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You searched for: Publication year rev 7988-2012 Remove constraint Publication year rev: 7988-2012 Subject term biotechnology Remove constraint Subject term: biotechnology Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. 1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum

Author:
Anniina Kivistö; Ville Santala; Matti Karp
Source:
Journal of biotechnology 2012 v.158 no.4 pp. 242-247
ISSN:
0168-1656
Subject:
Halanaerobium saccharolyticum; absorbance; acetates; bacteria; biotechnology; glycerol; growth retardation; hydrogen production; industry; polyesters; polyurethanes; temperature; vitamin B12
Abstract:
... 1,3-Propanediol (1,3-PD) is widely used in polymer industry in production of polyethers, polyesters and polyurethanes. In this article, a study on 1,3-PD production and tolerance of Halanaerobium saccharolyticum subsp. saccharolyticum is presented. 1,3-PD production was optimized for temperature, vitamin B₁₂ and acetate concentration. The highest 1,3-PD concentrations and yields (0.6mol/mol gl ...
DOI:
10.1016/j.jbiotec.2011.10.013

2. 16. Transplantation of cryopreserved testicular germ cells in flatfish species: a useful technique for reproductive biotechnology

Author:
T. Pacchiarini; C. Sarasquete; E. Olague; M.P. Herráez; E. Cabrita
Source:
Cryobiology 2012 v.65 no.3 pp. 343-344
ISSN:
0011-2240
Subject:
DNA damage; Scophthalmus maximus; Solea senegalensis; aquaculture; biotechnology; breeding stock; busulfan; captive animals; cryopreservation; farmers; females; fish production; genes; in situ hybridization; larvae; larval development; proteins; quantitative polymerase chain reaction; researchers; spermatogonia; survival rate; testes; turbot; viability; xenotransplantation
Abstract:
... Cryopreservation of testicular germ cells plays an important role for biotechnology in fish reproduction. Cryopreserved spermatogonias can be transplanted between closely related species without losing the ability to differentiate in gametes into the host gonads. Due to this capacity, spermatogonia xenotransplantation is becoming a useful tool to produce surrogated broodstocks. These biotechnologi ...
DOI:
10.1016/j.cryobiol.2012.07.015

3. 3,4-Dihydroxycinnamic Acid Attenuates the Fatigue and Improves Exercise Tolerance in Rats

Author:
Rômulo D. NOVAES; Reggiani V. GONÇALVES; Maria do Carmo G. PELUZIO; Antônio J. NATALI; Izabel R. S. C. MALDONADO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.5 pp. 1025-1027
ISSN:
1347-6947
Subject:
3,4-DA; antioxidant activity; biotechnology; blood; blood glucose; exercise; lactic acid; oxidation
Abstract:
... 3,4-Dihydroxycinnamic acid (3,4-DA) is a natural compound with high antioxidant potential found in various foods. This study found that animals administered with 3,4-DA had higher exercise tolerance, reduced blood lactate, and markers of hepatic oxidation. Blood glucose and antioxidant enzymes were not affected by this treatment. 3,4-DA may have applicability in reducing the fatigue associated wit ...
DOI:
10.1271/bbb.111006

4. 6'-Hydroxyoxosorbicillinol, a New Lipoxygenase Inhibitor and PGD₂/LTB₄ Release Suppressor from Penicillium sp.

Author:
Toshikazu KOMODA; Masazumi NISHIKAWA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.7 pp. 1404-1406
ISSN:
1347-6947
Subject:
Penicillium; biotechnology; lipoxygenases; prostaglandins; soybeans
Abstract:
... A new lipoxygenase inhibitor, 6'-hydroxyoxosorbicillinol (1, C₁₄H₁₆O₆), was identified from a culture of Penicillium sp. A known compound, oxosorbicillinol (2, C₁₄H₁₆O₅), was also isolated. Compound 1 showed an approximately 10 times greater inhibitory effect on soybean lipoxygenase (IC₅₀, 16 µM) than 2 (IC₅₀, 150 µM), and also showed prostaglandin D₂ (PGD₂) and leucotriene B₄ (LTB₄) release suppr ...
DOI:
10.1271/bbb.120115

