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You searched for: Publication year rev 7988-2012 Remove constraint Publication year rev: 7988-2012 Subject liquid chromatography Remove constraint Subject: liquid chromatography Subject term databases Remove constraint Subject term: databases Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A proteome reference map and virulence factors analysis of Yersinia pestis 91001

Author:
Lei Zhou; Wantao Ying; Yanping Han; Ming Chen; Yanfeng Yan; Lei Li; Ziwen Zhu; Zhaobin Zheng; Wei Jia; Ruifu Yang; Xiaohong Qian
Source:
Journal of proteomics 2012 v.75 no.3 pp. 894-907
ISSN:
1874-3919
Subject:
transcriptomics; proteome; Yersinia pestis; hosts; polyacrylamide gel electrophoresis; virulence; peptides; DNA microarrays; mice; plasminogen activator; type III secretion system; Siphonaptera; genes; proteomics; liquid chromatography; databases
Abstract:
... In this report, we carried out the in-depth proteomic analysis of Yersinia pestis strain 91001 under in vitro flea-simulated condition using three technique routes, SDS-PAGE combined with LTQ-FT, two-dimensional liquid chromatography peptide (2D-LC peptide) separation combined with LTQ-FT and intact protein separation followed by 2D-LC peptide separation combined with LTQ-FT. Totally, 1926 protein ...
DOI:
10.1016/j.jprot.2011.10.004
PubMed:
22040741

2. Analysis of Soluble Lignin in Sugarcane by Ultrahigh Performance Liquid Chromatography–Tandem Mass Spectrometry with a Do-It-Yourself Oligomer Database

Author:
Kiyota Eduardo; Mazzafera Paulo; Sawaya Alexandra C. H. F.
Source:
Analytical chemistry 2012 v.84 no.16 pp. 7015-7020
ISSN:
1520-6882
Subject:
biomass; cell walls; databases; enzymes; ethanol production; lignin; lignocellulose; liquid chromatography; sugarcane; tandem mass spectrometry
Abstract:
... Lignin is a polymer found in the cell wall of plants and is one of the main obstacles to the implementation of second-generation ethanol production because it confers the recalcitrance of the lignocellulosic material. The recalcitrance of biomass is affected by the amount of lignin, by its monomer composition, and the way the monomers are arranged in the plant cell wall. Analysis of lignin structu ...
DOI:
10.1021/ac301112y

3. Analysis of Veterinary Drug Residues in Frog Legs and Other Aquacultured Species Using Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry

Author:
Turnipseed Sherri B.; Clark Susan B.; Storey Joseph M.; Carr Justin R.
Source:
Journal of agricultural and food chemistry 2012 v.60 no.18 pp. 4430-4439
ISSN:
1520-5118
Subject:
acetic acid; centrifugation; chloramphenicol; databases; drug residues; fish; fluoroquinolones; frogs; ions; legs; liquid chromatography; mass spectrometry; metabolites; molecular weight; screening; sodium chloride; sulfamethoxazole; trimethoprim; veterinary drugs
Abstract:
... A liquid chromatography quadrupole time-of-flight (Q-TOF) mass spectrometry method was developed to analyze veterinary drug residues in frog legs and other aquacultured species. Samples were extracted using a procedure based on a method developed for the analysis of fluoroquinolones (FQs) in fish. Briefly, the tissue was extracted with dilute acetic acid and acetonitrile with added sodium chloride ...
DOI:
10.1021/jf2049905
PubMed:
22390215

4. Characterization of the Mycobacterium tuberculosis Proteome by Liquid Chromatography Mass Spectrometry-based Proteomics Techniques: A Comprehensive Resource for Tuberculosis Research

Author:
Bell Christina; Smith Geoffrey T.; Sweredoski Michael J.; Hess Sonja
Source:
Journal of proteome research 2012 v.11 no.1 pp. 119-130
ISSN:
1535-3907
Subject:
Mycobacterium tuberculosis; agglutinins; bacteria; bacterial antigens; cell walls; culture filtrates; cytosol; data collection; databases; fractionation; host-pathogen relationships; liquid chromatography; mass spectrometry; pathogenicity; peptides; plasma membrane; proteome; proteomics; transmembrane proteins; vaccines; wheat germ
Abstract:
... Approximately, one-third of the world’s population is infected with Mycobacterium tuberculosis, the causative agent of Tuberculosis. Secreted and membrane proteins that interact with the host play important roles for the pathogenicity of the bacteria and are potential drug targets or components of vaccines. In this present study, subcellular fractionation in combination with membrane enrichment wa ...
DOI:
10.1021/pr2007939

