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- Aiken, Jane T.
- Soil Science Society of America journal 2011 v.75 no.1 pp. 102-111
- community ecology, etc ; soil microorganisms; species diversity; microbial genetics; restriction fragment length polymorphism; genetic markers; ribosomal RNA; nucleotide sequences; methodology; laboratory techniques; DNA fingerprinting; data analysis; accuracy; Show all 13 Subjects
- ... Terminal restriction fragment length polymorphism (TRFLP) fingerprinting of 16S rRNA genes is a popular technique for analyzing bacterial communities. This review considers the technical aspects of deriving a community fingerprint with the goal of improving TRFLP as a tool for community ecologists, particularly those working in soil science. Analysis parameters are reviewed for samples run with Ge ...