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You searched for: Publication year rev 7990-2010 Remove constraint Publication year rev: 7990-2010 Subject citrates Remove constraint Subject: citrates
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1. 1-Methylmalate from Camu-Camu (Myrciaria dubia) Suppressed D-Galactosamine-Induced Liver Injury in Rats

Author:
Toshiyuki AKACHI; Yasuyuki SHIINA; Takumi KAWAGUCHI; Hirokazu KAWAGISHI; Tatsuya MORITA; Kimio SUGIYAMA
Source:
Bioscience, biotechnology, and biochemistry 2010 v.74 no.3 pp. 573-578
ISSN:
1347-6947
Subject:
Averrhoa carambola; Hylocereus; Myrciaria dubia; acerolas; active ingredients; alanine transaminase; aspartate transaminase; biotechnology; citrates; fractionation; freeze drying; fruits; gel chromatography; liver; malates; solvents
Abstract:
... To evaluate the protective effects of fruit juices against D-galactosamine (GalN)-induced liver injury, lyophilized fruit juices (total 12 kinds) were fed to rats for 7 d, and then we evoked liver injury by injecting GalN. The juice of camu-camu (Myrciaria dubia) significantly suppressed GalN-induced liver injury when the magnitude of liver injury was assessed by plasma alanine aminotransferase an ...
DOI:
10.1271/bbb.90775

2. 3D nanoscale imaging of the yeast, Schizosaccharomyces pombe, by full-field transmission X-ray microscopy at 5.4 keV

Author:
Chen, Jie; Yang, Yunhao; Zhang, Xiaobo; Andrews, Joy C.; Pianetta, Piero; Guan, Yong; Liu, Gang; Xiong, Ying; Wu, Ziyu; Tian, Yangchao
Source:
Analytical and bioanalytical chemistry 2010 v.397 no.6 pp. 2117-2121
ISSN:
1618-2642
Subject:
Schizosaccharomyces pombe; X-radiation; absorption; acetates; citrates; image analysis; lead; microscopy; organelles; screening; yeasts
Abstract:
... Three-dimensional (3D) nanoscale structures of the fission yeast, Schizosaccharomyces pombe, can be obtained by full-field transmission hard X-ray microscopy with 30 nm resolution using synchrotron radiation sources. Sample preparation is relatively simple and the samples are portable across various imaging environments, allowing for high-throughput sample screening. The yeast cells were fixed and ...
DOI:
10.1007/s00216-010-3617-8
PubMed:
20349228
PubMed Central:
PMC2896439

3. Acetylation of Metabolic Enzymes Coordinates Carbon Source Utilization and Metabolic Flux

Author:
Wang, Qijun; Zhang, Yakun; Yang, Chen; Xiong, Hui; Lin, Yan; Yao, Jun; Li, Hong; Xie, Lu; Zhao, Wei; Yao, Yufeng; Ning, Zhi-Bin; Zeng, Rong; Xiong, Yue; Guan, Kun-Liang; Zhao, Shimin; Zhao, Guo-Ping
Source:
Science 2010 v.327 no.5968 pp. 1004-1007
ISSN:
0036-8075
Subject:
Salmonella; acetylation; bacteria; carbon; cell growth; citrates; energy; enzymes; gluconeogenesis; glycolysis; glyoxylate cycle; lysine; mammals; prokaryotic cells
Abstract:
... Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. We demonstrated that central metabolism enzymes in Salmonella were acetylated extensively and differentially in response to different carbon sources, concomitantly with changes in cell growth and metabolic flux. The relative activities of key enzymes controlling the direction of gly ...
DOI:
10.1126/science.1179687
PubMed:
20167787
PubMed Central:
PMC4183141

4. Adsorption of copper-citrate complexes on chitosan: Equilibrium modeling

Author:
Lu, Pei-Jen; Hu, Wen-Wei; Chen, Tai-Shang; Chern, Jia-Ming
Source:
Bioresource technology 2010 v.101 no.4 pp. 1127-1134
ISSN:
0960-8524
Subject:
citrates; simulation models; copper; chitosan; pH; amines; adsorption
Abstract:
... The effects of complexes on the adsorption of copper from citrate solutions using chitosan were investigated. Various copper to citrate ratios and solution pHs were used to determine the copper adsorption capacity on the chitosan and a mathematical model was developed to simulate the copper adsorption process. According to the model, the three parameters influencing the copper adsorption capacity ...
DOI:
10.1016/j.biortech.2009.09.055
PubMed:
19822423

5. Aerobic degradation of 2,4,6-trinitrotoluene by the yeast strain Geotrichum candidum AN-Z4

Author:
Ziganshin, A. M.; Gerlach, R.; Naumenko, E. A.; Naumova, R. P.
Source:
Microbiology 2010 v.79 no.2 pp. 178-183
ISSN:
0026-2617
Subject:
Geotrichum candidum; aeration; biodegradation; bioremediation; citrates; hydrides; ions; nitrates; nitrites; nitrogen; soil; succinic acid; trinitrotoluene; yeasts
Abstract:
... The yeast strain Geotrichum candidum AN-Z4 isolated from an anthropogenically polluted site was able to transform 2,4,6-trinitrotoluene (TNT) via the formation of unstable intermediate hydride Meisenheimer complexes with their subsequent destruction and accumulation of nitrite and nitrate ions as the end mineral forms of nitrogen. Aeration of the medium promoted more profound destruction of this x ...
DOI:
10.1134/S0026261710020086

6. Alteration of cell-wall porosity is involved in osmotic stress-induced enhancement of aluminium resistance in common bean (Phaseolus vulgaris L.)

Author:
Yang, Zhong-Bao; Eticha, Dejene; Rao, Idupulapati Madhusudana; Horst, Walter Johannes
Source:
Journal of experimental botany 2010 v.61 no.12 pp. 3245-3258
ISSN:
0022-0957
Subject:
Phaseolus vulgaris; aluminum; apoplast; beans; binding capacity; cations; citrates; crop production; dialysis; drought; exudation; genotype; hydroponics; polyethylene glycol; porosity; root growth; root tips; toxicity; tropics; water stress
Abstract:
... Aluminium (Al) toxicity and drought are the two major abiotic stress factors limiting common bean production in the tropics. Using hydroponics, the short-term effects of combined Al toxicity and drought stress on root growth and Al uptake into the root apex were investigated. In the presence of Al stress, PEG 6000 (polyethylene glycol)-induced osmotic (drought) stress led to the amelioration of Al ...
DOI:
10.1093/jxb/erq146
PubMed:
20511277
PubMed Central:
PMC2905193

7. Aluminum resistance in common bean (Phaseolus vulgaris) involves induction and maintenance of citrate exudation from root apices

Author:
Rangel, Andrés Felipe; Rao, Idupulapati Madhusudana; Braun, Hans-Peter; Horst, Walter Johannes
Source:
Physiologia plantarum 2010 v.138 no.2 pp. 176-190
ISSN:
0031-9317
Subject:
6-phosphofructokinase; NAD (coenzyme); Phaseolus vulgaris; aluminum; beans; citrate (si)-synthase; citrates; exudation; isocitrate dehydrogenase (NADP); malate dehydrogenase; phosphoenolpyruvate carboxylase; root growth; root tips
Abstract:
... Two common bean (Phaseolus vulgaris L.) genotypes differing in aluminum (Al) resistance, Quimbaya (Al-resistant) and VAX-1 (Al-sensitive) were grown in hydroponics for up to 25 h with or without Al, and several parameters related to the exudation of organic acids anions from the root apex were investigated. Al treatment enhanced the exudation of citrate from the root tips of both genotypes. Howeve ...
DOI:
10.1111/j.1399-3054.2009.01303.x
PubMed:
20053183

8. Ancylobacter abiegnus sp. nov., an oligotrophic member of the xylotrophic mycobacterial community

Author:
Zaichikova, M. V.; Berestovskaya, Yu. Yu.; Akimov, V. N.; Kizilova, A. K.; Vasilieva, L. V.
Source:
Microbiology 2010 v.79 no.4 pp. 483-490
ISSN:
0026-2617
Subject:
Ancylobacter; DNA; acetates; bacteria; citrates; ecophysiology; fimbriae; fungi; gluconates; malates; new species; oxalates; pH; phylogeny; pyruvic acid; sodium chloride; succinic acid; temperature; wood; xylan; xylose
Abstract:
... A new species of the genus Ancylobacter, exemplified by strain Z-0056, was isolated from dystrophic humified waters formed by xylotrophic fungi grown on decaying spruce wood. The cells of strain Z-0056 (0.65-0.9 μm) are coccoid, gram-negative, with fimbriae, and nonmotile. The strain is pleomorphic and reproduces by nonuniform division. Strain Z-0056 is an aerobic organoheterotroph and a mesophili ...
DOI:
10.1134/S0026261710040107

9. Atypical citrate-fermenting Lactococcus lactis strains isolated from dromedary's milk

Author:
Drici, H.; Gilbert, C.; Kihal, M.; Atlan, D.
Source:
Journal of applied microbiology 2010 v.108 no.2 pp. 647-657
ISSN:
1364-5072
Subject:
Lactococcus lactis subsp. lactis; Leuconostoc; agar; camel milk; casein; citrates; cultured milk starters; lactic acid bacteria; lactose; metabolism; milk; operon; pH; phenotype; plasmids; polymerase chain reaction; proteinases; proteolysis; ribosomal DNA; temperature
Abstract:
... The aim was to isolate and characterize Lactococcus strains with new properties compared to those of usual Lactococcus dairy starters derived from cow's milk. Algerian dromedary's milk was screened for proteolytic isolates able to grow rapidly on agar milk medium. PCR experiments revealed that 74 proteolytic isolates belonged to the genus Lactococcus and harboured the prtP gene encoding the lactoc ...
DOI:
10.1111/j.1365-2672.2009.04459.x
PubMed:
19663815

10. Bio-reduction of Fe(III) ores using three pure strains of Aeromonas hydrophila, Serratia fonticola and Clostridium celerecrescens and a natural consortium

Author:
García-Balboa, C.; Bedoya, I. Chion; González, F.; Blázquez, M.L.; Muñoz, J.A.; Ballester, A.
Source:
Bioresource technology 2010 v.101 no.20 pp. 7864-7871
ISSN:
0960-8524
Subject:
citrates; acidification; Aeromonas hydrophila; weathering; Clostridium celerecrescens; iron; Serratia fonticola; pH; mixed culture; ferrihydrite
Abstract:
... The present work describes a research approach to the anaerobic bioleaching of Fe(III) ores. Three strains (Serratia fonticola, Aeromonas hydrophila and Clostridium celerecrescens) isolated from an acidic abandoned mine were selected to test their ability to reduce dissimilatory Fe(III). Total iron bio-reduction was achieved after 48h using either the consortium or the Aeromonas cultures. In the l ...
DOI:
10.1016/j.biortech.2010.05.015
PubMed:
20627716

