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- Perdomo, Virginia Gabriela, et al. Show all 6 Authors
- Toxicology 2011 v.285 no.1-2 pp. 18-24
- Western blotting; biotransformation; cytotoxicity; doxorubicin; human cell lines; humans; ketoconazole; messenger RNA; quantitative polymerase chain reaction; small interfering RNA; spironolactone; toxicology; transcription (genetics); verapamil; viability; xenobiotics
- ... We evaluated the effect of spironolactone (SL), a well-known inducer of biotransformation and elimination pathways, on the expression and activity of P-glycoprotein (P-gp/ABCB1/MDR1), a major xenobiotic transporter, in HepG2 cells, as well as the potential mediation of pregnane X nuclear receptor (PXR). Cells were exposed to SL (1, 5, 10, 20 or 50μM) for 48h. Expression of P-gp and its mRNA levels ...