An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... Almond dried-fruit consumption is a characteristic of the Mediterranean diet and the related transformation industry could represent a great economic chance for Sicily, the main producing Italian region. Nevertheless, the Italian almond market is suffering a long crisis caused by the international competition with low-cost producing countries. For this purpose, the Sicilian Region funded a multidi ...
... One new naturally occurring 7-membered 2,5-dioxopiperazine alkaloid named (+)-cyclopenol (1), along with nine known compounds including viridicatol (2), 3-(dimethylaminomethyl)-1-(1,1-dimethyl-2-propenyl)indole (3), anacine (4), aurantiomide C (5), viridicatin (6), 3- O -methylviridicatin (7), verrucosidin (8), ergosterol (9), and ergosterol peroxide (10), was isolated from the EtOAc extract of fu ...
... Penicillium digitatum (Pers.:Fr.) Sacc., the causal agent of citrus green mold, is the main postharvest pathogen of citrus fruit. The control of this pathogen relies on the use of synthetic chemical fungicides. The long-term use of a few authorized fungicides, however, has led to the appearance of resistant strains worldwide. In the last few decades, a great effort has been invested in the search ...
... 1,2-Dihydroxymintlactone (1), a new monoterpene possessing nematicidal activity was isolated as a minor metabolite from the culture filtrate of the Basidiomycete Cheimonophyllum candidissimum , a fungus that previously has yielded nematicidal sesquiterpenes. The structure was determined by spectroscopic methods. ...
... The ¹³C resonance spectra of fungal melanins from Aspergillu sniger, Eurotium echinolatum , and Stachybotrys chartarum are reported. The spectra were taken in 5% W/W solution of the substances in 0.1 ɴ NaOD in D₂O using the Fourier-transform-technique. The spectra and possible as signments of the bands are discussed. The similarities of these spectra to those of soil humic acids are unexpectedly ...
... We investigated the effect of 2,6‐dimethoxy‐1,4‐benzoquinone (DMBQ) on induced resistance to Magnaporthe oryzae in rice. DMBQ concentrations greater than 50 μg/ml inhibited spore germination and appressorium formation in M. oryzae. When rice leaves pretreated with 10 μg/ml DMBQ, which did not show antifungal activity against spore germination and appressorium formation of M. oryzae, were inoculate ...
... Two-year field examinations to see effects of the principle components of a fungus causing “fairy rings” phenomena on lawns, 2-azahypoxanthine (AHX) and imidazole-4-carboxamide (ICA), on wheat were performed. The treatment of AHX or ICA was performed, in the early (seedling treatment, seed soaking treatment) and late (field treatment) developmental stages, and the resulting number of ears and grai ...
carbon; cytotoxicity; fungi; nuclear magnetic resonance spectroscopy; spectral analysis; Cameroon
Abstract:
... Eight new 2-pentenedioic acid derivatives (1–8), and one known congener, 2-decanyl-2-pentenedioic acid (9), were isolated from a soil-derived fungus Gongronella butleri collected in Cameroon. The structures of the new compounds were established by spectroscopic methods including 1D, 2D NMR, and MS. The isolated compounds feature a 2-pentenedioic acid core structure substituted by a 2-alkyl chain t ...
... Root growth promoters, 4-hydroxykigelin (1) and 6-demethylkigelin (2), together with 6- hydroxymellein (3) were isolated from cultures of the fungus Aspergillus terreus and their structures were identified by spectroscopic analysis. The biological activities of the three dihydroisocoumarins, 1, 2, and 3, have been examined using a bioassay method with lettuce seedlings. Furthermore, interactions b ...
... Two silver 5,5-diethylbarbiturate (barb) complexes with 2,2'-bipyridine (bpy) and 3-(2-pyridyl) propanol (pypr), [Ag(barb)(bpy)] (1) and [Ag(barb)(pypr)] (2), have been prepared and characterized by elemental analysis, IR spectroscopy, thermal analysis, and single crystal X-ray diffraction. Both complexes crystallize in the triclinic space group P1 with Z = 2. The barb ligand in 1 is N-coordinated ...
