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"recombinant DNA"
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2011
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- Author:
- Pinyachat, Anuwat; Rojnuckarin, Ponlapat; Muanpasitporn, Chuanchom; Singhamatr, Pon; Nuchprayoon, Surang
- Source:
- Toxicon 2011 v.57 no.5 pp. 772-780
- ISSN:
- 0041-0101
- Subject:
- recombinant DNA, etc ; Komagataella pastoris; sequence alignment; metalloproteinases; polyacrylamide gel electrophoresis; Western blotting; antivenoms; inhibitory concentration 50; basement membrane; venoms; placenta; signal peptide; patients; pathogenesis; cysteine; complementary DNA; humans; collagen; platelet aggregation; molecular cloning; therapeutics; snakes; Show all 22 Subjects
- Abstract:
- ... Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using re ...
- DOI:
- 10.1016/j.toxicon.2011.02.011
- http://dx.doi.org/10.1016/j.toxicon.2011.02.011
- Author:
- Wiker, Harald G.; Tomazella, Gisele G.; de Souza, Gustavo A.
- Source:
- Journal of proteomics 2011 v.74 no.9 pp. 1711-1719
- ISSN:
- 1874-3919
- Subject:
- recombinant DNA, etc ; DNA libraries; Mycobacterium leprae; antigens; bioinformatics; genomics; immune response; leprosy; pathogens; prediction; proteins; proteomics; pseudogenes; virulence; Show all 14 Subjects
- Abstract:
- ... Leprosy is an ancient disease and the focus of the researchers' scrutiny for more than a century. However, many of the molecular aspects related to transmission, virulence, antigens and immune responses are far from known. Initially, the implementation of recombinant DNA library screens raised interesting antigen candidates. Finally, the availability of Mycobacterium leprae genomic information sho ...
- DOI:
- 10.1016/j.jprot.2011.01.004
- PubMed:
- 21278007
- http://dx.doi.org/10.1016/j.jprot.2011.01.004
- Author:
- Waiblinger, Hans-Ulrich; Graf, Norbert; Broll, Hermann; Grohmann, Lutz; Pietsch, Klaus
- Source:
- Journal fuÌr Verbraucherschutz und Lebensmittelsicherheit = 2011 v.6 no.4 pp. 411-417
- ISSN:
- 1661-5751
- Subject:
- recombinant DNA, etc ; detection limit; genetically modified organisms; quality control; quantitative polymerase chain reaction; Show all 5 Subjects
- Abstract:
- ... Real-time polymerase chain reaction (real-time PCR) facilitates to detect DNA fragments at very low copy numbers. Positive results, e.g. for unauthorized genetically modified organism (GMO) contamination or for allergens, may raise safety concerns and have far-reaching consequences. However, in case of very low concentrations of DNA samples, results for the same product lot or even for identical s ...
- DOI:
- 10.1007/s00003-011-0669-4
- http://dx.doi.org/10.1007/s00003-011-0669-4
- Author:
- Knezevic, Ivana; Griffiths, Elwyn
- Source:
- Biologicals 2011 v.39 no.5 pp. 252-255
- ISSN:
- 1045-1056
- Subject:
- recombinant DNA, etc ; biological production; biopharmaceutical industry; biopharmaceuticals; information exchange; interferons; markets; patents; patient care; quality of life; somatotropin; Show all 11 Subjects
- Abstract:
- ... Biotechnology derived medicinal products are presently the best characterized biologicals with considerable production and clinical experience, and have revolutionized the treatment of some of the most difficult-to-treat diseases, prolonging and improving the quality of life and patient care. They are also currently one of the fastest growing segments of the pharmaceutical industry market. The cri ...
