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You searched for: Publication year rev 7988-2012 Remove constraint Publication year rev: 7988-2012 Publication Year 2012 Remove constraint Publication Year: 2012 Text Availability Full Text Remove constraint Text Availability: Full Text
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1. "Evolution Canyon," a potential microscale monitor of global warming across life

Author:
Eviatar Nevo
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 2960-2965
ISSN:
0027-8424
Subject:
Drosophila; bacteria; biodiversity; drought; extinction; fungi; global warming; heat; mammals; microclimate; models; photosynthesis; prediction; shrublands; solar radiation; sympatric speciation; viruses; Israel
Abstract:
... Climatic change and stress is a major driving force of evolution. The effects of climate change on living organisms have been shown primarily on regional and global scales. Here I propose the "Evolution Canyon" (EC) microscale model as a potential life monitor of global warming in Israel and the rest of the world. The EC model reveals evolution in action at a microscale involving biodiversity dive ...
DOI:
10.1073/pnas.1120633109
PubMed:
22308456
PubMed Central:
PMC3286920

2. "tet(U)" Is Not a Tetracycline Resistance Determinant

Author:
Jamie A. Caryl; Georgina Cox; Stefan Trimble; Alex J. O'Neill
Source:
Antimicrobial agents and chemotherapy 2012 v.56 no.6 pp. 3378-3379
ISSN:
0066-4804
Subject:
antibiotic resistance; bacterial proteins; bioinformatics; genes; plasmids; resistance mechanisms; tetracycline
Abstract:
... The enterococcal plasmid pKQ10 has been reported to carry a poorly characterized tetracycline resistance determinant designated tet(U). However, in a series of studies intended to further characterize this determinant, we have been unable to substantiate the claim that tet(U) confers resistance to tetracyclines. In line with these results, bioinformatic analysis provides compelling evidence that " ...
DOI:
10.1128/AAC.05957-11
PubMed:
22491689
PubMed Central:
PMC3370814

3. (n-3) Fatty Acids and Cardiovascular Health: Are Effects of EPA and DHA Shared or Complementary?

Author:
Dariush Mozaffarian; Jason H. Y. Wu
Source:
Journal of nutrition 2012 v.142 no.3 pp. 614S-625S
ISSN:
0022-3166
Subject:
docosapentaenoic acid; high density lipoprotein; fish consumption; humans; compliance; inflammation; laboratory animals; endogenous sources; particle size; platelet aggregation; low density lipoprotein; risk factors; animal models; arrhythmia; fish oils; clinical trials; oxidative stress; research support
Abstract:
... Considerable research supports cardiovascular benefits of consuming omega-3 PUFA, also known as (n-3) PUFA, from fish or fish oil. Whether individual long-chain (n-3) PUFA have shared or complementary effects is not well established. We reviewed evidence for dietary and endogenous sources and cardiovascular effects on biologic pathways, physiologic risk factors, and clinical endpoints of EPA [20:5 ...
DOI:
10.3945/jn.111.149633
PubMed:
22279134
PubMed Central:
PMC3278271

4. (φ,ψ)₂ Motifs: A Purely Conformation-Based Fine-Grained Enumeration of Protein Parts at the Two-Residue Level

Author:
Scott A. Hollingsworth; Matthew C. Lewis; Donald S. Berkholz; Weng-Keen Wong; P. Andrew Karplus
Source:
Journal of molecular biology 2012 v.416 no.1 pp. 78-93
ISSN:
0022-2836
Subject:
algorithms; models; polypeptides; population density; protein structure; proteins
Abstract:
... A deep understanding of protein structure benefits from the use of a variety of classification strategies that enhance our ability to effectively describe local patterns of conformation. Here, we use a clustering algorithm to analyze 76,533 all-trans segments from protein structures solved at 1.2 Å resolution or better to create a purely φ,ψ-based comprehensive empirical categorization of common c ...
DOI:
10.1016/j.jmb.2011.12.022
PubMed:
22198294
PubMed Central:
PMC3268948

5. (−)-Epicatechin prevents TNFα-induced activation of signaling cascades involved in inflammation and insulin sensitivity in 3T3-L1 adipocytes

Author:
Marcela A. Vazquez-Prieto; Ahmed Bettaieb; Fawaz G. Haj; César G. Fraga; Patricia I. Oteiza
Source:
Archives of biochemistry and biophysics 2012 v.527 no.2 pp. 113-118
ISSN:
0003-9861
Subject:
adipocytes; adipose tissue; chronic diseases; extracts; genes; inflammation; insulin; insulin resistance; interleukin-6; metabolic syndrome; mitogen-activated protein kinase; noninsulin-dependent diabetes mellitus; nuclear genome; obesity; phosphorylation; public health; resistin; risk; transcription factor NF-kappa B; transcriptional activation; tumor necrosis factor-alpha
Abstract:
... Obesity is major public health concern worldwide and obese individuals exhibit a higher risk of chronic diseases such as type 2 diabetes. Inflammation plays a significant role in metabolic regulation and mounting evidence highlight the contribution of adipose tissue to systemic inflammatory state. Food extracts with a high content of (−)-epicatechin have been found to exert systemic anti-inflammat ...
DOI:
10.1016/j.abb.2012.02.019
PubMed:
22425757
PubMed Central:
PMC3992864

6. L: -Theanine extends lifespan of adult Caenorhabditis elegans

Author:
Kim Zarse; Saskia Jabin; Michael Ristow
Source:
European journal of nutrition 2012 v.51 no.6 pp. 765-768
ISSN:
1436-6207
Subject:
Caenorhabditis elegans; Camellia sinensis; Escherichia coli; adults; agar; bacteria; blood; green tea; humans; longevity; medicine; paraquat
Abstract:
... PURPOSE: Compounds that delay aging in model organisms may be of significant interest to anti-aging medicine, since these substances potentially provide pharmaceutical approaches to promote healthy lifespan in humans. We here aimed to test whether pharmaceutical concentrations of L: -theanine, a putative anti-cancer, anti-obesity, blood pressure-lowering, and neuroprotective compound contained in ...
DOI:
10.1007/s00394-012-0341-5
PubMed:
22422488
PubMed Central:
PMC3419347

7. 1,2,3-Triazole–Heme Interactions in Cytochrome P450: Functionally Competent Triazole–Water–Heme Complexes