... Fermentation of a mixture of 3,17α,21-triacetoxy-3,5-pregna-diene-20-one and 17α ,21- diacetoxy-4-pregnene-3,20-dione with Flavobacterium dehydrogenans ATCC 13930 led to 17α-acetoxy-21-hydroxy-4-pregnene-3,20-dione as the main product and the novel 17 aacetoxy- 6β,21-dihydroxy-4-pregnene-3,20-dione and 17α-acetoxy- 6α ,21-dihydroxy-4-pregnene- 3,20-dione as the minor ones. 6 -Hydroxylation was not ...
... 7-O-Methyl-luteone was metabolized in a buffer solution by resting cells of Botrytis cinerea which is known as a fungus detoxifying prenylated isoflavones into further oxygenated metabolites, and we could isolate an unstable epoxy-intermediate. The epoxy-intermediate was easily cyclized under acidic conditions to yield the corresponding isoflavone with a hydroxychroman part structure (= dihydropyr ...
Vital Vialas; Zhi Sun; Carla Verónica Loureiro y Penha; Montserrat Carrascal; Joaquín Abián; Lucía Monteoliva; Eric W. Deutsch; Ruedi Aebersold; Robert L. Moritz; Concha Gil
Candida albicans; Internet; apoptosis; data collection; databases; dimorphism; fungi; monitoring; open reading frames; pathogenesis; pathogens; peptides; proteins; proteome; proteomics; virulence
Abstract:
... Candida albicans public proteomic datasets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22,000 distinct peptides at a 0.24% False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained cover ...
... Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) is a recently described inflammatory condition of domestic cats with unknown aetiology. A proportion of cases of FGESF are associated with bacteria, but antibiotic treatment is ineffective. It has been hypothesized that genetically predisposed cats may develop FGESF in response to the introduction of bacteria or other antigens int ...
... Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes. Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular me ...
Alain Roussel; Sawsan Amara; Antti Nyyssölä; Eduardo Mateos-Diaz; Stéphanie Blangy; Hanna Kontkanen; Ann Westerholm-Parvinen; Frédéric Carrière; Christian Cambillau
Trichoderma reesei; active sites; cutin; cutinase; detergents; fungi; galactolipids; long chain triacylglycerols; pH; phospholipids; serine; surfactants
Abstract:
... Cutinases belong to the α/β-hydrolase fold family of enzymes and degrade cutin and various esters, including triglycerides, phospholipids and galactolipids. Cutinases are able to degrade aggregated and soluble substrates because, in contrast with true lipases, they do not have a lid covering their catalytic machinery. We report here the structure of a cutinase from the fungus Trichoderma reesei (T ...
... Helminthosporium solani is a slow-growing fungal pathogen belonging to the family Massarinaceae. It causes blemishes on potato tubers, affecting processing and fresh market trade. Despite its worldwide distribution, little is known about the biology of H. solani. Here we report the generation of a draft genome sequence of H. solani with an estimated genome size of ~35 megabases, for use in phyloge ...
DNA; DNA fragmentation; DNA primers; Puccinia graminis; Puccinia recondita; Puccinia striiformis; Triticum aestivum; amplified fragment length polymorphism; detection limit; disease control; disease outbreaks; fungi; hyphae; leaves; microbial detection; microsatellite repeats; pathogens; stem rust; wheat; China
Abstract:
... Stem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), has historically caused severe losses to wheat (Triticum aestivum L.) production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wheat stem rust have occurred, which caused destructive losses of wheat between 1949 and 1966, although these ...
... An elicitor of the lignification response in wheat leaves was isolated from the germ-tube walls of wheat stem rust. The active compound causes metabolic changes typically correlated with the resistance response, i.e. the formation of lignin or lignin-like polymers in affected epidermal and mesophyll cells and the increased activities of enzymes involved in the phenylpropanoid-pathway. ...