5. Comparative Proteomic Analysis of Differentially Expressed Proteins between Peripheral Sensory and Motor Nerves

Author:
He Qianru; Man Lili; Ji Yuhua; Zhang Shuqiang; Jiang Maorong; Ding Fei; Gu Xiaosong
Source:
Journal of Proteome Research 2012 v.11 no.6 pp. 3077-3089
ISSN:
1535-3907
Subject:
Western blotting; catalytic activity; cell adhesion; cytoskeleton; databases; extracellular matrix; immunohistochemistry; lipid metabolism; liquid chromatography; motor neurons; nerve tissue; neuroplasticity; protein composition; protein synthesis; proteins; proteome; proteomics; quantitative polymerase chain reaction; rats; reverse transcriptase polymerase chain reaction; signal transduction; synaptosomes; tandem mass spectrometry
Abstract:
... Peripheral sensory and motor nerves have different functions and different approaches to regeneration, especially their distinct ability to accurately reinervate terminal nerve pathways. To understand the molecular aspects underlying these differences, the proteomics technique by coupling isobaric tags for relative and absolute quantitation (iTRAQ) with online two-dimensional liquid chromatography ...
DOI:
10.1021/pr300186t

6. Comparison of sample preparation approaches for phospholipids profiling in human serum by liquid chromatography–tandem mass spectrometry

Author:
C. Ferreiro-Vera; F. Priego-Capote; M.D. Luque de Castro
Source:
Journal of chromatography 2012 v.1240 no. pp. 21-28
ISSN:
0021-9673
Subject:
blood serum; databases; fractionation; humans; ionization; liquid chromatography; liquid-liquid extraction; metabolites; metabolome; metabolomics; phospholipids; solid phase extraction; tandem mass spectrometry
Abstract:
... Fractionation of biological fluids for proper analysis by LC–MS of low-abundance metabolites in the presence of high-abundant endogenous metabolites is of interest, particularly when the latter cause undesirable phenomena such as ionization suppression, as is the case with phospholipids (PLs). An exhaustive study and comparison of different protocols for sample preparation based on deproteinizatio ...
DOI:
10.1016/j.chroma.2012.03.074

7. Comprehensive Phosphoproteome Analysis of INS-1 Pancreatic Beta-Cells using Various Digestion Strategies Coupled with Liquid Chromatography–Tandem Mass Spectrometry

Author:
Han Dohyun; Moon Sungyoon; Kim Yikwon; Ho Won-Kyung; Kim Kyunggon; Kang Yup; Jun Heesook; Kim Youngsoo
Source:
Journal of Proteome Research 2012 v.11 no.4 pp. 2206-2223
ISSN:
1535-3907
Subject:
bioinformatics; databases; humans; islets of Langerhans; liquid chromatography; mice; mitochondria; noninsulin-dependent diabetes mellitus; phosphoproteins; phosphorylation; proteome; proteomics; rats; stoichiometry; tandem mass spectrometry; titanium dioxide
Abstract:
... Type 2 diabetes results from aberrant regulation of the phosphorylation cascade in beta-cells. Phosphorylation in pancreatic beta-cells has not been examined extensively, except with regard to subcellular phosphoproteomes using mitochondria. Thus, robust, comprehensive analytical strategies are needed to characterize the many phosphorylated proteins that exist, because of their low abundance, the ...
DOI:
10.1021/pr200990b

8. Enantiomer-specific determination of hexabromocyclododecane in fish by supramolecular solvent-based single-step sample treatment and liquid chromatography–tandem mass spectrometry

Author:
A.B. Lara; C. Caballo; M.D. Sicilia; S. Rubio
Source:
Analytica chimica acta 2012 v.752 no. pp. 62-68
ISSN:
0003-2670
Subject:
bass; beta-cyclodextrin; centrifugation; cod (fish); databases; decanoic acid; enantiomers; hake; liquid chromatography; methanol; microextraction; mixing; monitoring; solvents; tandem mass spectrometry; whiting
Abstract:
... A single-step, environmentally friendly sample treatment was developed and used in combination with liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the quantitation of hexabromocyclododecane (HBCD) stereoisomers in fish. It was based on the microextraction of the stereoisomers with a supramolecular solvent (SUPRAS) made up of reverse aggregates of decanoic acid (DeA). The procedure i ...
DOI:
10.1016/j.aca.2012.09.039