- DOI:
- 10.1016/j.biologicals.2011.09.005
- http://dx.doi.org/10.1016/j.biologicals.2011.09.005
- Author:
- Nebija, Dashnor; Urban, Ernst; Stessl, Martina; Noe, Christian R.; Lachmann, Bodo
- Source:
- Electrophoresis 2011 v.32 no.12 pp. 1438-1443
- ISSN:
- 0173-0835
- Subject:
- recombinant DNA, etc ; matrix-assisted laser desorption-ionization mass spectrometry; polyacrylamide gel electrophoresis; post-translational modification; recombinant proteins; Show all 5 Subjects
- Abstract:
- ... 2-DE and MALDI-TOF MS are useful techniques for the quality evaluation of medicinal products derived from recombinant DNA technology. The principal objective of this study has been to evaluate the suitability of 2-DE in combination with MALDI-TOF MS for the quality study of the therapeutic recombinant protein, abatacept. 1-DE SDS-PAGE, under reducing and nonreducing conditions, and 2-DE analysis w ...
- DOI:
- 10.1002/elps.201100029
- http://dx.doi.org/10.1002/elps.201100029
- Author:
- Moeini, Hassan; Omar, Abdul Rahman; Rahim, Raha Abdul; Yusoff, Khatijah
- Source:
- Comparative immunology, microbiology, and infectious diseases 2011 v.34 no.3 pp. 227-236
- ISSN:
- 0147-9571
- Subject:
- recombinant DNA, etc ; Chicken anemia virus; anemia; blood serum; chickens; genes; in vivo studies; interferon-gamma; interleukin-2; neutralizing antibodies; plasmids; proteins; recombinant vaccines; splenocytes; Show all 14 Subjects
- Abstract:
- ... In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were ...
- DOI:
- 10.1016/j.cimid.2010.11.006
- http://dx.doi.org/10.1016/j.cimid.2010.11.006
- Author:
- Egawa, Edgar Y.; Kato, Koichi; Hiraoka, Makiko; Nakaji-Hirabayashi, Tadashi; Iwata, Hiroo
- Source:
- Biomaterials 2011 v.32 no.21 pp. 4737-4743
- ISSN:
- 0142-9612
- Subject:
- recombinant DNA, etc ; Parkinson disease; astrocytes; brain; cell proliferation; cell transplantation; collagen; epidermal growth factor; gene expression; hydrocolloids; neurons; polypeptides; rats; stem cells; Show all 14 Subjects
- Abstract:
- ... Neural stem cells (NSCs) have received much attention in cell-transplantation therapy for central nervous disorders such as Parkinson’s disease. However, poor engraftment of transplanted cells limits the efficacy of the treatments. To overcome this problem, collagen-based hydrogels were designed in this study to provide microenvironments for embedded cells to survive and proliferate. Our approach ...
- DOI:
- 10.1016/j.biomaterials.2011.03.033
- http://dx.doi.org/10.1016/j.biomaterials.2011.03.033
8. Evaluation of similar biotherapeutic products (SBPs): Scientific principles and their implementation
- Author:
- Knezevic, Ivana
- Source:
- Biologicals 2011 v.39 no.5 pp. 256-261
- ISSN:
- 1045-1056
- Subject:
- recombinant DNA, etc ; World Health Organization; chronic diseases; guidelines; markets; patents; patients; prices; public health; therapeutics; Show all 10 Subjects
- Abstract:
- ... The availability of biotherapeutic products to patients has a major impact on the success in treating many life-threatening and chronic diseases. These products are often derived by recombinant DNA technology and are expensive for the majority of patients who need them most. Increasing numbers of patents/data protection are now expiring and biologicals “similar” to the originators (innovative prod ...
- DOI:
- 10.1016/j.biologicals.2011.08.008
- http://dx.doi.org/10.1016/j.biologicals.2011.08.008
- Author:
- Tovkach, Andriy; Zeevi, Vardit; Tzfira, Tzvi
- Source:
- Journal of biotechnology 2011 v.151 no.1 pp. 1-8
- ISSN:
- 0168-1656
- Subject:
- recombinant DNA, etc ; biotechnology; engineering; molecular cloning; nucleotide sequences; purification methods; restriction endonucleases; zinc finger motif; Show all 8 Subjects
- Abstract:
- ... The limited number of naturally occurring rare-cutting restriction enzymes and the slow and tedious engineering of existing restriction enzymes for novel specificities have prompted the design of new strategies for the development of restriction enzymes with specificities for long DNA sequences. One possibility is using zinc finger nucleases (ZFNs)âsynthetic restriction enzymes that are custom-d ...