11. Biodegradation of BTE in soil by indigenous microbial populations with and without biogenic substrates

Author:
Chong, Nyuk-Min; Chiou, Jing-Ning
Source:
World journal of microbiology & biotechnology 2010 v.26 no.9 pp. 1587-1594
ISSN:
0959-3993
Subject:
benzene; biodegradation; citrates; ethylbenzene; glucose; plate count; soil; toluene
Abstract:
... Degradation of benzene, toluene, and ethylbenzene (BTE) by microbial populations indigenous to the soil and populations proliferated from the indigenous using biogenic substrates were compared. The reaction system consisted of aerobic microcosms representing an unsaturated soil. Microcosms supplemented with glucose and citrate, when compared to the unsupplemented microcosms, showed increases in ba ...
DOI:
10.1007/s11274-010-0334-6

12. Biodegradation of low molecular weight organic compounds and their contribution to heterotrophic soil respiration in three Japanese forest soils

Author:
Fujii, Kazumichi; Hayakawa, Chie; Van Hees, Patrick A. W.; Funakawa, Shinya; Kosaki, Takashi
Source:
Plant and soil 2010 v.334 no.1-2 pp. 475-489
ISSN:
0032-079X
Subject:
Andisols; Inceptisols; Spodosols; acetates; adsorption; aluminum oxide; biodegradation; citrates; dissolved organic carbon; forest soils; glucose; mineralization; molecular weight; oxalates; soil organic carbon; soil profiles; soil respiration; soil solution
Abstract:
... Low molecular weight (LMW) organic compounds in soil solution could be important substrates for heterotrophic soil respiration. The importance of LMW organic compound mineralization in heterotrophic soil respiration needs to be confirmed for different types of soils. The concentrations of LMW organic compounds in soil solution and mineralization kinetics of ¹⁴C-radiolabelled glucose, acetate, oxal ...
DOI:
10.1007/s11104-010-0398-y

13. Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Author:
Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.
Source:
Science of the total environment 2010 v.409 no.2 pp. 384-395
ISSN:
0048-9697
Subject:
Chlorella vulgaris; X-radiation; absorption; adenosine triphosphate; biomass; biosorption; citrates; fluorescence; leachates; metabolism; pH; spectroscopy; uranium
Abstract:
... Biosorption of uranium(VI) by the green alga Chlorella vulgaris was studied at varying uranium concentrations from 5μM to 1mM, and in the environmentally relevant pH range of 4.4 to 7.0. Living cells bind in a 0.1mM uranium solution at pH 4.4 within 5min 14.3±5.5mgU/g dry biomass and dead cells 28.3±0.6mgU/g dry biomass which corresponds to 45% and 90% of total uranium in solution, respectively. D ...
DOI:
10.1016/j.scitotenv.2010.10.011
PubMed:
21047668

14. Changes of metabolic profiles in urine after oral administration of quercetin in rats

Author:
An, Daizhi; Zhang, Qi; Wu, Shengmin; Wei, Jingyu; Yang, Jijun; Dong, Fangting; Yan, Xianzhong; Guo, Changjiang
Source:
Food and chemical toxicology 2010 v.48 no.6 pp. 1521-1527
ISSN:
0278-6915
Subject:
acetates; alanine; antioxidants; body weight; choline; citrates; creatinine; high performance liquid chromatography; mass spectrometry; metabolites; metabolomics; nuclear magnetic resonance spectroscopy; oral administration; quercetin; rats; succinic acid; taurine; toxicology; trimethylamine; urine
Abstract:
... Quercetin has been studied extensively. However, its actions in vivo are not well understood. We investigated the overall metabolic changes in urine after oral quercetin administration in rats and try to provide useful information on the actions of quercetin in vivo. Rats were orally administered a single dose of quercetin aglycon (40 mg/kg body weight). Urine samples were collected and subjected ...
DOI:
10.1016/j.fct.2010.03.019

15. Characterization of foliar manganese (Mn) in Mn (hyper)accumulators using X‐ray absorption spectroscopy

Author:
Fernando, D.R.; Mizuno, T.; Woodrow, I.E.; Baker, A.J.M.; Collins, R.N.
Source:
new phytologist 2010 v.188 no.4 pp. 1014-1027
ISSN:
0028-646X
Subject:
absorption; carboxylic acids; citrates; hyperaccumulators; leaves; malates; manganese; spectroscopy; woody plants; Australia; Japan; New Caledonia
Abstract:
... • Plant hyperaccumulation of the essential nutrient manganese (Mn) is a rare phenomenon most evident in the Western Pacific region, and differs from hyperaccumulation of other elements. • Mn hyperaccumulators employ a variety of species‐dependent spatial distribution patterns in sequestering excess foliar Mn, including primary sequestration in both nonphotosynthetic and photosynthetic tissues. • T ...
DOI:
10.1111/j.1469-8137.2010.03431.x
PubMed:
20819177

16. Characterization of trophic changes and a functional oxidative pentose phosphate pathway in Synechocystis sp. PCC 6803

Author:
Jansén, Tove; Kurian, Dominic; Raksajit, Wuttinun; York, Steve; Summers, Michael L.; Mäenpää, Pirkko
Source:
Acta physiologiae plantarum 2010 v.32 no.3 pp. 511-518
ISSN:
0137-5881
Subject:
proteins; citrates; proteome; NADP-glucose-6-phosphate dehydrogenase; renewable energy sources; Synechocystis; evolution; energy metabolism; glucose; nitrogen; inorganic pyrophosphatase; carbon; phosphates; mutants; oxidation; pentoses; oxygen
Abstract:
... Cyanobacteria have a tremendous activity to adapt to environmental changes of their growth conditions. In this study, Synechocystis sp. PCC 6803 was used as a model organism to focus on the alternatives of cyanobacterial energy metabolism. Glucose oxidation in Synechocystis sp. PCC6803 was studied by inactivation of slr1843, encoding glucose-6-phosphate dehydrogenase (G6PDH), the first enzyme of t ...
DOI:
10.1007/s11738-009-0428-7

17. Chromatographic Zinc Isotope Separation by Chelating Exchange Resin

Author:
Ding, Xingcheng; Nomura, Masao; Suzuki, Tatsuya; Fujii, Yasuhiko
Source:
Chromatographia 2010 v.71 no.3-4 pp. 195-199
ISSN:
0009-5893
Subject:
EDTA (chelating agent); chromatography; citrates; isotopes; ligands; malates; zinc
Abstract:
... Zinc isotope separations were studied by displacement chromatography using the chelating properties of malate, citrate and lactate exchange resin and EDTA as ligands. After each chromatographic operation, the heavier zinc isotopes were found to preferentially fractionated into the carboxylate complex solution phase. The separation coefficients (ε) for zinc isotope separation had the largest value ...
DOI:
10.1365/s10337-009-1448-6
PubMed:
20208981
PubMed Central:
PMC2827798

18. Chromatographic analysis of serotonin, 5-hydroxyindolacetic acid and homovanillic acid in dried blood spots and platelet poor and rich plasma samples

Author:
Saracino, Maria Addolorata; Gerra, Gilberto; Somaini, Lorenzo; Colombati, Marco; Raggi, Maria Augusta
Source:
Journal of chromatography 2010 v.1217 no.29 pp. 4808-4814
ISSN:
0021-9673
Subject:
anion exchange; blood; chromatography; citrates; detection limit; guard cells; homovanillic acid; humans; methanol; patients; pretreatment; serotonin; solid phase extraction; volunteers
Abstract:
... An isocratic high-performance liquid chromatographic method has been developed for the measurement of serotonin, 5-hydroxyindolacetic and homovanillic acids in dried blood spots and in platelet poor and rich plasma samples. Analyses were carried out on a C18 reversed-phase column using a mobile phase composed of 13% methanol and 87% aqueous citrate buffer, containing octanesulfonic and ethylendiam ...
DOI:
10.1016/j.chroma.2010.05.031

19. Citrate transporters play an important role in regulating aluminum-induced citrate secretion in Glycine max

Author:
You, J. F.; Hou, N. N.; Xu, M. Y.; Zhang, H. M.; Yang, Z. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 766-768
ISSN:
0006-3134
Subject:
Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein synthesis; protein synthesis inhibitors; pyridoxal; roots; secretion; soybeans; transporters
Abstract:
... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
DOI:
10.1007/s10535-010-0138-4

20. Citrate, oxalate, sodium, and magnesium levels in fresh juices of three different types of tomatoes: evaluation in the light of the results of studies on orange and lemon juices

Author:
Erdal Yilmaz; Ertan Batislam; Murat Kacmaz; Imge Erguder
Source:
International journal of food sciences and nutrition 2010 v.61 no.4 pp. 339-345
ISSN:
1465-3478
Subject:
calcium; citrates; fruits; lemons; magnesium; nutrition; oxalates; sodium; tomato juice; tomatoes
Abstract:
... Fruit and vegetable juices containing citrate may be recommended as an alternative in mild to moderate level hypocitraturic calcium stone formers who cannot tolerate pharmacological treatment. Tomato has been proved a citrate-rich vegetable. Tomato juice usage as citrate sources in hypocitraturic recurrent stone formers were evaluated in the light of the results of studies on orange and lemon juic ...
DOI:
10.3109/09637480903405570

21. Cleanup of atrazine-contaminated soils: ecotoxicological study on the efficacy of a bioremediation tool with Pseudomonas sp. ADP

Author:
Chelinho, Sónia; Moreira-Santos, Matilde; Lima, Débora; Silva, Cátia; Viana, Paula; André, Sandra; Lopes, Isabel; Ribeiro, Rui; Fialho, Arsénio M.; Viegas, Cristina A.; Sousa, José P.
Source:
Journal of soils and sediments 2010 v.10 no.3 pp. 568-578
ISSN:
1439-0108
Subject:
Pseudomonas; active ingredients; aquatic organisms; atrazine; bacteria; biodegradation; biomass production; bioremediation; chemical analysis; citrates; earthworms; ecosystems; ecotoxicology; environmental impact; leachates; polluted soils; reproduction; soil analysis; soil ecology; soil toxicity; toxicity; toxicity testing; water pollution
Abstract:
... Purpose To mitigate the environmental effects of atrazine, one of the cleanup strategies available is based on the use of atrazine-degrading bacteria. This work aimed to evaluate the efficacy of a previously developed bioremediation tool for atrazine-contaminated soils (combining bioaugmentation with Pseudomonas sp. ADP, hereafter designated as P. ADP, and biostimulation with citrate) on both soil ...
DOI:
10.1007/s11368-009-0145-2

