin; Markku Mäki; Katri Lindfors
Source:: Cellular and molecular life sciences 2014 v.71 no.7 pp. 1315-1326
ISSN:: 1420-682X
Subject:: CD29 antigen; adhesion; angiogenesis; apoptosis; autoantibodies; celiac disease; cell adhesion; cell movement; crosslinking; endothelial cells; enzyme activity; extracellular matrix; fibronectins; immunoglobulin A; patients; phenotype; protein-glutamine gamma-glutamyltransferase; secretion; signal transduction
Abstract:: ... We have recently found that celiac disease patient serum-derived autoantibodies targeted against transglutaminase 2 interfere with several steps of angiogenesis, including endothelial sprouting and migration, though the mechanism involved remained to be fully characterized. This study now investigated the processes underlying the antiangiogenic effects exerted by celiac disease patient antibodies ...
DOI:: 10.1007/s00018-013-1455-5
: https://doi.org/10.1007/s00018-013-1455-5

82. Cell Division Orientation on Biospecific Peptide Gradients

Author:: Lamb Brian M.; Luo Wei; Nagdas Sarbajeet; Yousaf Muhammad N.
Source:: ACS Applied Materials & Interfaces 2014 v.6 no.14 pp. 11523-11528
ISSN:: 1944-8252
Subject:: cell division; extracellular matrix; ligands
Abstract:: ... An assay was developed for determining cell division orientation on gradients. The methodology is based on permeating microfluidic devices with alkanethiols and subsequent printing of cell adhesive peptide gradient self-assembled monolayers (SAMs) for examining oriented cell divisions. To our knowledge, there has been no study examining the correlation between cell division orientations based on a ...
DOI:: 10.1021/am502209k
: https://doi.org/10.1021/am502209k

83. Cell adhesion and invasion inhibitory effect of an ovarian cancer targeting peptide selected via phage display in vivo

Author:: Ximing Pu; Chuying Ma; Guangfu Yin; Fei You; Yan Wei
Source:: Biochemical and biophysical research communications 2014 v.443 pp. 858-863
ISSN:: 0006-291X
Subject:: animal ovaries; bacteriophages; cell adhesion; extracellular matrix; metastasis; neoplasm cells; ovarian neoplasms
Abstract:: ... Organ-specific metastasis is of great importance since most of the cancer deaths are caused by spread of the primary cancer to distant sites. Therefore, targeted anti-metastases therapies are needed to prevent cancer cells from metastasizing to different organs. The phage clone pc3-1 displaying peptide WSGPGVWGASVK selected by phage display had been identified which have high binding efficiency an ...
DOI:: 10.1016/j.bbrc.2013.12.058
: https://doi.org/10.1016/j.bbrc.2013.12.058

84. Cell adhesion mechanisms on laterally mobile polymer films

Author:: Andreas P. Kourouklis; Ronald V. Lerum; Harry Bermudez
Source:: Biomaterials 2014 v.35 no.17 pp. 4827-4834
ISSN:: 0142-9612
Subject:: adhesion; biomimetics; cell adhesion; composite polymers; extracellular matrix; fibroblasts; films (materials); focal adhesions; hydrophobicity; integrins; ligands; quantitative analysis
Abstract:: ... In contrast with the majority of substrates used to study cell adhesion, the natural extracellular matrix (ECM) is dynamic and remodeled over time. Here we use amphiphilic block copolymers to create self-assembled supported films with tunable lateral mobility. These films are intended to serve as partial mimics of the ECM in order to better understand cell adhesion responses, specifically in the c ...
DOI:: 10.1016/j.biomaterials.2014.02.052
: https://doi.org/10.1016/j.biomaterials.2014.02.052