- DOI:
- 10.1016/j.jbiotec.2010.10.071
- http://dx.doi.org/10.1016/j.jbiotec.2010.10.071
- Author:
- Darvishi, Farshad; Destain, Jacqueline; Nahvi, Iraj; Thonart, Philippe; Zarkesh-Esfahani, Hamid
- Source:
- New biotechnology 2011 v.28 no.6 pp. 756-760
- ISSN:
- 1871-6784
- Subject:
- recombinant DNA, etc ; Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; selective media; sequence analysis; temperature; value-added products; yeasts; Show all 19 Subjects
- Abstract:
- ... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...
- DOI:
- 10.1016/j.nbt.2011.02.002
- http://dx.doi.org/10.1016/j.nbt.2011.02.002
11. Identification of a polymeric β-cyclodextrin-binding peptide from a phage-displayed peptide library
- Author:
- Kang, Boram; Choi, Suk-Jung
- Source:
- Analytical biochemistry 2011 v.415 no.1 pp. 46-51
- ISSN:
- 0003-2697
- Subject:
- recombinant DNA, etc ; Hepatitis C virus; antibody detection; beta-cyclodextrin; binding capacity; detection limit; dissociation; immunoassays; peptide libraries; proteins; Show all 10 Subjects
- Abstract:
- ... In this work, a phage-displayed peptide library was applied to identification of β-cyclodextrin (CD)-binding peptide tag, capable of being combined to target peptides or proteins in a homogeneous way by established methods such as peptide synthesis and the recombinant DNA technique. Four enriched sequences were obtained after five rounds of biopanning against polymeric β-CD beads. One of the seque ...
- DOI:
- 10.1016/j.ab.2011.04.016
- http://dx.doi.org/10.1016/j.ab.2011.04.016
- Author:
- Krupa, Magdalena; Canamero, Marta; Gomez, Carmen E.; Najera, Jose L.; Gil, Jesus; Esteban, Mariano
- Source:
- Vaccine 2011 v.29 no.7 pp. 1504-1513
- ISSN:
- 0264-410X
- Subject:
- recombinant DNA, etc ; T-lymphocytes; Vaccinia virus; adverse effects; antigens; disease control; disease course; histopathology; mice; models; prostatic neoplasms; secondary immunization; vaccination; vaccines; Show all 14 Subjects
- Abstract:
- ... Despite recent advances in early detection and improvement of conventional therapies, there is an urgent need for development of additional approaches for prevention and/or treatment of prostate cancer, and the use of immunotherapeutic modalities, such as cancer vaccines, is one of the most promising strategies. In this study, we evaluated the prophylactic efficacy of an active immunization protoc ...
- DOI:
- 10.1016/j.vaccine.2010.12.016
- http://dx.doi.org/10.1016/j.vaccine.2010.12.016
- Author:
- Ponchon, Luc; Dardel, Frédéric
- Source:
- Methods 2011 v.54 no.2 pp. 267-273
- ISSN:
- 1046-2023
- Subject:
- recombinant DNA, etc ; Escherichia coli; RNA; nucleotide motifs; Show all 4 Subjects
- Abstract:
- ... Stable, folded RNA are involved in many key cellular processes and can be used as tools for biological, pharmacological and/or molecular design studies. However, their widespread use has been somewhat limited by their fragile nature and by the difficulties associated with their production on a large scale, which were limited to in vitro methods. This work reviews the novel techniques recently deve ...
- DOI:
- 10.1016/j.ymeth.2011.02.007
- http://dx.doi.org/10.1016/j.ymeth.2011.02.007
- Author:
- Puigdomenech, P.
