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- Omsland, Anders, et al. Show all 8 Authors
- Applied and environmental microbiology 2011 v.77 no.11 pp. 3720-3725
- Coxiella burnetii; Q fever; agarose; animal tissues; citrates; cysteine; electron microscopy; fetal bovine serum; genetic transformation; genome; hosts; mice; oxygen; pathogens; patients; severe combined immunodeficiency
- ... We recently described acidified citrate cysteine medium (ACCM), which supports host cell-free (axenic) growth of Coxiella burnetii. After 6 days of incubation, greater than 3 logs of growth was achieved with the avirulent Nine Mile phase II (NMII) strain. Here, we describe modified ACCM and culture conditions that support improved growth of C. burnetii and their use in genetic transformation and p ...
- Omsland, Anders, et al. Show all 10 Authors
- mBio 2011 v.2 no.4 pp. e00175
- Coxiella burnetii; Legionella pneumophila; Leishmania amazonensis; Q fever; apoptosis; bacteria; biogenesis; cytosol; genes; human diseases; humans; macrophages; mutants; parasites; parasitism; pathogenesis; pathogens; proteins; secretion; transposons; vacuoles; virulence
- ... Central to Q fever pathogenesis is replication of the causative agent, Coxiella burnetii, within a phagolysosome-like parasitophorous vacuole (PV) in mononuclear phagocytes. C.Â burnetii modulates PV biogenesis and other host cell functions, such as apoptotic signaling, presumably via the activity of proteins delivered to the host cytosol by a Dot/Icm type IVB secretion system (T4BSS). In this stu ...
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