Author:
Conner Kip P.; Vennam Preethi; Woods Caleb M.; Krzyaniak Matthew D.; Bowman Michael K.; Atkins William M.
Source:
Biochemistry 2012 v.51 no.32 pp. 6441-6457
ISSN:
1520-4995
Subject:
cytochrome P-450; electron paramagnetic resonance spectroscopy; enthalpy; entropy; heme; heme iron; hydrogen bonding; ligands; metabolism; models; risk
Abstract:
... In comparison to imidazole (IMZ) and 1,2,4-triazole (1,2,4-TRZ), the isosteric 1,2,3-triazole (1,2,3-TRZ) is unrepresented among cytochrome P450 (CYP) inhibitors. This is surprising because 1,2,3-TRZs are easily obtained via “click” chemistry. To understand this underrepresentation of 1,2,3-TRZs among CYP inhibitors, thermodynamic and density functional theory computational studies were performed ...
DOI:
10.1021/bi300744z
PubMed:
22809252
PubMed Central:
PMC3448000

8. The 1,2-Benzenedithiole-Based Cyclocondensation Assay: A Valuable Tool for the Measurement of Chemopreventive Isothiocyanates

Author:
Yuesheng Zhang
Source:
Critical reviews in food science and nutrition 2012 v.52 no.6 pp. 525-532
ISSN:
1549-7852
Subject:
chemoprevention; humans; isothiocyanates; mechanism of action; metabolites; vegetables
Abstract:
... Many naturally occurring isothiocyanates (ITCs) show highly promising chemopreventive activities. Humans are commonly exposed to these compounds through the consumption of cruciferous vegetables which are the main source of dietary ITCs. Dietary ITCs may play an important role in cancer prevention and in the well-recognized cancer preventive activities of cruciferous vegetables. A generic analytic ...
DOI:
10.1080/10408398.2010.503288
PubMed:
22452732
PubMed Central:
PMC3315191

9. 1,2-dielaidoylphosphocholine/1,2-dimyristoylphosphoglycerol supported phospholipid bilayer formation in calcium and calcium-free buffer

Author:
Evans, Kervin O.
Source:
Thin solid films 2012 v.520 no.7 pp. 3026-3030
ISSN:
0040-6090
Subject:
atomic force microscopy; buffers; calcium; ions; lipid bilayers; lipid composition; monitoring; phosphatidylcholines; phosphatidylglycerols
Abstract:
... Phospholipid membranes are useful in the field of biocatalysis because a supported phospholipid membrane can create a biomimetic platform where biocatalytic processes can readily occur. In this work, supported bilayer formation from sonicated phospholipid vesicles containing 1,2-dielaidoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] was studied using a q ...
Handle:
10113/55125
DOI:
10.1016/j.tsf.2011.12.002

10. 1,4-Diazaspiro[2.2]pentanes as a Flexible Platform for the Synthesis of Diamine-Bearing Stereotriads

Author:
Rigoli Jared W.; Boralsky Luke A.; Hershberger John C.; Marston Dagmara; Meis Alan R.; Guzei Ilia A.; Schomaker Jennifer M.
Source:
Journal of organic chemistry 2012 v.77 no.5 pp. 2446-2455
ISSN:
1520-6904
Subject:
Lewis bases; alcohols; allene; aziridines; bioactive compounds; chemical structure; optical isomerism; organic chemistry; oxidation; regioselectivity
Abstract:
... Nitrogen-containing stereotriads occur in a number of biologically active compounds, but general and flexible methods to access these compounds are limited mainly to the manipulation of chiral olefins. An alternative approach is to employ a highly chemo-, regio-, and stereocontrolled allene oxidation that can install a new carbon–heteroatom bond at each of the three original allene carbons. In thi ...
DOI:
10.1021/jo3000282
PubMed:
22304460
PubMed Central:
PMC4500119

11. 1,500 year quantitative reconstruction of winter precipitation in the Pacific Northwest

Author:
Byron A. Steinman; Mark B. Abbott; Michael E. Mann; Nathan D. Stansell; Bruce P. Finney
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.29 pp. 11619-11623
ISSN:
0027-8424
Subject:
El Nino; climate; drought; growth rings; isotopes; lakes; oxygen; paleoclimatology; seasonal variation; sediments; winter
Abstract:
... Multiple paleoclimate proxies are required for robust assessment of past hydroclimatic conditions. Currently, estimates of drought variability over the past several thousand years are based largely on tree-ring records. We produced a 1,500-y record of winter precipitation in the Pacific Northwest using a physical model-based analysis of lake sediment oxygen isotope data. Our results indicate that ...
DOI:
10.1073/pnas.1201083109
PubMed:
22753510
PubMed Central:
PMC3406856

12. 1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

Author:
Warlick Benjamin P.; Evans Bradley S.; Erb Tobias J.; Ramagopal Udipi A.; Sriram Jaya; Imker Heidi J.; Sauder J. Michael; Bonanno Jeffrey B.; Burley Stephen K.; Tabita F. Robert; Almo Steven C.; Sweedler Jonathan S.; Gerlt John A.
Source:
Biochemistry 2012 v.51 no.42 pp. 8324-8326
ISSN:
1520-4995
Subject:
Rhodospirillum rubrum; S-adenosylmethionine; active sites; adenine; biosynthesis; dimethyl disulfide; isomerization; isoprenoids; polyamines
Abstract:
... Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosph ...
DOI:
10.1021/bi301215g
PubMed:
23035785
PubMed Central:
PMC3490199

13. The 10-Year Cost-Effectiveness of Lifestyle Intervention or Metformin for Diabetes Prevention: An intent-to-treat analysis of the DPP/DPPOS

Author:
Source:
Diabetes care 2012 v.35 no.4 pp. 723-730
ISSN:
0149-5992
Subject:
adults; cost effectiveness; lifestyle; metformin; noninsulin-dependent diabetes mellitus; quality of life; quality-adjusted life year
Abstract:
... OBJECTIVE: The Diabetes Prevention Program (DPP) and its Outcomes Study (DPPOS) demonstrated that either intensive lifestyle intervention or metformin could prevent type 2 diabetes in high-risk adults for at least 10 years after randomization. We report the 10-year within-trial cost-effectiveness of the interventions. RESEARCH DESIGN AND METHODS: Data on resource utilization, cost, and quality of ...
DOI:
10.2337/dc11-1468
PubMed:
22442395
PubMed Central:
PMC3308273

