U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Publication year rev 7988-2012 Remove constraint Publication year rev: 7988-2012 Subject term biotechnology Remove constraint Subject term: biotechnology
Start Over

Search

1,079  Search Results

« Previous | 1 - 50 of 1,079 |
Number of results to display per page

Search Results

1. 1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum

Author:
Anniina Kivistö; Ville Santala; Matti Karp
Source:
Journal of biotechnology 2012 v.158 no.4 pp. 242-247
ISSN:
0168-1656
Subject:
Halanaerobium saccharolyticum; absorbance; acetates; bacteria; biotechnology; glycerol; growth retardation; hydrogen production; industry; polyesters; polyurethanes; temperature; vitamin B12
Abstract:
... 1,3-Propanediol (1,3-PD) is widely used in polymer industry in production of polyethers, polyesters and polyurethanes. In this article, a study on 1,3-PD production and tolerance of Halanaerobium saccharolyticum subsp. saccharolyticum is presented. 1,3-PD production was optimized for temperature, vitamin B₁₂ and acetate concentration. The highest 1,3-PD concentrations and yields (0.6mol/mol gl ...
DOI:
10.1016/j.jbiotec.2011.10.013

2. 16. Transplantation of cryopreserved testicular germ cells in flatfish species: a useful technique for reproductive biotechnology

Author:
T. Pacchiarini; C. Sarasquete; E. Olague; M.P. Herráez; E. Cabrita
Source:
Cryobiology 2012 v.65 no.3 pp. 343-344
ISSN:
0011-2240
Subject:
DNA damage; Scophthalmus maximus; Solea senegalensis; aquaculture; biotechnology; breeding stock; busulfan; captive animals; cryopreservation; farmers; females; fish production; genes; in situ hybridization; larvae; larval development; proteins; quantitative polymerase chain reaction; researchers; spermatogonia; survival rate; testes; turbot; viability; xenotransplantation
Abstract:
... Cryopreservation of testicular germ cells plays an important role for biotechnology in fish reproduction. Cryopreserved spermatogonias can be transplanted between closely related species without losing the ability to differentiate in gametes into the host gonads. Due to this capacity, spermatogonia xenotransplantation is becoming a useful tool to produce surrogated broodstocks. These biotechnologi ...
DOI:
10.1016/j.cryobiol.2012.07.015

3. 3,4-Dihydroxycinnamic Acid Attenuates the Fatigue and Improves Exercise Tolerance in Rats

Author:
Rômulo D. NOVAES; Reggiani V. GONÇALVES; Maria do Carmo G. PELUZIO; Antônio J. NATALI; Izabel R. S. C. MALDONADO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.5 pp. 1025-1027
ISSN:
1347-6947
Subject:
3,4-DA; antioxidant activity; biotechnology; blood; blood glucose; exercise; lactic acid; oxidation
Abstract:
... 3,4-Dihydroxycinnamic acid (3,4-DA) is a natural compound with high antioxidant potential found in various foods. This study found that animals administered with 3,4-DA had higher exercise tolerance, reduced blood lactate, and markers of hepatic oxidation. Blood glucose and antioxidant enzymes were not affected by this treatment. 3,4-DA may have applicability in reducing the fatigue associated wit ...
DOI:
10.1271/bbb.111006

4. 6'-Hydroxyoxosorbicillinol, a New Lipoxygenase Inhibitor and PGD₂/LTB₄ Release Suppressor from Penicillium sp.

Author:
Toshikazu KOMODA; Masazumi NISHIKAWA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.7 pp. 1404-1406
ISSN:
1347-6947
Subject:
Penicillium; biotechnology; lipoxygenases; prostaglandins; soybeans
Abstract:
... A new lipoxygenase inhibitor, 6'-hydroxyoxosorbicillinol (1, C₁₄H₁₆O₆), was identified from a culture of Penicillium sp. A known compound, oxosorbicillinol (2, C₁₄H₁₆O₅), was also isolated. Compound 1 showed an approximately 10 times greater inhibitory effect on soybean lipoxygenase (IC₅₀, 16 µM) than 2 (IC₅₀, 150 µM), and also showed prostaglandin D₂ (PGD₂) and leucotriene B₄ (LTB₄) release suppr ...
DOI:
10.1271/bbb.120115

5. A 24-microwell plate with improved mixing and scalable performance for high throughput cell cultures

Author:
Yuan Wen; Ru Zang; Xudong Zhang; Shang-Tian Yang
Source:
Process biochemistry 2012 v.47 no.4 pp. 612-618
ISSN:
1359-5113
Subject:
aeration; agitation; antibody formation; biotechnology; cell culture; cell growth; cell proliferation; cultured cells; drugs; evaporation; glucose; industry; lids; mixers; mixing; oxygen; screening; specific growth rate; viability
Abstract:
... Multi-well plates are widely used in high throughput drug screening, cell clone development, media design and cell culture optimization in the biotechnology industry. The reproducibility and data quality of cell cultures in multi-well plates are greatly affected by mixing, aeration, and evaporation. A novel 24-microwell plate (MWP) with static mixers for improved mixing and aeration, and gas perme ...
DOI:
10.1016/j.procbio.2011.12.023

6. A Caenorhabditis elegans Insulin-Like Peptide, INS-17: Its Physiological Function and Expression Pattern

Author:
Yohei MATSUNAGA; Kensuke NAKAJIMA; Keiko GENGYO-ANDO; Shohei MITANI; Takashi IWASAKI; Tsuyoshi KAWANO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.11 pp. 2168-2172
ISSN:
1347-6947
Subject:
Caenorhabditis elegans; adults; antagonists; biotechnology; diapause; insulin; larvae; longevity; reporter genes
Abstract:
... The insulin/insulin-like growth factor-1 signaling pathway of Caenorhabditis elegans regulates larval diapause and adult lifespan through the sole insulin receptor-like protein, DAF-2. In the present study, the physiological function and expression pattern of INS-17, one of the C. elegans insulin-like peptides, were examined by disruption and overexpression of the gene, and by the use of a reporte ...
DOI:
10.1271/bbb.120540