- Source:
- Acta horticulturae 2011 no.916 pp. 25-29
- ISSN:
- 0567-7572
- Subject:
- recombinant DNA, etc ; agronomic traits; cultivars; domestication; marker-assisted selection; Show all 5 Subjects
- Abstract:
- ... The origin and the progress of agriculture have relied since its beginning in the Neolithic era in the application of the best available knowledge. Plant domestication involved the identification of a limited number of plant and animal species and a narrow range of variation within the species that allowed their cultivation. Starting at the end of 18th century the recognition of how important agro ...
- Author:
- Misaki, Ryo; Sakai, Yohei; Omasa, Takeshi; Fujiyama, Kazuhito; Seki, Tatsuji
- Source:
- Journal of bioscience and bioengineering 2011 v.112 no.5 pp. 476-484
- ISSN:
- 1389-1723
- Subject:
- recombinant DNA, etc ; biopharmaceuticals; desorption; glycosylation; high performance liquid chromatography; humans; ionization; matrix-assisted laser desorption-ionization mass spectrometry; mice; monoclonal antibodies; polysaccharides; recombinant proteins; signal peptide; sporamin; sugars; tobacco; vacuoles; Show all 17 Subjects
- Abstract:
- ... Recombinant DNA technology enables the use of plants as the host for the production of pharmaceutical proteins, such as antibodies. The glycosylation of recombinant proteins plays physiological and biological roles. However, because glycosylation in plants is different from that in human cells, the development of glycoengineering is required. In plant cells, glycan structures are shown to correlat ...
- DOI:
- 10.1016/j.jbiosc.2011.07.002
- http://dx.doi.org/10.1016/j.jbiosc.2011.07.002
- Author:
- Fu, Xiao H.; Wang, Lei; Li, Meng N.; Zeng, Xiao F.; Le, Yi Q.
- Source:
- Journal of environmental science and health 2011 v.46 no.14 pp. 1640-1647
- ISSN:
- 1532-4117
- Subject:
- recombinant DNA, etc ; EDTA (chelating agent); aquatic environment; genes; ionic strength; pH; plasmids; pollution; risk; sodium chloride; Show all 10 Subjects
- Abstract:
- ... It has been shown that thermal-treatment at 100°C can denature deoxyribonucleic acid (DNA), yet this does not cause it to break down completely. To clarify the risk of gene pollution from thermal-treated recombinant DNA, the renaturation characteristics of thermal-denatured plasmid pET-28b and its persistence in aquatic environments were investigated. The results revealed that the double-stranded ...
- DOI:
- 10.1080/10934529.2011.623940
- PubMed:
- 22092240
- http://dx.doi.org/10.1080/10934529.2011.623940
- Author:
- Porro, Danilo; Gasser, Brigitte; Fossati, Tiziana; Maurer, Michael; Branduardi, Paola; Sauer, Michael; Mattanovich, Diethard
- Source:
- Applied microbiology and biotechnology 2011 v.89 no.4 pp. 939-948
- ISSN:
- 0175-7598
- Subject:
- recombinant DNA, etc ; biodiversity; eukaryotic cells; hosts; humans; insulin; markets; metabolites; peptides; product development; production technology; recombinant proteins; ribosomal DNA; space and time; yeasts; Show all 15 Subjects
- Abstract:
- ... Recombinant DNA (rDNA) technologies allow the production of a wide range of peptides, proteins and metabolites from naturally non-producing cells. Since human insulin was the first heterologous compound produced in a laboratory in 1977, rDNA technology has become one of the most important technologies developed in the 20th century. Recombinant protein and metabolites production is a multi-billion ...
- DOI:
- 10.1007/s00253-010-3019-z
- http://dx.doi.org/10.1007/s00253-010-3019-z
- Author:
- Grant-Klein, Rebecca J.; Altamura, Louis A.; Schmaljohn, Connie S.