14. 100% Orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003-2006

Author:
Carol E O’Neil; Theresa A Nicklas; Gail C Rampersaud; Victor L Fulgoni III
Source:
Nutrition journal 2012 v.11 no.1 pp. 565
ISSN:
1475-2891
Subject:
Adequate Intakes; food groups; odds ratio; folic acid; nutrients; adults; Estimated Average Requirement; biomarkers; orange juice; metabolic syndrome; risk factors; potassium; childhood obesity; USDA; body mass index; vitamin A; ascorbic acid; fruits; nutritional adequacy; regression analysis; magnesium; cholesterol; healthy diet; fruit consumption; National Health and Nutrition Examination Survey; whole grain foods
Abstract:
... BACKGROUND: Consumption of 100% orange juice (OJ) has been positively associated with nutrient adequacy and diet quality, with no increased risk of overweight/obesity in children; however, no one has examined these factors in adults. The purpose of this study was to examine the association of 100% OJ consumption with nutrient adequacy, diet quality, and risk factors for metabolic syndrome (MetS) i ...
DOI:
10.1186/1475-2891-11-107
PubMed:
23234248
PubMed Central:
PMC3545988

15. IL- 10–induced microRNA-187 negatively regulates TNF-α, IL-6, and IL-12p40 production in TLR4-stimulated monocytes

Author:
Marzia Rossato; Graziella Curtale; Nicola Tamassia; Monica Castellucci; Laura Mori; Sara Gasperini; Barbara Mariotti; Mariacristina De Luca; Massimiliano Mirolo; Marco A. Cassatella; Massimo Locati; Flavia Bazzoni
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.45 pp. E3101
ISSN:
0027-8424
Subject:
Toll-like receptor 4; humans; interleukin-10; interleukin-12; interleukin-6; messenger RNA; microRNA; monocytes; physiological regulation; transcription (genetics); transcription factors; translation (genetics); tumor necrosis factor-alpha
Abstract:
... IL-10 is a potent anti-inflammatory molecule that, in phagocytes, negatively targets cytokine expression at transcriptional and posttranscriptional levels. Posttranscriptional checkpoints also represent the specific target of a recently discovered, evolutionary conserved class of small silencing RNAs known as “microRNAs” (miRNAs), which display the peculiar function of negatively regulating mRNA p ...
DOI:
10.1073/pnas.1209100109
PubMed:
23071313
PubMed Central:
PMC3494907

16. A 12-week double-blind randomized clinical trial of vitamin D₃ supplementation on body fat mass in healthy overweight and obese women

Author:
Amin Salehpour; Farhad Hosseinpanah; Farzad Shidfar; Mohammadreza Vafa; Maryam Razaghi; Sahar Dehghani; Anahita Hoshiarrad; Mahmoodreza Gohari
Source:
Nutrition journal 2012 v.11 no.1 pp. 553
ISSN:
1475-2891
Subject:
body fat; body mass index; cholecalciferol; clinical trials; lactose; obesity; placebos; waist; waist circumference; women
Abstract:
... BACKGROUND: Vitamin D concentrations are linked to body composition indices, particularly body fat mass. Relationships between hypovitaminosis D and obesity, described by both BMI and waist circumference, have been mentioned. We have investigated the effect of a 12-week vitamin D3 supplementation on anthropometric indices in healthy overweight and obese women. METHODS: In a double-blind, randomize ...
DOI:
10.1186/1475-2891-11-78
PubMed:
22998754
PubMed Central:
PMC3514135

17. 14-3-3 Proteins SGF14c and SGF14l Play Critical Roles during Soybean Nodulation

Author:
Osman Radwan; Xia Wu; Manjula Govindarajulu; Marc Libault; David J. Neece; Man-Ho Oh; R. Howard Berg; Gary Stacey; Christopher G. Taylor; Steven C. Huber; Steven J. Clough
Source:
Plant physiology 2012 v.160 no.4 pp. 2125-2136
ISSN:
0032-0889
Subject:
Bradyrhizobium japonicum; Glycine max; RNA interference; Rhizobium rhizogenes; cytoplasm; developmental stages; early development; genes; messenger RNA; nodulation; phenotype; proteins; proteomics; reverse transcriptase polymerase chain reaction; roots; soybeans; transcription (genetics); transcriptomics; transmission electron microscopy
Abstract:
... The soybean (Glycine max) genome contains 18 members of the 14-3-3 protein family, but little is known about their association with specific phenotypes. Here, we report that the Glyma0529080 Soybean G-box Factor 14-3-3c (SGF14c) and Glyma08g12220 (SGF14l) genes, encoding 14-3-3 proteins, appear to play essential roles in soybean nodulation. Quantitative reverse transcription-polymerase chain react ...
DOI:
10.1104/pp.112.207027
PubMed:
23060368
PubMed Central:
PMC3510136

18. 14-3-3 fusion oncogenes in high-grade endometrial stromal sarcoma

Author:
Cheng-Han Lee; Wen-Bin Ou; Adrian Mariño-Enriquez; Meijun Zhu; Mark Mayeda; Yuexiang Wang; Xiangqian Guo; Alayne L. Brunner; Frédéric Amant; Christopher A. French; Robert B. West; Jessica N. McAlpine; C. Blake Gilks; Michael B. Yaffe; Leah M. Prentice; Andrew McPherson; Steven J. M. Jones; Marco A. Marra; Sohrab P. Shah; Matt van de Rijn; David G. Huntsman; Paola Dal Cin; Maria Debiec-Rychter; Marisa R. Nucci; Jonathan A. Fletcher
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.3 pp. 929-934
ISSN:
0027-8424
Subject:
cell growth; cytogenetics; disease course; exons; fluorescence in situ hybridization; gene expression; gene fusion; nuclear localization signals; oncogenes; protein synthesis; proteins; sarcoma; sequence analysis; small interfering RNA
Abstract:
... 14-3-3 proteins are ubiquitously expressed regulators of various cellular functions, including proliferation, metabolism, and differentiation, and altered 14-3-3 expression is associated with development and progression of cancer. We report a transforming 14-3-3 oncoprotein, which we identified through conventional cytogenetics and whole-transcriptome sequencing analysis as a highly recurrent gene ...
DOI:
10.1073/pnas.1115528109
PubMed:
22223660
PubMed Central:
PMC3271913

19. 14-3-3 proteins act as scaffolds for GmMYB62 and GmMYB176 and regulate their intracellular localization in soybean