7. A Coauthorship Network as an Indicator for Scientifi c Collaboration: A Case Study for the School of Biology and Biotechnology, National University of Mongolia

Author:
Bazartseren Boldgiv
Source:
Mongolian journal of biological sciences 2012 v.10 no.1-2 pp. 73-79
ISSN:
2225-4994
Subject:
biotechnology; case studies; Mongolia
Abstract:
... This case study analyzes coauthorship collaboration, or lack thereof, among individual faculty members and departments in the School of Biology and Biotechnology of the National University of Mongolia. I found that publication rates and coauthorship networks in impact-factor journals between 2008 and 2012 (as of October 31, 2012) are highly variable among the eight biology departments we studied, ...
DOI:
10.22353/mjbs.2012.10.09

8. A Combination of Flow Cytometry and Traditional Screening Using Chemicals to Isolate High Glutathione-Producing Yeast Mutants

Author:
Hiroaki NISHIUCHI; Yukiko TABIRA; Kazuo YAMAGISHI
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.6 pp. 1085-1090
ISSN:
1347-6947
Subject:
Saccharomyces cerevisiae; biotechnology; cerulenin; flow cytometry; fluorescence; food production; glutathione; yeasts
Abstract:
... Traditional screening using chemicals or flow cytometry (FCM) alone is not sufficient to isolate the high glutathione (GSH)-producing yeast strains used in food production. Therefore, to improve screening efficiency, we investigated a combination of both methods. A mutated Saccharomyces cerevisiae strain was labeled with 5-chloromethylfluorescein diacetate and sorted by FCM according to emitted fl ...
DOI:
10.1271/bbb.110883

9. A Comparative Analysis of the Molecular Characteristics of the Arabidopsis CoA Pyrophosphohydrolases AtNUDX11, 15, and 15a

Author:
Daisuke ITO; Kazuya YOSHIMURA; Kazuya ISHIKAWA; Takahisa OGAWA; Takanori MARUTA; Shigeru SHIGEOKA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 139-147
ISSN:
1347-6947
Subject:
Arabidopsis; alternative splicing; biosynthesis; biotechnology; cytosol; genes; hydrolases; hydrolysis; malonyl coenzyme A; mitochondria; moieties; nucleosides; tricarboxylic acid cycle
Abstract:
... Coenzyme A (CoA) is an essential, ubiquitous cofactor in all biological systems, where it acts as the major acyl group carrier in various central metabolic reactions. Although much is known about CoA biosynthesis, it is unclear how the CoA pool is regulated the various cellular compartments. It has been found that the nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11 and ...
DOI:
10.1271/bbb.110636

10. A Derivative of L-Allo Threonine Alleviates 2,4-Dinitrofluorobenzene-Induced Atopic Dermatitis Indications

Author:
Jin-Chul HEO; Hyeong-U SON; Solomon L. KIM; Sang-Han LEE
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.11 pp. 2021-2025
ISSN:
1347-6947
Subject:
T-lymphocytes; atopic dermatitis; biotechnology; chemistry; ears; eosinophils; histamine; inflammation; interleukin-17; neck; threonine
Abstract:
... LX519290, a synthetic derivative from a combinatorial chemistry library, has been screened for anti-atopic activity, but its biological activities have not yet been elucidated. To assess whether LX519290 has the potential to lessen 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in mice, first we sensitized the skin in the dorsal neck of C57BL/6 mice and re-sensitized the ear skin to d ...
DOI:
10.1271/bbb.120258

11. A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA

Author:
Masato ISHIHARA; Etsuko NISHIMOTO; Shoji YAMASHITA; Yoshimitsu KAKUTA; Makoto KIMURA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.12 pp. 2335-2337
ISSN:
1347-6947
Subject:
Pyrococcus horikoshii; RNA; biotechnology; energy transfer; fluorescence; oligoribonucleotides; ribonucleases
Abstract:
... The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active confo ...
DOI:
10.1271/bbb.120546

12. A Double Mutant between Fission Yeast Telomerase and RecQ Helicase Is Sensitive to Thiabendazole, an Anti-Microtubule Drug

Author:
Shinobu UKIMORI; Naoki KAWABATA; Hideki SHIMADA; Ryota IMANO; Katsunori TAKAHASHI; Masashi YUKAWA; Eiko TSUCHIYA; Masaru UENO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.2 pp. 264-269
ISSN:
1347-6947
Subject:
Schizosaccharomyces pombe; biotechnology; gels; mutants; pulsed-field gel electrophoresis; telomerase; telomeres; thiabendazole
Abstract:
... In the fission yeast Schizosaccharomyces pombe, deletion of trt1 ⁺ causes gradual telomere shortening, while deletion of pot1 ⁺ causes rapid telomere loss. The double mutant between pot1 and RecQ helicase rqh1 is synthetically lethal. We found that the trt1 rqh1 double mutant was not synthetically lethal. The chromosome end fragments in both the trt1Δ rqh1Δ and the trt1Δ rqh1-hd (helicase dead) do ...
DOI:
10.1271/bbb.110606

13. A Historical Perspective for the Catalytic Reaction Mechanism of Glycosidase; So As to Bring about Breakthrough in Confusing Situation

Author:
Seiya CHIBA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.2 pp. 215-231
ISSN:
1347-6947
Subject:
Lewis bases; biotechnology; catalytic activity; glycosidases; models; organic chemistry; reaction mechanisms
Abstract:
... Various catalytic reaction models have been proposed as the reaction mechanisms of glycosidases, but a reasonable and unitary model capable of interpreting both “inverting” and “retaining” glycosidase reactions remains to be established. As for the models proposed to date, the nucleophilic displacement mechanism and the oxocarbenium ion intermediate mechanism are widely known, but recently the for ...
DOI:
10.1271/bbb.110713