- Source:
- Virus research 2011 v.162 no.1-2 pp. 148-161
- ISSN:
- 0168-1702
- Subject:
- recombinant DNA, etc ; public health; animals; vaccines; Lassa mammarenavirus; infrastructure; biosafety; pathogenicity; vaccine development; viruses; immune response; immune system; genetic variation; markets; Show all 14 Subjects
- Abstract:
- ... Developing vaccines for highly pathogenic viruses such as those causing Lassa, Ebola, and Marburg hemorrhagic fevers is a daunting task due to both scientific and logistical constraints. Scientific hurdles to overcome include poorly defined relationships between pathogenicity and protective immune responses, genetic diversity of viruses, and safety in a target population that includes a large numb ...
- DOI:
- 10.1016/j.virusres.2011.09.005
- http://dx.doi.org/10.1016/j.virusres.2011.09.005
- Author:
- Mallikarjuna, Nalini; Senapathy, Senthilvel; Jadhav, Deepak R.; Saxena, Kulbhushan; Sharma, Hari C.; Upadhyaya, Hari D.; Rathore, Abishek; Varshney, Rajeev
- Source:
- Plant breeding 2011 v.130 no.5 pp. 507-514
- ISSN:
- 0179-9541
- Subject:
- recombinant DNA, etc ; Bruchidae; Cajanus cajan; Phytophthora; backcrossing; blight; color; cytoplasmic male sterility; disease resistance; genes; genetic background; genetic improvement; genetic recombination; growth habit; interspecific hybridization; nucleotide sequences; parents; pest resistance; pigeon peas; plant breeding; pollination; progeny; seeds; tissue culture; wild relatives; Show all 25 Subjects
- Abstract:
- ... With 1 figure and 4 tables ABSTRACT: Cultivated pigeonpea (Cajanus cajan L. Millsp.) has a narrow genetic base; hence, utilization of wild relatives in the crossing programme would broaden its genetic base and introduce useful traits. Cajanus platycarpus (Benth.) Maesen, an annual wild relative from the tertiary gene pool, was successfully crossed with the cultigen, utilizing hormone‐aided pollina ...
- DOI:
- 10.1111/j.1439-0523.2011.01870.x
- http://dx.doi.org/10.1111/j.1439-0523.2011.01870.x
- Author:
- Rothe, Achim; Rubbert, Andrea
- Source:
- New biotechnology 2011 v.28 no.5 pp. 502-510
- ISSN:
- 1871-6784
- Subject:
- recombinant DNA, etc ; anti-inflammatory agents; clinical trials; inflammation; pharmacokinetics; recombinant antibodies; recombinant proteins; technology; therapeutics; Show all 9 Subjects
- Abstract:
- ... New targeted anti-inflammatory drugs have revolutionized the therapeutic strategies in rheumatology. Recombinant DNA selection technologies have enabled the isolation and humanization of specific antibody fragments of any specificity that can be ‘armed’ to deliver effective anti-inflammatory ‘payloads’. Antibodies and other targeted provide the opportunity to block key soluble mediators of inflamm ...
- DOI:
- 10.1016/j.nbt.2011.03.019
- http://dx.doi.org/10.1016/j.nbt.2011.03.019
- Author:
- Yu, Hui-Lin; Li, Yun-He; Wu, Kong-Ming
- Source:
- Journal of integrative plant biology 2011 v.53 no.7 pp. 520-538
- ISSN:
- 1672-9072
- Subject:
- recombinant DNA, etc ; Bacillus thuringiensis; beneficial insects; corn; cotton; crops; ecosystem services; genetic engineering; integrated pest management; management systems; nontarget organisms; pest control; pesticide application; pests; planting; risk assessment; Show all 16 Subjects
- Abstract:
- ... Kong-Ming Wu(Corresponding author) The application of recombinant DNA technology has resulted in many insect-resistant varieties by genetic engineering (GE). Crops expressing Cry toxins derived from Bacillus thuringiensis (Bt) have been planted worldwide, and are an effective tool for pest control. However, one ecological concern regarding the potential effects of insect-resistant GE plants on non ...
