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You searched for: Subject "recombinant DNA" Remove constraint Subject: "recombinant DNA" Publication Year 2011 Remove constraint Publication Year: 2011
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1. A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus–poxvirus prime boost immunisations

Author:
Ranasinghe, Charani; Eyers, Fiona; Stambas, John; Boyle, David B.; Ramshaw, Ian A.; Ramsay, Alistair J.
Source:
Vaccine 2011 v.29 no.16 pp. 3008-3020
ISSN:
0264-410X
Subject:
recombinant DNA,   etc   ; CD8-positive T-lymphocytes; Human immunodeficiency virus 1; blood serum; chemokines; fowl pox; immunity; immunization; immunoglobulin G; mice; plasmids; ribosomal DNA; spleen; vaccines;     Show all 14 Subjects
Abstract:
... In this study we have firstly compared a range of recombinant DNA poxvirus prime-boost immunisation strategies and shown that combined intramuscular (i.m.) 2× DNA-HIV/intranasal (i.n.) 2× FPV-HIV prime-boost immunisation can generate high-level of HIV-specific systemic (spleen) and mucosal (genito-rectal nodes, vaginal tissues and lung tissues) T cell responses and HIV-1 p24 Gag-specific serum IgG ...
DOI:
10.1016/j.vaccine.2011.01.106
PubMed:
21352941
PubMed Central:
PMC3244379

2. Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation

Author:
Pinyachat, Anuwat; Rojnuckarin, Ponlapat; Muanpasitporn, Chuanchom; Singhamatr, Pon; Nuchprayoon, Surang
Source:
Toxicon 2011 v.57 no.5 pp. 772-780
ISSN:
0041-0101
Subject:
recombinant DNA,   etc   ; Komagataella pastoris; sequence alignment; metalloproteinases; polyacrylamide gel electrophoresis; Western blotting; antivenoms; inhibitory concentration 50; basement membrane; venoms; placenta; signal peptide; patients; pathogenesis; cysteine; complementary DNA; humans; collagen; platelet aggregation; molecular cloning; therapeutics; snakes;     Show all 22 Subjects
Abstract:
... Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using re ...
DOI:
10.1016/j.toxicon.2011.02.011

3. Antimicrobial functionalized genetically engineered spider silk

Author:
Gomes, Sílvia C.; Leonor, Isabel B.; Mano, João F.; Reis, Rui L.; Kaplan, David L.
Source:
Biomaterials 2011 v.32 no.18 pp. 4255-4266
ISSN:
0142-9612
Subject:
recombinant DNA,   etc   ; Araneae; Fourier transform infrared spectroscopy; Staphylococcus aureus; anti-infective properties; biocompatible materials; bioengineering; chimerism; consensus sequence; crosslinking; hepcidin; humans; light scattering; neutrophils; nucleotide sequences; osteosarcoma; recombinant proteins; reflectance; silk; silk proteins;     Show all 20 Subjects
Abstract:
... Genetically engineered fusion proteins offer potential as multifunctional biomaterials for medical use. Fusion or chimeric proteins can be formed using recombinant DNA technology by combining nucleotide sequences encoding different peptides or proteins that are otherwise not found together in nature. In the present study, three new fusion proteins were designed, cloned and expressed and assessed f ...
DOI:
10.1016/j.biomaterials.2011.02.040
PubMed:
21458065
PubMed Central:
PMC3081935

4. Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria—mini review

Author:
Akhverdyan, Valerii Z.; Gak, Evgueni R.; Tokmakova, Irina L.; Stoynova, Nataliya V.; Yomantas, Yurgis A. V.; Mashko, Sergey V.
Source:
Applied microbiology and biotechnology 2011 v.91 no.4 pp. 857-871
ISSN:
0175-7598
Subject:
recombinant DNA,   etc   ; Escherichia coli; Gram-negative bacteria; Methylophilus methylotrophus; bacteriophages; chromosomes; enhancer elements; gene targeting; plasmids; transposition (genetics);     Show all 10 Subjects
Abstract:
... The advantages of phage Mu transposition-based systems for the chromosomal editing of plasmid-less strains are reviewed. The cis and trans requirements for Mu phage-mediated transposition, which include the L/R ends of the Mu DNA, the transposition factors MuA and MuB, and the cis/trans functioning of the E element as an enhancer, are presented. Mini-Mu(LR)/(LER) units are Mu derivatives that lack ...
DOI:
10.1007/s00253-011-3416-y
PubMed:
21698377
PubMed Central:
PMC3145075