5. A 24-microwell plate with improved mixing and scalable performance for high throughput cell cultures

Author:
Yuan Wen; Ru Zang; Xudong Zhang; Shang-Tian Yang
Source:
Process biochemistry 2012 v.47 no.4 pp. 612-618
ISSN:
1359-5113
Subject:
aeration; agitation; antibody formation; biotechnology; cell culture; cell growth; cell proliferation; cultured cells; drugs; evaporation; glucose; industry; lids; mixers; mixing; oxygen; screening; specific growth rate; viability
Abstract:
... Multi-well plates are widely used in high throughput drug screening, cell clone development, media design and cell culture optimization in the biotechnology industry. The reproducibility and data quality of cell cultures in multi-well plates are greatly affected by mixing, aeration, and evaporation. A novel 24-microwell plate (MWP) with static mixers for improved mixing and aeration, and gas perme ...
DOI:
10.1016/j.procbio.2011.12.023

6. A Caenorhabditis elegans Insulin-Like Peptide, INS-17: Its Physiological Function and Expression Pattern

Author:
Yohei MATSUNAGA; Kensuke NAKAJIMA; Keiko GENGYO-ANDO; Shohei MITANI; Takashi IWASAKI; Tsuyoshi KAWANO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.11 pp. 2168-2172
ISSN:
1347-6947
Subject:
Caenorhabditis elegans; adults; antagonists; biotechnology; diapause; insulin; larvae; longevity; reporter genes
Abstract:
... The insulin/insulin-like growth factor-1 signaling pathway of Caenorhabditis elegans regulates larval diapause and adult lifespan through the sole insulin receptor-like protein, DAF-2. In the present study, the physiological function and expression pattern of INS-17, one of the C. elegans insulin-like peptides, were examined by disruption and overexpression of the gene, and by the use of a reporte ...
DOI:
10.1271/bbb.120540

7. A Coauthorship Network as an Indicator for Scientifi c Collaboration: A Case Study for the School of Biology and Biotechnology, National University of Mongolia

Author:
Bazartseren Boldgiv
Source:
Mongolian journal of biological sciences 2012 v.10 no.1-2 pp. 73-79
ISSN:
2225-4994
Subject:
biotechnology; case studies; Mongolia
Abstract:
... This case study analyzes coauthorship collaboration, or lack thereof, among individual faculty members and departments in the School of Biology and Biotechnology of the National University of Mongolia. I found that publication rates and coauthorship networks in impact-factor journals between 2008 and 2012 (as of October 31, 2012) are highly variable among the eight biology departments we studied, ...
DOI:
10.22353/mjbs.2012.10.09

8. A Combination of Flow Cytometry and Traditional Screening Using Chemicals to Isolate High Glutathione-Producing Yeast Mutants

Author:
Hiroaki NISHIUCHI; Yukiko TABIRA; Kazuo YAMAGISHI
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.6 pp. 1085-1090
ISSN:
1347-6947
Subject:
Saccharomyces cerevisiae; biotechnology; cerulenin; flow cytometry; fluorescence; food production; glutathione; yeasts
Abstract:
... Traditional screening using chemicals or flow cytometry (FCM) alone is not sufficient to isolate the high glutathione (GSH)-producing yeast strains used in food production. Therefore, to improve screening efficiency, we investigated a combination of both methods. A mutated Saccharomyces cerevisiae strain was labeled with 5-chloromethylfluorescein diacetate and sorted by FCM according to emitted fl ...
DOI:
10.1271/bbb.110883

9. A Comparative Analysis of the Molecular Characteristics of the Arabidopsis CoA Pyrophosphohydrolases AtNUDX11, 15, and 15a

Author:
Daisuke ITO; Kazuya YOSHIMURA; Kazuya ISHIKAWA; Takahisa OGAWA; Takanori MARUTA; Shigeru SHIGEOKA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 139-147
ISSN:
1347-6947
Subject:
Arabidopsis; alternative splicing; biosynthesis; biotechnology; cytosol; genes; hydrolases; hydrolysis; malonyl coenzyme A; mitochondria; moieties; nucleosides; tricarboxylic acid cycle
Abstract:
... Coenzyme A (CoA) is an essential, ubiquitous cofactor in all biological systems, where it acts as the major acyl group carrier in various central metabolic reactions. Although much is known about CoA biosynthesis, it is unclear how the CoA pool is regulated the various cellular compartments. It has been found that the nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11 and ...
DOI:
10.1271/bbb.110636

10. A Derivative of L-Allo Threonine Alleviates 2,4-Dinitrofluorobenzene-Induced Atopic Dermatitis Indications

Author:
Jin-Chul HEO; Hyeong-U SON; Solomon L. KIM; Sang-Han LEE
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.11 pp. 2021-2025
ISSN:
1347-6947
Subject:
T-lymphocytes; atopic dermatitis; biotechnology; chemistry; ears; eosinophils; histamine; inflammation; interleukin-17; neck; threonine
Abstract:
... LX519290, a synthetic derivative from a combinatorial chemistry library, has been screened for anti-atopic activity, but its biological activities have not yet been elucidated. To assess whether LX519290 has the potential to lessen 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in mice, first we sensitized the skin in the dorsal neck of C57BL/6 mice and re-sensitized the ear skin to d ...
DOI:
10.1271/bbb.120258

