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- Yoshikawa, Masahide, et al. Show all 10 Authors
- Journal of bioscience and bioengineering 2011 v.111 no.1 pp. 71-77
- coculture; embryonic stem cells; leukemia inhibitory factor; messenger RNA; mice; prealbumin; rats; reverse transcriptase polymerase chain reaction; tryptophan 2,3-dioxygenase
- ... We investigated the effects of coculture with hepatic stellate cells (HSCs) on the differentiation of embryonic stem (ES) cells and embryoid bodies (EBs). Rat HSCs were incubated until becoming semi-confluent and adherent to the dish. Undifferentiated mouse ES cells and 4-day EBs were cultured in gelatin-coated or HSC-feeder dishes, then induced hepatocyte-like cells and the remaining undifferenti ...