9. Enhanced Analyte Detection Using In-Source Fragmentation of Field Asymmetric Waveform Ion Mobility Spectrometry-Selected Ions in Combination with Time-of-Flight Mass Spectrometry

Author:
Brown Lauren J.; Smith Robert W.; Toutoungi Danielle E.; Reynolds James C.; Bristow Anthony W. T.; Ray Andrew; Sage Ashley; Wilson Ian D.; Weston Daniel J.; Boyle Billy; Creaser Colin S.
Source:
Analytical chemistry 2012 v.84 no.9 pp. 4095-4103
ISSN:
1520-6882
Subject:
blood proteins; databases; dissociation; humans; ions; liquid chromatography; mass spectrometry; peptides; quantitative analysis; spectrometers
Abstract:
... Miniaturized ultra high field asymmetric waveform ion mobility spectrometry (FAIMS) is used for the selective transmission of differential mobility-selected ions prior to in-source collision-induced dissociation (CID) and time-of-flight mass spectrometry (TOFMS) analysis. The FAIMS-in-source collision induced dissociation-TOFMS (FISCID-MS) method requires only minor modification of the ion source ...
DOI:
10.1021/ac300212r

10. Extensive Characterization of Tupaia belangeri Neuropeptidome Using an Integrated Mass Spectrometric Approach

Author:
Petruzziello Filomena; Fouillen Laetitia; Wadensten Henrik; Kretz Robert; Andren Per E.; Rainer Gregor; Zhang Xiaozhe
Source:
Journal of Proteome Research 2012 v.11 no.2 pp. 886-896
ISSN:
1535-3907
Subject:
Primates; Tupaia belangeri; brain; databases; endocrine system; genome; humans; liquid chromatography; mass spectrometry; mice; models; neuropeptides; phylogeny; proteome; rats; sequence homology
Abstract:
... Neuropeptidomics is used to characterize endogenous peptides in the brain of tree shrews (Tupaia belangeri). Tree shrews are small animals similar to rodents in size but close relatives of primates, and are excellent models for brain research. Currently, tree shrews have no complete proteome information available on which direct database search can be allowed for neuropeptide identification. To in ...
DOI:
10.1021/pr200709j

11. Fish peripheral blood mononuclear cells preparation for future monitoring applications

Author:
Marie-Aline Pierrard; Kathleen Roland; Patrick Kestemont; Marc Dieu; Martine Raes; Frédéric Silvestre
Source:
Analytical biochemistry 2012 v.426 no.2 pp. 153-165
ISSN:
0003-2697
Subject:
Anguilla anguilla; National Center for Biotechnology Information; Pangasianodon hypophthalmus; catfish; computer software; databases; ecotoxicology; eel; electrophoresis; endangered species; environmental assessment; fish culture; gene expression regulation; liquid chromatography; monitoring; mononuclear leukocytes; protein synthesis; proteins; proteomics; tandem mass spectrometry; toxicity
Abstract:
... Fish species possess many specific characteristics that support their use in ecotoxicology. Widely used in clinical research, peripheral blood mononuclear cells (PBMCs) can reasonably be exploited as relevant target cells in the assessment of environmental chemical toxicity. The current article focuses on the methods necessary to isolate, characterize, and culture fish PBMCs. These procedures were ...
DOI:
10.1016/j.ab.2012.04.009

12. Higher Energy Collision Dissociation (HCD) Product Ion-Triggered Electron Transfer Dissociation (ETD) Mass Spectrometry for the Analysis of N-Linked Glycoproteins

Author:
Singh Charandeep; Zampronio Cleidiane G.; Creese Andrew J.; Cooper Helen J.
Source:
Journal of Proteome Research 2012 v.11 no.9 pp. 4517-4525
ISSN:
1535-3907
Subject:
N-acetylglucosamine; algorithms; data analysis; databases; dissociation; electron transfer; energy; glycopeptides; glycoproteins; glycoproteomics; immunoglobulin G; ions; liquid chromatography; mannose; mass spectrometry; proteome; ribonucleases; spectrometers; trypsin
Abstract:
... Large scale mass spectrometry analysis of N-linked glycopeptides is complicated by the inherent complexity of the glycan structures. Here, we evaluate a mass spectrometry approach for the targeted analysis of N-linked glycopeptides in complex mixtures that does not require prior knowledge of the glycan structures or pre-enrichment of the glycopeptides. Despite the complexity of N-glycans, the core ...
DOI:
10.1021/pr300257c