... Phylloplanins are defensive glycoproteins secreted onto leaf surfaces by trichome-bearing plants such as tobacco (Nicotiana tabacum). They are of interest because of their antimicrobial properties, but like other natural product bioactives, the assessment and screening of phylloplanins biological activity is impeded by limited availabilities of active compounds. Here we report an inexpensive micro ...
... Relationships among cavity-nesting birds, trees, and wood decay fungi pose interesting management challenges and research questions in many systems. Ornithologists need to understand the relationships between cavitynesting birds and fungi in order to understand the habitat requirements of these birds. Typically, researchers rely on fruiting body surveys to identify the fungal players in these rela ...
Gram-negative bacteria; antibacterial properties; chemical degradation; fungi; metabolites; seagrasses; spectral analysis; Egypt; Red Sea
Abstract:
... A new 8-hydroxy flavone O-xyloside sulfate, isoscutellarein 7-O-β-xyloside-2′′-O-sulfate, along with four known metabolites, were isolated and identified from seagrass, Thalassia hemprichii, collected from the Egyptian Red sea coast. The structures of the isolated compounds were established by chemical degradation and spectral analysis. The crude extract and the new compound displayed different po ...
... One new anthraquinone derivative, named 8-O-methylnidurufin (1), together with five known analogues (2–6), have been isolated from a gorgonian-derived fungus, Aspergillus sp. The structures were elucidated by combined spectroscopic methods including 1D and 2D NMR spectral data. All isolated metabolites were evaluated for their cytotoxic and antibacterial activities in vitro. Compound 2 showed sign ...
... A new aurone 1 and two known substances, aurantiamide acetate (2) and tiliroside (3), were isolated from the ethanolic extract of Gomphrena agrestis. The structural determination of 1 was based on spectroscopic and spectrometric data. The substance was defined as (E)-3′- O-β-ᴅ-glucopyranosyl-4,5,6,4′-tetrahydroxy-7,2′-dimethoxyaurone. Biological activity of the ethanolic crude extract and isolated ...
Rejane C. de Souza; João B. Fernandes; Paulo C. Vieira; M. Fátima das G. F. da Silva; Marizete F. P. Godoy; Fernando C. Pagnocca; Odair C. Bueno; M. José A. Hebling; José R. Pirani
... The stems of Pilocarpus grandiflorus have afforded the new imidazole alkaloid 4,6-dehydro- 1,2,4,5-tetrahydro-2,5-dioxopilocarpine in addition to the 17 known compounds germanicol, β - amiryn, ocotillone, stigmast-4-en-3-one, 3β -hydroxy-stigmast-5-en-7-one, 6β -hydroxy-stigmast-4- en-3-one, β -sitosterol, scopoletin, 3-(1’,1’-dimethylallyl)-scopoletin, elisin, dictamine, 4-methoxy-2- quinolone, p ...
... From the mycelium of the fungus Aspergillus quadrilineatus, a new pigment 7-methoxyaverufin has been isolated besides mannitol, sterigmatocystin, versicolorin, ergosterol, and averufin. ...
... A hitherto unidentified endophytic strain of the genus Chaetomium, isolated from the medicinal plant Otanthus maritimus, yielded a new tetrahydrofuran derivative, aureonitolic acid (), along with 5 known natural products, 2 - 6. The structure of 1 was determined by extensive spectroscopic analysis and comparison with reported data. Extracts of the fungus, grown either in liquid culture or on solid ...
active ingredients; anti-infective agents; antimicrobial properties; bacteria; chemical defenses; chromatography; ethyl acetate; fungi; horseradish; peroxidase; phenolic compounds; plant pathogens; spectroscopy
Abstract:
... Syringaldehyde readily reacted in the horse-radish peroxidase (HRPOD) system. The ethyl acetate extract of the reaction mixture showed a marked antimicrobial activity against bacteria and fungi. After repeated column chromatography three potential antimicrobial compounds were obtained from the extract. The structural elucidation of active compounds was achieved by a combination of spectroscopic te ...
... Seeds contaminated with pathogens are the primary inoculum for plant diseases in many food crops. Conventional treatments for seedborne diseases use hot water, chlorine or fungicide applications. A novel seed treatment method based on non-thermal plasma generated by an air dielectric barrier discharge (DBD) device was evaluated in this study as an alternative to these conventional treatments. The ...
