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1. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate

Author:
Darvishi, Farshad; Destain, Jacqueline; Nahvi, Iraj; Thonart, Philippe; Zarkesh-Esfahani, Hamid
Source:
New biotechnology 2011 v.28 no.6 pp. 756-760
ISSN:
1871-6784
Subject:
recombinant DNA,   etc   ; Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; selective media; sequence analysis; temperature; value-added products; yeasts;     Show all 19 Subjects
Abstract:
... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...
DOI:
10.1016/j.nbt.2011.02.002