Jump to Main Content
- Nakayama, Motokazu, et al. Show all 9 Authors
- Food control 2013 v.33 no.2 pp. 433-439
- Escherichia coli; acids; amino acids; antibacterial properties; epigallocatechin; flavanols; glucose; growth retardation; hydrogen bonding; microorganisms; outer membrane proteins; porins; surface proteins; two-dimensional gel electrophoresis
- ... Catechins have high anti-bacterial activity against various microorganisms. In this study, the mechanism of anti-bacterial activity of catechins was investigated using Escherichia coli. Transmission electron microscope analysis revealed that deposits containing EGCg were found only on the outer membrane, which is the outermost layer of the cell surface, in E. coli cells treated with EGCg. Based on ...
- Nakayama, Motokazu, et al. Show all 10 Authors
- Food control 2013 v.29 no.1 pp. 162-166
- absorbance; bovine serum albumin; catechin; epigallocatechin; equipment; laminin; pH; peroxidase; receptors; temperature; washing
- ... We have developed a method for quantifying gallocatechin gallate (GCg) and epigallocatechin gallate (EGCg) using a catechin-binding peptide (part of the 67-kDa laminin receptor). Using micro titer plates, we investigated various conditions, including the quantifiable range of EGCg concentrations, the optimal concentration of the catechin-binding peptide, and the optimal reaction conditions. In thi ...