22. Comparative analysis of membranous proteomics of Shewanella decolorationis S12 grown with azo compound or Fe (III) citrate as sole terminal electron acceptor

Author:
Wang, Bo; Xu, Meiying; Sun, Guoping
Source:
Applied microbiology and biotechnology 2010 v.86 no.5 pp. 1513-1523
ISSN:
0175-7598
Subject:
Shewanella decolorationis; anaerobiosis; azo dyes; citrates; dissolved oxygen; iron; mass spectrometry; membrane proteins; proteomics; two-dimensional gel electrophoresis
Abstract:
... Shewanella decolorationis S12 is capable of carrying out anaerobic respiration using azo dyes and Fe (III) citrate as electron acceptors. In the present study, proteomic techniques including two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry were used to analyze the similarity and the dissimilarity of the membr ...
DOI:
10.1007/s00253-010-2475-9
PubMed:
20309544

23. Comparison of dissolved organic matter from sewage sludge and sludge compost as electron shuttles for enhancing Fe(III) bioreduction

Author:
Huang, De-Yin; Zhuang, Li; Cao, Wei-Dong; Xu, Wei; Zhou, Shun-Gui; Li, Fang-Bai
Source:
Journal of soils and sediments 2010 v.10 no.4 pp. 722-729
ISSN:
1439-0108
Subject:
Shewanella; anaerobic conditions; bacteria; citrates; composting; composts; dissolved organic matter; electron transfer; electrons; ferric chloride; ferric oxide; fractionation; humification; iron; land use; oxidants; physicochemical properties; pollutants; sewage sludge; soil; soil amendments; soil remediation; toxicity; ultraviolet-visible spectroscopy
Abstract:
... Purpose Land utilization of sewage sludge and sludge compost is a common practice in many countries. Soils amended with sewage sludge and sludge compost display different physicochemical properties, especially in terms of dissolved organic matter (DOM) composition that affects the electron-donating capacity (EDC) of DOM in soils. The aim of this paper was to compare the EDC of DOM derived from sew ...
DOI:
10.1007/s11368-009-0161-2

24. Comparison of iron chelates and complexes supplied as foliar sprays and in nutrient solution to correct iron chlorosis of soybean

Author:
Rodríguez-Lucena, Patricia; Hernández-Apaolaza, Lourdes; Lucena, Juan J.
Source:
Zeitschrift für Pflanzenernährung und Bodenkunde 2010 v.173 no.1 pp. 120-126
ISSN:
1436-8730
Subject:
Glycine max; amino acids; chelates; chlorosis; citrates; fertilizers; foliar spraying; glycoproteins; humates; hydroponics; iron; lignosulfonates; nutrient solutions; plant growth; plant response; polyamines; roots; shoots; soybeans; transferrin
Abstract:
... The application of synthetic chelates is the most efficient remedy for correcting iron (Fe) chlorosis. However, chelates are usually expensive and nondegradable products. Recently, new degradable chelates have been proposed for their use as Fe fertilizers. Also, Fe complexes cheaper than synthetic chelates and derived from natural products are also used to correct Fe deficiencies. Fifteen products ...
DOI:
10.1002/jpln.200800256

25. Comparison of serum versus plasma collection in gas chromatography - Mass spectrometry-based metabolomics

Author:
Dettmer, Katja; Almstetter, Martin F.; Appel, Inka J.; Nürnberger, Nadine; Schlamberger, Gregor; Gronwald, Wolfram; Meyer, Heinrich H.D.; Oefner, Peter J.
Source:
Electrophoresis 2010 v.31 no.14 pp. 2365-2373
ISSN:
0173-0835
Subject:
amino acids; antioxidants; aspirin; blood serum; cattle; citrates; computer software; electrophoresis; gas chromatography-mass spectrometry; glucose; metabolites; metabolomics; tumor necrosis factors
Abstract:
... Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco ChromaTOF® software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features wit ...
DOI:
10.1002/elps.200900778

26. Concentrations of bovine lactoferrin and citrate in milk during experimental endotoxin mastitis in early- versus late-lactating dairy cows

Author:
Hyvönen, Paula; Haarahiltunen, Taina; Lehtolainen, Tanja; Heikkinen, Jouni; Isomäki, Ritva; Pyörälä, Satu
Source:
Journal of dairy research 2010 v.77 no.4 pp. 474-480
ISSN:
0022-0299
Subject:
dairy cows; chemical concentration; early lactation; citrates; late lactation; milk quality; lactoferrin; milk; disease resistance; udder quarters; endotoxins; bovine mastitis
Abstract:
... Lactoferrin (Lf) is a molecule naturally present in bovine milk that affects the availability and transport systems of iron. Lf also binds endotoxin (lipopolysaccharide, LPS) of Gram-negative bacteria and modulates the immunological response. In the present study, concentrations of bovine Lf (bLf) and citrate in milk were determined in early (EL) and late (LL) lactating dairy cows, using an experi ...
DOI:
10.1017/S0022029910000579
PubMed:
20800005

27. Correlation between citric acid and nitrate metabolisms during CAM cycle in the atmospheric bromeliad Tillandsia pohliana

Author:
Freschi, Luciano; Rodrigues, Maria Aurineide; Tiné, Marco Aurélio Silva; Mercier, Helenice
Source:
Journal of plant physiology 2010 v.167 no.18 pp. 1577-1583
ISSN:
0176-1617
Subject:
Crassulacean acid metabolism; NAD (coenzyme); Tillandsia; adenosine triphosphate; biogeochemical cycles; biosynthesis; carbon dioxide; carboxylation; citrates; citric acid; decarboxylation; enzyme activity; malates; nitrate reduction; nitrogen metabolism; phosphoenolpyruvate carboxylase; phosphorylation; photostability; solar radiation; starch
Abstract:
... Crassulacean acid metabolism (CAM) confers crucial adaptations for plants living under frequent environmental stresses. A wide metabolic plasticity can be found among CAM species regarding the type of storage carbohydrate, organic acid accumulated at night and decarboxylating system. Consequently, many aspects of the CAM pathway control are still elusive while the impact of this photosynthetic ada ...
DOI:
10.1016/j.jplph.2010.06.002
PubMed:
20594612

28. Cryptococcus randhawai sp. nov., a novel anamorphic basidiomycetous yeast isolated from tree trunk hollow of Ficus religiosa (peepal tree) from New Delhi, India

Author:
Khan, Zia U.; Ahmad, Suhail; Hagen, Ferry; Fell, Jack W.; Kowshik, Tusharantak; Chandy, Rachel; Boekhout, Teun
Source:
Antonie van Leeuwenhoek 2010 v.97 no.3 pp. 253-259
ISSN:
0003-6072
Subject:
Cryptococcus albidus; Ficus religiosa; Filobasidiales; citrates; decayed wood; genes; internal transcribed spacers; lactose; myo-inositol; new species; raffinose; ribosomal DNA; ribosomal RNA; sequence analysis; temperature; transcription (genetics); tree trunk; trees; vitamins; yeasts; India
Abstract:
... A novel anamorphic Cryptococcus species is described, which was isolated in New Delhi (India) from decaying wood of a tree trunk hollow of Ficus religiosa. On the basis of sequence analysis of the D1/D2 domains of the 26S rRNA gene and the internally transcribed spacer (ITS)-1 and ITS-2 region sequences, the isolate belonged to the Cryptococcus albidus cluster (Filobasidiales, Tremellomycetes) and ...
DOI:
10.1007/s10482-009-9406-8
PubMed:
20091225

29. Development of a reliable and easy method for screening Oenococcus carbohydrate consumption profile

Author:
B. Hocine; Zohra Jamal; Laurent Riquier; Aline Lonvaud-Funel; Marguerite Dols-Lafargue
Source:
OENO One 2010 v.44 no.1 pp. 31-37
ISSN:
2494-1271
Subject:
Oenococcus oeni; acidification; arabinose; bacterial growth; citrates; colorimetry; culture media; fructose; glucose; inoculum; malic acid; malolactic fermentation; rhamnose; trehalose; wines; xylose
Abstract:
... Aims: The aim of this study was to develop a colorimetric test to determine Oenococcus carbohydrate consumption profile. Methods and results: A semi-defined growth medium which enabled efficient bacterial growth and medium acidification in the presence of glucose was developed. The inoculum size and the presence of citrate in the medium were optimized. Acidification of the medium was revealed by a ...
DOI:
10.20870/oeno-one.2010.44.1.1455

30. Difference in the distribution and speciation of cellular nickel between nickel-tolerant and non-tolerant Nicotiana tabacum L. cv. BY-2 cells

Author:
SAITO, AKIHIRO; SAITO, MISA; ICHIKAWA, YUSUKE; YOSHIBA, MASAAKI; TADANO, TOSHIAKI; MIWA, EITARO; HIGUCHI, KYOKO
Source:
Plant, cell and environment 2010 v.33 no.2 pp. 174-187
ISSN:
0140-7791
Subject:
Nicotiana tabacum; apoplast; cell walls; chelation; citrates; metal ions; nickel; protoplasts; symplast; tobacco; toxicity; vacuoles; viability
Abstract:
... To evaluate Ni dynamics at the subcellular level, the distribution and speciation of Ni were determined in wild-type (WT) and Ni-tolerant (NIT) tobacco BY-2 cell lines. When exposed to low but toxic levels of Ni, NIT cells were found to contain 2.5-fold more Ni (14% of whole-cell Ni values) in their cell walls than WT cells (6% of whole-cell Ni values). In addition to higher levels of Ni in the ap ...
DOI:
10.1111/j.1365-3040.2009.02068.x
PubMed:
19906154