85. Cell–matrix interactions: focus on proteoglycan–proteinase interplay and pharmacological targeting in cancer

Author:: Achilleas D. Theocharis; Chrisostomi Gialeli; Panagiotis Bouris; Efstathia Giannopoulou; Spyros S. Skandalis; Alexios J. Aletras; Renato V. Iozzo; Nikos K. Karamanos
Source:: FEBS journal 2014 v.281 no.22 pp. 5023-5042
ISSN:: 1742-464X
Subject:: extracellular matrix; inflammation; pathophysiology; phenotype; proteinases; proteoglycans; proteolysis; receptors; secretion
Abstract:: ... Proteoglycans are major constituents of extracellular matrices, as well as cell surfaces and basement membranes. They play key roles in supporting the dynamic extracellular matrix by generating complex structural networks with other macromolecules and by regulating cellular phenotypes and signaling. It is becoming evident, however, that proteolytic enzymes are required partners for matrix remodeli ...
DOI:: 10.1111/febs.12927
PubMed:: 25333340
PubMed Central:: PMC5036392
: https://doi.org/10.1111/febs.12927

86. Cerebral small vessel disease-related protease HtrA1 processes latent TGF-β binding protein 1 and facilitates TGF-β signaling

Author:: Nathalie Beaufort; Eva Scharrer; Elisabeth Kremmer; Vanda Lux; Michael Ehrmann; Robert Huber; Henry Houlden; David Werring; Christof Haffner; Martin Dichgans
Source:: Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.46 pp. 16496-16501
ISSN:: 0027-8424
Subject:: arteries; binding proteins; brain; dementia; fibroblasts; mice; pathogenesis; patients; proteolysis; serine proteinases; stroke; transforming growth factor beta
Abstract:: ... High temperature requirement protein A1 (HtrA1) is a primarily secreted serine protease involved in a variety of cellular processes including transforming growth factor β (TGF-β) signaling. Loss of its activity causes cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL), an inherited form of cerebral small vessel disease leading to early-onset strok ...
DOI:: 10.1073/pnas.1418087111
PubMed:: 25369932
PubMed Central:: PMC4246310
: https://doi.org/10.1073/pnas.1418087111

87. Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages

Author:: Leyla Gasimli; Anne Marie Hickey; Bo Yang; Guoyun Li; Mitche dela Rosa; Alison V. Nairn; Michael J. Kulik; Jonathan S. Dordick; Kelley W. Moremen; Stephen Dalton; Robert J. Linhardt
Source:: BBA - General Subjects 2014 v.1840 pp. 1993-2003
ISSN:: 0304-4165
Subject:: biochemical pathways; cell differentiation; embryonic stem cells; enzymes; extracellular matrix; fluorescent antibody technique; heparan sulfate; humans; immunoblotting; proteoglycans
Abstract:: ... Proteoglycans are found on the cell surface and in the extracellular matrix, and serve as prime sites for interaction with signaling molecules. Proteoglycans help regulate pathways that control stem cell fate, and therefore represent an excellent tool to manipulate these pathways. Despite their importance, there is a dearth of data linking glycosaminoglycan structure within proteoglycans with stem ...
DOI:: 10.1016/j.bbagen.2014.01.007
PubMed:: 24412195
PubMed Central:: PMC4004645
: https://doi.org/10.1016/j.bbagen.2014.01.007

88. Changes in the composition of the extracellular matrix accumulated by mesenchymal stem cells during in vitro expansion

Author:: Ryosuke Nakamura; Fumio Nakamura; Shigeharu Fukunaga
Source:: Animal science journal = 2014 v.85 no.6 pp. 706-713
ISSN:: 1344-3941
Subject:: Western blotting; basement membrane; cell culture; collagen; extracellular matrix; fluorescent antibody technique; stem cells
Abstract:: ... One of the approaches to preserve the properties of mesenchymal stem cells (MSCs) during in vitro expansion is to use cell culture substrates. MSCs are known to generate the extracellular matrix (ECM) proper to preserve their proliferative capacity in vitro, but extensive expansion is considered to deprive MSCs of the capacity to prepare such ECM. In order to examine the features of ECM proper tha ...
DOI:: 10.1111/asj.12187
PubMed:: 24612364
: https://doi.org/10.1111/asj.12187

89. Characterisation and assessment of electrospun Poly/hydroxyapatite nanofibres together with a cell adhesive for bone repair applications