13. Improvement of Phosphoproteome Analyses Using FAIMS and Decision Tree Fragmentation. Application to the Insulin Signaling Pathway in Drosophila melanogaster S2 Cells

Author:
Bridon Gaëlle; Bonneil Eric; Muratore-Schroeder Tara; Caron-Lizotte Olivier; Thibault Pierre
Source:
Journal of Proteome Research 2012 v.11 no.2 pp. 927-940
ISSN:
1535-3907
Subject:
Drosophila melanogaster; databases; decision support systems; dissociation; electron transfer; insulin; ions; liquid chromatography; mass spectrometry; phosphopeptides; phosphoproteins; proteome; proteomics; signal transduction
Abstract:
... This report examines the analytical benefits of high-field asymmetric waveform ion mobility spectrometry (FAIMS) coupled to liquid chromatography mass spectrometry (LC–MS) for phosphoproteomics analyses. The ability of FAIMS to separate multiply charged peptide ions from chemical interferences confers a unique advantage in phosphoproteomics by enhancing the detection of low abundance phosphopeptid ...
DOI:
10.1021/pr200722s

14. In-Vial Dual Extraction for Direct LC-MS Analysis of Plasma for Comprehensive and Highly Reproducible Metabolic Fingerprinting.

Author:
Whiley Luke; Godzien Joanna; Ruperez Francisco J; Legido-Quigley Cristina; Barbas Coral
Source:
Analytical chemistry 2012 v.84 no.14 pp. 5992-5999
ISSN:
1520-6882
Subject:
animal models; biomarkers; databases; diabetes; drying; evaporation; fatty acids; hydrophilicity; ionization; lipid composition; liquid chromatography; mass spectrometry; metabolites; methanol; rats; sphingolipids; sterols; tissues
Abstract:
... Metabolic fingerprinting of biological tissues has become an important area of research, particularly in the biomarker discovery field. Methods have inherent analytical variation, and new approaches are necessary to ensure that the vast numbers of intact metabolites present in biofluids are detected. Here, we describe an in-vial dual extraction (IVDE) method and a direct injection method that show ...
DOI:
10.1021/ac300716u

15. Integration of Metabolomics and Expression of Glycerol-3-phosphate Acyltransferase (GPAM) in Breast CancerLink to Patient Survival, Hormone Receptor Status, and Metabolic Profiling

Author:
Brockmöller Scarlet F.; Bucher Elmar; Müller Berit M.; Budczies Jan; Hilvo Mika; Griffin Julian L.; Orešič Matej; Kallioniemi Olli; Iljin Kristiina; Loibl Sibylle; Darb-Esfahani Silvia; Sinn Bruno V.; Klauschen Frederick; Prinzler Judith; Bangemann Nikola; Ismaeel Fakher; Fiehn Oliver; Dietel Manfred; Denkert Carsten
Source:
Journal of Proteome Research 2012 v.11 no.2 pp. 850-860
ISSN:
1535-3907
Subject:
biosynthesis; breast neoplasms; breasts; cell membranes; databases; gas chromatography-mass spectrometry; gene expression; glycerol-3-phosphate acyltransferase; hormone receptors; humans; lipid metabolism; liquid chromatography; messenger RNA; metabolomics; patients; phosphatidylcholines; protein synthesis; triacylglycerols
Abstract:
... Changes in lipid metabolism are an important but not well-characterized hallmark of cancer. On the basis of our recent findings of lipidomic changes in breast cancer, we investigated glycerol-3-phosphate acyltransferase (GPAM), a key enzyme in the lipid biosynthesis of triacylglycerols and phospholipids. GPAM protein expression was evaluated and linked to metabolomic and lipidomic profiles in a co ...
DOI:
10.1021/pr200685r

16. Isolation and determination of the primary structure of a lectin protein from the serum of the American alligator (Alligator mississippiensis)

