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- Fan, Guangsen, et al. Show all 6 Authors
- Biotechnology letters 2012 v.34 no.11 pp. 2043-2048
- xylooligosaccharides; Komagataella pastoris; fermenters; xylan; xylanases; Paecilomyces; pH; genes; thermophilic fungi
- ... A xylanase gene from Paecilomyces thermophila was functionally expressed in Pichia pastoris. The recombinant xylanase (xynA) was predominantly extracellular; in a 5 l fermentor culture, the total extracellular protein was 8.1 g l−1 with an activity of 52,940 U ml−1. The enzyme was purified to homogeneity with a recovery of 48 %. The recombinant xynA was optimally active at 75 °C, as measured over ...