Author:
Xuyan Li; Sangeeta Dhaubhadel
Source:
Plant signaling & behavior 2012 v.7 no.8 pp. 965-968
ISSN:
1559-2324
Subject:
Fabaceae; biosynthesis; clinical trials; food intake; gene expression; human health; isoflavonoids; nitrogen fixation; proteins; soybeans; stress tolerance
Abstract:
... Isoflavonoids are plant natural compounds predominantly found in leguminous plant. They play important functions in both nitrogen fixation and stress resistance. Many clinical studies have linked dietary intake of isoflavonoids to human health benefits. Binding of 14-3-3 proteins to GmMYB176, an isoflavonoid regulator, modulates expression of key isoflavonoids gene expression and its biosynthesis. ...
DOI:
10.4161/psb.20940
PubMed:
22836494
PubMed Central:
PMC3474696

20. The 14-3-3 proteins of Arabidopsis regulate root growth and chloroplast development as components of the photosensory system

Author:
John D. Mayfield; Anna-Lisa Paul; Robert J. Ferl
Source:
Journal of experimental botany 2012 v.63 no.8 pp. 3061-3070
ISSN:
0022-0957
Subject:
Arabidopsis; growth and development; light intensity; mutants; protein binding; proteins; red light; root growth; roots; transfer DNA
Abstract:
... The 14-3-3 proteins specifically bind a number of client proteins to influence important pathways, including flowering timing via the photosensory system. For instance, 14-3-3 proteins influence the photosensory system through interactions with Constans (CO) protein. 14-3-3 associations with the photosensory system were further studied in this investigation using 14-3-3 T-DNA insertion mutants to ...
DOI:
10.1093/jxb/ers022
PubMed:
22378945
PubMed Central:
PMC3350920

21. 15N-Labeled Brain Enables Quantification of Proteome and Phosphoproteome in Cultured Primary Neurons

Author:
Liao Lujian; Sando Richard C.; Farnum John B.; Vanderklish Peter W.; Maximov Anton; Yates John R.
Source:
Journal of Proteome Research 2012 v.11 no.2 pp. 1341-1353
ISSN:
1535-3907
Subject:
brain; models; neurons; phosphopeptides; phosphorylation; proteins; proteome; proteomics; quantitative analysis; stable isotopes; synapse; synaptic transmission
Abstract:
... Terminally differentiated primary cells represent a valuable in vitro model to study signaling events associated within a specific tissue. Quantitative proteomic methods using metabolic labeling in primary cells encounter labeling efficiency issues hindering the use of these cells. Here we developed a method to quantify the proteome and phosphoproteome of cultured neurons using ¹⁵N-labeled brain t ...
DOI:
10.1021/pr200987h
PubMed:
22070516
PubMed Central:
PMC3279198

22. 15N-Labeled Full-Length Apolipoprotein E4 as an Internal Standard for Mass Spectrometry Quantification of Apolipoprotein E Isoforms

Author:
Wang Meiyao; Chen Junjun; Turko Illarion V.
Source:
Analytical chemistry 2012 v.84 no.19 pp. 8340-8344
ISSN:
1520-6882
Subject:
Alzheimer disease; alleles; amino acids; apolipoprotein E4; brain; genotype; humans; mass spectrometry; quantitative analysis; risk factors
Abstract:
... Apolipoprotein E isoforms (apoE2, apoE3, and apoE4) affect the likelihood of developing Alzheimer’s disease (AD), with the apoE4 isoform being a major risk factor. However, the underlying mechanism remains to be determined. ApoE isoforms vary by a single amino acid change, and it is a challenge to distinguish them on the protein level. We developed a mass spectrometry-based quantitative method uti ...
DOI:
10.1021/ac3018873
PubMed:
22931427
PubMed Central:
PMC4038910

23. 17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)

Author:
T. S. Daly-Engel; R. L. Smith; D. S. Finn; M. E. Knoderbane; I. C. Phillipsen; D. A. Lytle
Source:
Conservation genetics resources 2012 v.4 no.4 pp. 979-981
ISSN:
1877-7252
Subject:
Lethocerus americanus; alleles; aquatic habitat; climate change; evolution; groundwater; insects; loci; mating systems; microsatellite repeats
Abstract:
... The giant water bug (Abedus herberti) is a large flightless insect that is a keystone predator in aridland aquatic habitats. Extended droughts, possibly due to climate change and groundwater pumping, are causing once-perennial aquatic habitats to dry, resulting in serious conservation concern for some populations. A. herberti also exhibits exclusive male parental care, which has made it a model or ...
DOI:
10.1007/s12686-012-9687-5
PubMed:
24077753
PubMed Central:
PMC3784353

24. 1H Dynamic Nuclear Polarization Based on an Endogenous Radical

Author:
Maly Thorsten; Cui Dongtao; Griffin Robert G.; Miller Anne-Frances
Source:
Journal of physical chemistry 2012 v.116 no.24 pp. 7055-7065
ISSN:
1520-6106
Subject:
flavins; free radicals; nuclear magnetic resonance spectroscopy; organelles; physical chemistry; proteins
Abstract:
... We demonstrate a 15-fold enhancement of solid-state NMR signals via dynamic nuclear polarization (DNP) based on a stable, naturally occurring radical in a protein: the flavin mononucleotide (FMN) semiquinone of flavodoxin. The line width of flavodoxin’s EPR signal suggests that the dominant DNP mechanism is the solid effect, consistent with the field-dependent DNP enhancement profile. The magnitud ...
DOI:
10.1021/jp300539j
PubMed:
22472179
PubMed Central:
PMC6010065

25. 1α,25-Dihydroxyvitamin D₃ and its analog, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D₃ (2MD), suppress intraocular pressure in non-human primates

Author:
Galina D. Kutuzova; B’Ann T. Gabelt; Julie A. Kiland; Elizabeth A. Hennes-Beann; Paul L. Kaufman; Hector F. DeLuca
Source:
Archives of biochemistry and biophysics 2012 v.518 no.1 pp. 53-60
ISSN:
0003-9861
Subject:
Primates; drainage; genes; glaucoma; hypertension; microarray technology; people; risk factors; therapeutics
Abstract:
... Ocular hypertension is the greatest known risk factor for glaucoma that affects an estimated 70million people worldwide. Lowering intraocular pressure (IOP) remains the mainstay of therapy in the management of glaucoma. By means of microarray analysis, we have discovered that 1α,25-dihydroxyvitamin D₃ (1α,25-(OH)₂D₃) regulates genes that are known to be involved in the determination of intraocular ...
DOI:
10.1016/j.abb.2011.10.022
PubMed:
22198282
PubMed Central:
PMC3390938