31. Different combinations of salts affect the growth and bacteriocin production by Lactobacillus salivarius CRL 1328

Author:
Juárez Tomás, María Silvina; Bru, Elena; Nader-Macías, María Elena
Source:
Journal of chemical technology and biotechnology 2010 v.85 no.1 pp. 91-99
ISSN:
0268-2575
Subject:
Lactobacillus salivarius; bacteria; bacteriocins; biomass; citrates; culture media; experimental design; magnesium sulfate; probiotics; sodium acetate
Abstract:
... BACKGROUND: The culture medium for optimal growth of vaginal Lactobacillus salivarius CRL 1328 is different from that for optimal bacteriocin production. To simultaneously obtain high amount of biomass and bacteriocin of this microorganism, the effects of different basal culture media and salts on both responses were evaluated. The study was performed by using a complete factorial experimental des ...
DOI:
10.1002/jctb.2271

32. Drought effect on photosynthetic activity, osmolyte accumulation and membrane integrity of two Cicer arietinum genotypes

Author:
Matos, M. C.; Campos, P. S.; Passarinho, J. A.; Semedo, J. N.; Marques, N. M.; Ramalho, J. C.; Ricardo, C. P.
Source:
Photosynthetica 2010 v.48 no.2 pp. 303-312
ISSN:
0300-3604
Subject:
Cicer arietinum; asparagine; biomass; carotenoids; chickpeas; chlorophyll; citrates; drought; drought tolerance; electrolytes; electron transfer; fatty acid composition; fluorescence; genotype; malates; metabolites; photosystem II; physiological response; pinitol; pods; proline; rehydration; roots; shoots; solutes; stomatal conductance; sucrose; water stress
Abstract:
... Drought was induced in chickpea (Cicer arietinum L.) genotypes (ChK 3226 and ILC 3279) differing in yield capacity. Water stress (S1, RWC around 55-50%; S2, RWC ≤ 40%) drastically reduced stomatal conductance (g s) and net photosynthetic rate (P N) in both genotypes. ILC 3279 showed greater photosynthetic capacity (A max) decreases. Maximum PSII photochemical efficiency (Fv/Fm), photochemical quen ...
DOI:
10.1007/s11099-010-0038-z

33. Effect of Citral on the Thermal Inactivation of Escherichia coli O157:H7 in Citrate Phosphate Buffer and Apple Juice

Author:
L. Espina; M. Somolinos; R. Pagan; D. Garcia-Gonzalo
Source:
Journal of food protection 2010 v.73 no.12 pp. 2189-2196
ISSN:
0362-028X
Subject:
Escherichia coli O157; apple juice; citral; citrates; heat; heat inactivation; heat tolerance; heat treatment; pH; phosphates; protective effect
Abstract:
... Inactivation and sublethal injury of Escherichia coli O157:H7 cells induced by heat in citrate phosphate buffer and apple juice (both at pH 3.8) were studied, and the effect of a combined preservation treatment using citral and heat treatments was determined. Heat resistance of E. coli O157:H7 was similar in both treatment media; after 27 min at 54°C, 3 log units of the initial cell population was ...
DOI:
10.4315/0362-028X-73.12.2189

34. Effect of Disaccharides on Ion Properties in Milk-Based Systems

Author:
Gao, Ran; Leeuwen, Herman P. van; Temminghoff, Erwin J.; Valenberg, Hein J. van; Eisner, Matthias D.; Boekel, Martinus A. van
Source:
Journal of agricultural and food chemistry 2010 v.58 no.10 pp. 6449–6457
ISSN:
0021-8561
Subject:
calcium chloride; citrates; thermal properties; milk proteins; calcium; sucrose; cations; milk; pH; electrostatic interactions; potassium chloride; ionic strength
Abstract:
... The mean spherical approximation (MSA) theory is used to explain the impact of sugars on ion properties in milk-based systems by taking into account electrostatic interactions and volume exclusion effects. This study first focuses on the changes in Ca2+ activity and pH in a solution consisting of CaCl2, KCl, and K3citrate, as a function of sucrose concentration. MSA model calculations were compare ...
DOI:
10.1021/jf1002396
PubMed:
20426420

35. Effect of Home-Refrigerator Storage Temperature on Tomato Quality

Author:
Farneti, B.; Zhang, W.; Witkowska, I.; Woltering, E.J.
Source:
Acta horticulturae 2010 v. no.877 pp. 1191-1196
ISSN:
0567-7572
Subject:
carbohydrate content; chilling injury; citrates; cultivars; firmness; freezing point; fruit quality; ripening; shelf life; storage temperature; sugars; titratable acidity; tomatoes; tropical and subtropical fruits; weight loss
Abstract:
... Postharvest storage, handling and distribution of fruit at low temperatures is the most common and manageable approach to control ripening and subsequent deterioration and to maximize product shelf-life. However, tomatoes, as many other subtropical fruits, are susceptible to develop symptoms of chilling injury, a physiological disorder caused by the exposure to low temperature above the freezing p ...
DOI:
10.17660/ActaHortic.2010.877.162

36. Effect of calcium carbonate, calcium citrate, tricalcium phosphate, calcium gluconate and calcium lactate on some physicochemical properties of soymilk

Author:
Pathomrungsiyounggul, Pattavara; Grandison, Alistair S.; Lewis, Michael J.
Source:
International journal of food science & technology 2010 v.45 no.11 pp. 2234-2240
ISSN:
0950-5423
Subject:
calcium; calcium carbonate; citrates; freezing point; lactates; pH; particle size; sediments; solubility; soymilk; tricalcium phosphate; viscosity
Abstract:
... Soymilk fortified with 25 mm Ca (Ca carbonate, Ca citrate, triCa phosphate, Ca gluconate or Ca lactate) was compared with the properties of unfortified soymilk (control). Calcium carbonate, Ca citrate and triCa phosphate did not significantly affect [Ca²⁺], absolute viscosity and particle size of soymilk, but Ca gluconate and Ca lactate significantly increased these properties. The pH of soymilk w ...
DOI:
10.1111/j.1365-2621.2010.02399.x

37. Effect of hypoxia and post-hypoxia on the fluctuations in contents of malate and citrate, the activity of malic enzyme, and on the intensity of gas exchange in moss gametophores

Author:
Rut, G.; Rzepka, A.; Krupa, J.
Source:
Photosynthetica 2010 v.48 no.1 pp. 79-86
ISSN:
0300-3604
Subject:
NADP (coenzyme); Polytrichum; air; calcium chloride; carbon dioxide; citrates; gametophores; gas exchange; habitats; hypoxia; malates; malic enzyme; mosses and liverworts; oxygen; photosynthesis; submerged aquatic plants; submergence; water shortages
Abstract:
... Mosses are plants of simple anatomical structure and as they occur in habitats characterised not only by major changes in the concentrations of carbon dioxide, they suffer the stress of periodic water shortages or submergence in water. The condition of hypoxia (submergence in water or CaCl₂ solution) prompted the increase in daily fluctuations in malate content, particularly in the gametophores of ...
DOI:
10.1007/s11099-010-0011-x

38. Effect of site-directed mutagenesis of citB on the expression and activity of Bacillus subtilis aconitase

Author:
Gao, Weihua; Dai, Sen; Liu, Quanli; Xu, Haijin; Bai, Yanlin; Qiao, Mingqiang
Source:
Microbiology 2010 v.79 no.6 pp. 767-771
ISSN:
0026-2617
Subject:
Bacillus subtilis; RNA; Western blotting; aconitate hydratase; amino acids; beta-galactosidase; citrates; enzyme activity; genes; mutants; protein synthesis; regulatory proteins; site-directed mutagenesis
Abstract:
... Bacillus subtilis aconitase, encoded by the citB gene, is a bifunctional enzyme, which can not only interconvert citrate and isocitrate, but also has the RNA binding function similar to the eukaryotic protein IRP-1 (iron regulatory protein 1). Homology analysis between eukaryotic aconitase and B. subtilis aconitase indicates that the amino acids 741-745 probably have important function for the B. ...
DOI:
10.1134/S002626171006007X

39. Effects of andrographolide on sexual functions, vascular reactivity and serum testosterone level in rodents

Author:
Sattayasai, Jintana; Srisuwan, Supawadee; Arkaravichien, Tarinee; Aromdee, Chantana
Source:
Food and chemical toxicology 2010 v.48 no.7 pp. 1934-1938
ISSN:
0278-6915
Subject:
Andrographis paniculata; blood serum; citrates; dimethyl sulfoxide; laboratory animals; mating behavior; mice; norepinephrine; oral administration; rats; spermatozoa; testosterone; toxicology
Abstract:
... In this study, effects of andrographolide from Andrographis paniculata on sexual functions, vascular reactivity and serum testosterone level in experimental animals were observed. The suspension of andrographolide in 5% DMSO was administered orally at the dose of 50 mg/kg to male ICR mice. The female mice involved in mating were made receptive by hormonal treatment. The mating behaviors, mounting ...
DOI:
10.1016/j.fct.2010.04.037

40. Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine-modified screen-printed carbon electrode and its application in pharmaceutical and food analysis

Author:
Honeychurch, Kevin C.; Gilbert, Lucy; Hart, John P.
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.8 pp. 3103-3111
ISSN:
1618-2642
Subject:
lead; carbon; food analysis; glucose; iron; EDTA (chelating agent); electrodes; hesperidin; oxidation; citric acid; metal ions; cysteine; citrates; sampling; cobalt; copper; calcium; oxalic acid; lime juice; silver
Abstract:
... Cobalt phthalocyanine-modified screen-printed carbon electrodes (CoPC-SPCEs) have been investigated as disposable sensors for the measurement of citric acid. The analyte was found to undergo an electrocatalytic oxidation process involving the Co²⁺/Co³⁺ redox couple. Calibration plots were found to be linear in the range 2 mM to 2.0 M; replicate determinations of a 5.2 mM citric acid (n = 4) soluti ...
DOI:
10.1007/s00216-010-3534-x

41. Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

Author:
Xue, Xuedong; Li, Wei; Li, Zhimin; Xia, Yuelan; Ye, Qin
Source:
Journal of industrial microbiology & biotechnology 2010 v.37 no.7 pp. 681-687
ISSN:
1367-5435
Subject:
Klebsiella pneumoniae; acetates; byproducts; cell growth; citrates; ethanol; lactic acid; succinic acid
Abstract:
... In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-pro ...
DOI:
10.1007/s10295-010-0711-z
PubMed:
20361229

42. Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture

Author:
Omasa, Takeshi; Furuichi, Keisuke; Iemura, Tomoya; Katakura, Yoshio; Kishimoto, Michimasa; Suga, Ken-ichi
Source:
Bioprocess and biosystems engineering 2010 v.33 no.1 pp. 117-125
ISSN:
1615-7591
Subject:
adenosine triphosphate; antibodies; antibody formation; biochemical pathways; citrates; glucose; glutamine; hybridomas; malates; mammals; primary energy; pyruvic acid; specific growth rate
Abstract:
... Generally, mammalian cells utilize glucose and glutamine as primary energy sources. To investigate the effect of energy sources on metabolic fluxes and antibody production, glucose- or glutamine-limited serum-free continuous culture of hybridoma 3A21 cells, which produce anti-ribonuclease A antibody, was carried out. The cell volume and dry cell weight were evaluated under various steady-state con ...
DOI:
10.1007/s00449-009-0351-8

43. Enhancing itaconic acid production by Aspergillus terreus

Author:
Tevž, Gregor; Benčina, Mojca; Legiša, Matic
Source:
Applied microbiology and biotechnology 2010 v.87 no.5 pp. 1657-1664
ISSN:
0175-7598
Subject:
Aspergillus niger; Aspergillus terreus; citrates; excretion; genes; glycolysis; mycelium; pH; phylogeny; post-translational modification; tricarboxylic acid cycle
Abstract:
... Aspergillus terreus is successfully used for industrial production of itaconic acid. The acid is formed from cis-aconitate, an intermediate of the tricarboxylic (TCA) cycle, by catalytic action of cis-aconitate decarboxylase. It could be assumed that strong anaplerotic reactions that replenish the pool of the TCA cycle intermediates would enhance the synthesis and excretion rate of itaconic acid. ...
DOI:
10.1007/s00253-010-2642-z
PubMed:
20461508

44. Evaluation of adverse effects in tamoxifen exposed healthy female dogs

Author:
Wanessa LF Tavares; Gleidice E Lavalle; Mariana S Figueiredo; Aline G Souza; Angelica C Bertagnolli; Fernando AB Viana; Paulo RO Paes; Rubens A Carneiro; Guilherme AO Cavalcanti; Marilia M Melo; Geovanni D Cassali
Source:
Acta veterinaria scandinavica 2010 v.52 no.1 pp. 635
ISSN:
1751-0147
Subject:
adverse effects; body weight; bone marrow; citrates; clinical examination; dogs; drug therapy; edema; estrogens; females; hematology; mammary glands; mammary neoplasms (animal); ophthalmology; pyometra; risk; spaying; tamoxifen; uterus; vulva
Abstract:
... BACKGROUND: Mammary tumors are among the most frequent neoplasms in female dogs, but the strategies employed in animal treatment are limited. In human medicine, hormone manipulation is used in cancer therapy. Tamoxifen citrate is a selective inhibitor of oestrogen receptors and exerts a potent anti-oestrogen effect on the mammary gland. The aim of this study was to evaluate the adverse effects whe ...
DOI:
10.1186/1751-0147-52-67
PubMed:
21176231
PubMed Central:
PMC3022551

45. Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism

Author:
Bohn, Chantal; Rigoulay, Candice; Chabelskaya, Svetlana; Sharma, Cynthia M.; Marchais, Antonin; Skorski, Patricia; Borezée-Durant, Elise; Barbet, Romain; Jacquet, Eric; Jacq, Annick; Gautheret, Daniel; Felden, Brice; Vogel, Jörg; Bouloc, Philippe
Source:
Nucleic acids research 2010 v.38 no.19 pp. 6620-6636
ISSN:
0305-1048
Subject:
ABC transporters; Staphylococcus aureus; carbon; citrates; enzymes; intergenic DNA; messenger RNA; metabolism; operon; pathogens; transcription (genetics)
Abstract:
... Using an experimental approach, we investigated the RNome of the pathogen Staphylococcus aureus to identify 30 small RNAs (sRNAs) including 14 that are newly confirmed. Among the latter, 10 are encoded in intergenic regions, three are generated by premature transcription termination associated with riboswitch activities, and one is expressed from the complementary strand of a transposase gene. The ...
DOI:
10.1093/nar/gkq462
PubMed:
20511587
PubMed Central:
PMC2965222

46. Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

Author:
Porto, T. S.; Marques, P. P.; Porto, C. S.; Moreira, K. A.; Lima-Filho, J. L.; Converti, A.; Pessoa, A.; Porto, A. L. F.
Source:
Applied biochemistry and biotechnology 2010 v.160 no.4 pp. 1057-1064
ISSN:
0273-2289
Subject:
citrates; Cucurbita maxima; ascorbate oxidase; equipment design; polyethylene glycol; extraction; partition coefficients
Abstract:
... The ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. The techniques currently used for the purification of this enzyme raise its production cost. Thus, the development of alternative processes and with the potential to reduce costs is interesting. The application of aqueous two-phase system is proposed as ...
DOI:
10.1007/s12010-009-8631-y
PubMed:
19412579

47. Factors contributing to plasma TCO2 and acid‐base state in Ontario Standardbred racehorses

Author:
A. P. WALLER; W. PEARSON; M. I. LINDINGER
Source:
Equine veterinary journal 2010 v.42 Suppl s38 no. pp. 592-600
ISSN:
0425-1644
Subject:
Standardbred; Thoroughbred; acetates; acid-base balance; alkalosis; bicarbonates; blood; blood proteins; butyrates; carbon dioxide; citrates; data collection; nutrition; racehorses; Ontario
Abstract:
... Reasons for performing study: Standardbred and Thoroughbred racehorses around the world are tested for performance enhancing substances. Among these are blood alkalising substances that raise plasma pH and total carbon dioxide (TCO₂) concentration. However, many horses have an elevated TCO₂ due to dietary, environmental and health concerns without having been administered an alkalising substance. ...
DOI:
10.1111/j.2042-3306.2010.00249.x

48. Fe(III)-enhanced anaerobic transformation of 2,4-dichlorophenoxyacetic acid by an iron-reducing bacterium Comamonas koreensis CY01

Author:
Wu, Chun-Yuan; Zhuang, Li; Zhou, Shun-Gui; Li, Fang-Bai; Li, Xiao-Min
Source:
FEMS microbiology ecology 2010 v.71 no.1 pp. 106-113
ISSN:
0168-6496
Subject:
hematite; citrates; anaerobic conditions; glucose; sucrose; goethite; iron; glycerol; lepidocrocite; ferrihydrite; biodegradation; Comamonas; oxidation; dechlorination; 2,4-D; bacteria
Abstract:
... This work studied the ability of Comamonas koreensis CY01 to reduce Fe(III) (hydr)oxides by coupling the oxidation of electron donors and the enhanced biodegradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the presence of Fe(III) (hydr)oxides. The experimental results suggested that strain CY01 can utilize ferrihydrite, goethite, lepidocrocite or hematite as the terminal electron acceptor and ...
DOI:
10.1111/j.1574-6941.2009.00796.x
PubMed:
19895639

49. Flow field-flow fractionation: a versatile approach for size characterization of α-tocopherol-induced enlargement of gold nanoparticles

Author:
Sermsri, Wimut; Jarujamrus, Purim; Shiowatana, Juwadee; Siripinyanond, Atitaya
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.8 pp. 3079-3085
ISSN:
1618-2642
Subject:
alpha-tocopherol; catalytic activity; citrates; fractionation; monitoring; nanogold; nanoparticles; particle size; transmission electron microscopy
Abstract:
... Flow field-flow fractionation (FlFFF) was used for size characterization of gold nanoparticles. The measured particle sizes obtained from FlFFF for the commercial 10 nm gold nanoparticle standard and the gold nanoparticles synthesized in the laboratory were in good agreement with those measured by transmission electron microscopy (TEM). Further, the capability of α-tocopherol to induce enlargement ...
DOI:
10.1007/s00216-010-3511-4

50. Gene expression analysis in cadmium-stressed roots of a low cadmium-accumulating solanaceous plant, Solanum torvum

Author:
Yamaguchi, Hirotaka; Fukuoka, Hiroyuki; Arao, Tomohito; Ohyama, Akio; Nunome, Tsukasa; Miyatake, Koji; Negoro, Satomi
Source:
Journal of experimental botany 2010 v.61 no.2 pp. 423-437
ISSN:
0022-0957
Subject:
Solanum torvum; acclimation; cadmium; citrates; complementary DNA; eggplants; enzymes; gene expression; gene expression regulation; genes; heavy metals; oxidative stress; reverse transcriptase polymerase chain reaction; roots; sequence analysis; tomatoes; transcription factors; transporters
Abstract:
... Solanum torvum Sw. cv. Torubamubiga (TB) is a low cadmium (Cd)-accumulating plant. To elucidate the molecular mechanisms of the Cd acclimation process in TB roots, transcriptional regulation was analysed in response to mild Cd treatment: 0.1 μM CdCl₂ in hydroponic solution. A unigene set consisting of 6296 unigene sequences was constructed from 18 816 TB cDNAs. The distribution of functional categ ...
DOI:
10.1093/jxb/erp313
PubMed:
19837731
PubMed Central:
PMC2803209

51. Genetic screening of wine-related enzymes in Lactobacillus species isolated from South African wines

Author:
Mtshali, P.S.; Divol, B.; van Rensburg, P.; du Toit, M.
Source:
Journal of applied microbiology 2010 v.108 no.4 pp. 1389-1397
ISSN:
1364-5072
Subject:
DNA primers; Lactobacillus brevis; Lactobacillus hilgardii; Lactobacillus paracasei; Lactobacillus paraplantarum; Lactobacillus pentosus; Lactobacillus plantarum; Lactobacillus sakei; beta-glucosidase; citrates; esterases; genes; genetic databases; genetic variation; harvest date; lactic acid bacteria; malolactic fermentation; odors; polymerase chain reaction; proteinases; screening; sequence analysis; starter cultures; wine quality; wines
Abstract:
... The objective of this study was to investigate the presence of genes coding for enzymes of oenological relevance in wine Lactobacillus strains isolated from South African grape and wine samples during the 2001 and 2002 harvest seasons. A total of 120 wine lactobacilli isolates belonging to Lactobacillus plantarum, Lactobacillus hilgardii, Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus ...
DOI:
10.1111/j.1365-2672.2009.04535.x
PubMed:
19793136

52. High methoxyl pectin/apple particles composite gels: effect of particle size and particle concentration on mechanical properties and gel structure