Author:
Lancia N.F. Darville; Mark E. Merchant; Venkata Maccha; Vivekananda Reddy Siddavarapu; Azeem Hasan; Kermit K. Murray
Source:
Comparative biochemistry and physiology 2012 v.161 no.2 pp. 161-169
ISSN:
1096-4959
Subject:
Alligator mississippiensis; alligators; amino acid sequences; binding capacity; blood serum; computer software; cysteine; databases; humans; lectins; liquid chromatography; mannose; mice; peptides; proteinases; tandem mass spectrometry
Abstract:
... Mass spectrometry in conjunction with de novo sequencing was used to determine the amino acid sequence of a 35kDa lectin protein isolated from the serum of the American alligator that exhibits binding to mannose. The protein N-terminal sequence was determined using Edman degradation and enzymatic digestion with different proteases was used to generate peptide fragments for analysis by liquid chrom ...
DOI:
10.1016/j.cbpb.2011.11.001

17. Large-scale Identification of N-Glycosylated Proteins of Mouse Tissues and Construction of a Glycoprotein Database, GlycoProtDB

Author:
Kaji Hiroyuki; Shikanai Toshihide; Sasaki-Sawa Akiko; Wen Hongling; Fujita Mika; Suzuki Yoshinori; Sugahara Daisuke; Sawaki Hiromichi; Yamauchi Yoshio; Shinkawa Takashi; Taoka Masato; Takahashi Nobuhiro; Isobe Toshiaki; Narimatsu Hisashi
Source:
Journal of Proteome Research 2012 v.11 no.9 pp. 4553-4566
ISSN:
1535-3907
Subject:
databases; drug therapy; gene targeting; glycomics; glycopeptides; glycoproteins; glycoproteomics; glycosylation; liquid chromatography; mass spectrometry; mice; polysaccharides; proteome
Abstract:
... Protein glycosylation is a common post-translational modification that plays important roles in terms of protein function. However, analyzing the relationship between glycosylation and protein function remains technically challenging. This problem arises from the fact that the attached glycans possess diverse and heterogeneous structures. We believe that the first step to elucidate glycan function ...
DOI:
10.1021/pr300346c

18. Mining the Gastric Cancer Secretome: Identification of GRN as a Potential Diagnostic Marker for Early Gastric Cancer

Author:
Loei Hendrick; Tan Hwee Tong; Lim Teck Kwang; Lim Kiat Hon; So Jimmy Bok-Yan; Yeoh Khay Guan; Chung Maxey C. M.
Source:
Journal of Proteome Research 2012 v.11 no.3 pp. 1759-1772
ISSN:
1535-3907
Subject:
biomarkers; blood serum; cell lines; databases; enzyme-linked immunosorbent assay; exosomes; gene expression regulation; immunohistochemistry; liquid chromatography; patients; prediction; prognosis; protein secretion; proteins; proteome; signal peptide; stomach neoplasms; tandem mass spectrometry
Abstract:
... Gastric cancer is the second leading cause of cancer deaths worldwide, and currently, there are no clinically relevant biomarkers for gastric cancer diagnosis or prognosis. In this study, we applied a 2D-LC-MS/MS based approach, in combination with iTRAQ labeling, to study the secretomes of the gastric cancer cell lines AGS and MKN7. By performing a comparative analysis between the conditioned med ...
DOI:
10.1021/pr201014h

19. Occurrence, distribution, and seasonal variation of estrogenic compounds and antibiotic residues in Jiulongjiang River, South China

Author:
Xian Zhang; Dandan Zhang; Han Zhang; Zhuanxi Luo; Changzhou Yan
Source:
Environmental science and pollution research international 2012 v.19 no.5 pp. 1392-1404
ISSN:
0944-1344
Subject:
bisphenol A; databases; diethylstilbestrol; environmental management; estriol; estrone; gas chromatography-mass spectrometry; human health; liquid chromatography; livestock; nonylphenols; octylphenols; ofloxacin; pollution; research; river water; rivers; seasonal variation; sewage; solid phase extraction; sulfadiazine; sulfamerazine; sulfamethazine; sulfamethoxazole; surface water; tandem mass spectrometry; China
Abstract:
... BACKGROUND AND PURPOSE: Estrogenic compounds and antibiotic residues in environment are receiving significant attention because of their potential adverse effects on ecosystems and human health. The objectives of this study were to determine the occurrence and seasonal variability of eight kinds of estrogenic compounds and 14 antibiotics. The study developed an occurrence database of the estrogeni ...
DOI:
10.1007/s11356-012-0818-z