Author:: SY Heo; JW Seol; NS Kim
Source:: Veterinární medicína v.59 no.10 pp. 498-501
ISSN:: 0375-8427
Subject:: adhesion; cell adhesion; extracellular matrix; gelatin; hydroxyapatite; nanofibers
Abstract:: ... In this study, we fabricated Poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres using electrospinning and evaluated their potential use for bone repair applications. Analysis confirmed that the PLGA nanofibres were similar to the natural extracellular matrix and included HAp particles. Further, gelatin augmented the adhesion of electrospun nanofibres in the cell adhesion test. Therefo ...
DOI:: 10.17221/7780-vetmed
: https://doi.org/10.17221/7780-vetmed

90. Characterization of PPMUCL1/2/3, Three Members of a New Oomycete-specific Mucin-like Protein Family Residing in Phytophthora parasitica Biofilm

Author:: Marie Larousse; Benjamin Govetto; Aurélie Séassau; Catherine Etienne; Benoit Industri; Nicolas Theodorakopoulos; Emeline Deleury; Michel Ponchet; Franck Panabières; Eric Galiana
Source:: Protist 2014 v.165 no.3 pp. 275-292
ISSN:: 1434-4610
Subject:: Phytophthora nicotianae var. parasitica; biofilm; extracellular matrix; gene expression; genes; glycosylation; immunohistochemistry; nuclear proteins; plant pathogens; protein secretion; serine; staining; threonine; transcriptome
Abstract:: ... The plant pathogen Phytophthora parasitica forms a biofilm on the host surface. The biofilm transcriptome is characterized by the expression of PPMUCL1/2/3 (PHYTOPHTHORA PARASITICA MUCIN-LIKE) genes, which we report here to be members of a new, large mucin-like gene family restricted to the oomycete lineage. These genes encode secreted proteins organized into two domains. The NH2-terminal domain i ...
DOI:: 10.1016/j.protis.2014.03.003
: https://doi.org/10.1016/j.protis.2014.03.003

91. Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae

Author:: Estelle Deniaud-Bouët; Nelly Kervarec; Gurvan Michel; Thierry Tonon; Bernard Kloareg; Cécile Hervé
Source:: Annals of botany 2014 v.114 no.6 pp. 1203-1216
ISSN:: 0305-7364
Subject:: Fucales; alginates; cell fractionation; cell wall components; cell walls; cellulose; chromatography; crosslinking; evolution; extracellular matrix; fucoidan; osmotic stress; phenols; polymers; proteins
Abstract:: ... Background and Aims Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall. They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates). How these components are organized into a three-dimensional extracellular matrix (ECM) still remains un ...
DOI:: 10.1093/aob/mcu096
PubMed:: 24875633
PubMed Central:: PMC4195554 [Available on 10/01/15]
: https://doi.org/10.1093/aob/mcu096

92. Chitosan/silk fibroin-based, Schwann cell-derived extracellular matrix-modified scaffolds for bridging rat sciatic nerve gaps

Author:: Yun Gu; Jianbin Zhu; Chengbin Xue; Zhenmeiyu Li; Fei Ding; Yumin Yang; Xiaosong Gu
Source:: Biomaterials 2014 v.35 no.7 pp. 2253-2263
ISSN:: 0142-9612
Subject:: biocompatible materials; blood; chitosan; electrophysiology; extracellular matrix; fibroins; histopathology; nerve tissue; rats; silk; tissue engineering; tissue repair; tissue transplantation
Abstract:: ... Extracellular matrix (ECM) plays a prominent role in establishing and maintaining an ideal microenvironment for tissue regeneration, and ECM scaffolds are used as a feasible alternative to cellular and molecular therapy in the fields of tissue engineering. Because of their advantages over tissue-derived ECM scaffolds, cultured cell-derived ECM scaffolds are beginning to attract attention, but they ...
DOI:: 10.1016/j.biomaterials.2013.11.087
: https://doi.org/10.1016/j.biomaterials.2013.11.087