Author:
GENOVESE, DIEGO B.; YE, AIQAIN; SINGH, HARJINDER
Source:
Journal of texture studies 2010 v.41 no.2 pp. 171-189
ISSN:
0022-4901
Subject:
adhesion; apples; citrates; fruit pulp; gel strength; gels; glucose; jams; mechanical properties; modulus of elasticity; pH; particle size; pectins
Abstract:
... In order to study the effect of fruit pulp on the structure of fruit jams, they were modeled as composites with a high-methoxyl pectin (HMP) gel matrix filled with apple particles. The effect of particle concentration (0, 1 and 2 wt%) and particle size (small: <125 μm, and large: 125-850 μm) on a pectin gel (0.5 wt% HMP, 65 wt% glucose, pH 3.0, 0.1 M citrate buffer) were studied. Rheological measu ...
DOI:
10.1111/j.1745-4603.2010.00220.x

53. Highly selective light scattering imaging of chromium (III) in living cells with silver nanoparticles

Author:
Tong Wu; Chun Liu; Ke Jun Tan; Ping Ping Hu; Cheng Zhi Huang
Source:
Analytical and bioanalytical chemistry 2010 v.397 no.3 pp. 1273-1279
ISSN:
1618-2642
Subject:
aluminum; ambient temperature; bone marrow; calcium; chelation; chromium; citrates; cobalt; color; copper; crosslinking; cytoplasm; humans; image analysis; iron; lanthanum; lead; light scattering; magnesium; mercury; metal ions; nanosilver; nickel; pH; surface plasmon resonance; zinc
Abstract:
... In this contribution, we present a highly selective chromium ion (Cr³⁺)-induced aggregation of citrate-capped silver nanoparticles, which could be applied for the imaging of the distribution of Cr³⁺ in cells. It was found that selective aggregation of citrate-capped silver nanoparticles occurs at room temperature in the presence of Cr³⁺ in aqueous medium of pH 6.8, resulting in color change from y ...
DOI:
10.1007/s00216-010-3619-6

54. Histidine is a source of the antioxidant, α-ketoglutarate, in Pseudomonas fluorescens challenged by oxidative stress

Author:
Lemire, Joseph; Milandu, Yves; Auger, Christopher; Bignucolo, Adam; Appanna, Varun P.; Appanna, Vasu D.
Source:
FEMS microbiology letters 2010 v.309 no.2 pp. 170-177
ISSN:
0378-1097
Subject:
neutralization; carbon dioxide; NAD (coenzyme); alpha-ketoglutaric acid; Pseudomonas fluorescens; citrates; oxidative stress; histidine; succinic acid; hydrogen peroxide; oxidative phosphorylation; succinate dehydrogenase (quinone); homeostasis; tricarboxylic acid cycle; glutamic acid
Abstract:
... The role of α-ketoglutarate (KG) in the detoxification of reactive oxygen species (ROS) has only recently begun to be appreciated. This ketoacid neutralizes ROS in an NADPH-independent manner with the concomitant formation of succinate and CO₂. To further probe this intriguing attribute of KG in living systems, we have evaluated the significance of histidine metabolism in the model organism, Pseud ...
DOI:
10.1111/j.1574-6968.2010.02034.x

55. How Pseudomonas aeruginosa adapts to various environments: a metabolomic approach

Author:
Frimmersdorf, Eliane; Horatzek, Sonja; Pelnikevich, Anya; Wiehlmann, Lutz; Schomburg, Dietmar
Source:
Environmental microbiology 2010 v.12 no.6 pp. 1734-1747
ISSN:
1462-2912
Subject:
citrates; proteome; energy; glucose; genetic background; glutamates; cadaverine; biochemical pathways; carbon; metabolome; transcriptome; Pseudomonas aeruginosa; amino acid metabolism; metabolomics
Abstract:
... In addition to transcriptome and proteome studies, metabolome analysis represents a third complementary approach to identify metabolic pathways and adaptation processes. In order to elucidate basic principles of metabolic versatility of Pseudomonas aeruginosa, we investigated the metabolome profiles of two genetically and morphologically divergent strains, the reference strain PAO1 and the mucoid ...
DOI:
10.1111/j.1462-2920.2010.02253.x
PubMed:
20553553

56. How a microbial drug transporter became essential for crop cultivation on acid soils: aluminium tolerance conferred by the multidrug and toxic compound extrusion (MATE) family

Author:
Magalhaes, Jurandir V.
Source:
Annals of botany 2010 v.106 no.1 pp. 199-203
ISSN:
0305-7364
Subject:
aluminum; arable soils; barley; citrates; crops; drugs; extrusion; functional diversity; genes; loci; protein transport; substrate specificity; tillage; toxicity; transporters
Abstract:
... BACKGROUND: Aluminium (Al) toxicity is a major agricultural constraint for crop cultivation on acid soils, which comprise a large portion of the world's arable land. One of the most widely accepted mechanisms of Al tolerance in plants is based on Al-activated organic acid release into the rhizosphere, with organic acids forming stable, non-toxic complexes with Al. This mechanism has recently been ...
DOI:
10.1093/aob/mcq115
PubMed:
20511585
PubMed Central:
PMC2889808

57. Identification and Characterization of a Novel CprA Reductive Dehalogenase Specific to Highly Chlorinated Phenols from Desulfitobacterium hafniense Strain PCP-1

Author:
Bisaillon, Ariane; Beaudet, Réjean; Lépine, François; Déziel, Eric; Villemur, Richard
Source:
Applied and environmental microbiology 2010 v.76 no.22 pp. 7536-7540
ISSN:
0099-2240
Subject:
Desulfitobacterium hafniense; citrates; dechlorination; gel electrophoresis; genome; half life; mass spectrometry; molecular weight; oxygen; pH; pentachlorophenol; phenols; sodium; temperature; titanium
Abstract:
... Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purified a novel RDase involved in the dechlorination of highly chlorinated phenols fr ...
DOI:
10.1128/AEM.01362-10
PubMed:
20870790
PubMed Central:
PMC2976209

58. Identification of a Tri-Iron(III), Tri-Citrate Complex in the Xylem Sap of Iron-Deficient Tomato Resupplied with Iron: New Insights into Plant Iron Long-Distance Transport

Author:
Rellán-Álvarez, Rubén; Giner-Martínez-Sierra, Justo; Orduna, Jesús; Orera, Irene; Rodríguez-Castrillón, José Ángel; García-Alonso, José Ignacio; Abadía, Javier; Álvarez-Fernández, Ana
Source:
Plant & cell physiology 2010 v.51 no.1 pp. 91-102
ISSN:
0032-0781
Subject:
Solanum lycopersicum; citrates; mass spectrometry; molecular weight; sap; tomatoes; xylem
Abstract:
... The identification of Fe transport forms in plant xylem sap is crucial to the understanding of long-distance Fe transport processes in plants. Previous studies have proposed that Fe may be transported as an Fe-citrate complex in plant xylem sap, but such a complex has never been detected. In this study we report the first direct and unequivocal identification of a natural Fe complex in plant xylem ...
DOI:
10.1093/pcp/pcp170
PubMed:
19942594

59. Identification of biochemical changes in lactovegetarian urine using ¹H NMR spectroscopy and pattern recognition

Author:
Xu, Jingjing; Yang, Shuyu; Cai, Shuhui; Dong, Jiyang; Li, Xuejun; Chen, Zhong
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.4 pp. 1451-1463
ISSN:
1618-2642
Subject:
arginine; chronic diseases; citrates; creatinine; females; formates; gender differences; glycoproteins; humans; males; mannitol; metabolism; metabolites; metabolomics; molecular weight; multivariate analysis; nuclear magnetic resonance spectroscopy; omnivores; phenylalanine; principal component analysis; succinic acid; taurine; urine; vegetarian diet
Abstract:
... A vegetarian diet has been demonstrated to have a profound influence on human metabolism as well as to aid the prevention of several chronic diseases relative to an omnivorous diet. However, there have been no systematic metabolomic studies on all of the biochemical changes induced in human subjects by long-term vegetarianism. In this study, ¹H NMR spectroscopy in combination with multivariate sta ...
DOI:
10.1007/s00216-009-3338-z

60. Identification of three relationships linking cadmium accumulation to cadmium tolerance and zinc and citrate accumulation in lettuce

Author:
Zorrig, Walid; Rouached, Aïda; Shahzad, Zaigham; Abdelly, Chedly; Davidian, Jean-Claude; Berthomieu, Pierre
Source:
Journal of plant physiology 2010 v.167 no.15 pp. 1239-1247
ISSN:
0176-1617
Subject:
Lactuca sativa; cadmium; citrates; culture media; heavy metals; lettuce; models; potassium; roots; salads; shoots; weight loss; xylem vessels; zinc
Abstract:
... Lettuce (Lactuca sativa) is a plant species that shows high accumulation of cadmium, a toxic heavy metal. Lettuce is therefore a good model both for identifying determinants controlling cadmium accumulation in plant tissues and for developing breeding strategies aimed at limiting cadmium accumulation in edible tissues. In this work, 14-day-old plants from three lettuce varieties were grown for 8 d ...
DOI:
10.1016/j.jplph.2010.04.012
PubMed:
20576318

61. Implications of citrate concentration during the seeded growth synthesis of gold nanoparticles

Author:
Anna A. Volkert; Varuni Subramaniam; Amanda J. Haes
Source:
Chemical communications 2010 v.47 no.1 pp. 478-480
ISSN:
1364-548X
Subject:
Gibbs free energy; chemical reactions; citrates; nanogold; nanoparticles
Abstract:
... Increasing the citrate concentration during the seeded growth synthesis of gold nanoparticles yields materials with decreased aspect ratios and increased defect densities. The stability of these nanoparticles is attributed to variations in their overall Gibb's free energy. ...
DOI:
10.1039/c0cc02075c

62. In vitro activities of plant extracts on human Loa loa isolates and cytotoxicity for eukaryotic cells

Author:
Mengome, Line-Edwige; Akue, Jean Paul; Souza, Alain; Feuya Tchoua, Guy Raymond; Nsi Emvo, Edouard
Source:
Parasitology research 2010 v.107 no.3 pp. 643-650
ISSN:
0932-0113
Subject:
Hymenolepis; Loa loa; Lophira; Peperomia; Piptadeniastrum; Uapaca; Vernonia; Zanthoxylum; alkaloids; antibiotics; biphenyl; carbon dioxide; citrates; cytotoxicity; diethylcarbamazine; encephalitis; eukaryotic cells; flavonoids; humans; inhibitory concentration 50; ivermectin; microfilariae; patients; plant extracts; polyphenols; reducing sugars; screening; sterols; tetrazolium; triterpenoids
Abstract:
... Loa loa, a filarial worm, can cause fatal encephalitis in humans. In an attempt to find alternatives to the standard treatments (ivermectin and diethylcarbamazine citrate), we tested 12 methanolic extracts of nine traditional plant remedies. The extracts (100-0.09 µg/ml) were incubated with 20 Loa loa microfilariae isolated from patients at 37°C with 5% CO₂ in modified Eagle's medium supplemented ...
DOI:
10.1007/s00436-010-1910-2
PubMed:
20495930

63. Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis

Author:
Kim, Chai-Wan; Moon, Young-Ah; Park, Sahng Wook; Cheng, Dong; Kwon, Hyock Joo; Horton, Jay D.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.21 pp. 9626-9631
ISSN:
0027-8424
Subject:
acetyl-CoA carboxylase; citrates; dephosphorylation; electron microscopy; enzyme activity; fatty acids; fatty liver; gels; liver; mammals; phosphorylation; polymerization; triacylglycerols
Abstract:
... Acetyl-CoA carboxylase (ACC), the first committed enzyme in fatty acid (FA) synthesis, is regulated by phosphorylation/dephosphorylation, transcription, and an unusual mechanism of protein polymerization. Polymerization of ACC increases enzymatic activity and is induced in vitro by supraphysiological concentrations of citrate (> 5 mM). Here, we show that MIG12, a 22 kDa cytosolic protein of previo ...
DOI:
10.1073/pnas.1001292107
PubMed:
20457939
PubMed Central:
PMC2906888

64. Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism

Author:
Szal, Bożena; Jastrzębska, Agata; Kulka, Marek; Leśniak, Karolina; Podgórska, Anna; Pärnik, Tiit; Ivanova, Hiie; Keerberg, Olav; Gardeström, Per; Rychter, Anna M.
Source:
Planta 2010 v.232 no.6 pp. 1371-1382
ISSN:
0032-0935
Subject:
Cucumis sativus; amino acids; ammonium compounds; carbon; carbon dioxide; citrates; cucumbers; decarboxylation; electron transport chain; energy; leaves; mitochondrial genome; mutants; nitrates; nitrogen metabolism; pyruvic acid; starch; tricarboxylic acid cycle
Abstract:
... The MSC16 cucumber (Cucumis sativus L.) mitochondrial mutant was used to study the effect of mitochondrial dysfunction and disturbed subcellular redox state on leaf day/night carbon and nitrogen metabolism. We have shown that the mitochondrial dysfunction in MSC16 plants had no effect on photosynthetic CO₂ assimilation, but the concentration of soluble carbohydrates and starch was higher in leaves ...
DOI:
10.1007/s00425-010-1261-3
PubMed:
20830597
PubMed Central:
PMC2957574

65. Inorganic and Organic Solutes in Apoplastic and Symplastic Spaces Contribute to Osmotic Adjustment During Leaf Rolling in Ctenanthe Setosa

Author:
Aykut Saglam; Rabiye Terzi; Hatice Nar; Neslihan Saruhan; Faik Ayaz; Asim Kadioglu
Source:
Acta biologica Cracoviensia 2010 v.52 no.1 pp. 37-44
ISSN:
1898-0295
Subject:
Chamaeraphis; apoplast; calcium; chlorides; citrates; drought; glucose; ions; leaf rolling; leaves; malates; potassium; proline; rolling; sodium; solutes; sucrose; symplast; water stress
Abstract:
... In Ctenanthe setosa we studied changes in apoplastic and symplastic sugar, proline, ions and organic acids under drought stress causing leaf rolling. Leaf extractions were made at visually judged leaf rolling stages (not rolled, slightly rolled, strongly rolled, completely rolled). Glucose and sucrose content increased in the symplast. Glucose declined during leaf rolling in the apoplast, and sucr ...
DOI:
10.2478/v10182-010-0005-9

66. Intensification of surfactant synthesis in Rhodococcus erythropolis EK-1 cultivated on hexadecane

Author:
Pirog, T. P.; Shevchuk, T. A.; Klimenko, Yu. A.
Source:
Applied biochemistry and microbiology 2010 v.46 no.6 pp. 599-606
ISSN:
0003-6838
Subject:
Rhodococcus erythropolis; aldehyde dehydrogenase; cations; citrates; gluconeogenesis; hexadecane; iron; potassium; sodium; trehalose
Abstract:
... Activity of key enzymes of n-alkane metabolism was determined in cells of Rhodococcus erythropolis EK-1, a surfactant producer grown on n-hexadecane. Potassium cations were found to inhibit alkane hydroxylase and NADP⁺-dependent aldehyde dehydrogenase, while sodium cations were found to activate these enzymes. Decreased potassium concentration (to 1 mM), increased sodium concentration (to 35 mM), ...
DOI:
10.1134/S0003683810060074

67. Intra- and extra-cellular flux distributions of TCA and glyoxalate cycle and vancomycin production of Amycolatopsis orientalis grown in different glycerol concentration

Author:
Ayar-Kayali, H.; Tarhan, L.
Source:
Applied biochemistry and microbiology 2010 v.46 no.5 pp. 472-480
ISSN:
0003-6838
Subject:
Amycolatopsis orientalis; citrates; culture media; glycerol; isocitrate lyase; malates; metabolites; succinic acid; vancomycin
Abstract:
... The relationship between tricarboxylic acid (TCA) and glyoxalate cycle and the effect of their metabolites levels on the vancomycin production of Amycolatopsis orientalis were investigated in different concentration of glycerol (2.5-20 g/l). Intracellular glycerol levels increased with respect to increases in glycerol concentrations of the growth medium. Extracellular glycerol levels decreased slo ...
DOI:
10.1134/S0003683810050029

68. Isolation and characterisation of two MATE genes in rye

Author:
Yokosho, Kengo; Yamaji, Naoki; Ma, Jian Feng
Source:
Functional plant biology 2010 v.37 no.4 pp. 296-303
ISSN:
1445-4408
Subject:
Secale cereale; rye; Hordeum vulgare; barley; Oryza sativa; rice; roots; shoots; nutrient transport; gene expression regulation; aluminum; nutrient use efficiency; iron; nutrient deficiencies; genes; citrates; translocation (plant physiology)
Abstract:
... Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the detoxification of aluminium or translocation of iron from the roots to the shoots. In this study, we isolated two homologous genes (ScFRDL1 and ScFRDL2) from this family i ...
DOI:
10.1071/FP09265

69. Kinetics of oxidation of Cr(III)-organic complexes by H₂O₂

Author:
Zejiao Luo; Nivedita Chatterjee
Source:
Chemical speciation and bioavailability 2010 v.22 no.1 pp. 25-34
ISSN:
0954-2299
Subject:
EDTA (chelating agent); chromium; citrates; cysteine; glutamic acid; histidine; hydrogen peroxide; ligands; models; oxalates; oxidation; pH; pyruvic acid; serine
Abstract:
... Oxidation of Cr(III)-organic complexes by H₂O₂ was examined in laboratory scale experiments. Ascorbate, glutamate, histidine, cysteine, serine, mandelate, citrate, pyruvate, oxalate and EDTA were selected as model organic ligands. The oxidation of Cr(III)-organic complexes by H₂O₂ was first order with respect to [OH⁻] (pH 7–8.8), [H₂O₂] (from 0.0002 to 0.3 M), and [Cr(III)-L] (where L is the organ ...
DOI:
10.3184/095422909X12548400846521

70. Metabolic Alterations in Organic Acids and γ-Aminobutyric Acid in Developing Tomato (Solanum lycopersicum L.) Fruits

Author:
Yin, Yong-Gen; Tominaga, Takehiro; Iijima, Yoko; Aoki, Koh; Shibata, Daisuke; Ashihara, Hiroshi; Nishimura, Shigeo; Ezura, Hiroshi; Matsukura, Chiaki
Source:
Plant & cell physiology 2010 v.51 no.8 pp. 1300-1314
ISSN:
0032-0781
Subject:
Solanum lycopersicum; carbohydrate metabolism; cell physiology; citrates; early development; fruiting; malates; pericarp; phosphoenolpyruvate carboxylase; pyruvic acid; salt stress; succinic acid; tomatoes; tricarboxylic acid cycle
Abstract:
... Salt stress improves the quality of tomato fruits. To clarify the mechanism(s) underlying this phenomenon, we investigated metabolic alterations in tomato fruits exposed to 160 mM salt, focusing on metabolism of organic acids related to the tricarboxylic acid (TCA) cycle and γ-aminobutyric acid (GABA). Quantitative analyses revealed that most amino acids increased in response to salt stress throug ...
DOI:
10.1093/pcp/pcq090

71. Metabolic alterations in the hamster co-infected with Schistosoma japonicum and Necator americanus

Author:
Wu, Jun-Fang; Holmes, Elaine; Xue, Jian; Xiao, Shu-Hua; Singer, Burton H.; Tang, Hui-Ru; Utzinger, Jürg; Wang, Yu-Lan
Source:
International journal for parasitology 2010 v.40 no.6 pp. 695-703
ISSN:
0020-7519
Subject:
Necator americanus; Schistosoma japonicum; amino acids; animal models; blood serum; cercariae; citrates; digestive system; glucose; hamsters; hookworms; humans; metabolites; metabolomics; mixed infection; multivariate analysis; nuclear magnetic resonance spectroscopy; parasitoses; succinic acid; tricarboxylic acid cycle; urine; South America
Abstract:
... Co-infection with hookworm and schistosomes is a common phenomenon in sub-Saharan Africa, as well as in parts of South America and southeast Asia. As a first step towards understanding the metabolic response of a hookworm-schistosome co-infection in humans, we investigated the metabolic consequences of co-infection in an animal model, using a nuclear magnetic resonance (NMR)-based metabolic profil ...
DOI:
10.1016/j.ijpara.2009.11.003

72. Metabolic and histopathological alterations of Jatropha mosaic begomovirus-infected Jatropha curcas L. by HR-MAS NMR spectroscopy and magnetic resonance imaging