20. Prediction of liquid chromatographic retention for differentiation of structural isomers

Author:
Elli Tyrkkö; Anna Pelander; Ilkka Ojanperä
Source:
Analytica chimica acta 2012 v.720 no. pp. 142-148
ISSN:
0003-2670
Subject:
computer software; databases; diastereomers; drugs; liquid chromatography; mass spectrometry; prediction; reference standards; screening; urine
Abstract:
... A liquid chromatography (LC) retention time prediction software, ACD/ChromGenius, was employed to calculate retention times for structural isomers, which cannot be differentiated by accurate mass measurement techniques alone. For 486 drug compounds included in an in-house database for urine drug screening by liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/Q-TOFMS), a retentio ...
DOI:
10.1016/j.aca.2012.01.024

21. Proteomic Identification of Common SCF Ubiquitin Ligase FBXO6-Interacting Glycoproteins in Three kinds of Cells

Author:
Liu Bin; Zheng Ying; Wang Tong-Dan; Xu Han-Zhang; Xia Li; Zhang Jian; Wu Ying-Li; Chen Guo-Qiang; Wang Li-Shun
Source:
Journal of Proteome Research 2012 v.11 no.3 pp. 1773-1781
ISSN:
1535-3907
Subject:
F-box proteins; cell lines; databases; glycoproteins; liquid chromatography; mass spectrometry; mutants; proteomics; ubiquitin-protein ligase; ubiquitination
Abstract:
... FBOX6 ubiquitin ligase complex is involved in the endoplasmic reticulum-associated degradation pathway by mediating the ubiquitination of glycoproteins. FBXO6 interacts with the chitobiose in unfolded N-glycoprotein, pointing glycoproteins toward E2 for ubiquitination. Although the glycoprotein-recognizing mechanism of FBXO6 is well documented, its bona fide interacting glycoproteins are largely u ...
DOI:
10.1021/pr2010204

22. Proteomic analysis of microvesicles in human saliva by gel electrophoresis with liquid chromatography-mass spectrometry

Author:
Hua Xiao; David T.W. Wong
Source:
Analytica chimica acta 2012 v.723 no. pp. 61-67
ISSN:
0003-2670
Subject:
actin; angiogenesis; biomarkers; cell communication; centrifugation; databases; digestion; exosomes; filtration; humans; immunoglobulins; keratin; liquid chromatography; mass spectrometry; metastasis; peptides; polyacrylamide gel electrophoresis; proteomics; saliva; sodium dodecyl sulfate; trypsin
Abstract:
... Microvesicles (MVs) have been shown to affect the physiology of neighboring recipient cells in various ways. They play an important role in tumor progression/metastasis and angiogenesis in cancer and may be useful therapeutic tools, as well as a mechanism of cell-to-cell communication. They have been visioned as an important biomarker or biomarker source for the detection of different diseases. Hu ...
DOI:
10.1016/j.aca.2012.02.018

23. Proteomic analysis of the pinworm Syphacia muris (Nematoda: Oxyuridae), a parasite of laboratory rats

Author:
Javier Sotillo; María Trelis; Alba Cortés; María Luz Valero; Manuel Sánchez del Pino; José Guillermo Esteban; Antonio Marcilla; Rafael Toledo
Source:
Parasitology international 2012 v.61 no.4 pp. 561-564
ISSN:
1383-5769
Subject:
Syphacia muris; adults; bioassays; biological development; calcium-binding proteins; cell cycle; computer software; databases; enzymes; host-parasite relationships; liquid chromatography; parasites; parasitology; protein metabolism; proteome; proteomics; rats; receptors; tandem mass spectrometry; transmembrane proteins
Abstract:
... Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix- ...
DOI:
10.1016/j.parint.2012.05.004

24. Quantitative Statistical Analysis of Standard and Human Blood Proteins from Liquid Chromatography, Electrospray Ionization, and Tandem Mass Spectrometry

Author:
Bowden Peter; Thavarajah Thanusi; Zhu Peihong; McDonell Mike; Thiele Herbert; Marshall John G.
Source:
Journal of Proteome Research 2012 v.11 no.4 pp. 2032-2047
ISSN:
1535-3907
Subject:
algorithms; analysis of variance; blood; blood proteins; databases; false positive results; humans; ionization; linear models; liquid chromatography; lognormal distribution; peptides; probability; proteome; tandem mass spectrometry
Abstract:
... It will be important to determine if the parent and fragment ion intensity results of liquid chromatography, electrospray ionization and tandem mass spectrometry (LC–ESI–MS/MS) experiments have been randomly and independently sampled from a normal population for the purpose of statistical analysis by general linear models and ANOVA. The tryptic parent peptide and fragment ion m/z and intensity dat ...
DOI:
10.1021/pr2000013