14. A MALDI-TOF MS method for the simultaneous and quantitative analysis of neutral and sialylated glycans of CHO-expressed glycoproteins

Author:
Samnang Tep; Marina Hincapie; William S. Hancock
Source:
Carbohydrate research 2012 v.347 no.1 pp. 121-129
ISSN:
0008-6215
Subject:
anthranilic acid; biopharmaceutical industry; biopharmaceuticals; biotechnology; chromatography; fluorescence; fluorescent dyes; glycoproteins; glycosylation; matrix-assisted laser desorption-ionization mass spectrometry; polysaccharides; process control; quantitative analysis
Abstract:
... The development of a MALDI-TOF MS method for the quantitative analysis of the glycosylation of CHO-expressed biotherapeutic glycoproteins shall be presented. The method utilizes a well-established chemistry, reductive amination of glycans, to derivatize glycans with either a light analog (¹²C₇ anthranilic acid) or a heavy analog (¹³C₇ anthranilic acid) to allow for the direct comparison of the alt ...
DOI:
10.1016/j.carres.2011.10.005

15. A Membrane-Integrated Fermentation Reactor System: Its Effects in Reducing the Amount of Sub-Raw Materials for D-Lactic Acid Continuous Fermentation by Sporolactobacillus laevolacticus

Author:
Takashi MIMITSUKA; Kyungsu NA; Ken MORITA; Hideki SAWAI; Shinichi MINEGISHI; Masahiro HENMI; Katsushige YAMADA; Sakayu SHIMIZU; Tetsu YONEHARA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 67-72
ISSN:
1347-6947
Subject:
Sporolactobacillus; batch fermentation; biotechnology; continuous fermentation; industrialization; lactic acid
Abstract:
... Continuous fermentation by retaining cells with a membrane-integrated fermentation reactor (MFR) system was found to reduce the amount of supplied sub-raw material. If the amount of sub-raw material can be reduced, continuous fermentation with the MFR system should become a more attractive process for industrialization, due to decreased material costs and loads during the refinement process. Our f ...
DOI:
10.1271/bbb.110499

16. A New Meroterpenoid, Chrodrimanin C, from YO-2 of Talaromyces sp.

Author:
Hideo HAYASHI; Yuki OKA; Kenji KAI; Kohki AKIYAMA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.4 pp. 745-748
ISSN:
1347-6947
Subject:
Talaromyces; biotechnology; insecticidal properties; meroterpenoids; okara; silkworms; soybeans; spectroscopy
Abstract:
... The new meroterpenoid, chrodrimanin C (3), together with chrodrimanins A (2) and B (1) were isolated from okara (the insoluble residue of whole soybean) that had been fermented with strain YO-2 of Talaromyces sp. Their structures were elucidated by spectroscopic methods. The partial structures of 1 essential for exhibiting insecticidal activity were investigated by using a silkworm assay. The abso ...
DOI:
10.1271/bbb.110858

17. A New Prenylflavonoid Isolated from Propolis Collected in the Solomon Islands

Author:
Saori INUI; Yuko SHIMAMURA; Shuichi MASUDA; Kenichi SHIRAFUJI; Reuben T. MOLI; Shigenori KUMAZAWA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.5 pp. 1038-1040
ISSN:
1347-6947
Subject:
2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; biotechnology; mass spectrometry; propolis
Abstract:
... The new prenylflavonoid, solophenol A (1), together with three known compounds, bonannione A (2), sophoraflavanone A (3) and (2S)-5,7-dihydroxy-4'-methoxy-8-prenylflavanone (4), were isolated from propolis collected from Malaita Island in The Solomon Islands. The structure of each compound was determined by spectroscopic methods, including mass spectrometry and 2D NMR. Compound 1 exhibited potent ...
DOI:
10.1271/bbb.120021

18. A Novel Alkaline Esterase from Sporosarcina sp. nov. Strain eSP04 Catalyzing the Hydrolysis of a Wide Variety of Aryl-carboxylic Acid Esters

Author:
Munenori TAKEHARA; Kaori KINOSHITA; Masahiro MIYAMOTO; Hideo HIROHARA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.9 pp. 1721-1727
ISSN:
1347-6947
Subject:
Sporosarcina; amino acid sequences; biotechnology; esterases; ethylene glycol; hydrolysis; molecular weight; pH; phenylacetic acid; phenylpropionates; temperature
Abstract:
... A novel esterase showing activity specific for esters of aryl-carboxylic acids was discovered in Sporosarcina sp. nov., which was identified by the 16S rDNA sequencing method in addition to morphological and physiological analyses. The aryl-carboxylesterase (named EstAC) was purified 780-fold from crude cell extracts by a 5-step procedure. EstAC was characterized as a monomeric protein with a mole ...
DOI:
10.1271/bbb.120332

19. A Novel Function of the Human Chaperonin CCT Epsilon Subunit in Yeast

Author:
Jun-ichi NIKAWA; Minami KIMURA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 199-201
ISSN:
1347-6947
Subject:
actin; auxotrophs; biotechnology; chaperonins; cytosol; endoplasmic reticulum; humans; inositols; phenotype; tubulin; yeasts
Abstract:
... A malfunction in the yeast HAC1 causes the unfolding-protein response in the endoplasmic reticulum, resulting in stress-sensitive and inositol auxotrophic phenotypes. Chaperonin-containing TCP1 (CCT) is necessary for the folding of actin and tubulin in the cytosol. The introduction of the truncated human CCT epsilon subunit into yeast cells of which hac1 was disrupted clearly suppressed not only i ...
DOI:
10.1271/bbb.110638