... Class II fructose 1,6-bisphosphate aldolase (FBA) is an enzyme critical for bacterial, fungal, and protozoan glycolysis/gluconeogenesis. Importantly, humans lack this type of aldolase, having instead a class I FBA that is structurally and mechanistically distinct from class II FBAs. As such, class II FBA is considered a putative pharmacological target for the development of novel antibiotics again ...
... Populus spp. and their hybrids are short-rotation woody crops which supply fiber to a diversity of industries in North America. The potential of hybrid poplar trees has been limited by the fungal pathogen Septoria musiva, the cause of leaf spot and stem canker of Populus spp. An inoculation protocol that does not rely on stem wounding to achieve infection was recently developed to screen poplar cl ...
... Salicorn 46, an endophytic fungus isolated from Salicornia herbacea Torr., was identified as Penicillium citrinum based on its internal transcribed spacer and ribosomal large-subunit DNA sequences using a type I polyketide synthase (PKS I) gene screening approach. A new polyketide, penicitriketo (1), and seven known compounds, including ergone (2), (3β,5α,8α,22E)-5,8-epidioxyergosta-6,9,22-trien-3 ...
... A new polyketide, fusaequisin A (1), was isolated from rice cultures of Fusarium equiseti SF-3-17, an endophytic fungus previously discovered during screening of medicinal plants in Cameroon. The structure of 1 was determined using spectroscopic methods. Fusaequisin A (1) exhibited moderate antimicrobial activity against Staphylococcus aureus NBRC 13276 and Pseudomonas aeruginosa ATCC 15442 at a c ...
... LsGRP1 is a defense-related gene differentially expressed in lily leaves in response to pathogen attack. The difficulty in the expression of LsGRP1 in Escherichia coli suggested the presence of antimicrobial activity in LsGRP1. To evaluate the antimicrobial trait of LsGRP1, three LsGRP1-derived peptides were chemically synthesized; namely LsGRP1ᴺ (N-terminal region without the signal peptide), LsG ...
... The cyclic tetrapeptide 1-alaninechlamydocin was purified from a Great Lakes-derived fungal isolate identified as a Tolypocladium sp. Although the planar structure was previously described, a detailed analysis of its spectroscopic data and biological activity are reported here for the first time. Its absolute configuration was determined using a combination of spectroscopic (¹H–¹H ROESY, ECD, and ...
... A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained ...
... The lichenological history of Saint Lucia is reviewed from published literature and catalogues of herbarium specimens. 238 lichens and 2 lichenicolous fungi are reported. Of these 145 species are known only from single localities in Saint Lucia. Important herbarium collections were made by Alexander Evans, Henry and Frederick Imshaug, Dag Øvstedal, Emmanuël Sérusiaux and the authors. Soufrière is ...
DNA; DNA primers; Phytophthora sojae; amplified fragment length polymorphism; fungi; genes; loci; models; progeny; root rot; soybeans
Abstract:
... The F₂ population derived from a cross between isolates pRₓ (Avr1c-Avr1c) and ps₁ (avr1c-avr1c) of Phytophthora sojae, fungal agent of soybean stem and root rot, was used to determine the genetic basis of avirulence towards Rps1c gene in soybean. The results indicated that this avirulence is dominant and controlled by a single locus, as expected for a simple gene-for-gene model. Segregation of Avr ...
antifungal properties; cell walls; fungi; lectins; mannose; protein secretion
Abstract:
... Ginkbilobin2 is shown to exert antifungal activity through its interaction with a1,2-linked mannose chains of fungal cell wall mannan, which provides clues to the molecular function of the DUF26 protein family . ...
... This work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, which however renders the isolation of th ...
... A solid-state bioprocess with wheat bran and rice husk as substrate was used to isolate filamentous fungi with lipase activity from dairy effluent and soil contaminated with diesel oil. The lipase activity was measured in units, with one unit (U) being defined as the amount of enzyme required to liberate 1 μmol of fatty acids per minute per gram of bran substrate (1 U = 1 μmol min⁻¹ g⁻¹). We obtai ...