93. Chondroitin sulphate extracted from antler cartilage using high hydrostatic pressure and enzymatic hydrolysis

Author:: Chong-Tai Kim; Naiyana Gujral; Advaita Ganguly; Joo-Won Suh; Hoon H. Sunwoo
Source:: Biotechnology reports 2014 v.4 pp. 14-20
ISSN:: 2215-017X
Subject:: 2,2-diphenyl-1-picrylhydrazyl; Cervus; Western blotting; antioxidant activity; biotechnology; cartilage; chondroitin sulfate; chromatography; enzymatic hydrolysis; extracellular matrix; high pressure treatment; hyaluronic acid; monoclonal antibodies; pH; papain; uronic acids; velvet antlers
Abstract:: ... Chondroitin sulphate (CS), a major glycosaminoglycan, is an essential component of the extracellular matrix in cartilaginous tissues. Wapiti velvet antlers are a rich source of these molecules. The purpose of the present study was to develop an effective isolation procedure of CS from fresh velvet antlers using a combination of high hydrostatic pressure (100MPa) and enzymatic hydrolysis (papain). ...
DOI:: 10.1016/j.btre.2014.07.004
PubMed:: 28626657
PubMed Central:: PMC5466122
: https://doi.org/10.1016/j.btre.2014.07.004

94. Cilostazol prevents the degradation of collagen Type II in human chondrocytes

Author:: Weidong Wang; Weibiao Kang; Qiang Tang; Guanfeng Yao; Yuchun Chen; Bizhen Cheng; Kangmei Kong
Source:: Biochemical and biophysical research communications 2014 v.451 pp. 352-355
ISSN:: 0006-291X
Subject:: Food and Drug Administration; biomechanics; cartilage; chondrocytes; collagen; disease course; drug therapy; extracellular matrix; humans; inflammation; interferon regulatory factor-1; interleukin-1beta; osteoarthritis; phosphorylation; signal transduction; transactivators; tyrosine
Abstract:: ... The alteration of extracellular matrix (ECM) in cartilage during the pathological development of Osteoarthritis (OA) changes the biomechanical environment of chondrocytes, which further drives the progression of the disease in the presence of inflammation. Healthy cartilage matrix mainly contains collagen type II, which is degraded by matrix metalloproteinase13 (MMP13), an important molecules resp ...
DOI:: 10.1016/j.bbrc.2014.07.058
: https://doi.org/10.1016/j.bbrc.2014.07.058

95. Cleavage of the Drosophila screw prodomain is critical for a dynamic BMP morphogen gradient in embryogenesis

Author:: Jaana Künnapuu; Petra M. Tauscher; Nina Tiusanen; Minh Nguyen; Ari Löytynoja; Kavita Arora; Osamu Shimmi
Source:: Developmental biology 2014 v.389 no.2 pp. 149-159
ISSN:: 0012-1606
Subject:: Drosophila; alleles; bone morphogenetic proteins; embryogenesis; extracellular matrix; ligands; mutation; phenotype; secretion
Abstract:: ... Dorsoventral patterning of the Drosophila embryo is regulated by graded distribution of bone morphogenetic proteins (BMPs) composed of two ligands, decapentaplegic (Dpp) a BMP2/4 ortholog and screw (Scw) a BMP5/6/7/8 family member. scwᴱ¹ encodes an unusual allele that was isolated as a dominant enhancer of partial loss-of-function mutations in dpp. However, the molecular mechanisms that underlie t ...
DOI:: 10.1016/j.ydbio.2014.02.007
: https://doi.org/10.1016/j.ydbio.2014.02.007

96. Co-delivery of Cbfa-1-targeting siRNA and SOX9 protein using PLGA nanoparticles to induce chondrogenesis of human mesenchymal stem cells

Author:: Su Yeon Jeon; Ji Sun Park; Han Na Yang; Hye Jin Lim; Se Won Yi; Hansoo Park; Keun-Hong Park
Source:: Biomaterials 2014 v.35 no.28 pp. 8236-8248
ISSN:: 0142-9612
Subject:: bioactive properties; bone formation; cell differentiation; chondrocytes; chondrogenesis; circular dichroism spectroscopy; extracellular matrix; humans; injection site; messenger RNA; mice; nanoparticles; protein content; small interfering RNA; staining; stem cells; transcription factors
Abstract:: ... During stem cell differentiation, various cellular responses occur that are mediated by transcription factors and proteins. This study evaluated the abilities of SOX9, a crucial protein during the early stage of chondrogenesis, and siRNA targeting Cbfa-1, a transcription factor that promotes osteogenesis, to stimulate chondrogenesis. Non-toxic poly-(d,l-lactide-co-glycolide) (PLGA) nanoparticles ( ...
DOI:: 10.1016/j.biomaterials.2014.05.092
: https://doi.org/10.1016/j.biomaterials.2014.05.092

