An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
mass spectrometry; polyacrylamide gel electrophoresis; proteins; proteomics
Abstract:
... A sensitive and convenient “visible SYPRO” staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive th ...
Fissidens; bryology; leaves; mosses and liverworts; new species; soil; China; Macau
Abstract:
... Fissidens macaoensis L. Zhang is described as a new species from Macao, South China. The species can be separated from its congeners by a suite of characters, including (1) minute size; (2) subterraneous tubers, composed of one to several cells, growing laterally from mature rhizoids; (3) abundant rhizoidal gemmae found on the surface of the soil and which are developed from subterraneous rhizoids ...
... The rice (Oryza sativa L.) elongated uppermost internode (eui) gene effectively results in a recessive tall plant type. In eui background, we found a mutant 02428ha that exhibited a semidwarf phenotype. Because semidwarf varieties possess high yield potential, this mutant may be useful for hybrid rice breeding. The present study aimed to characterize the dwarfing mechanism in the mutant. Microscop ...
... Subsurface geochemical behavior of As(V) with Fe(II) was studied under strict anoxic conditions. Abiotic reduction of As(V) (0.1 mM) to As(III) by aqueous Fe(II) and sorbed Fe(II) in pH range 5.0-7.0 and Fe(II)aq concentration (0.6-1.2 mM) was investigated along with the effect of As(V) on the oxidation of Fe(II) by dissolved oxygen (DO). Although the reduction was thermodynamically feasible for h ...
... Canola oil is high in oleic acid which is commonly used for food and industrial purposes. To determine adaptability of spring canola (Brassica napus L.) to the High Plains for industrial oil production, 26 irrigated trials were conducted from 2005 to 2008. Trials were divided into five regions—1: 36–37°N 108°W; 2: 39–40°N 101–103°W; 3: 41–42°N 102–103°W; 4: 41–42°N 104°W; 5: 43–44°N 106–108°W. Cul ...
chromium; cobalt; copper; correlation; eutrophication; geochemistry; heavy metals; industrial effluents; industrial wastes; lakes; lead; manganese; mercury; nickel; normal values; nutrients; phosphorus; principal component analysis; rivers; sediments; sewage; strontium; titanium; vanadium; wastewater; zinc; China
Abstract:
... Concentrations of the nutrients (TN and TP), phosphorus fractions and heavy metals (Co, Cr, Cu, Mn, Ni, Pb, Sr, Ti, V, Zn and Hg) in 40 surface sediment samples collected from Taihu Lake, a eutrophic shallow lake in China, were determined. The results showed that the northwest region of the lake possessed higher concentrations of TN and TP, as well as the similar spatial distribution trend in the ...
... A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase K treatment (300 μg ml−1) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free culture fluids f ...
adsorption; biotechnology; breeding; engineering; enzymes; metal ions; pollutants; proteins; yeasts
Abstract:
... Cell surface engineering is a promising strategy for the molecular breeding of whole-cell biocatalysts. By using this strategy, yeasts can be constructed by the cell surface display of functional proteins; these yeasts are referred to as arming yeasts. Because reactions using arming yeasts as whole-cell biocatalysts occur on the cell surface, materials that cannot enter the cell can be used as rea ...
Jasus; coasts; demersal fish; ecosystems; geographical variation; latitude; lobsters; multivariate analysis; population dynamics; surveys; temporal variation; South Africa
Abstract:
... Research survey data collected over 24 years (1986–2009) were used to explore long-term changes in demersal fish assemblages on the west coast of South Africa. Differences in spatial (latitude and depth) and temporal (seasonal and annual) factors were examined using multivariate analyses. Fish assemblages are clearly influenced by depth, with a distinct change in the region of the shelf break betw ...
... The physicochemical characteristics, fatty acid and triacylglycerol compositions, DSC profile and UV/vis spectrum of oil extracted from Albizia julibrissin seeds were determined in this study. The oil content and the moisture of the seeds were 10.50% and 1.56%. The free fatty acid, the peroxide value, the p-anisidine value, the saponification value, the iodine value were 2.54%, 6.61mequiv. O₂/kg o ...