- DOI:
- 10.1111/j.1744-7909.2011.01047.x
- PubMed:
- 21564541
- http://dx.doi.org/10.1111/j.1744-7909.2011.01047.x
- Author:
- Amorim, Henrique V.; Lopes, Mário Lucio; de Castro Oliveira, Juliana Velasco; Buckeridge, Marcos S.; Goldman, Gustavo Henrique
- Source:
- Applied microbiology and biotechnology 2011 v.91 no.5 pp. 1267-1275
- ISSN:
- 0175-7598
- Subject:
- recombinant DNA, etc ; economics; sugarcane; feedstocks; carbon dioxide; sugars; energy balance; photosynthesis; cleaning; yeasts; environmental sustainability; ethanol production; biomass; ethanol; alcoholic fermentation; bioethanol; breeding; gasoline; crops; Brazil; Show all 20 Subjects
- Abstract:
- ... Bioethanol (fuel alcohol) has been produced by industrial alcoholic fermentation processes in Brazil since the beginning of the twentieth century. Currently, 432 mills and distilleries crush about 625 million tons of sugarcane per crop, producing about 27 billion liters of ethanol and 38.7 million tons of sugar. The production of bioethanol from sugarcane represents a major large-scale technology ...
- DOI:
- 10.1007/s00253-011-3437-6
- PubMed:
- 21735264
- https://dx.doi.org/10.1007/s00253-011-3437-6
- Author:
- Kumar, G. Senthil; Upadhyay, Sanjeev; Mathew, M.K.; Sarma, Siddhartha P.
- Source:
- Biochimica et biophysica acta 2011 v.1814 no.4 pp. 459-469
- ISSN:
- 1878-1454
- Subject:
- recombinant DNA, etc ; Scorpiones; electrophysiology; hydrogen bonding; isotopes; nuclear magnetic resonance spectroscopy; peptides; potassium channels; sampling; structure-activity relationships; toxins; Show all 11 Subjects
- Abstract:
- ... The three dimensional structure of a 32 residue three disulfide scorpion toxin, BTK-2, from the Indian red scorpion Mesobuthus tamulus has been determined using isotope edited solution NMR methods. Samples for structural and electrophysiological studies were prepared using recombinant DNA methods. Electrophysiological studies show that the peptide is active against hKᵥ1.1 channels. The structure o ...
- DOI:
- 10.1016/j.bbapap.2011.01.006
- http://dx.doi.org/10.1016/j.bbapap.2011.01.006
- Author:
- Belanche, A.; Abecia, L.; Holtrop, G.; Guada, J. A.; Castrillo, C.; de la Fuente, G.; Balcells, J.
- Source:
- Journal of animal science 2011 v.89 no.12 pp. 4163-4174
- ISSN:
- 0021-8812
- Subject:
- recombinant DNA, etc ; Protozoa; abomasum; alfalfa hay; alkanes; ammonia; bacteria; barley; digestibility; ewes; experimental diets; genetic markers; germ-free animals; lambs; microbial proteins; nitrogen metabolism; nucleotide sequences; parturition; protein synthesis; purines; quantitative analysis; rumen; rumen fermentation; rumen protozoa; twins; Show all 25 Subjects
- Abstract:
- ... The aim of this study was to investigate the effect of presence or absence of protozoa on rumen fermentation and efficiency of microbial protein synthesis under different diets. Of 20 twin paired lambs, 1 lamb of each pair was isolated from the ewe within 24 h after birth and reared in a protozoa-free environment (n = 10), whereas their respective twin-siblings remained with the ewe (faunated, n = ...
- DOI:
- 10.2527/jas.2010-3703
- PubMed:
- 21724942
- http://dx.doi.org/10.2527/jas.2010-3703
- Author:
- Alfasi, Sara; Sevastsyanovich, Yanina; Zaffaroni, Lenny; Griffiths, Lesley; Hall, Richard; Cole, Jeffrey
- Source:
- Journal of biotechnology 2011 v.156 no.1 pp. 11-21
- ISSN:
- 0168-1656
- Subject:
- recombinant DNA, etc ; DNA-directed RNA polymerase; Escherichia coli; agar; cytochrome c; cytoplasm; flow cytometry; fluorescence; gene expression; genes; growth retardation; host strains; hosts; mutants; mutation; outer membrane proteins; peroxidase; protein synthesis; recombinant proteins; Show all 19 Subjects
- Abstract:
- ... High level expression of a recombinant gene results in growth arrest, followed by overgrowth by non-productive derivatives. Two methods are described for the isolation of E. coli BL21* strains that are improved hosts for recombinant protein production. Both are based upon the observations (i) that fluorescence of a C-terminal GFP tag is a reliable reporter of the production and correct folding of ...