26. 1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

Author:
Lionel LA In; Norhafiza M Arshad; Halijah Ibrahim; Mohamad Nurul Azmi; Khalijah Awang; Noor Hasima Nagoor
Source:
BMC complementary and alternative medicine 2012 v.12 no.1 pp. 179
ISSN:
1472-6882
Subject:
DNA fragmentation; Western blotting; acetates; alternative medicine; apoptosis; biomarkers; body weight changes; cisplatin; clinical trials; cyclins; cytotoxicity; genes; ginger; humans; mechanism of action; mice; mouth neoplasms; squamous cell carcinoma; survival rate; transcription factor NF-kappa B
Abstract:
... BACKGROUND: Oral cancers although preventable, possess a low five-year survival rate which has remained unchanged over the past three decades. In an attempt to find a more safe, affordable and effective treatment option, we describe here the use of 1’S-1’-acetoxychavicol acetate (ACA), a component of Malaysian ginger traditionally used for various medicinal purposes. METHODS: Whether ACA can inhib ...
DOI:
10.1186/1472-6882-12-179
PubMed:
23043547
PubMed Central:
PMC3517351

27. 2,2,2-Trifluoroethyl 6-thio-β-d-glucopyranoside as a selective tag for cysteines in proteins

Author:
Richard F.G. Fröhlich; Evelyne Schrank; Klaus Zangger
Source:
Carbohydrate research 2012 v.361 no. pp. 100-104
ISSN:
0008-6215
Subject:
cysteine; fluorine; glucose; glycosylation; microwave treatment; nuclear magnetic resonance spectroscopy; risk; solvents; thiols; transactivators
Abstract:
... A synthetic route to a trifluoromethyl and thiol containing glucose derivative (2,2,2-trifluoroethyl 6-thio-β-d-glucopyranoside) is presented, which is based on microwave-assisted Fischer glycosylation under increased pressure. This water-soluble, neutral thiol-compound can be used to selectively introduce a fluorine probe for ¹⁹F NMR spectroscopy on cysteines in proteins. It can be attached under ...
DOI:
10.1016/j.carres.2012.08.010
PubMed:
23000216
PubMed Central:
PMC4067056

28. 2,2′,3,5′,6-Pentachlorobiphenyl (PCB 95) and Its Hydroxylated Metabolites Are Enantiomerically Enriched in Female Mice

Author:
Kania-Korwel Izabela; Barnhart Christopher D.; Stamou Marianna; Truong Kim M.; El-Komy Mohammed H. M. E.; Lein Pamela J.; Veng-Pedersen Peter; Lehmler Hans-Joachim
Source:
Environmental Science & Technology 2012 v.46 no.20 pp. 11393-11401
ISSN:
1520-5851
Subject:
adults; blood; brain; dose response; females; liver; metabolites; mice; neurotoxicity; polychlorinated biphenyls; subchronic exposure; tissues
Abstract:
... Epidemiological and laboratory studies link polychlorinated biphenyls and their metabolites to adverse neurodevelopmental outcomes. Several neurotoxic PCB congeners are chiral and undergo enantiomeric enrichment in mammalian species, which may modulate PCB developmental neurotoxicity. This study measures levels and enantiomeric enrichment of PCB 95 and its hydroxylated metabolites (OH-PCBs) in adu ...
DOI:
10.1021/es302810t
PubMed:
22974126
PubMed Central:
PMC3475189

29. 2,3-Hexanediols as Sex Attractants and a Female-produced Sex Pheromone for Cerambycid Beetles in the Prionine Genus Tragosoma

Author:
Ann M. Ray; James D. Barbour; J. Steven McElfresh; Jardel A. Moreira; Ian Swift; Ian M. Wright; Alenka Žunič; Robert F. Mitchell; Elizabeth E. Graham; Ronald L. Alten; Jocelyn G. Millar; Lawrence M. Hanks
Source:
Journal of chemical ecology 2012 v.38 no.9 pp. 1151-1158
ISSN:
0098-0331
Subject:
Cerambycidae; aggregation pheromones; bioassays; females; fresh produce; males; pheromone blends; pheromone glands; sex attractants; sex pheromones; sexual dimorphism; California
Abstract:
... Recent work suggests that closely related cerambycid species often share pheromone components, or even produce pheromone blends of identical composition. However, little is known of the pheromones of species in the subfamily Prioninae. During field bioassays in California, males of three species in the prionine genus Tragosoma were attracted to 2,3-hexanediols, common components of male-produced a ...
DOI:
10.1007/s10886-012-0181-z
PubMed:
22923142
PubMed Central:
PMC3808253

30. 2-Hydroxyoleate, a nontoxic membrane binding anticancer drug, induces glioma cell differentiation and autophagy

Author:
Silvia Terés; Victoria Lladó; Mónica Higuera; Gwendolyn Barceló-Coblijn; Maria Laura Martin; Maria Antònia Noguera-Salvà; Amaia Marcilla-Etxenike; José Manuel García-Verdugo; Mario Soriano-Navarro; Carlos Saus; Ulises Gómez-Pinedo; Xavier Busquets; Pablo V. Escribá
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.22 pp. 8489-8494
ISSN:
0027-8424
Subject:
anticarcinogenic activity; antineoplastic agents; brain; cell death; cell differentiation; cytoplasm; lipid composition; mechanism of action; mitogen-activated protein kinase; neoplasms; neuroglia; oral administration; patients; plasma membrane; protein phosphorylation; protein transport; proteins; relapse; sphingomyelins; survival rate; toxicity
Abstract:
... Despite recent advances in the development of new cancer therapies, the treatment options for glioma remain limited, and the survival rate of patients has changed little over the past three decades. Here, we show that 2-hydroxyoleic acid (2OHOA) induces differentiation and autophagy of human glioma cells. Compared to the current reference drug for this condition, temozolomide (TMZ), 2OHOA combated ...
DOI:
10.1073/pnas.1118349109
PubMed:
22586083
PubMed Central:
PMC3365159

31. 2-Methoxyestradiol inhibits experimental autoimmune encephalomyelitis through suppression of immune cell activation

Author:
Gordon S. Duncan; Dirk Brenner; Michael W. Tusche; Anne Brüstle; Christiane B. Knobbe; Andrew J. Elia; Thomas Mock; Mark R. Bray; Peter H. Krammer; Tak W. Mak
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.51 pp. 21034-21039
ISSN:
0027-8424
Subject:
T-lymphocytes; animal models; cytokines; dose response; drugs; encephalitis; estradiol; humans; lymphocyte proliferation; metabolites; mice; neoplasms; rheumatoid arthritis; sclerosis; transcription (genetics); transcription factor NF-kappa B
Abstract:
... The endogenous metabolite of estradiol, 2-Methoxyestradiol (2ME2), is an antimitotic and antiangiogenic cancer drug candidate that also exhibits disease-modifying activity in animal models of rheumatoid arthritis (RA). We found that 2ME2 dramatically suppresses development of mouse experimental autoimmune encephalomyelitis (EAE), a rodent model of multiple sclerosis (MS). 2ME2 inhibits in vitro ly ...
DOI:
10.1073/pnas.1215558110
PubMed:
23213242
PubMed Central:
PMC3529073