Marchantiopsida; bryology; models; Azores; Portugal
Abstract:
... Conocephalum salebrosum Szweykowski, Buczkowska & Odrzykoski, a hygrophytic species, is newly reported for several localities in continental Portugal as well as in Madeira and Azores Islands. Information from studied specimens, and a map showing the actual distribution of this species and its closely related species, C. conicum (L.) Dumort., are provided as the basis for a discussion on the chorol ...
Saccharomyces cerevisiae; cysteine; mutagenesis; mutants; protein secretion; protein synthesis; screening; serine
Abstract:
... PURPOSE OF WORK: Soluble protein expression is an important first step during various types of protein studies. Here, we present the screening strategy of secretable mutant. The strategy aimed to identify those cysteine residues that provoke protein misfolding in the heterologous expression system. Intentional mutagenesis studies should consider the size of the library and the time required for ex ...
aerators; alum; arsenates; arsenic; arsenites; boats; copper sulfate; eutrophication; lakes; metals; mixing; recreation; sediments; summer; traffic; wind; winter; New Jersey
Abstract:
... Elevated concentrations of arsenic (As) occurred during warm months in water from the outlet of Lake Mohawk in northwestern New Jersey. The shallow manmade lake is surrounded by residential development and used for recreation. Eutrophic conditions are addressed by alum and copper sulfate applications and aerators operating in the summer. In September 2005, arsenite was dominant in hypoxic to anoxi ...
Agoseris; Megaptera novaeangliae; Physeter macrocephalus; Tursiops truncatus; data collection; geophysics; species diversity; spring; surveys; winter; Angola; Gulf of Guinea
Abstract:
... The species richness, spatial distribution, seasonality and interspecific associations of cetaceans in tropical oceanic waters between the Gulf of Guinea and Angola were examined using 5 905.3 h of dedicated survey effort collected from 13 platforms of opportunity (geophysical vessels) between 2004 and 2009, and from incidental records. Most effort (87.8%) was recorded in waters >1 000 m deep. A t ...
Salvia miltiorrhiza; root shoot ratio; roots; rosmarinic acid; salvianolic acid; semiarid zones; shoots; traditional medicine; water stress
Abstract:
... The roots of Salvia miltiorrhiza Bunge are widely used in traditional Chinese medicine. The objective of this study was to investigate the effect of drought stress on growth and active constituents composition in S. miltiorrhiza. Three water-stress treatments, including control, medium drought stress and severe drought stress, were applied on the whole growth cycle. The results showed that drought ...
... Dyes derived from natural sources have emerged as an important alternative to synthetic dyes. Therefore, there is a need for developing better solidâliquid extraction techniques for leaching natural colorants from plant materials for applications in plant research, food as well as dyeing industries. The influence of ultrasound on natural colorant extraction from different potential dye yielding ...
... The objectives of the study were to evaluate the effects of defoliation on tiller growth of Poa ligularis Ness. at two different intervals after fire as well as how recovery is affected by different levels of herbaceous interspecific competition during the subsequent growing season. Early post-fire defoliation (6 months after burning) reduced (P<0.05) the height, number of green leaves and total g ...
broiler chickens; veterinary drugs; antibiotics; surveys; animal pathogens; broiler feeding; costs and returns; United States
Abstract:
... We use data from a recent national survey to analyze the use of sub-therapeutic antibiotics (STAs) among broiler growers. We estimate that 42% of growers do not use STAs in their feed or water, and instead rely on a set of other practices, including pathogen testing, expanded sanitary protocols, altered feeding regimens, and Hazard Analysis and Critical Control Point HACCP plans to maintain produc ...
... Catches of sharks and bycatch in large-mesh nets and baited drumlines used by the Queensland Shark Control Program were examined to determine the efficacy of both gear types and assess fishing strategies that minimise their impacts. There were few significant differences in the size of both sharks and bycatch in the two gear types, apart from significantly smaller (p < 0.05) tiger sharks Galeocerd ...
... Whether occurrence of life at the physicochemical extremes results from the entire adaptation of organisms to such settings or it originates from the action of a few genes has been debated for a long time. Recent evidence suggests that a limited number of functions suffice to change the predilection of microorganisms for radically different environmental scenarios. For instance, expression of a fe ...