5. A quantitative view on Mycobacterium leprae antigens by proteomics

Author:
Wiker, Harald G.; Tomazella, Gisele G.; de Souza, Gustavo A.
Source:
Journal of proteomics 2011 v.74 no.9 pp. 1711-1719
ISSN:
1874-3919
Subject:
recombinant DNA,   etc   ; DNA libraries; Mycobacterium leprae; antigens; bioinformatics; genomics; immune response; leprosy; pathogens; prediction; proteins; proteomics; pseudogenes; virulence;     Show all 14 Subjects
Abstract:
... Leprosy is an ancient disease and the focus of the researchers' scrutiny for more than a century. However, many of the molecular aspects related to transmission, virulence, antigens and immune responses are far from known. Initially, the implementation of recombinant DNA library screens raised interesting antigen candidates. Finally, the availability of Mycobacterium leprae genomic information sho ...
DOI:
10.1016/j.jprot.2011.01.004
PubMed:
21278007

6. Bewertung von real-time PCR-Befunden im Bereich der Nachweisgrenze

Author:
Waiblinger, Hans-Ulrich; Graf, Norbert; Broll, Hermann; Grohmann, Lutz; Pietsch, Klaus
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit = 2011 v.6 no.4 pp. 411-417
ISSN:
1661-5751
Subject:
recombinant DNA,   etc   ; detection limit; genetically modified organisms; quality control; quantitative polymerase chain reaction;     Show all 5 Subjects
Abstract:
... Real-time polymerase chain reaction (real-time PCR) facilitates to detect DNA fragments at very low copy numbers. Positive results, e.g. for unauthorized genetically modified organism (GMO) contamination or for allergens, may raise safety concerns and have far-reaching consequences. However, in case of very low concentrations of DNA samples, results for the same product lot or even for identical s ...
DOI:
10.1007/s00003-011-0669-4

7. Biosimilars – Global issues, national solutions

Author:
Knezevic, Ivana; Griffiths, Elwyn
Source:
Biologicals 2011 v.39 no.5 pp. 252-255
ISSN:
1045-1056
Subject:
recombinant DNA,   etc   ; biological production; biopharmaceutical industry; biopharmaceuticals; information exchange; interferons; markets; patents; patient care; quality of life; somatotropin;     Show all 11 Subjects
Abstract:
... Biotechnology derived medicinal products are presently the best characterized biologicals with considerable production and clinical experience, and have revolutionized the treatment of some of the most difficult-to-treat diseases, prolonging and improving the quality of life and patient care. They are also currently one of the fastest growing segments of the pharmaceutical industry market. The cri ...
DOI:
10.1016/j.biologicals.2011.09.005

8. 2-DE and MALDI-TOF-MS analysis of therapeutic fusion protein abatacept

Author:
Nebija, Dashnor; Urban, Ernst; Stessl, Martina; Noe, Christian R.; Lachmann, Bodo
Source:
Electrophoresis 2011 v.32 no.12 pp. 1438-1443
ISSN:
0173-0835
Subject:
recombinant DNA,   etc   ; matrix-assisted laser desorption-ionization mass spectrometry; polyacrylamide gel electrophoresis; post-translational modification; recombinant proteins;     Show all 5 Subjects
Abstract:
... 2-DE and MALDI-TOF MS are useful techniques for the quality evaluation of medicinal products derived from recombinant DNA technology. The principal objective of this study has been to evaluate the suitability of 2-DE in combination with MALDI-TOF MS for the quality study of the therapeutic recombinant protein, abatacept. 1-DE SDS-PAGE, under reducing and nonreducing conditions, and 2-DE analysis w ...
DOI:
10.1002/elps.201100029

9. Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV

Author:
Moeini, Hassan; Omar, Abdul Rahman; Rahim, Raha Abdul; Yusoff, Khatijah
Source:
Comparative immunology, microbiology, and infectious diseases 2011 v.34 no.3 pp. 227-236
ISSN:
0147-9571
Subject:
recombinant DNA,   etc   ; Chicken anemia virus; anemia; blood serum; chickens; genes; in vivo studies; interferon-gamma; interleukin-2; neutralizing antibodies; plasmids; proteins; recombinant vaccines; splenocytes;     Show all 14 Subjects
Abstract:
... In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were ...
DOI:
10.1016/j.cimid.2010.11.006