32. The 2009 pandemic H1N1 virus induces anti-neuraminidase (NA) antibodies that cross-react with the NA of H5N1 viruses in ferrets

Author:
Zhongying Chen; Lomi Kim; Kanta Subbarao; Hong Jin
Source:
Vaccine 2012 v.30 no.15 pp. 2516-2522
ISSN:
0264-410X
Subject:
Influenza A virus; amino acids; antibodies; birds; cross reaction; ferrets; humans; influenza; pandemic; proteins; sialidase; vaccines; viruses
Abstract:
... A miniaturized neuraminidase inhibition (NI) assay using HA-mismatched H6 reassortant viruses was performed to examine the neuraminidase (NA)-specific antibody response in ferrets immunized with live-attenuated influenza vaccine (LAIV) strains. The strains tested possessed different NAs derived from seasonal H1N1 and H3N2, 2009 pandemic H1N1, and the highly pathogenic influenza H5N1 virus. The ant ...
DOI:
10.1016/j.vaccine.2012.01.090
PubMed:
22330124
PubMed Central:
PMC4792253

33. A 21-day Daniel fast with or without krill oil supplementation improves anthropometric parameters and the cardiometabolic profile in men and women

Author:
John F Trepanowski; Mohammad M Kabir; Rick J Alleman Jr; Richard J Bloomer
Source:
Nutrition & metabolism 2012 v.9 no.1 pp. 478
ISSN:
1743-7075
Subject:
alcohols; animal products; blood; blood glucose; blood lipids; blood pressure; body mass index; body weight; caffeine; coconut oil; flavorings; flour; glucose; insulin; insulin resistance; krill; men; oxidative stress; placebos; preservatives; sweeteners; women
Abstract:
... BACKGROUND: The Daniel Fast is a vegan diet that prohibits the consumption of animal products, refined foods, white flour, preservatives, additives, sweeteners, flavorings, caffeine, and alcohol. Following this dietary plan for 21 days has been demonstrated to improve blood pressure, LDL-C, and certain markers of oxidative stress, but it has also been shown to lower HDL-C. Krill oil supplementatio ...
DOI:
10.1186/1743-7075-9-82
PubMed:
22971786
PubMed Central:
PMC3517900

34. The 21.5-kDa isoform of myelin basic protein has a non-traditional PY-nuclear-localization signal

Author:
Graham S.T. Smith; Lauren V. Seymour; Joan M. Boggs; George Harauz
Source:
Biochemical and biophysical research communications 2012 v.422 no.4 pp. 670-675
ISSN:
0006-291X
Subject:
actin; arginine; central nervous system; fluorescence microscopy; fluorescent proteins; genes; lysine; myelin basic protein; myelin sheath; protein transport; site-directed mutagenesis; tubulin
Abstract:
... The predominant 18.5-kDa classic myelin basic protein (MBP) is mainly responsible for compaction of the myelin sheath in the central nervous system, but is multifunctional, having numerous interactions with Ca²⁺-calmodulin, actin, tubulin, and SH3-domains, and can tether these proteins to a lipid membrane in vitro. The full-length 21.5-kDa MBP isoform has an additional 26 residues encoded by exon- ...
DOI:
10.1016/j.bbrc.2012.05.051
PubMed:
22609403
PubMed Central:
PMC3526657

35. 24-Hour profiles of circulating ghrelin and peptide YY are inversely associated in normal weight premenopausal women

Author:
Brenna R. Hill; Mary Jane De Souza; David A. Wagstaff; Rino Sato; Nancy I. Williams
Source:
Peptides 2012 v.38 no.1 pp. 159-162
ISSN:
0196-9781
Subject:
blood; body fat; body mass index; carbohydrates; food intake; ghrelin; humans; hypothalamus; models; peptide YY; premenopause; radioimmunoassays; secretion; statistical analysis; women
Abstract:
... Peptide YY (PYY) and ghrelin (GHR) may modulate one another's actions within the hypothalamus. Peripheral infusion of PYY in humans acutely suppresses circulating concentrations of GHR. Whether an association between PYY and GHR exists in the peripheral circulation of humans over 24h is unknown. The purpose of this study was to determine if circulating concentrations of PYY and GHR were significan ...
DOI:
10.1016/j.peptides.2012.08.017
PubMed:
22954902
PubMed Central:
PMC3496067

36. 25-Hydroxyvitamin D Concentrations and In Vivo Insulin Sensitivity and β-Cell Function Relative to Insulin Sensitivity in Black and White Youth

Author:
Kumaravel Rajakumar; Javier de las Heras; SoJung Lee; Michael F. Holick; Silva A. Arslanian
Source:
Diabetes care 2012 v.35 no.3 pp. 627-633
ISSN:
0149-5992
Subject:
Blacks; Whites; adipose tissue; adiposity; body mass index; body measurements; fasting; glucose; homeostasis; insulin; insulin resistance; secretion; vitamin D; volunteers; youth
Abstract:
... OBJECTIVE: To examine the relationships between plasma 25-hydroxyvitamin D [25(OH)D] and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity, disposition index (DI), in black and white youth. RESEARCH DESIGN AND METHODS: Plasma 25(OH)D concentrations were analyzed in banked specimens in healthy youth aged 8 to 18 years who had existing data on hyperinsulinemic-euglycemi ...
DOI:
10.2337/dc11-1825
PubMed:
22238280
PubMed Central:
PMC3322701

37. 25-Hydroxyvitamin D deficiency is associated with fatal stroke among whites but not blacks: The NHANES-III linked mortality files

