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- Tsuji, Shogo, et al. Show all 3 Authors
- Biochemical and biophysical research communications 2013 v.441 pp. 262-265
- DNA; binding sites; evolution; genetic engineering; mutants; nucleotide sequences; point mutation; transcription (genetics); tryptophan
- ... Transcription activator-like effectors (TALEs) are convenient tools for genome engineering at specific genomic sites. However, their use is constrained because most TALE binding sites are preceded by a highly conserved 5′ terminal T nucleotide (5′-T). To remove the 5′-T constraint, we substituted tryptophan 232 in the repeat-1 loop region of the dHax3 N-terminal domain for other amino acids. Furth ...