... The environmental impacts of Boroo gold mine project in Mongolia was evaluated by chemical characterization of trace element concentrations in water, soils and tailing dam sediment samples. The results showed that concentrations of B, Cd, Ni and Se in the water samples were within the accepted levels of the Mongolia water quality standard (MNS4586: 1998). However, the concentrations of Al, As, Cu, ...
anthropogenic activities; arsenic; basins; climatic factors; dissolved oxygen; groundwater; heavy metals; nickel; pH; parent rock; river water; rivers; sediments; water temperature; zinc; China
Abstract:
... The Yarlung Tsangbo (YT) is a large river running across southern Tibet and has significant effects on its lower reaches, the Ganges-Brahmaputra Basin. In order to investigate the geochemical features of the YT, 18 surface sediment samples were collected; ten trace element concentrations were measured for bulk sediments and two fine grain size fractions. Meanwhile, basic physicochemical parameters ...
... Elevated foliar concentrations of glutathione (GSH) are a common stress response and potentially crucial in conferring increased stress tolerance. The present study addressed the following questions: can increased foliar GSH levels be achieved in the short term by applying a stem feeding technique to tree seedlings? If yes, will elevated GSH concentrations provide improved tolerance to the adverse ...
... 293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially ...
dogs; elbows; forelimbs; lameness; musculoskeletal diseases; nervous system diseases; shoulders
Abstract:
... Lameness associated with musculoskeletal disorders of the forelimb accounts for approximately 25 per cent of the authors' canine referral caseload. Lameness due to an underlying neurological disorder is less common and is often associated with weakness and, in some dogs, neurological deficits. Most cases of chronic forelimb lameness of musculoskeletal origin seen at the authors' clinic involve the ...
Agave; Fourier transform infrared spectroscopy; adsorption; chemical composition; energy; flax; gas chromatography; hemp; hybrids; kenaf; leaves; pineapples; scanning electron microscopes; sisal; specific energy; zein
Abstract:
... Inverse gas chromatography (IGC) is a suitable method to determine surface energy of natural fibres when compared to wetting techniques. In the present study, the surface properties of raw and modified lignocellulosic fibres have been investigated by IGC. The fibres chosen for the study were flax, hemp, kenaf, agave, agave hybrid, sisal and pineapple. The treatments used were 4% NaOH and 2% zein. ...
Carcharhinus limbatus; coasts; fatty liver; liver; oils; polyunsaturated fatty acids; sharks; Atlantic Ocean; Gulf of Mexico; Indian Ocean; South Africa
Abstract:
... Shark liver oils are rich in polyunsaturated fatty acids, especially the n3 moieties. Data on the liver fatty acids of sharks from African waters, however, are limited. Liver samples from sharks from the western Indian Ocean off the east coast of South Africa and those from the Atlantic Ocean and Gulf of Mexico were examined. This study reports on the fatty acids found in the total lipid fractions ...
... To determine the structural requirements of arenicin-1 in exerting antifungal activity, a truncated peptide with an N-terminal deletion and a peptide with an Ala substitution for an Arg in the beta-turn region were characterised by comparison to arenicin-1. The antifungal activities of the analogues were 25–50% lower than arenicin-1. Trp fluorescence and circular dichroism spectroscopy showed that ...
Chinese hamsters; animal ovaries; cell engineering; gene expression; mechanism of action; microRNA; phenotype
Abstract:
... The ability of microRNAs to influence gene expression is now recognized as a fundamental layer of regulation within the cell. MicroRNAs have a major impact on most biological processes and have generated considerable interest as potential biomarkers as well as therapeutic or engineering targets. In this review we provide a brief overview of their biogenesis, genomic organization and mode of action ...
... This study explores the usefulness of a frame-based modelling approach in the southern Benguela upwelling ecosystem, with four frames describing observed small pelagic fish dominance patterns. We modelled the dynamics of sardine Sardinops sagax and anchovy Engraulis encrasicolus populations under the influence of the small pelagic fishery and climate variability, performed extensive sensitivity an ...
... Farnesyl diphosphate synthase (FPS; EC 2.5.1.1/EC 2.5.1.10) catalyzes the synthesis of farnesyl diphosphate, a key intermediate in the biosynthesis of sesquiterpenes. This present study described the cloning and characterization of a cDNA encoding FPS from leaves of Michelia chapensis Dandy (designated as McFPS, GenBank accession number: GQ214406) for the first time. McFPS was 1,432 bp and contain ...
