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- Lin, Shumei, et al. Show all 10 Authors
- Archives of virology 2011 v.156 no.1 pp. 1-7
- DNA; Hepatitis B virus; RNA; cultured cells; enzyme-linked immunosorbent assay; granulosa cells; humans; immunocytochemistry; in situ hybridization; ova; polymerase chain reaction
- ... To investigate the infection and replication of hepatitis B virus (HBV) in primary cultured human granulosa cells. Human granulosa cells were cultured with HBV-positive serum. Media were collected and assayed for HBsAg and HBeAg by ELISA, and HBV DNA by quantitative PCR. HBsAg and HBcAg were detected by immunocytochemistry in cultured cells. HBV DNA and RNA were extracted and amplified by nested P ...