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1. Real-time PCR method for the detection of figwort mosaic virus (FMV) to complement the FMV 34S promoter-specific PCR assay used for screening of genetically modified plants

Author:
Moor, Dominik,   et al.   ; Liniger, Marianne; Grohmann, Lutz; Felleisen, Richard;     Show all 4 Authors
Source:
European food research & technology 2012 v.235 no.5 pp. 835-842
ISSN:
1438-2377
Subject:
DNA; Figwort mosaic virus; cross reaction; detection limit; equipment; nontarget organisms; open reading frames; plant viruses; polymerase chain reaction; screening; transgenic plants
Abstract:
... In this study a new real-time PCR assay for the detection of figwort mosaic virus (FMV) DNA is described. This assay targets a 113-bp-long sequence of the FMV open reading frame Vll, a non-conserved coding region among the caulimoviruses. Detection of FMV DNA is useful to complement screening for the FMV 34S promoter (P-FMV), a genetic element present in several genetically modified (GM) plants. T ...
DOI:
10.1007/s00217-012-1811-y