97. Collagen-functionalised titanium surfaces for biological sealing of dental implants: Effect of immobilisation process on fibroblasts response

Author:: Nathalia Marín-Pareja; Emiliano Salvagni; Jordi Guillem-Marti; Conrado Aparicio; Maria-Pau Ginebra
Source:: Colloids and Surfaces B: Biointerfaces 2014 v.122 pp. 601-610
ISSN:: 0927-7765
Subject:: X-ray photoelectron spectroscopy; adhesion; biomimetics; chemical bonding; collagen; colloids; dental prosthesis; extracellular matrix; fibroblasts; fluorescent labeling; gene overexpression; genes; humans; oxygen; peptides; titanium; wettability
Abstract:: ... The clinical success of a dental implant requires not only an optimum osseointegration, but also the development of a biological sealing; i.e., a soft tissue seal around the transmucosal part of the implant. A promising approach to improve the biological seal of dental implants is the biomimetic modification of titanium surfaces with proteins or peptides that have specific cell-binding moieties. I ...
DOI:: 10.1016/j.colsurfb.2014.07.038
: https://doi.org/10.1016/j.colsurfb.2014.07.038

98. Colonization of the meat extracellular matrix proteins by O157 and non-O157 enterohemorrhagic Escherichia coli

Author:: Caroline Chagnot; Nelly Caccia; Estelle Loukiadis; Sarah Ganet; Alexandra Durand; Yolande Bertin; Régine Talon; Thierry Astruc; Mickaël Desvaux
Source:: International journal of food microbiology 2014 v.188 pp. 92-98
ISSN:: 0168-1605
Subject:: European Union; Shiga toxin; bacterial adhesion; bacterial colonization; beef; biofilm; centrifugation; enterohemorrhagic Escherichia coli; environmental factors; extracellular matrix; food matrix; food pathogens; food processing; human health; poisoning; proteins; risk; serotypes; skeletal muscle; slaughter
Abstract:: ... Enterohemorrhagic Escherichia coli (EHEC) are anthropozoonotic agents that range third among food-borne pathogens respective to their incidence and dangerousness in the European Union. EHEC are Shiga-toxin producing E. coli (STEC) responsible for foodborne poisoning mainly incriminated to the consumption of contaminated beef meat. Among the hundreds of STEC serotypes identified, EHEC mainly belong ...
DOI:: 10.1016/j.ijfoodmicro.2014.07.016
: https://doi.org/10.1016/j.ijfoodmicro.2014.07.016

99. Comparative Study of Transforming Growth Factor-β Signalling and Regulatory Molecules in Human and Canine Idiopathic Pulmonary Fibrosis

Author:: L. Lilja-Maula; P. Syrjä; H.P. Laurila; E. Sutinen; M. Rönty; K. Koli; M.M. Rajamäki; M. Myllärniemi
Source:: Journal of comparative pathology 2014 v.150 no.4 pp. 399-407
ISSN:: 0021-9975
Subject:: binding proteins; dog diseases; dogs; epithelium; extracellular matrix; fibrosis; histopathology; humans; immunohistochemistry; lungs; pathophysiology; people; pneumonia; regulatory proteins; terriers; transforming growth factor beta
Abstract:: ... Activation of transforming growth factor (TGF)-β is a key event in the progression of fibrosis in human lung tissue. Idiopathic pulmonary fibrosis (IPF) in West Highland white terriers (WHWTs) shares histopathological features of human usual interstitial pneumonia (UIP), the histopathological counterpart of IPF and non-specific interstitial pneumonia (NSIP). The aim of the present immunohistochemi ...
DOI:: 10.1016/j.jcpa.2013.12.001
: https://doi.org/10.1016/j.jcpa.2013.12.001