10. Enhanced proliferation of neural stem cells in a collagen hydrogel incorporating engineered epidermal growth factor

Author:
Egawa, Edgar Y.; Kato, Koichi; Hiraoka, Makiko; Nakaji-Hirabayashi, Tadashi; Iwata, Hiroo
Source:
Biomaterials 2011 v.32 no.21 pp. 4737-4743
ISSN:
0142-9612
Subject:
recombinant DNA,   etc   ; Parkinson disease; astrocytes; brain; cell proliferation; cell transplantation; collagen; epidermal growth factor; gene expression; hydrocolloids; neurons; polypeptides; rats; stem cells;     Show all 14 Subjects
Abstract:
... Neural stem cells (NSCs) have received much attention in cell-transplantation therapy for central nervous disorders such as Parkinson’s disease. However, poor engraftment of transplanted cells limits the efficacy of the treatments. To overcome this problem, collagen-based hydrogels were designed in this study to provide microenvironments for embedded cells to survive and proliferate. Our approach ...
DOI:
10.1016/j.biomaterials.2011.03.033

11. Evaluation of similar biotherapeutic products (SBPs): Scientific principles and their implementation

Author:
Knezevic, Ivana
Source:
Biologicals 2011 v.39 no.5 pp. 256-261
ISSN:
1045-1056
Subject:
recombinant DNA,   etc   ; World Health Organization; chronic diseases; guidelines; markets; patents; patients; prices; public health; therapeutics;     Show all 10 Subjects
Abstract:
... The availability of biotherapeutic products to patients has a major impact on the success in treating many life-threatening and chronic diseases. These products are often derived by recombinant DNA technology and are expensive for the majority of patients who need them most. Increasing numbers of patents/data protection are now expiring and biologicals “similar” to the originators (innovative prod ...
DOI:
10.1016/j.biologicals.2011.08.008

12. Expression, purification and characterization of cloning-grade zinc finger nuclease

Author:
Tovkach, Andriy; Zeevi, Vardit; Tzfira, Tzvi
Source:
Journal of biotechnology 2011 v.151 no.1 pp. 1-8
ISSN:
0168-1656
Subject:
recombinant DNA,   etc   ; biotechnology; engineering; molecular cloning; nucleotide sequences; purification methods; restriction endonucleases; zinc finger motif;     Show all 8 Subjects
Abstract:
... The limited number of naturally occurring rare-cutting restriction enzymes and the slow and tedious engineering of existing restriction enzymes for novel specificities have prompted the design of new strategies for the development of restriction enzymes with specificities for long DNA sequences. One possibility is using zinc finger nucleases (ZFNs)—synthetic restriction enzymes that are custom-d ...
DOI:
10.1016/j.jbiotec.2010.10.071

13. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate

Author:
Darvishi, Farshad; Destain, Jacqueline; Nahvi, Iraj; Thonart, Philippe; Zarkesh-Esfahani, Hamid
Source:
New biotechnology 2011 v.28 no.6 pp. 756-760
ISSN:
1871-6784
Subject:
recombinant DNA,   etc   ; Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; selective media; sequence analysis; temperature; value-added products; yeasts;     Show all 19 Subjects
Abstract:
... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...
DOI:
10.1016/j.nbt.2011.02.002

14. Identification of a polymeric β-cyclodextrin-binding peptide from a phage-displayed peptide library

Author:
Kang, Boram; Choi, Suk-Jung
Source:
Analytical biochemistry 2011 v.415 no.1 pp. 46-51
ISSN:
0003-2697
Subject:
recombinant DNA,   etc   ; Hepatitis C virus; antibody detection; beta-cyclodextrin; binding capacity; detection limit; dissociation; immunoassays; peptide libraries; proteins;     Show all 10 Subjects
Abstract:
... In this work, a phage-displayed peptide library was applied to identification of β-cyclodextrin (CD)-binding peptide tag, capable of being combined to target peptides or proteins in a homogeneous way by established methods such as peptide synthesis and the recombinant DNA technique. Four enriched sequences were obtained after five rounds of biopanning against polymeric β-CD beads. One of the seque ...
DOI:
10.1016/j.ab.2011.04.016

15. Immunization with recombinant DNA and modified vaccinia virus Ankara (MVA) vectors delivering PSCA and STEAP1 antigens inhibits prostate cancer progression

Author:
Krupa, Magdalena; Canamero, Marta; Gomez, Carmen E.; Najera, Jose L.; Gil, Jesus; Esteban, Mariano
Source:
Vaccine 2011 v.29 no.7 pp. 1504-1513
ISSN:
0264-410X
Subject:
recombinant DNA,   etc   ; T-lymphocytes; Vaccinia virus; adverse effects; antigens; disease control; disease course; histopathology; mice; models; prostatic neoplasms; secondary immunization; vaccination; vaccines;     Show all 14 Subjects
Abstract:
... Despite recent advances in early detection and improvement of conventional therapies, there is an urgent need for development of additional approaches for prevention and/or treatment of prostate cancer, and the use of immunotherapeutic modalities, such as cancer vaccines, is one of the most promising strategies. In this study, we evaluated the prophylactic efficacy of an active immunization protoc ...
DOI:
10.1016/j.vaccine.2010.12.016