20. A Novel Reductase from Candida albicans for the Production of Ethyl (S)-4-Chloro-3-hydroxybutanoate

Author:
Mingdong AN; Ping CAI; Ming YAN; Ning HAO; Shanshan WANG; Huan LIU; Yan LI; Lin XU
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.6 pp. 1210-1212
ISSN:
1347-6947
Subject:
Candida albicans; Escherichia coli; acetates; biocatalysis; biotechnology; glucose; oxidoreductases
Abstract:
... A novel NADPH-dependent reductase (CaCR) from Candida albicans was cloned for the first time. It catalyzed asymmetric reduction to produce ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE). It contained an open reading frame of 843 bp encoding 281 amino acids. When co-expressed with a glucose dehydrogenase in Escherichia coli, recombinant CaCR exhibited an activity of 5.7 U/mg with ethyl 4-chloro-3 ...
DOI:
10.1271/bbb.120048

21. A PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome

Author:
Anna A. Ublinskaya; Valeriy V. Samsonov; Sergey V. Mashko; Nataliya V. Stoynova
Source:
Journal of microbiological methods 2012 v.89 no.3 pp. 167-173
ISSN:
0167-7012
Subject:
DNA fragmentation; loci; genome; industry; plasmid vectors; polymerase chain reaction; bacteriophages; biotechnology; homologous recombination; genetic engineering; Escherichia coli
Abstract:
... The genetic manipulation of cells is the most promising strategy for designing microorganisms with desired traits. The most widely used approaches for integrating specific DNA-fragments into the Escherichia coli genome are based on bacteriophage site-specific and Red/ET-mediated homologous recombination systems. Specifically, the recently developed Dual In/Out integration strategy enables the inte ...
DOI:
10.1016/j.mimet.2012.03.013

22. A Polyphenol Extract of Hibiscus sabdariffa L. Ameliorates Acetaminophen-Induced Hepatic Steatosis by Attenuating the Mitochondrial Dysfunction in Vivo and in Vitro

Author:
Chao-Hsin LEE; Chih-Yi KUO; Chau-Jong WANG; Chi-Ping WANG; Yi-Ru LEE; Chi-Nan HUNG; Huei-Jane LEE
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.4 pp. 646-651
ISSN:
1347-6947
Subject:
Hibiscus sabdariffa; acetaminophen; antioxidant activity; biotechnology; blood; catalase; cell death; death; fatty liver; glutathione; histopathology; lipid peroxidation; liver; membrane potential; mitochondria; mitochondrial membrane; oxidative stress; polyphenols
Abstract:
... Oxidative stress is the major contributor to acetaminophen (AAP)-caused liver damage. It promotes mitochondrial oxidative stress and collapses the mitochondrial membrane potential to cause cell death. We have previously shown that a polyphenol extract of Hibiscus sabdariffa L. (HPE) potentiated the antioxidative effect. We further examined in this study the possible mechanism of HPE against AAP-ca ...
DOI:
10.1271/bbb.110579

23. A Rapid and Simple Method of Evaluating the Dimeric Tendency of Fluorescent Proteins in Living Cells Using a Truncated Protein of Importin α as Fusion Tag

Author:
Chika NAKAGAWA; Shigenori NISHIMURA; Kaori SENDA-MURATA; Kenji SUGIMOTO
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.2 pp. 388-390
ISSN:
1347-6947
Subject:
biotechnology; fluorescence; green fluorescent protein; hydrophobicity; importins; mammals; mutation
Abstract:
... Enhanced green fluorescent protein (EGFP) and its yellow variant (Venus) are weakly dimeric under physiological conditions. We designed a simple method to evaluate the dimeric tendency of fluorescent proteins in living mammalian cells. A novel single mutation, A206L, interfering with the hydrophobic interactions of the dimer interface in Venus, contributed to its monomerization, and was as effecti ...
DOI:
10.1271/bbb.110677

24. A Review on the Challenges for Increased Production of Castor

Author:
Liv S. Severino; Dick L. Auld; Marco Baldanzi; Magno J. D. Cândido; Grace Chen; William Crosby; D. Tan; Xiaohua He; P. Lakshmamma; C. Lavanya; Olga L. T. Machado; Thomas Mielke; Máira Milani; Travis D. Miller; J. B. Morris; Stephen. A. Morse; Alejandro A. Navas; Dartanhã J. Soares; Valdinei Sofiatti; Ming L. Wang; Maurício D. Zanotto; Helge Zieler
Source:
Agronomy journal 2012 v.104 no.4 pp. 853-880
ISSN:
0002-1962
Subject:
Ricinus communis; abiotic stress; biotechnology; biotic stress; breeding; castor bean meal; castor oil; chemical industry; crops; drought; fatty acid composition; feeds; fuels; genetics; genome; germplasm; languages; lipid content; oilseed crops; organic fertilizers; prices; researchers; ricinoleic acid; scientists; toxins; vegetable oil
Abstract:
... Castor (Ricinus communis L.) is one of the oldest cultivated crops, but currently it represents only 0.15% of the vegetable oil produced in the world. Castor oil is of continuing importance to the global specialty chemical industry because it is the only commercial source of a hydroxylated fatty acid. Castor also has tremendous future potential as an industrial oilseed crop because of its high see ...
DOI:
10.2134/agronj2011.0210

25. A Secreted Placental Alkaline Phosphatase-Based Reporter Assay System for Screening of Compounds Acting at an Octopamine Receptor Stably Expressed in a Mammalian Cell Line

Author:
Hiroto OHTA; Hiroshi OSHIUMI; Naotaka HAYASHI; Tetsuya IMAI; Yoshihisa OZOE; Shigeru MORIMURA; Kenji KIDA
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.1 pp. 209-211
ISSN:
1347-6947
Subject:
alkaline phosphatase; biotechnology; cell lines; chemiluminescence; insecticides; mammals; octopamine; reporter genes; silkworms; transcription (genetics)
Abstract:
... Octopamine receptors are attractive insecticide targets. To screen compounds acting at octopamine receptors simply and rapidly, we constructed a chemiluminescent reporter gene assay system that detects secreted placental alkaline phosphatase transcriptionally regulated by the cAMP response element for a silkworm octopamine receptor. This system proved useful in high-throughput screening to develop ...
DOI:
10.1271/bbb.110690

