Subject: "recombinant DNA" / Publication Year: 2011 / Subject: genes / Text Availability: Citation in PubAg

Author:: Fu, Xiao H.; Wang, Lei; Li, Meng N.; Zeng, Xiao F.; Le, Yi Q.
Source:: Journal of environmental science and health 2011 v.46 no.14 pp. 1640-1647
ISSN:: 1532-4117
Subject:: recombinant DNA, etc ; EDTA (chelating agent); aquatic environment; genes; ionic strength; pH; plasmids; pollution; risk; sodium chloride; Show all 10 Subjects
Abstract:: ... It has been shown that thermal-treatment at 100°C can denature deoxyribonucleic acid (DNA), yet this does not cause it to break down completely. To clarify the risk of gene pollution from thermal-treated recombinant DNA, the renaturation characteristics of thermal-denatured plasmid pET-28b and its persistence in aquatic environments were investigated. The results revealed that the double-stranded ...
DOI:: 10.1080/10934529.2011.623940
PubMed:: 22092240
: http://dx.doi.org/10.1080/10934529.2011.623940

Author:: Alfasi, Sara; Sevastsyanovich, Yanina; Zaffaroni, Lenny; Griffiths, Lesley; Hall, Richard; Cole, Jeffrey
Source:: Journal of biotechnology 2011 v.156 no.1 pp. 11-21
ISSN:: 0168-1656
Subject:: recombinant DNA, etc ; DNA-directed RNA polymerase; Escherichia coli; agar; cytochrome c; cytoplasm; flow cytometry; fluorescence; gene expression; genes; growth retardation; host strains; hosts; mutants; mutation; outer membrane proteins; peroxidase; protein synthesis; recombinant proteins; Show all 19 Subjects
Abstract:: ... High level expression of a recombinant gene results in growth arrest, followed by overgrowth by non-productive derivatives. Two methods are described for the isolation of E. coli BL21* strains that are improved hosts for recombinant protein production. Both are based upon the observations (i) that fluorescence of a C-terminal GFP tag is a reliable reporter of the production and correct folding of ...
DOI:: 10.1016/j.jbiotec.2011.08.016
PubMed:: 21875628
: http://dx.doi.org/10.1016/j.jbiotec.2011.08.016

Author:: Darvishi, Farshad; Destain, Jacqueline; Nahvi, Iraj; Thonart, Philippe; Zarkesh-Esfahani, Hamid
Source:: New biotechnology 2011 v.28 no.6 pp. 756-760
ISSN:: 1871-6784
Subject:: recombinant DNA, etc ; Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; selective media; sequence analysis; temperature; value-added products; yeasts; Show all 19 Subjects
Abstract:: ... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...
DOI:: 10.1016/j.nbt.2011.02.002
: http://dx.doi.org/10.1016/j.nbt.2011.02.002

Author:: Mallikarjuna, Nalini; Senapathy, Senthilvel; Jadhav, Deepak R.; Saxena, Kulbhushan; Sharma, Hari C.; Upadhyaya, Hari D.; Rathore, Abishek; Varshney, Rajeev
Source:: Plant breeding 2011 v.130 no.5 pp. 507-514
ISSN:: 0179-9541
Subject:: recombinant DNA, etc ; Bruchidae; Cajanus cajan; Phytophthora; backcrossing; blight; color; cytoplasmic male sterility; disease resistance; genes; genetic background; genetic improvement; genetic recombination; growth habit; interspecific hybridization; nucleotide sequences; parents; pest resistance; pigeon peas; plant breeding; pollination; progeny; seeds; tissue culture; wild relatives; Show all 25 Subjects
Abstract:: ... With 1 figure and 4 tables ABSTRACT: Cultivated pigeonpea (Cajanus cajan L. Millsp.) has a narrow genetic base; hence, utilization of wild relatives in the crossing programme would broaden its genetic base and introduce useful traits. Cajanus platycarpus (Benth.) Maesen, an annual wild relative from the tertiary gene pool, was successfully crossed with the cultigen, utilizing hormone‐aided pollina ...
DOI:: 10.1111/j.1439-0523.2011.01870.x
: http://dx.doi.org/10.1111/j.1439-0523.2011.01870.x

Author:: Moeini, Hassan; Omar, Abdul Rahman; Rahim, Raha Abdul; Yusoff, Khatijah
Source:: Comparative immunology, microbiology, and infectious diseases 2011 v.34 no.3 pp. 227-236
ISSN:: 0147-9571
Subject:: recombinant DNA, etc ; Chicken anemia virus; anemia; blood serum; chickens; genes; in vivo studies; interferon-gamma; interleukin-2; neutralizing antibodies; plasmids; proteins; recombinant vaccines; splenocytes; Show all 14 Subjects
Abstract:: ... In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were ...
DOI:: 10.1016/j.cimid.2010.11.006
: http://dx.doi.org/10.1016/j.cimid.2010.11.006

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