16. Large scale expression and purification of recombinant RNA in Escherichia coli

Author:
Ponchon, Luc; Dardel, Frédéric
Source:
Methods 2011 v.54 no.2 pp. 267-273
ISSN:
1046-2023
Subject:
recombinant DNA,   etc   ; Escherichia coli; RNA; nucleotide motifs;     Show all 4 Subjects
Abstract:
... Stable, folded RNA are involved in many key cellular processes and can be used as tools for biological, pharmacological and/or molecular design studies. However, their widespread use has been somewhat limited by their fragile nature and by the difficulties associated with their production on a large scale, which were limited to in vitro methods. This work reviews the novel techniques recently deve ...
DOI:
10.1016/j.ymeth.2011.02.007

17. Lectins: production and practical applications

Author:
Lam, Sze Kwan; Ng, Tzi Bun
Source:
Applied microbiology and biotechnology 2011 v.89 no.1 pp. 45-55
ISSN:
0175-7598
Subject:
recombinant DNA,   etc   ; Human immunodeficiency virus 1; RNA-directed DNA polymerase; animals; chromatography; erythrocytes; insecticidal properties; lectins; legumes; monosaccharides; oligosaccharides;     Show all 11 Subjects
Abstract:
... Lectins are proteins found in a diversity of organisms. They possess the ability to agglutinate erythrocytes with known carbohydrate specificity since they have at least one non-catalytic domain that binds reversibly to specific monosaccharides or oligosaccharides. This articles aims to review the production and practical applications of lectins. Lectins are isolated from their natural sources by ...
DOI:
10.1007/s00253-010-2892-9
PubMed:
20890754
PubMed Central:
PMC3016214

18. New biological approaches for agriculture: a general perspective

Author:
Puigdomenech, P.
Source:
Acta horticulturae 2011 no.916 pp. 25-29
ISSN:
0567-7572
Subject:
recombinant DNA,   etc   ; agronomic traits; cultivars; domestication; marker-assisted selection;     Show all 5 Subjects
Abstract:
... The origin and the progress of agriculture have relied since its beginning in the Neolithic era in the application of the best available knowledge. Plant domestication involved the identification of a limited number of plant and animal species and a narrow range of variation within the species that allowed their cultivation. Starting at the end of 18th century the recognition of how important agro ...

19. N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells

Author:
Misaki, Ryo; Sakai, Yohei; Omasa, Takeshi; Fujiyama, Kazuhito; Seki, Tatsuji
Source:
Journal of bioscience and bioengineering 2011 v.112 no.5 pp. 476-484
ISSN:
1389-1723
Subject:
recombinant DNA,   etc   ; biopharmaceuticals; desorption; glycosylation; high performance liquid chromatography; humans; ionization; matrix-assisted laser desorption-ionization mass spectrometry; mice; monoclonal antibodies; polysaccharides; recombinant proteins; signal peptide; sporamin; sugars; tobacco; vacuoles;     Show all 17 Subjects
Abstract:
... Recombinant DNA technology enables the use of plants as the host for the production of pharmaceutical proteins, such as antibodies. The glycosylation of recombinant proteins plays physiological and biological roles. However, because glycosylation in plants is different from that in human cells, the development of glycoengineering is required. In plant cells, glycan structures are shown to correlat ...
DOI:
10.1016/j.jbiosc.2011.07.002

20. Potential ecological risks of thermal-treated waste recombination DNA discharged into an aquatic environment

Author:
Fu, Xiao H.; Wang, Lei; Li, Meng N.; Zeng, Xiao F.; Le, Yi Q.
Source:
Journal of environmental science and health 2011 v.46 no.14 pp. 1640-1647
ISSN:
1532-4117
Subject:
recombinant DNA,   etc   ; EDTA (chelating agent); aquatic environment; genes; ionic strength; pH; plasmids; pollution; risk; sodium chloride;     Show all 10 Subjects
Abstract:
... It has been shown that thermal-treatment at 100°C can denature deoxyribonucleic acid (DNA), yet this does not cause it to break down completely. To clarify the risk of gene pollution from thermal-treated recombinant DNA, the renaturation characteristics of thermal-denatured plasmid pET-28b and its persistence in aquatic environments were investigated. The results revealed that the double-stranded ...
DOI:
10.1080/10934529.2011.623940
PubMed:
22092240