11. A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA

Author:
Masato ISHIHARA; Etsuko NISHIMOTO; Shoji YAMASHITA; Yoshimitsu KAKUTA; Makoto KIMURA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.12 pp. 2335-2337
ISSN:
1347-6947
Subject:
Pyrococcus horikoshii; RNA; biotechnology; energy transfer; fluorescence; oligoribonucleotides; ribonucleases
Abstract:
... The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active confo ...
DOI:
10.1271/bbb.120546

12. A Double Mutant between Fission Yeast Telomerase and RecQ Helicase Is Sensitive to Thiabendazole, an Anti-Microtubule Drug

Author:
Shinobu UKIMORI; Naoki KAWABATA; Hideki SHIMADA; Ryota IMANO; Katsunori TAKAHASHI; Masashi YUKAWA; Eiko TSUCHIYA; Masaru UENO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.2 pp. 264-269
ISSN:
1347-6947
Subject:
Schizosaccharomyces pombe; biotechnology; gels; mutants; pulsed-field gel electrophoresis; telomerase; telomeres; thiabendazole
Abstract:
... In the fission yeast Schizosaccharomyces pombe, deletion of trt1 ⁺ causes gradual telomere shortening, while deletion of pot1 ⁺ causes rapid telomere loss. The double mutant between pot1 and RecQ helicase rqh1 is synthetically lethal. We found that the trt1 rqh1 double mutant was not synthetically lethal. The chromosome end fragments in both the trt1Δ rqh1Δ and the trt1Δ rqh1-hd (helicase dead) do ...
DOI:
10.1271/bbb.110606

13. A Historical Perspective for the Catalytic Reaction Mechanism of Glycosidase; So As to Bring about Breakthrough in Confusing Situation

Author:
Seiya CHIBA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.2 pp. 215-231
ISSN:
1347-6947
Subject:
Lewis bases; biotechnology; catalytic activity; glycosidases; models; organic chemistry; reaction mechanisms
Abstract:
... Various catalytic reaction models have been proposed as the reaction mechanisms of glycosidases, but a reasonable and unitary model capable of interpreting both “inverting” and “retaining” glycosidase reactions remains to be established. As for the models proposed to date, the nucleophilic displacement mechanism and the oxocarbenium ion intermediate mechanism are widely known, but recently the for ...
DOI:
10.1271/bbb.110713

14. A MALDI-TOF MS method for the simultaneous and quantitative analysis of neutral and sialylated glycans of CHO-expressed glycoproteins

Author:
Samnang Tep; Marina Hincapie; William S. Hancock
Source:
Carbohydrate research 2012 v.347 no.1 pp. 121-129
ISSN:
0008-6215
Subject:
anthranilic acid; biopharmaceutical industry; biopharmaceuticals; biotechnology; chromatography; fluorescence; fluorescent dyes; glycoproteins; glycosylation; matrix-assisted laser desorption-ionization mass spectrometry; polysaccharides; process control; quantitative analysis
Abstract:
... The development of a MALDI-TOF MS method for the quantitative analysis of the glycosylation of CHO-expressed biotherapeutic glycoproteins shall be presented. The method utilizes a well-established chemistry, reductive amination of glycans, to derivatize glycans with either a light analog (¹²C₇ anthranilic acid) or a heavy analog (¹³C₇ anthranilic acid) to allow for the direct comparison of the alt ...
DOI:
10.1016/j.carres.2011.10.005

15. A Membrane-Integrated Fermentation Reactor System: Its Effects in Reducing the Amount of Sub-Raw Materials for D-Lactic Acid Continuous Fermentation by Sporolactobacillus laevolacticus

Author:
Takashi MIMITSUKA; Kyungsu NA; Ken MORITA; Hideki SAWAI; Shinichi MINEGISHI; Masahiro HENMI; Katsushige YAMADA; Sakayu SHIMIZU; Tetsu YONEHARA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 67-72
ISSN:
1347-6947
Subject:
Sporolactobacillus; batch fermentation; biotechnology; continuous fermentation; industrialization; lactic acid
Abstract:
... Continuous fermentation by retaining cells with a membrane-integrated fermentation reactor (MFR) system was found to reduce the amount of supplied sub-raw material. If the amount of sub-raw material can be reduced, continuous fermentation with the MFR system should become a more attractive process for industrialization, due to decreased material costs and loads during the refinement process. Our f ...
DOI:
10.1271/bbb.110499