... Biology of Termites: A Modern Synthesis (Bignell DE, Roisin Y, Lo N, (Editors), Springer, Dordrecht, 576pp, ISBN 978-90-481-3976-7, e-ISBN 978-90-481-3977-4, DOI 10.1007/978-90-481-3977-4) was published in 2011. With the agreement of the publishers, we give a taxonomic index of the book comprising 494 termite entries, 103 entries of other multicellular animal species mentioned as associates or pre ...
Aspergillus flavus; Ralstonia solanacearum; aflatoxins; bacteria; conidia; fungi; gas chromatography; mass spectrometry; peanuts; plant pathogens; rhizosphere; seeds; virulence
Abstract:
... Microbes in the rhizosphere have a suite of extracellular compounds, both primary and secondary, that communicate with other organisms in their immediate environment. Here, we describe a two-way volatile interaction between two widespread and economically important soil-borne pathogens of peanut, Aspergillus flavus and Ralstonia solanacearum, a fungus and bacterium, respectively. In response to A. ...
... Serine protease inhibitors from bumblebee venom have been shown to block plasmin activity. In this study, we identified the protein BiVSPI from the venom of Bombus ignitus to be a serine protease inhibitor and an antimicrobial factor. BiVSPI is a 55-amino acid mature peptide with ten conserved cysteine residues and a P1 methionine residue. BiVSPI is expressed in the venom gland and also found in t ...
Meyerozyma guilliermondii; case studies; fungi; histology; mares; mycology; pneumonia; uterus
Abstract:
... Ascending infections of equine uterus frequently result in placentitis and abortions; most of these infections are bacterial and are less commonly due to fungi. This report describes an abortion case in an Arab mare due to Candida guilliermondii that was diagnosed via cytological, histological, cultural and biomolecular assays. The histological lesions found were severe necrotizing placentitis ass ...
... The leaves of Cinnamomum burmannii in Guangdong Province were severely affected by two distinctly different types of spots. The dark spot was round, surrounded by a thin yellow zone at the interface between healthy and affected regions, and had a distinct dark ring. The yellow spot had an irregular shape and a broad yellow halo. The pathogens from the different diseased leaves were isolated and we ...
Pinus sylvestris; cell walls; data collection; earlywood; fungi; hardness; hemicellulose; latewood; least squares; lignin; mechanical properties; microstructure; pectins; principal component analysis; sapwood; softwood; structure-activity relationships
Abstract:
... Fungal decay alters the composition, microstructure, and mechanical properties of wood cell walls. To understand better the structure-function relationships during fungal decay, selected annual rings of fungal deteriorated Scots pine sapwood were analyzed in terms of their composition, microstructure, and micromechanical properties. The datasets were acquired separately for earlywood and latewood ...
... Fungi are well known to produce various industrial enzymes and secondary metabolites with different colours. Fungi producing l-asparaginase enzyme are conventionally screened on medium containing phenol red (PR). The contrast between enzyme-hydrolysed zone and unhydrolysed l-asparagine is not very evident and distinct in medium containing PR and bromothymol blue (BB) due to coloured secondary meta ...
... An experiment has been conducted under laboratory conditions to investigate the effect of decomposition of two edible oil cakes, viz. mustard cake (Brassica juncea L) and groundnut cake (Arachis hypogaea L), and two non-edible oil cakes, viz. mahua cake (Madhuca indica Gmel) and neem cake (Azadirachta indica Juss), at the rate of 5.0 t ha⁻¹on the changes of microbial growth and activities in relat ...
... Pentachlorophenol (PCP) is an extremely dangerous worldwide pollutant due to its high toxicity towards all organisms. It has been introduced into the environment mainly as a wood preservative, biocides and from the bleaching of paper or tissues. The use of PCP indiscriminate has led to the contamination of water and soil systems. Many countries have specific regulations, guidelines or procedures f ...