... The yeast proteins, Msb3p and Msb4p, are two Ypt/Rab-specific GTPase-activating proteins sharing redundant functions in exocytosis, organization of the actin cytoskeleton, and budding site selection. To see if Msb3p might play an additional, specific role, we first tested the sensitivities of msb3 and msb4 mutant strains to different drugs and then screened a genomic library for multicopy suppress ...
databases; early development; females; males; photographs; whales; Mediterranean Sea
Abstract:
... Errors in analysis using natural marks have been recognised since the early development of mark-recapture techniques. In this nine-year study, the mark types present on Cuvier's beaked whales Ziphius cavirostris in the Mediterranean Sea were categorised in order to assess the reliability of natural marks for long-term photo-identification studies. Mark prevalence, abundance and gain/loss rates amo ...
... Biodegradable polymers, such as poly(lactic acid) (PLA) have attracted a lot of attention in the scientific community recently due to a rapid growth of intensive interest in the global environment for alternatives to petroleum-based polymeric materials. Fatty nitrogen compounds (FNCs), fatty amides (FA), fatty hydroxamic acids (FHA), and carbonyl difatty amides (CDFA), which were synthesized from ...
... Recent genome sequencing of Cellvibrio japonicas revealed the presence of two highly homologous ferulic acid esterases (FAEs), encoded by fee1A and fee1B. In this work, the putative FAE, Fee1B, was successfully cloned and expressed in an E. coli system and the purified enzyme was characterized as a type-D FAE with a pH and temperature optima of 6.5 and 35−40°C, respectively. Additionally, the two ...
... Fine fescues are highly valued cool-season turf species. Nearly all of the U.S. fine fescue seed is produced in Oregon, although little is known about the occurrence of weed seed contaminants in fine fescue seed lots. This study was conducted to assess the diversity and frequency of occurrence of weed seed in chewings [Festuca rubra L. subsp. commutata Gaudin], red [Festuca rubra L. subsp. rubra], ...
... Twenty first century has witnessed remarkable achievements in green technology in material science through the development of biocomposites. Oil palm fiber (OPF) extracted from the empty fruit bunches is proven as a good raw material for biocomposites. The cellulose content of OPF is in the range of 43%–65% and lignin content is in the range of 13%–25%. A compilation of the morphology, chemical co ...
... A supply of oxygen, both for the maintenance of anaesthesia using volatile anaesthetic agents and the support of animals requiring additional oxygen, is an essential part of modern veterinary practice. In most practices, this is provided via oxygen cylinders. In this article, Keith Barrand discusses an alternative – oxygen concentrators – with comparisons to cylinder oxygen use, where appropriate. ...
arteries; brain; central nervous system diseases; cysteine; disease models; genes; mice; mutation; myocytes; pathogenesis; smooth muscle; transgenic animals
Abstract:
... Small vessel diseases (SVDs) of the brain are the leading cause of vascular cognitive impairment and a major contributor to stroke in the human adult, however, their pathogenesis is poorly understood. Dominant mutations in NOTCH3 cause CADASIL, one of the most prevalent inherited cerebral SVDs. The disease gene encodes a transmembrane receptor primarily expressed in smooth muscle cells of small ar ...
Perlidae; insect eggs; new species; Arctic region; Indiana
Abstract:
... Perlesta ouabache sp. n. is described from Indiana, USA. Perlesta ouabache is distinguished from Nearctic congeners by characteristics of the male paraprocts and eggs. ...
Digitaria ciliaris; Setaria viridis; acclimation; antioxidant activity; drought; drought tolerance; gas exchange; irrigation; leaves; lipid peroxidation; membrane permeability; peroxidase; photosynthesis; physiological response; plant adaptation; proline; proteins; soil water content; stomatal conductance; superoxide dismutase; water content; water use efficiency
Abstract:
... To understand physiological acclimation of psammophyte to repeated soil drought and rewatering, two psammophytes (Setaria viridis and Digitaria ciliaris) were subjected to three cycles of soil drought and rewatering. The response process of leaf relative water content (RWC), membrane permeability, lipid peroxidation, gas exchange characteristics, antioxidant enzymes, soluble protein, and free prol ...