26. A Single Residue Determines the Cooperative Binding Property of a Primosomal DNA Replication Protein, PriB, to Single-Stranded DNA

Author:
Yen-Hua HUANG; Hsin-Hsien LIN; Cheng-Yang HUANG
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.6 pp. 1110-1115
ISSN:
1347-6947
Subject:
DNA replication; Escherichia coli; Salmonella Typhimurium; biotechnology; nucleotides; single-stranded DNA
Abstract:
... PriB is a primosomal protein required for re-initiation of replication in bacteria. We characterized and compared the DNA-binding properties of PriB from Salmonella enterica serovar Typhimurium LT2 (StPriB) and Escherichia coli (EcPriB). Only one residue of EcPriB, V6, was different in StPriB (replaced by A6). Previous structural information revealed that this residue is located on the putative di ...
DOI:
10.1271/bbb.110938

27. A Spatial Relationship between Sheath Elongation and Cell Proliferation in Sphaerotilus natans

Author:
Minoru TAKEDA; Takuto UMEZU; Yuta KAWASAKI; Shoichi SHIMURA; Keiko KONDO; Jun-ichi KOIZUMI
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.12 pp. 2357-2359
ISSN:
1347-6947
Subject:
Sphaerotilus natans; bacteria; biochemistry; biotechnology; cell growth; cell proliferation; fluorescent labeling; models; phase-contrast microscopy
Abstract:
... Sphaerotilus natans is a filamentous sheath-forming bacterium. A method of selective fluorescent-labeling of its sheath using conventional reagents was developed. Terminal expansion of the sheath was confirmed by this method. In addition, ubiquitous cell growth was revealed by sequential phase-contrast microscopy of a filament. Based on this and earlier reports, a model of the sheath formation is ...
DOI:
10.1271/bbb.120616

28. A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes

Author:
Solmaz Arvani; Annette Markert; Anita Loeschcke; Karl-Erich Jaeger; Thomas Drepper
Source:
Journal of biotechnology 2012 v.159 no.3 pp. 162-171
ISSN:
0168-1656
Subject:
DNA-directed RNA polymerase; RNA; Rhodobacter capsulatus; bacteria; biochemical pathways; biotechnology; ferredoxin hydrogenase; gene overexpression; genes; loci; plasmids; proteins
Abstract:
... Bacterial genes whose enzymes are either assembled into complex multi-domain proteins or form biosynthetic pathways are frequently organized within large chromosomal clusters. The functional expression of clustered genes, however, remains challenging since it generally requires an expression system that facilitates the coordinated transcription of numerous genes irrespective of their natural promo ...
DOI:
10.1016/j.jbiotec.2012.01.004

29. A Tobacco CBL-Interacting Protein Kinase Homolog Is Involved in Phosphorylation of the N-Terminal Domain of the Cucumber Mosaic Virus Polymerase 2a Protein

Author:
Hyun Ku KANG; Seung Hwan YANG; Young Pyo LEE; Young In PARK; Sang Hyon KIM
Source:
Bioscience, biotechnology, and biochemistry 2012 v.76 no.11 pp. 2101-2106
ISSN:
1347-6947
Subject:
Cucumber mosaic virus; RNA-directed RNA polymerase; biotechnology; cytosol; phosphorylation; protein kinases; tobacco; two hybrid system techniques
Abstract:
... The replication and transcription of cucumber mosaic virus (CMV) are catalyzed by multi-protein complex RNA-dependent RNA polymerase (RdRp), which is composed of the viral-encoded 1a and 2a proteins with host factors. We have reported that the N-terminal region of the polymerase 2a protein, composed of 126 amino acids, is required for interaction with the helicase 1a protein, and that the phosphor ...
DOI:
10.1271/bbb.120474

30. A bioremediation case of an ex-quarry area restored by paper sludge

Author:
Alessandra Bonoli; Alice Dall’Ara
Source:
Journal of biotechnology 2012 v.157 no.4 pp. 499-504
ISSN:
0168-1656
Subject:
anaerobic conditions; biogas; bioremediation; biotechnology; monitoring; paper; public health; pulp and paper industry; pulp and paper sludge; recycling; sludge
Abstract:
... Most paper industry waste is in the form of sludge from paper production and recycle process paper. There has been an increasing use of paper sludge in environmental restoration, a practice that requires particular attention. This issue presents a case which demonstrates how the biogas production related to this kind of recovery system can represent a problem for environmental protection and publi ...
DOI:
10.1016/j.jbiotec.2011.08.010

31. A biosensor for cholesterol based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence

Author:
Meihe Zhang; Ruo Yuan; Yaqin Chai; Shihong Chen; Huaan Zhong; Cun Wang; Yinfeng Cheng
Source:
Biosensors & bioelectronics 2012 v.32 no.1 pp. 288-292
ISSN:
0956-5663
Subject:
atomic force microscopy; biosensors; biotechnology; chemiluminescence; cholesterol; detection limit; glassy carbon electrode; gold; graphene; nanogold; surface area
Abstract:
... A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase (ChOx) was adsorbed on the surface of AuNPs to construct a cho ...
DOI:
10.1016/j.bios.2011.12.008

32. A high-throughput solid phase screening method for identification of lignocellulose-degrading bacteria from environmental isolates

