Jump to Main Content
- Ryo, Shintaro; Ishii, Jun; Iguchi, Yusuke; Fukuda, Nobuo; Kondo, Akihiko
- Analytical biochemistry 2012 v.424 no.1 pp. 27-31
- G-protein coupled receptors, etc ; G-proteins; agonists; flow cytometry; galactose; gene induction; genetically engineered microorganisms; green fluorescent protein; ligands; regulator genes; regulon; reporter genes; screening; signal transduction; structural genes; transcription (genetics); yeasts; Show all 17 Subjects
- ... Here we present a successful transplantation of the GAL genetic regulatory circuitry into the G-protein signaling pathway in yeast. The GAL regulon represents a strictly regulated transcriptional mechanism that we have transplanted into yeast to create a highly robust induction system to assist the detection of on–off switching in G-protein signaling. In our system, we engineered yeast to drive th ...