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- Solovchenko, A. E., et al. Show all 5 Authors
- Russian journal of plant physiology 2014 v.61 no.6 pp. 899-909
- DNA; azides; cell cycle; flow cytometry; fluorescence; metabolism; monitoring; phosphorylation; protoplasts; root meristems; thymidine
- ... Beginning with the pioneering work of Salic and Mitchison (2008), the application of thymidine analogue 5-ethynyl-2′-deoxyuridine (EdU) for the detection of cells replicating DNA is actively expanding. Being incorporated into DNA, this nucleoside after click reaction of azide-alkyne cycloaddition with azides of fluorochromes can be easily detected by fluorescence. Recently, protocols of EdU applic ...