Author:
Jeffrey G. Gardner; Laura A. Zeitler; Wendy Jo S. Wigstrom; Kristine C. Engel; David H. Keating
Source:
Biotechnology letters 2012 v.34 no.1 pp. 81-89
ISSN:
0141-5492
Subject:
Gram-negative bacteria; acrylamides; agar; biofuels; biotechnology; cellulose; enzymes; hemicellulose; lignocellulose; screening
Abstract:
... The development of cost-effective biofuels will require improvements in the efficiency of biomass deconstruction, a process typically carried out by lignocellulose-degrading enzymes. Environmental microbes represent an abundant and diverse source of lignocelluloses-degrading enzymes for use in biotechnology. However, identification of microorganisms that possess these enzymes has been slowed by a ...
DOI:
10.1007/s10529-011-0742-1
PubMed:
21904949

33. A look at diacylglycerol acyltransferases (DGATs) in algae

Author:
Jit Ern Chen; Alison G. Smith
Source:
Journal of biotechnology 2012 v.162 no.1 pp. 28-39
ISSN:
0168-1656
Subject:
diacylglycerol acyltransferase; biosynthesis; phenotype; bioinformatics; eukaryotic cells; triacylglycerols; biotechnology; biochemical pathways; genes; mammals; biodiesel
Abstract:
... Triacylglycerols (TAGs) from algae are considered to be a potentially viable source of biodiesel and thereby renewable energy, but at the moment very little is known about the biosynthetic pathway in these organisms. Here we compare what is currently known in eukaryotic algal species, in particular the characteristics of algal diacylglycerol acyltransferase (DGAT), the last enzyme of de novo TAG b ...
DOI:
10.1016/j.jbiotec.2012.05.009

34. A low energy process for the recovery of bioproducts from cyanobacteria using a ball mill

Author:
Bangaru Balasundaram; Stephen C. Skill; Carole A. Llewellyn
Source:
Biochemical engineering journal 2012 v.69 no. pp. 48-56
ISSN:
1369-703X
Subject:
Cyanobacteria; biobased products; biomass; bioprocessing; biorefining; biotechnology; energy efficiency; gravity; grinding; microalgae; milling; process energy
Abstract:
... Efficient and economical large scale bioprocessing of microalgae to produce a range of bio-products, working towards a biorefinery approach, is critical for the success of algal industrial biotechnology. The key process variables that affect the recovery of products from different sub-cellular locations were investigated using a high throughput cell disruption system and a cyanobacterium as model ...
DOI:
10.1016/j.bej.2012.08.010

35. A modified and inexpensive protocol for the rapid isolation of RNA from diverse plant species

Author:
Dana Jawdat; Intissar Karajoli
Source:
Journal of horticultural science & biotechnology 2012 v.87 no.4 pp. 317-324
ISSN:
2380-4084
Subject:
Liliopsida; Populus; RNA; analytical kits; barley; biotechnology; borates; cost effectiveness; cotton; filtration; horticulture; markets; perennials; phenolic compounds; plant tissues; potatoes; protocols; recalcitrant species; reverse transcriptase polymerase chain reaction; tissue weight; tobacco; transcriptomics; wheat
Abstract:
... The availability of an inexpensive and rapid procedure for total RNA isolation, specifically from recalcitrant plant species such as cotton, would enhance the efficiency of transcriptomic research. This work describes the successful modification of a total RNA isolation procedure. The modified procedure takes only 150 min to complete, with high yields and a cost reduction of 90% over available com ...
DOI:
10.1080/14620316.2012.11512871

36. A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations

Author:
Afonso P. Basto; João Piedade; Ruben Ramalho; Susana Alves; Helena Soares; Pierre Cornelis; Carlos Martins; Alexandre Leitão
Source:
Journal of biotechnology 2012 v.157 no.1 pp. 50-63
ISSN:
0168-1656
Subject:
African swine fever virus; Escherichia coli; Orf virus; Pseudomonas aeruginosa; adaptive immunity; affinity chromatography; antigens; bacteria; biotechnology; lipoproteins; palmitic acid; receptors; vaccines
Abstract:
... The conjugation of antigens with ligands of pattern recognition receptors (PRR) is emerging as a promising strategy for the modulation of specific immunity. Here, we describe a new Escherichia coli system for the cloning and expression of heterologous antigens in fusion with the OprI lipoprotein, a TLR ligand from the Pseudomonas aeruginosa outer membrane (OM). Analysis of the OprI expressed by th ...
DOI:
10.1016/j.jbiotec.2011.11.006

37. A new method for random mutagenesis by error-prone polymerase chain reaction using heavy water

Author:
Toshifumi Minamoto; Eitaro Wada; Isamu Shimizu
Source:
Journal of biotechnology 2012 v.157 no.1 pp. 71-74
ISSN:
0168-1656
Subject:
Plecoglossus altivelis; actin; biotechnology; complementary DNA; deuterium oxide; fish; genes; honey bees; manganese; mutagenesis; polymerase chain reaction; protein engineering; rhodopsin; solvents; water
Abstract:
... Error-prone polymerase chain reactions (epPCRs) are often used to introduce mutations in random mutagenesis, which has been used as a tool in protein engineering. Here, we developed a new method of epPCR using heavy water as a solvent instead of normal water (H₂O). Rhodopsin cDNA of the Ayu fish (Plecoglossus altivelis) was used as a template and was amplified using five different conditions: (A ...
DOI:
10.1016/j.jbiotec.2011.09.012

38. A new method for the detection and elimination of Hydrangea ringspot virus

Author:
E. K. Tóth; É. Kriston; Á. Máthé; E. Jámbor-benczúr
Source:
Journal of horticultural science & biotechnology 2012 v.87 no.1 pp. 13-16
ISSN:
2380-4084
Subject:
Hydrangea; Hydrangea ringspot virus; biotechnology; horticulture; leaves; meristems; nucleic acids; plant diseases and disorders; plant viruses; reverse transcriptase polymerase chain reaction
Abstract:
... The complete eradication of Hydrangea ringspot virus (HRSV) from Hydrangea by in vitro pre-treatment with ribavirin and meristem tip culture is reported. A sensitive RT-PCR method for the detection of HRSV in newly-formed regenerants was developed. Total nucleic acid extracts from HRSV-infected Hydrangea leaves diluted 10⁵-fold gave a positive reaction using this method. ...
DOI:
10.1080/14620316.2012.11512823