25. Retrospective screening of relevant pesticide metabolites in food using liquid chromatography high resolution mass spectrometry and accurate-mass databases of parent molecules and diagnostic fragment ions

Author:
László Polgár; Juan F. García-Reyes; Péter Fodor; Attila Gyepes; Mihály Dernovics; László Abrankó; Bienvenida Gilbert-López; Antonio Molina-Díaz
Source:
Journal of chromatography 2012 v.1249 no. pp. 83-91
ISSN:
0021-9673
Subject:
computer software; databases; food contamination; fruits; glycosides; glycosylation; ions; liquid chromatography; mass spectrometry; metabolites; pesticide residues; pesticides; screening; vegetables
Abstract:
... In recent years, the detection and characterization of relevant pesticide metabolites in food is an important task in order to evaluate their formation, kinetics, stability, and toxicity. In this article, a methodology for the systematic screening of pesticides and their main metabolites in fruit and vegetable samples is described, using LC–HRMS and accurate-mass database search of parent compound ...
DOI:
10.1016/j.chroma.2012.05.097

26. Screening and Selective Quantification of Illicit Drugs in Wastewater by Mixed-Mode Solid-Phase Extraction and Quadrupole-Time-of-Flight Liquid Chromatography–Mass Spectrometry

Author:
González-Mariño Iria; Quintana José Benito; Rodríguez Isaac; González-Díez Marta; Cela Rafael
Source:
Analytical chemistry 2012 v.84 no.3 pp. 1708-1717
ISSN:
1520-6882
Subject:
adsorbents; cocaine; databases; fractionation; hydrophilicity; illicit drugs; ionization; libraries; liquid chromatography; metabolites; polymers; quantitative analysis; screening; sewage; sewage treatment; solid phase extraction; tandem mass spectrometry; wastewater
Abstract:
... For the first time, a mixed-mode solid-phase extraction with fractionation of basic analytes from neutral and acidic species during cartridge elution and liquid chromatography–quadrupole-time-of-flight mass spectrometry (LC–QTOF-MS) was combined for the quantitative determination of 24 illicit drugs and metabolites in urban sewage samples. The effects of several sample preparation and instrumental ...
DOI:
10.1021/ac202989e

27. Strain-Level Typing and Identification of Bacteria Using Mass Spectrometry-Based Proteomics

Author:
Karlsson Roger; Davidson Max; Svensson-Stadler Liselott; Karlsson Anders; Olesen Kenneth; Carlsohn Elisabet; Moore Edward R. B.
Source:
Journal of Proteome Research 2012 v.11 no.5 pp. 2710-2720
ISSN:
1535-3907
Subject:
Helicobacter pylori; bacteria; databases; liquid chromatography; mass spectrometry; peptides; proteome; proteomics
Abstract:
... Because of the alarming expansion in the diversity and occurrence of bacteria displaying virulence and resistance to antimicrobial agents, it is increasingly important to be able to detect these microorganisms and to differentiate and identify closely related species, as well as different strains of a given species. In this study, a mass spectrometry proteomics approach is applied, exploiting lipi ...
DOI:
10.1021/pr2010633

28. Validation of an LC–MS/MS method for the quantification of choline-related compounds and phospholipids in foods and tissues

Author:
Yeping Xiong; Yuan-Yuan Zhao; Sue Goruk; Kirsten Oilund; Catherine J. Field; René L. Jacobs; Jonathan M. Curtis
Source:
Journal of chromatography 2012 v.911 no. pp. 170-179
ISSN:
1570-0232
Subject:
USDA; animal tissues; chloroform; choline; databases; diet; egg yolk; foods; humans; hydrophilicity; liquid chromatography; methanol; monitoring; nuclear magnetic resonance spectroscopy; phospholipids; tandem mass spectrometry
Abstract:
... A hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC LC–MS/MS) method was developed and validated to simultaneously quantify six aqueous choline-related compounds and eight major phospholipids classes in a single run. HILIC chromatography was coupled to positive ion electrospray mass spectrometry. A combination of multiple scan modes including precursor ion scan, neutral ...
DOI:
10.1016/j.jchromb.2012.10.038