... The 1-aminocyclopropane-1-carboxylate oxidase gene (ACO1) was upregulated in rice (Oryza sativa L.) phyAphyBphyC mutants lacking any phytochrome and containing the GCC box element, a binding site for rice ethylene-responsive element binding protein 1 (OsEREBP1), in its promoter region. Since the OsEREBP1-like gene EBL1 (OsEREBP1-LIKE 1) was significantly downregulated in phyAphyBphyC mutants, EBL1 ...
... A pilot-scale plant consisting of an oxidation basin (OB), a neutralization basin (NB), a reaction basin (RB), and a settling basin (SB) was designed and built to conduct pilot-scale experiments. With this system, the effects of aeration and pH on ferrous oxidation and on precipitation of the oxidized products were studied systemically. The results of pilot-scale tests showed that aeration at 300 ...
... Stem rust, caused by Puccinia graminis, is a disease of cereal crops worldwide. In Canada, it is primarily controlled using resistant cultivars. Tracking the virulence structure in the pathogen populations is essential to detect new virulent races that may occur. Surveys of barley (Hordeum vulgare), oat (Avena sativa) and wheat (Triticum aestivum) fields and trap nurseries were conducted to provid ...
United States Environmental Protection Agency; arsenic; cadmium; copper; heavy metals; hydrochloric acid; ionic strength; leaching; lead; mine tailings; mining; nickel; soil; solubility; toxicity; zinc; Korean Peninsula
Abstract:
... This study focused on the evaluation of leaching behaviours for arsenic and heavy metals (Cd, Cu, Ni, Pb and Zn) in soils and tailings contaminated by mining activities. Ten representative mine soils were taken at four representative metal mines in Korea. To evaluate the leaching characteristics of the samples, eight extraction methods were adapted namely 0.1 M HCl, 0.5 M HCl, 1.0 M HCl, 3.0 M HCl ...
adsorbents; adsorption; aluminum oxide; arsenates; arsenic; case studies; ferric hydroxide; ferric oxide; filtration; groundwater; pH; titanium dioxide; New Jersey
Abstract:
... Laboratory and field filtration experiments were conducted to study the effectiveness of As(V) removal for five types of adsorbent media. The media included activated alumina (AA), modified activated alumina (MAA), granular ferric hydroxide (GFH), granular ferric oxide (GFO), and granular titanium dioxide (TiO₂). In laboratory batch and column experiments, the synthetic challenge water was used to ...
... Phytoextraction is a remediation technology with a promising application for removing arsenic (As) from soils and waters. Several plant species were evaluated for their As accumulation capacity in hydroponic culture amended with As. Cucumis sativus (cucumber) displayed the highest tolerance against As among 4 plants tested in this study (corn, wheat, sorghum and cucumber). The germination ratio of ...
autumn; physicochemical properties; environmental factors; gelatinization temperature; seasonal variation; thallus; melting point; agar; Gracilaria; Western Australia
Abstract:
... Seasonality in yield, physical and chemical properties of the native agar from different life stages of Gracilaria cliftonii was investigated over a period of six seasons (autumn 2008–winter 2009). Agar yield and its properties varied as a function of seasons and life stages but there was no significant correlation between environmental factors and agar properties (p > 0.05). Native agar varied fr ...
arsenic; cadmium; cement; copper; durability; gold; guidelines; hazardous waste; heavy metals; leaching; lead; mine tailings; mixing; remediation; silver; slags; solidification; toxic substances; waste management; water content; zinc; Korean Peninsula
Abstract:
... The objective of this study is to examine the solidification of toxic elements in tailings by the use of cement and blast furnace slag. Tailings samples were taken at an Au-Ag mine in Korea. To examine the best mixing ratio of tailings and the mixture of ordinary Portland cement (OPC) and blast furnace slag (SG) of 5:5, 6:6, 7:3, and 8:2, the 7:3 ratio of tailings and OPC+SG was adapted. In additi ...
... A stabilization/solidification (S/S) process was used to immobilize Cu in contaminated soils obtained from two army firing ranges sites (A and B) with total Cu concentrations of 520 and 380 mg/kg, respectively. Both waste oyster shells (WOS) and pretreated oyster shells (POS) were used to immobilize Cu in the contaminated soils. Waste oyster shells passing the #10 mesh and #20 mesh were used for t ...