39. A new molecular approach based on qPCR for the quantification of fecal bacteria in contaminated marine sediments

Author:
Gian Marco Luna; Antonio Dell’Anno; Biancamaria Pietrangeli; Roberto Danovaro
Source:
Journal of biotechnology 2012 v.157 no.4 pp. 446-453
ISSN:
0168-1656
Subject:
DNA; Enterococcus; Escherichia coli; Salmonella; bacteria; bioremediation; biotechnology; dredging; marine sediments; monitoring; nutrients; quantitative polymerase chain reaction; ribosomal RNA; risk; sediment contamination; temperature
Abstract:
... Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based on the use of Real Time Quantitative PCR (qPCR), targeting both bacterial DNA and ...
DOI:
10.1016/j.jbiotec.2011.07.033

40. A novel functional assay for fungal histidine kinases group III reveals the role of HAMP domains for fungicide sensitivity

Author:
Anna Buschart; Katja Gremmer; Mohammed El-Mowafy; Joop van den Heuvel; Peter P. Mueller; Ursula Bilitewski
Source:
Journal of biotechnology 2012 v.157 no.1 pp. 268-277
ISSN:
0168-1656
Subject:
Candida albicans; amino acids; antibiotics; antifungal agents; biotechnology; fludioxonil; fungi; genes; growth retardation; histidine kinase; humans; mechanism of action; mitogen-activated protein kinase; mutants; pathogens; phosphorylation; screening; signal transduction
Abstract:
... Signal transduction systems comprising histidine kinases are suggested as new molecular targets of antibiotics. The important human fungal pathogen Candida albicans possesses three histidine kinases, one of which is the type III histidine kinase CaNik1, which activates the MAP kinase Hog1. We established a screening system for inhibitors of this class of histidine kinases by functional expression ...
DOI:
10.1016/j.jbiotec.2011.09.017

41. A novel poly[(N-vinylimidazole)-co-(1-pyrenylmethyl methacrylate)] ferric complex with fluorescence and superparamagnetism

Author:
Jian Cui; Shaobei Yang; Jinfang Zhang; Shuai Zhao; Yehai Yan
Source:
RSC advances 2012 v.2 no.32 pp. 12224-12230
ISSN:
2046-2069
Subject:
biotechnology; coatings; composite polymers; ferric chloride; fluorescence; fluorescent dyes; magnetism; moieties; nanoparticles; physicochemical properties
Abstract:
... Due to its unique physical and chemical properties, the polymer N-vinylimidazole (VI), either as a homopolymer or copolymer, has a large number of important applications. Currently, technological advances have identified further uses for it and there are practical demands for multiply functional forms of the polymer; e.g., fluorescence and magnetism in one VI-polymer material. Toward this end, a n ...
DOI:
10.1039/c2ra22395c

42. A novel strategy to over-express and purify homologous proteins from Streptococcus pneumoniae

Author:
Morena Lo Sapio; Markus Hilleringmann; Michèle A. Barocchi; Monica Moschioni
Source:
Journal of biotechnology 2012 v.157 no.2 pp. 279-286
ISSN:
0168-1656
Subject:
Escherichia coli; Streptococcus pneumoniae; biotechnology; digestion; gene overexpression; mass spectrometry; mutagenesis; mutants; plasmids; protein structure; protein synthesis; proteins; proteolysis; replicon; trypsin; virulence
Abstract:
... Functional studies of Streptococcus pneumoniae virulence factors are facilitated by the development of complementation/mutagenesis systems. These methods usually result in poor expression yields; therefore, biochemical and structural/functional characterizations are mostly performed with proteins expressed and purified from heterologous systems (e.g. Escherichia coli). However, heterologous expres ...
DOI:
10.1016/j.jbiotec.2011.11.011

43. A nutrient medium for diverse applications and tissue growth of plant species in vitro

Author:
Michael B. Greenway; Isaac C. Phillips; Meagan N. Lloyd; John F. Hubstenberger; Gregory C. Phillips
Source:
In vitro cellular & developmental biology 2012 v.48 no.4 pp. 403-410
ISSN:
1054-5476
Subject:
biomass production; biotechnology; calcium chloride; callus; corn; cotton; growth and development; micropropagation; nutrients; onions; plant morphology; production technology; rice; root growth; secondary metabolites; soybeans; tobacco
Abstract:
... The basal salt formulation of a medium is a vital but often overlooked component in many in vitro applications, as it regulates the growth and morphology of plant tissues by providing essential nutrients. The MS and B5 formulations are the two most widely used basal media, yet they are suboptimal for many species. The objective of this study was to evaluate the BDS (modified B5) basal salt formula ...
DOI:
10.1007/s11627-012-9452-1

44. A pomegranate (Punica granatum L.) linkage map based on AFLP markers

Author:
A. Sarkhosh; Z. Zamani; R. Fatahi; C. Wiedow; D. Chagné; S. E. Gardiner
Source:
Journal of horticultural science & biotechnology 2012 v.87 no.1 pp. 1-6
ISSN:
2380-4084
Subject:
Punica granatum; amplified fragment length polymorphism; biotechnology; chromosome mapping; genetic markers; genome; genotype; horticulture; linkage groups; pomegranates; progeny
Abstract:
... A genetic linkage map of pomegranate (Punica granatum L.; 2n = 2x = 16) was constructed using 62 individuals of an F₁ progeny derived from a cross between a commercial pomegranate variety, ‘Malas Danesiyah Esfahani’ (MDE), and ‘Bihaste Danesefid Ravar’ (BDR), a genotype that carries a unique soft-seed character.Twenty-six amplified fragment length polymorphic (AFLP) marker primer combinations yiel ...
DOI:
10.1080/14620316.2012.11512821