Author:
Erin D. Michos; Jared P. Reis; Wendy S. Post; Pamela L. Lutsey; Rebecca F. Gottesman; Thomas H. Mosley; A. Richey Sharrett; Michal L. Melamed
Source:
Nutrition 2012 v.28 no.4 pp. 367-371
ISSN:
0899-9007
Subject:
risk factors; regression analysis; confidence interval; socioeconomic status; Whites; stroke; vitamin deficiencies; Blacks; National Health and Nutrition Examination Survey; mortality; adults; United States
Abstract:
... OBJECTIVE: Deficient 25-hydroxyvitamin D (25[OH]D) levels are associated with cardiovascular disease (CVD) events and mortality. 25(OH)D deficiency and stroke are more prevalent in blacks. We examined whether low 25(OH)D contributes to the excess risk of fatal stroke in blacks compared with whites. METHODS: The Third National Health and Nutrition Examination Survey, a probability sample of U.S. ci ...
DOI:
10.1016/j.nut.2011.10.015
PubMed:
22261577
PubMed Central:
PMC3304002

38. 26S Proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells

Author:
Susan E. Samaras; Billy Chen; Stephen R. Koch; Douglas B. Sawyer; Chee Chew Lim; Jeffrey M. Davidson
Source:
Biochemical and biophysical research communications 2012 v.425 no.4 pp. 830-835
ISSN:
0006-291X
Subject:
proteasome endopeptidase complex; messenger RNA; tissue repair; endothelial cells; humans; muscles; sarcomeres; proteolysis; rats; adults; half life
Abstract:
... Ankyrin repeat domain 1 protein (Ankrd1), also known as cardiac ankyrin repeat protein (CARP), increases dramatically after tissue injury, and its overexpression improves aspects of wound healing. Reports that Ankrd1/CARP protein stability may affect cardiovascular organization, together with our findings that the protein is crucial to stability of the cardiomyocyte sarcomere and increased in woun ...
DOI:
10.1016/j.bbrc.2012.07.162
PubMed:
22892129
PubMed Central:
PMC3460693

39. The 27–year decline of coral cover on the Great Barrier Reef and its causes

Author:
Glenn De’ath; Katharina E. Fabricius; Hugh Sweatman; Marji Puotinen
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.44 pp. 17995-17999
ISSN:
0027-8424
Subject:
Acanthaster planci; bleaching; climatic factors; control methods; coral bleaching; coral reefs; corals; hurricanes; mortality; predation; surveys; temporal variation; time series analysis; water quality; Great Barrier Reef
Abstract:
... The world’s coral reefs are being degraded, and the need to reduce local pressures to offset the effects of increasing global pressures is now widely recognized. This study investigates the spatial and temporal dynamics of coral cover, identifies the main drivers of coral mortality, and quantifies the rates of potential recovery of the Great Barrier Reef. Based on the world’s most extensive time s ...
DOI:
10.1073/pnas.1208909109
PubMed:
23027961
PubMed Central:
PMC3497744

40. A 29-mer site regulates transcription of the initiator gene as well as function of the replication origin of Vibrio cholerae chromosome II

Author:
Tatiana Venkova-Canova; Anik Saha; Dhruba K. Chattoraj
Source:
Plasmid 2012 v.67 no.2 pp. 102-110
ISSN:
0147-619X
Subject:
Escherichia coli; Vibrio cholerae; binding sites; cell cycle; chromosomes; genes; methylation; plasmids; promoter regions; replication origin; transcription (genetics)
Abstract:
... The region responsible for replication of Vibrio cholerae chromosome II (chrII) resembles those of plasmids that have repeated initiator binding sites (iterons) and an autorepressed initiator gene. ChrII has additional features: Its iterons require full methylation for initiator (RctB) binding, which makes them inactive for a part of the cell cycle when they are hemi-methylated. RctB also binds to ...
DOI:
10.1016/j.plasmid.2011.12.009
PubMed:
22248922
PubMed Central:
PMC3319240

41. The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis

Author:
Chikara Masuta; Jun-ichi Inaba; Hanako Shimura
Source:
Plant signaling & behavior 2012 v.7 no.1 pp. 43-45
ISSN:
1559-2324
Subject:
Arabidopsis; Cucumber mosaic virus; RNA interference; catalase; enzyme activity; hydrogen peroxide; necrosis; proteins; sequence homology; virulent strains
Abstract:
... Plant viral symptoms are rarely explained by direct molecular interaction between a viral protein and a host factor, but rather understood as a consequence of arms race between host RNA silencing and viral silencing suppressors. However, we have recently demonstrated that the 2b protein (2b) of Cucumber mosaic virus (CMV) HL strain could bind to Arabidopsis catalase that is important in scavenging ...
DOI:
10.4161/psb.7.1.18526
PubMed:
22301966
PubMed Central:
PMC3357366

42. 3'-UTR-located inverted Alu repeats facilitate mRNA translational repression and stress granule accumulation

Author:
Terry Fitzpatrick; Sui Huang
Source:
Nucleus 2012 v.3 no.4 pp. 359-369
ISSN:
1949-1042
Subject:
gene expression; humans; mutational analysis; nucleotide sequences; reporter genes
Abstract:
... Alu repeats within human genes may potentially alter gene expression. Here, we show that 3′-UTR-located inverted Alu repeats significantly reduce expression of an AcGFP reporter gene. Mutational analysis demonstrates that the secondary structure, but not the primary nucleotide sequence, of the inverted Alu repeats is critical for repression. The expression levels and nucleocytoplasmic distribution ...
DOI:
10.4161/nucl.20827
PubMed:
22688648
PubMed Central:
PMC3679280

43. 3-D and Quasi-2-D Discrete Element Modeling of Grain Commingling in a Bucket Elevator Boot System

Author:
Boac, J.M.; Casada, M.E.; Maghirang, R.G.; Harner, J.P. III
Source:
Transactions of the ASABE 2012 v.55 no.2 pp. 659-672
ISSN:
2151-0032
Subject:
buckets; elevators; grain elevators; grains; mixing; prediction; purity; simulation models; soybeans
Abstract:
... Unwanted grain commingling impedes new quality-based grain handling systems and has proven to be an expensive and time-consuming issue to study experimentally. Experimentally validated models may reduce the time and expense of studying grain commingling while providing additional insight into details of the particle flow. In this study, grain commingling in a pilot-scale bucket elevator boot was f ...
Handle:
10113/54153

44. 3-Dimensional Imaging Modalities for Phenotyping Genetically Engineered Mice

Author:
K. A. Powell; D. Wilson
Source:
Veterinary pathology v.49 no.1 pp. 106-115
ISSN:
1544-2217
Subject:
X-radiation; digital images; embryogenesis; fluorescence; image analysis; mice; microscopy; tomography; ultrasonics
Abstract:
... A variety of 3-dimensional (3D) digital imaging modalities are available for whole-body assessment of genetically engineered mice: magnetic resonance microscopy (MRM), X-ray microcomputed tomography (microCT), optical projection tomography (OPT), episcopic and cryoimaging, and ultrasound biomicroscopy (UBM). Embryo and adult mouse phenotyping can be accomplished at microscopy or near microscopy sp ...
DOI:
10.1177/0300985811429814
PubMed:
22146851
PubMed Central:
PMC3857693