100. Comparative decellularization and recellularization of normal versus emphysematous human lungs

Author:: Darcy E. Wagner; Nicholas R. Bonenfant; Charles S. Parsons; Dino Sokocevic; Elice M. Brooks; Zachary D. Borg; Melissa J. Lathrop; John D. Wallis; Amanda B. Daly; Ying Wai Lam; Bin Deng; Michael J. DeSarno; Takamaru Ashikaga; Roberto Loi; Daniel J. Weiss
Source:: Biomaterials 2014 v.35 pp. 3281-3297
ISSN:: 0142-9612
Subject:: cell growth; electron microscopy; epithelial cells; extracellular matrix; fibroblasts; humans; immunohistochemistry; lungs; mass spectrometry; patients; respiratory tract diseases; solubilization; staining; stem cells; tissue culture; tissue engineering
Abstract:: ... Acellular whole human lung scaffolds represent a unique opportunity for ex vivo tissue engineering. However, it remains unclear whether lungs from individuals with chronic lung diseases such as chronic obstructive pulmonary disease (COPD) can be appropriately decellularized and recellularized. To assess this, cadaveric human lungs from normal (non-smoking) patients and from patients with COPD (smo ...
DOI:: 10.1016/j.biomaterials.2013.12.103
PubMed:: 24461327
PubMed Central:: PMC4215725
: https://doi.org/10.1016/j.biomaterials.2013.12.103

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1. (±)-Aspongamide A, an N-Acetyldopamine Trimer Isolated from the Insect Aspongopus chinensis, Is an Inhibitor of p-Smad3

2. 1H NMR spectroscopic studies establish that heparanase is a retaining glycosidase

3. 2,5-Dimethoxy 2,5-dihydrofuran crosslinked chitosan fibers enhance bone regeneration in rabbit femur defects

4. 3D Tissue Engineered Supramolecular Hydrogels for Controlled Chondrogenesis of Human Mesenchymal Stem Cells

5. A 3-D in vitro co-culture model of mammary gland involution

6. A 3D matrix platform for the rapid generation of therapeutic anti-human carcinoma monoclonal antibodies

7. A Dictyostelium cellobiohydrolase orthologue that affects developmental timing

8. A Nacre Protein, n16.3, Self-Assembles To Form Protein Oligomers That Dimensionally Limit and Organize Mineral Deposits

9. A Nanostructured Synthetic Collagen Mimic for Hemostasis

10. A Sporulation Factor Is Involved in the Morphological Change of Clostridium perfringens Biofilms in Response to Temperature

11. A Study of Phenylalanine Side-Chain Dynamics in Surface-Adsorbed Peptides Using Solid-State Deuterium NMR and Rotamer Library Statistics

12. A cell-assembled, spatially aligned extracellular matrix to promote directed tissue development

13. A chondromimetic microsphere for in situ spatially controlled chondrogenic differentiation of human mesenchymal stem cells

14. A discoidin domain receptor 1 knock-out mouse as a novel model for osteoarthritis of the temporomandibular joint

15. A genome‐wide association study for canine cryptorchidism in Siberian Huskies

16. A modular approach to easily processable supramolecular bilayered scaffolds with tailorable properties

17. A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

18. A novel motif in the NaTrxh N-terminus promotes its secretion, whereas the C-terminus participates in its interaction with S-RNase in vitro

19. A robust pH-sensitive drug carrier: Aqueous micelles mineralized by calcium phosphate based on chitosan

20. A role for carbohydrate recognition in mammalian sperm-egg binding

21. A shape-controlled tuneable microgel platform to modulate angiogenic paracrine responses in stem cells

22. A spectrophotometer-based diffusivity assay reveals that diffusion hindrance of small molecules in extracellular matrix gels used in 3D cultures is dominated by viscous effects

23. Absence of feedback regulation in the synthesis of COL1A1

24. Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin

25. Age-related disruption of autophagy in dermal fibroblasts modulates extracellular matrix components

26. Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent

27. Aggrecan is required for growth plate cytoarchitecture and differentiation

28. Akt induces down regulation of MUC5AC production in NCI-H292 human airway epithelial cells cultured on extracellular matrix

29. Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gels

30. Aligning cells in arbitrary directions on a membrane sheet using locally formed microwrinkles

31. Alteration of energy metabolism gene expression in cumulus cells affects oocyte maturation via MOS–mitogen-activated protein kinase pathway in dairy cows with an unfavorable “Fertil−” haplotype of one female fertility quantitative trait locus

32. Amelioration of diabetic nephropathy by orange peel extract in rats

33. Amphiphilic beads as depots for sustained drug release integrated into fibrillar scaffolds

34. An RNA interference based study for the role of ALDH1 in keratinocytes: DNA microarray, antibody–chip array and bioinformatics approaches

35. An ectopic approach for engineering a vascularized tracheal substitute

36. Angiogenesis in Bone Regeneration: Tailored Calcium Release in Hybrid Fibrous Scaffolds

37. AngiotensinII induces HuR shuttling by post-transcriptional regulated CyclinD1 in human mesangial cells

38. Anti-fibrotic effect of thymoquinone on hepatic stellate cells

39. Anti-inflammatory and Chondroprotective Activity of (+)-α-Pinene: Structural and Enantiomeric Selectivity

40. Anti-wrinkle effects of fermented and non-fermented Cyclopia intermedia in hairless mice

41. Antibiotic resistance in Pseudomonas aeruginosa biofilms: Towards the development of novel anti-biofilm therapies

42. Association of prohibitin-1 and 2 with oxidative stress in rats with renal interstitial fibrosis

43. Association of the SNP rs2623047 in the HSPG modification enzyme SULF1 with an Australian Caucasian Breast Cancer Cohort

44. Attapulgite-doped electrospun poly(lactic-co-glycolic acid) nanofibers enable enhanced osteogenic differentiation of human mesenchymal stem cells

45. Augmentation of integrin-mediated mechanotransduction by hyaluronic acid

46. Avidin as a model for charge driven transport into cartilage and drug delivery for treating early stage post-traumatic osteoarthritis

47. Bacterial (intramembrane-sensing) histidine kinases: signal transfer rather than stimulus perception

48. Bacterial expression and functional reconstitution of human heparanase

49. Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

50. Berberine attenuates high glucose-induced proliferation and extracellular matrix accumulation in mesangial cells: Involvement of suppression of cell cycle progression and NF-κB/AP-1 pathways

51. Bienzymatically crosslinked gelatin/hyaluronic acid interpenetrating network hydrogels: preparation and characterization

52. Bioactivated protein-based porous microcarriers for tissue engineering applications

53. Biofilm dispersion and quorum sensing

54. Biofilm-forming ability and resistance to industrial disinfectants of Staphylococcus aureus isolated from fishery products

55. Biofunctionalization of gelatin microcarrier with oxidized hyaluronic acid for corneal keratocyte cultivation

56. Biological materials and molecular biomimetics – filling up the empty soft materials space for tissue engineering applications

57. Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells

58. Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate

59. Biomimetic ECM coatings for controlled release of rhBMP-2: construction and biological evaluation

60. Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals

61. Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic reticulum stress-mediated apoptosis

62. Blood cells transcriptomics as source of potential biomarkers of articular health improvement: effects of oral intake of a rooster combs extract rich in hyaluronic acid

63. Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

64. Bone tissue remodeling and development: Focus on matrix metalloproteinase functions

65. Bone-Like Mineral Nucleating Peptide Nanofibers Induce Differentiation of Human Mesenchymal Stem Cells into Mature Osteoblasts

66. Bortezomib prevents the expression of MMP-13 and the degradation of collagen type 2 in human chondrocytes

67. Breakthroughs spotlighting roles for extracellular nucleotides and apyrases in stress responses and growth and development

68. C-20

69. C-terminal propeptide is required for fibrillin-1 secretion and blocks premature assembly through linkage to domains cbEGF41-43

70. CD44 in canine leukemia: Analysis of mRNA and protein expression in peripheral blood