45. A possible mechanism for phloem transport of boron in ‘Hass’ avocado (Persea americana Mill.) trees

Author:
P. E. H. Minchin; T. G. Thorp; H. L. Boldingh; N. Gould; J. M. Cooney; F. B. Negm; E. Focht; M.L. Arpaia; H. Hu; P. Brown
Source:
Journal of horticultural science & biotechnology 2012 v.87 no.1 pp. 23-28
ISSN:
2380-4084
Subject:
Persea americana; avocados; biotechnology; boron; fruit set; horticulture; inflorescences; leaves; mannitol; phloem; sap; sorbitol; trees
Abstract:
... A continuous supply of boron (B) is vital for the formation of new cells, with reproductive growth being particularly sensitive to boron deficiency. An inadequate B supply therefore has the potential to effect fruit set, which is a major problem in avocado (Persea americana Mill.) cultivation. B is not phloem mobile in many plant species, but when sorbitol or mannitol (both polyols) are present in ...
DOI:
10.1080/14620316.2012.11512825

46. A qNMR approach for bitterness phenotyping and QTL identification in an F1 apricot progeny

Author:
Claudia Cervellati; Christian Paetz; Luca Dondini; Stefano Tartarini; Daniele Bassi; Bernd Schneider; Andrea Masia
Source:
Journal of biotechnology 2012 v.159 no.4 pp. 312-319
ISSN:
0168-1656
Subject:
amygdalin; animal nutrition; apricots; biotechnology; bitterness; commercialization; crushing; enzymes; genes; humans; hydrogen cyanide; loci; nuclear magnetic resonance spectroscopy; phenotype; progeny; quantitative trait loci; seeds; toxicity; variance
Abstract:
... In apricot the bitter flavor of seeds is determined by the amount of amygdalin, a cyanogenic glucoside whose cleavage by endogenous enzymes, upon seed crushing, releases toxic hydrogen cyanide. The presence of such a poisonous compound is an obstacle to the use and commercialization of apricot seeds for human or animal nutrition. To investigate the genetic loci involved in the determination of the ...
DOI:
10.1016/j.jbiotec.2011.09.004

47. A quantitative framework for the formation of liquid marbles and hollow granules from hydrophobic powders

Author:
Nicky Eshtiaghi; Karen P. Hapgood
Source:
Powder technology 2012 v.223 no. pp. 65-76
ISSN:
0032-5910
Subject:
biotechnology; droplets; glass; glycerol; granules; hydrophobicity; pharmaceutical industry; powders
Abstract:
... Liquid marbles are micro reservoirs of fluid surrounded by a powder shell. Their unique properties show promise for high-value technological applications in the medical, biotechnology, chemical and pharmaceutical industries. In this study, liquid marbles were prepared from six mixtures of water and glycerol by releasing droplets from different heights onto a loosely packed powder bed using a 1mL s ...
DOI:
10.1016/j.powtec.2011.05.007

48. A rapid generation of adenovirus vector with a genetic modification in hexon protein

Author:
Bingyan Di; Qinwen Mao; Junli Zhao; Xing Li; Dongyang Wang; Haibin Xia
Source:
Journal of biotechnology 2012 v.157 no.3 pp. 373-378
ISSN:
0168-1656
Subject:
Adenoviridae; biotechnology; color; gene therapy; genetic engineering; genetic vectors; rapid methods; restriction endonucleases; screening; viruses
Abstract:
... The generation of hexon-modified adenovirus vector has proven difficult. In this paper, we developed a novel method for rapid generation of hexon-modified adenoviral vector via one step ligation in vitro followed by quick white/blue color screening. The new system has the following features. First, eGFP expression driven by the CMV promoter in E1 region functions as a reporter to evaluate the trop ...
DOI:
10.1016/j.jbiotec.2011.12.022

49. A rapid, targeted, neuron-selective, in vivo knockdown following a single intracerebroventricular injection of a novel chemically modified siRNA in the adult rat brain

Author:
Hidemitsu Nakajima; Takeya Kubo; Yuko Semi; Masanori Itakura; Mitsuru Kuwamura; Takeshi Izawa; Yasu-Taka Azuma; Tadayoshi Takeuchi
Source:
Journal of biotechnology 2012 v.157 no.2 pp. 326-333
ISSN:
0168-1656
Subject:
RNA interference; Western blotting; adults; biotechnology; cerebellum; cortex; gene expression; glyceraldehyde-3-phosphate dehydrogenase; hippocampus; immunohistochemistry; inflammation; neurons; proteins; rats; small interfering RNA; toxicity; transfection
Abstract:
... There has been a dramatic expansion of the literature on RNA interference and with it, increasing interest in the potential clinical utility of targeted inhibition of gene expression and associated protein knockdown. However, a critical factor limiting the experimental and therapeutic application of RNA interference is the ability to deliver small interfering RNAs (siRNAs), particularly in the cen ...
DOI:
10.1016/j.jbiotec.2011.10.003

50. A short adaptive path from DNA to RNA polymerases

Author:
Christopher Cozens; Vitor B. Pinheiro; Alexandra Vaisman; Roger Woodgate; Philipp Holliger
Source:
Proceedings of the National Academy of Sciences of the United States of America 2012 v.109 no.21 pp. 8067-8072
ISSN:
0027-8424
Subject:
DNA; DNA-directed DNA polymerase; DNA-directed RNA polymerase; RNA; Thermococcus; biotechnology; genome; mutation; polymers; substrate specificity; thymidine
Abstract:
... DNA polymerase substrate specificity is fundamental to genome integrity and to polymerase applications in biotechnology. In the current paradigm, active site geometry is the main site of specificity control. Here, we describe the discovery of a distinct specificity checkpoint located over 25 Å from the active site in the polymerase thumb subdomain. In Tgo, the replicative DNA polymerase from Therm ...
DOI:
10.1073/pnas.1120964109
PubMed:
22566643
PubMed Central:
PMC3361454