45. 3-Helix Micelles Stabilized by Polymer Springs

Author:
Dong He; Shu Jessica Y.; Dube Nikhil; Ma Yufei; Tirrell Matthew V.; Downing Kenneth H.; Xu Ting
Source:
Journal of the American Chemical Society 2012 v.134 no.28 pp. 11807-11814
ISSN:
1520-5126
Subject:
chemical bonding; colloids; deformation; desorption; energy; hydrophilicity; micelles; nanocarriers; particle size; polyethylene glycol
Abstract:
... Despite increasing demands to employ amphiphilic micelles as nanocarriers and nanoreactors, it remains a significant challenge to simultaneously reduce the particle size and enhance the particle stability. Complementary to covalent chemical bonding and attractive intermolecular interactions, entropic repulsion can be incorporated by rational design in the headgroup of an amphiphile to generate sma ...
DOI:
10.1021/ja3048128
PubMed:
22731391
PubMed Central:
PMC4186246

46. 3-Picolyl Azide Adenine Dinucleotide as a Probe of Femtosecond to Picosecond Enzyme Dynamics

Author:
Dutta Samrat; Li Yun-Liang; Rock William; Houtman Jon C. D.; Kohen Amnon; Cheatum Christopher M.
Source:
Journal of physical chemistry 2012 v.116 no.1 pp. 542-548
ISSN:
1520-5207
Subject:
absorption; active sites; adenine; aqueous solutions; formate dehydrogenase; hydrogen bonding; infrared spectroscopy
Abstract:
... Functionally relevant femtosecond to picosecond dynamics in enzyme active sites can be difficult to measure because of a lack of spectroscopic probes that can be located in the active site without altering the behavior of the enzyme. We have developed a new NAD⁺ analog 3-Picolyl Azide Adenine Dinucleotide (PAAD⁺), which has the potential to be a general spectroscopic probe for NAD-dependent enzyme ...
DOI:
10.1021/jp208677u
PubMed:
22126535
PubMed Central:
PMC3260785

47. IL- 33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G

Author:
Emma Lefrançais; Stephane Roga; Violette Gautier; Anne Gonzalez-de-Peredo; Bernard Monsarrat; Jean-Philippe Girard; Corinne Cayrol
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.5 pp. 1673-1678
ISSN:
0027-8424
Subject:
asthma; basophils; bioactive properties; cardiovascular diseases; caspase-1; cathepsin G; colitis; elastase; eosinophils; humans; inflammation; interleukin-1; interleukin-18; lymphocytes; mast cells; mice; neutrophils; rheumatoid arthritis
Abstract:
... Interleukin-33 (IL-33) (NF-HEV) is a chromatin-associated nuclear cytokine from the IL-1 family, which has been linked to important diseases, including asthma, rheumatoid arthritis, ulcerative colitis, and cardiovascular diseases. IL-33 signals through the ST2 receptor and drives cytokine production in type 2 innate lymphoid cells (ILCs) (natural helper cells, nuocytes), T-helper (Th)2 lymphocytes ...
DOI:
10.1073/pnas.1115884109
PubMed:
22307629
PubMed Central:
PMC3277172

48. IL- 38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist

Author:
Frank L. van de Veerdonk; Angela K. Stoeckman; Gouping Wu; Aaron N. Boeckermann; Tania Azam; Mihai G. Netea; Leo A. B. Joosten; Jos W. M. van der Meer; Ruyi Hao; Vassili Kalabokis; Charles A. Dinarello
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.8 pp. 3001-3005
ISSN:
0027-8424
Subject:
Candida albicans; amino acids; antagonists; dendritic cells; humans; interleukin-1; interleukin-6; interleukin-8; mononuclear leukocytes
Abstract:
... The functional role of IL-1 family member 10, recently renamed IL-38, remains unknown. In the present study we aimed to elucidate the biological function of IL-38 and to identify its receptor. Heat-killed Candida albicans was used to stimulate memory T-lymphocyte cytokine production in freshly obtained human peripheral blood mononuclear cells from healthy subjects. The addition of recombinant IL-3 ...
DOI:
10.1073/pnas.1121534109
PubMed:
22315422
PubMed Central:
PMC3286950

49. 3D cell entrapment in crosslinked thiolated gelatin-poly(ethylene glycol) diacrylate hydrogels

Author:
Yao Fu; Kedi Xu; Xiaoxiang Zheng; Alan J. Giacomin; Adam W. Mix; Weiyuan J. Kao
Source:
Biomaterials 2012 v.33 no.1 pp. 48-58
ISSN:
0142-9612
Subject:
crosslinking; cysteine; ethylene glycol; fibroblasts; gelatin; hydrocolloids; mechanical properties; physicochemical properties; thiols; tissue engineering; water content
Abstract:
... The combined use of natural ECM components and synthetic materials offers an attractive alternative to fabricate hydrogel-based tissue engineering scaffolds to study cell-matrix interactions in three-dimensions (3D). A facile method was developed to modify gelatin with cysteine via a bifunctional PEG linker, thus introducing free thiol groups to gelatin chains. A covalently crosslinked gelatin hyd ...
DOI:
10.1016/j.biomaterials.2011.09.031
PubMed:
21955690
PubMed Central:
PMC3282186

50. 3D imaging and mechanical modeling of helical buckling in Medicago truncatula plant roots

Author:
Jesse L. Silverberg; Roslyn D. Noar; Michael S. Packer; Maria J. Harrison; Christopher L. Henley; Itai Cohen; Sharon J. Gerbode
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.42 pp. 16794-16799
ISSN:
0027-8424
Subject:
Medicago truncatula; culture media; deformation; gels; hydrocolloids; image analysis; mathematical models; root growth; roots
Abstract:
... We study the primary root growth of wild-type Medicago truncatula plants in heterogeneous environments using 3D time-lapse imaging. The growth medium is a transparent hydrogel consisting of a stiff lower layer and a compliant upper layer. We find that the roots deform into a helical shape just above the gel layer interface before penetrating into the lower layer. This geometry is interpreted as a ...
DOI:
10.1073/pnas.1209287109
PubMed:
23010923
PubMed Central:
PMC3479456