16. A New Meroterpenoid, Chrodrimanin C, from YO-2 of Talaromyces sp.

Author:
Hideo HAYASHI; Yuki OKA; Kenji KAI; Kohki AKIYAMA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.4 pp. 745-748
ISSN:
1347-6947
Subject:
Talaromyces; biotechnology; insecticidal properties; meroterpenoids; okara; silkworms; soybeans; spectroscopy
Abstract:
... The new meroterpenoid, chrodrimanin C (3), together with chrodrimanins A (2) and B (1) were isolated from okara (the insoluble residue of whole soybean) that had been fermented with strain YO-2 of Talaromyces sp. Their structures were elucidated by spectroscopic methods. The partial structures of 1 essential for exhibiting insecticidal activity were investigated by using a silkworm assay. The abso ...
DOI:
10.1271/bbb.110858

17. A New Prenylflavonoid Isolated from Propolis Collected in the Solomon Islands

Author:
Saori INUI; Yuko SHIMAMURA; Shuichi MASUDA; Kenichi SHIRAFUJI; Reuben T. MOLI; Shigenori KUMAZAWA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.5 pp. 1038-1040
ISSN:
1347-6947
Subject:
2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; biotechnology; mass spectrometry; propolis
Abstract:
... The new prenylflavonoid, solophenol A (1), together with three known compounds, bonannione A (2), sophoraflavanone A (3) and (2S)-5,7-dihydroxy-4'-methoxy-8-prenylflavanone (4), were isolated from propolis collected from Malaita Island in The Solomon Islands. The structure of each compound was determined by spectroscopic methods, including mass spectrometry and 2D NMR. Compound 1 exhibited potent ...
DOI:
10.1271/bbb.120021

18. A Novel Alkaline Esterase from Sporosarcina sp. nov. Strain eSP04 Catalyzing the Hydrolysis of a Wide Variety of Aryl-carboxylic Acid Esters

Author:
Munenori TAKEHARA; Kaori KINOSHITA; Masahiro MIYAMOTO; Hideo HIROHARA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.9 pp. 1721-1727
ISSN:
1347-6947
Subject:
Sporosarcina; amino acid sequences; biotechnology; esterases; ethylene glycol; hydrolysis; molecular weight; pH; phenylacetic acid; phenylpropionates; temperature
Abstract:
... A novel esterase showing activity specific for esters of aryl-carboxylic acids was discovered in Sporosarcina sp. nov., which was identified by the 16S rDNA sequencing method in addition to morphological and physiological analyses. The aryl-carboxylesterase (named EstAC) was purified 780-fold from crude cell extracts by a 5-step procedure. EstAC was characterized as a monomeric protein with a mole ...
DOI:
10.1271/bbb.120332

19. A Novel Function of the Human Chaperonin CCT Epsilon Subunit in Yeast

Author:
Jun-ichi NIKAWA; Minami KIMURA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 199-201
ISSN:
1347-6947
Subject:
actin; auxotrophs; biotechnology; chaperonins; cytosol; endoplasmic reticulum; humans; inositols; phenotype; tubulin; yeasts
Abstract:
... A malfunction in the yeast HAC1 causes the unfolding-protein response in the endoplasmic reticulum, resulting in stress-sensitive and inositol auxotrophic phenotypes. Chaperonin-containing TCP1 (CCT) is necessary for the folding of actin and tubulin in the cytosol. The introduction of the truncated human CCT epsilon subunit into yeast cells of which hac1 was disrupted clearly suppressed not only i ...
DOI:
10.1271/bbb.110638

20. A Novel Reductase from Candida albicans for the Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate

Author:
Mingdong AN; Ping CAI; Ming YAN; Ning HAO; Shanshan WANG; Huan LIU; Yan LI; Lin XU
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.6 pp. 1210-1212
ISSN:
1347-6947
Subject:
Candida albicans; Escherichia coli; acetates; biocatalysis; biotechnology; glucose; oxidoreductases
Abstract:
... A novel NADPH-dependent reductase (CaCR) from Candida albicans was cloned for the first time. It catalyzed asymmetric reduction to produce ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE). It contained an open reading frame of 843 bp encoding 281 amino acids. When co-expressed with a glucose dehydrogenase in Escherichia coli, recombinant CaCR exhibited an activity of 5.7 U/mg with ethyl 4-chloro-3 ...
DOI:
10.1271/bbb.120048