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- Nakayasu, Chihaya, et al. Show all 4 Authors
- Fish & shellfish immunology 2011 v.31 no.4 pp. 524-532
- Edwardsiella tarda; Paralichthys olivaceus; Viral hemorrhagic septicemia virus; bacterial infections; cDNA libraries; chemokines; clones; complementary DNA; flounder; gene expression; gene expression regulation; genes; interferons; leukocytes; messenger RNA; microarray technology; pathogens; response elements; suppression subtractive hybridization; transcription (genetics)
- ... Transcriptional changes in the peripheral blood leucocytes (PBL) of Japanese flounder Paralichthys olivaceus challenged by Edwardsiella tarda and viral hemorrhagic septicemia virus (VHSV) were investigated using suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis. First, we constructed an SSH cDNA library using mRNA samples isolated from PBL of P. olivaceus that had b ...