- DOI:
- 10.1016/j.jbiotec.2011.08.016
- PubMed:
- 21875628
- http://dx.doi.org/10.1016/j.jbiotec.2011.08.016
- Author:
- Selvarajan, R.; Balasubramanian, V.; Sheeba, M.M.; Mohan, R. Raj; Mustaffa, M.M.
- Source:
- Acta horticulturae 2011 no.897 pp. 463-469
- ISSN:
- 0567-7572
- Subject:
- recombinant DNA, etc ; Banana bract mosaic virus; Banana bunchy top virus; Cucumber mosaic virus; Musa; RNA-directed DNA polymerase; antiserum; bananas; biotechnology; enzyme-linked immunosorbent assay; industry; livelihood; pathogens; planting; production technology; reverse transcriptase polymerase chain reaction; tissue culture; virology; viruses; India; Show all 20 Subjects
- Abstract:
- ... Banana and plantain (Musa spp.) are the most important fruit crop in India and play a major role in the livelihood of millions of resource-poor small farmers. Use of quality planting material is very important for increasing productivity. Though conventional suckers are still the primary planting material, use of tissue-culture plants has increased because of their advantages, like more uniform bu ...
- Author:
- Wadhwa, Meenu; Kang, Hye-Na; Knezevic, Ivana; Thorpe, Robin; Griffiths, Elwyn
- Source:
- Biologicals 2011 v.39 no.5 pp. 349-357
- ISSN:
- 1045-1056
- Subject:
- recombinant DNA, etc ; Food and Drug Administration; World Health Organization; case studies; clinical trials; guidelines; monitoring; terminology; South Korea; Show all 9 Subjects
- Abstract:
- ... In August 2010, the World Health Organization and the Korea Food & Drug Administration jointly organized the first implementation workshop of WHO guidelines on evaluating similar biotherapeutic products (SBPs) at the global level. The objective of the Workshop was to facilitate implementation of the newly adopted WHO Guidelines into the practice of national regulatory authorities (NRAs). WHO Guide ...
- DOI:
- 10.1016/j.biologicals.2011.08.009
- http://dx.doi.org/10.1016/j.biologicals.2011.08.009
- Author:
- Kim, Joungmok; Park, Hae-Chul; Gedi, Vinayakumar; Park, Hye-Yeon; Roberts, Arthur G.; Atkins, William M.; Yoon, Moon-Young
- Source:
- Biochemical and biophysical research communications 2011 v.404 no.1 pp. 517-522
- ISSN:
- 0006-291X
- Subject:
- recombinant DNA, etc ; Bacillus anthracis; anthrax; antitoxins; binding sites; enzyme activity; eukaryotic cells; gene expression; genetic transformation; in vitro studies; macrophages; metabolic inhibitors; proteolysis; reporter genes; screening; therapeutics; toxicity; transcription factors; yeasts; Show all 19 Subjects
- Abstract:
- ... Inhibitors of anthrax lethal factor (LF) are currently being sought as effective therapeutics for the treatment of anthrax. Here we report a novel screening approach for inhibitors of LF, a yeast-hybrid-based assay system in which the expression of reporter genes from a Gal4 promoter is repressed by LF proteolytic activity. Yeast cells were co-transformed with LF and a chimeric transcription facto ...
- DOI:
- 10.1016/j.bbrc.2010.12.015
- PubMed:
- 21144836
- http://dx.doi.org/10.1016/j.bbrc.2010.12.015