Author:
Sidhu, O. P.; Annarao, Sanjay; Pathre, Uday; Snehi, S. K.; Raj, S. K.; Roy, Raja; Tuli, Rakesh; Khetrapal, C. L.
Source:
Planta 2010 v.232 no.1 pp. 85-93
ISSN:
0032-0935
Subject:
Jatropha curcas; Jatropha mosaic virus; alanine; arginine; biotic stress; choline; citrates; gamma-aminobutyric acid; glucose; glutamine; histopathology; hyperplasia; leaves; lipids; magnetic resonance imaging; malates; metabolism; microscopy; nuclear magnetic resonance spectroscopy; photosynthates; pith; respiratory rate; sap; spatial variation; stem cells; stems; sucrose; tissues; tricarboxylic acid cycle; valine; water distribution
Abstract:
... Alterations in the anatomical structures, sap translocation and metabolic profiles in Jatropha curcas L. (Euphorbiaceae), infected with Jatropha mosaic virus (JMV) have been investigated using MRI and HR-MAS NMR spectroscopy. The contrast of MRI images distinguishes abnormalities in anatomical structures of infected and healthy stem. The HR-MAS NMR spectroscopic analysis indicated that viral infec ...
DOI:
10.1007/s00425-010-1159-0
PubMed:
20372923

73. Metabolic pathway of α-ketoglutarate in Lactobacillus sanfranciscensis and Lactobacillus reuteri during sourdough fermentation

Author:
Zhang, C.; Gänzle, M.G.
Source:
Journal of applied microbiology 2010 v.109 no.4 pp. 1301-1310
ISSN:
1364-5072
Subject:
Lactobacillus reuteri; Lactobacillus sanfranciscensis; NAD (coenzyme); alpha-ketoglutaric acid; biochemical pathways; citrates; fermentation; flavor; gas chromatography; high performance liquid chromatography; metabolites; phenylalanine; sourdough; transamination; wheat
Abstract:
... To identify metabolites of α-ketoglutarate (α-KG) in Lactobacillus sanfranciscensis and Lactobacillus reuteri in modified MRS and sourdough. Lactobacillus sanfranciscensis and L. reuteri were grown with additional α-KG in mMRS and in wheat sourdough. In mMRS, α-KG was used as an electron acceptor and converted to 2-hydroxyglutarate (2-OHG) by both organisms. Production of 2-OHG was identified by h ...
DOI:
10.1111/j.1365-2672.2010.04753.x
PubMed:
20477886

74. Migration analysis of epoxidized soybean oil and other plasticizers in commercial lids for food packaging by gas chromatography-mass spectrometry

Author:
Bueno-Ferrer, C.; Jimenez, A.; Garrigos, M.C.
Source:
Food additives & contaminants 2010 v.27 no.10 pp. 1469-1477
Subject:
food matrix; food packaging; lipids; adipic acid; food composition; mass spectrometry; food analysis; detection; model food systems; citrates; packaging materials; quantitative analysis; packaging materials migration; plasticizers; soybean oil; poly(vinyl chloride); epoxides; water; gas chromatography
Abstract:
... A modification of the method of Castle et al. (J. Chromatogr. 1988: 437:274-280) for the analysis of epoxidized soybean oil (ESBO) is proposed to simplify the analysis and reduce the time and consumption of reagents. The proposed modifications, particularly the elimination of the internal standard, resulted in a simpler, faster and more economical method. A complete analytical validation, includin ...

75. Mitochondrial enzymes and citrate transporter contribute to the aluminium-induced citrate secretion from soybean (Glycine max) roots

Author:
Xu, Muyun; You, Jiangfeng; Hou, Ningning; Zhang, Hongmei; Chen, Guang; Yang, Zhenming
Source:
Functional plant biology 2010 v.37 no.4 pp. 285-295
ISSN:
1445-4408
Subject:
Glycine max; soybeans; roots; aluminum; phytotoxicity; disease resistance; citrates; metabolism; transport proteins; mitochondria; malate dehydrogenase; citrate (si)-synthase; aconitate hydratase; enzyme activity; dose response; protein secretion; gene expression regulation; transcription (genetics); plant growth; acid soils
Abstract:
... The concentration of soluble aluminium (Al) in the soil solution increases at low pH and the prevalence of toxic Al³⁺ cations represent the main factor limiting plant growth on acid soils. Citrate secretion from roots is an important Al-tolerance mechanism in many species including soybean. We isolated mitochondria from the roots of an Al-resistant soybean (Glycine max L.) cv. Jiyu 70 to investiga ...
DOI:
10.1071/FP09223

76. Modelling rhizosphere transport in the presence of goethite, including competitive uptake of phosphate and arsenate

Author:
Szegedi, Krisztian; Vetterlein, Doris; Jahn, Reinhold
Source:
Plant and soil 2010 v.330 no.1-2 pp. 481-501
ISSN:
0032-079X
Subject:
Zea mays; arsenates; citrates; computers; goethite; models; nutrient availability; phosphates; rhizosphere; root exudates; soil; sorption
Abstract:
... A modelling approach was used to extend the knowledge about the processes that affect the availability of the nutrient P and the toxic agent AsV in the rhizosphere in the presence of a strong sorbent. Based on compartment system experiments in which Zea mays was grown the following hypothesis were assumed: a) measured P concentration gradients can be explained by the mobilisation of P by the root ...
DOI:
10.1007/s11104-009-0221-9

77. Monitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources

Author:
Wang, Qin; Nomura, Christopher T.
Source:
Journal of bioscience and bioengineering 2010 v.110 no.6 pp. 653-659
ISSN:
1389-1723
Subject:
ABC transporters; Pseudomonas putida; beta oxidation; biodegradability; bioremediation; biosynthesis; carbon; citrates; dodecanoic acid; enzymes; gene expression; genetically modified organisms; glucose; glycerol; long chain fatty acids; monitoring; plastics; polyhydroxyalkanoates; quantitative polymerase chain reaction; regulon; tricarboxylic acid cycle
Abstract:
... Pseudomonas putida has a variety of potential uses in bioremediation and biosynthesis of biodegradable plastics. P. putida is able to utilize a wide range of carbon sources. In this study, P. putida KT2440 was grown on glucose, glycerol, citrate, or fatty acid (lauric acid) as the sole carbon source. Differences in expression levels of genes involved in the Entner-Doudoroff pathway, glycerol metab ...
DOI:
10.1016/j.jbiosc.2010.08.001

78. Multiobjective evolutionary optimisation for surface-enhanced Raman scattering

Author:
Jarvis, Roger M.; Rowe, William; Yaffe, Nicola R.; O'Connor, Richard; Knowles, Joshua D.; Blanch, Ewan W.; Goodacre, Royston
Source:
Analytical and bioanalytical chemistry 2010 v.397 no.5 pp. 1893-1901
ISSN:
1618-2642
Subject:
Raman spectroscopy; algorithms; citrates; colloids; computer science; cysteine; experimental design; hydroxylamine; silver
Abstract:
... In most optimisation experiments, a single parameter is first optimised before a second and then third one are subsequently modified to give the best result. By contrast, we believe that simultaneous multiobjective optimisation is more powerful; therefore, an optimisation of the experimental conditions for the colloidal SERS detection of l-cysteine was carried out. Six aggregating agents and three ...
DOI:
10.1007/s00216-010-3739-z
PubMed:
20440481

79. Multiple adaptive responses of Australian native perennial legumes with pasture potential to grow in phosphorus- and moisture-limited environments

Author:
Suriyagoda, Lalith D.B.; Ryan, Megan H.; Renton, Michael; Lambers, Hans
Source:
Annals of botany 2010 v.105 no.5 pp. 755-767
ISSN:
0305-7364
Subject:
Cullen; Medicago sativa; alfalfa; citrates; climate; drought; drying; field capacity; forage legumes; greenhouses; leaf water potential; malates; nutrition; pastures; photosynthesis; root systems; shoots; stomatal conductance; water use efficiency
Abstract:
... BACKGROUND AND AIMS: Many Australian legumes have evolved in low-phosphorus (P) soils and low-rainfall areas. Therefore a study was made of the interaction of soil [P] and water availability on growth, photosynthesis, water-use efficiency (WUE) and P nutrition of two Australian native legumes with pasture potential, Cullen australasicum and C. pallidum, and the widely grown exotic pasture legume, ...
DOI:
10.1093/aob/mcq040
PubMed:
20421234
PubMed Central:
PMC2859915

80. Nitric oxide is involved in phosphorus deficiency-induced cluster-root development and citrate exudation in white lupin

Author:
Wang, B.L.; Tang, X.Y.; Cheng, L.Y.; Zhang, A.Z.; Zhang, W.H.; Zhang, F.S.; Liu, J.Q.; Cao, Y.; Allan, D.L.; Vance, C.P.; Shen, J.B.
Source:
New phytologist 2010 v.187 no.4 pp. 1112-1123
ISSN:
0028-646X
Subject:
nitric oxide; Lupinus albus; phosphorus; nutrient deficiencies; nitrous oxide; roots; root growth; citrates; exudation; developmental stages; biochemical pathways; forage legumes; gene expression
Abstract:
... White lupin (Lupinus albus) forms specialized cluster roots characterized by exudation of organic anions under phosphorus (P) deficiency. Here, the role of nitric oxide (NO) in P deficiency-induced cluster-root formation and citrate exudation was evaluated. White lupin plants were treated with the NO donor sodium nitroprusside (SNP) and scavenger or inhibitor of NO synthase under conditions of P d ...
Handle:
10113/47016
DOI:
10.1111/j.1469-8137.2010.03323.x
PubMed:
20553395

81. Nonreductive Iron Uptake Mechanism in the Marine Alveolate Chromera velia

Author:
Sutak, Robert; Šlapeta, Jan; San Roman, Mabel; Camadro, Jean-Michel; Lesuisse, Emmanuel
Source:
Plant physiology 2010 v.154 no.2 pp. 991-1000
ISSN:
0032-0889
Subject:
Saccharomyces cerevisiae; binding sites; cell walls; chelates; chelating agents; citrates; electron transfer; ions; iron; microalgae; models; oceans; photosynthesis; uptake mechanisms; yeasts
Abstract:
... Chromera velia is a newly cultured photosynthetic marine alveolate. This microalga has a high iron requirement for respiration and photosynthesis, although its natural environment contains less than 1 nM of this metal. We found that this organism uses a novel mechanism of iron uptake, differing from the classic reductive and siderophore-mediated iron uptake systems characterized in the model yeast ...
DOI:
10.1104/pp.110.159947
PubMed:
20724644
PubMed Central:
PMC2949016

82. Novel alternative to antibiotics in shrimp hatchery: effects of the essential oil of Cinnamosma fragrans on survival and bacterial concentration of Penaeus monodon larvae

Author:
R. Randrianarivelo; P. Danthu; C. Benoit; P. Ruez; M. Raherimandimby; S. Sarter
Source:
Journal of applied microbiology 2010 v.109 no.2 pp. 642-650
ISSN:
1364-5072
Subject:
Penaeus monodon; agar; bile; citrates; erythromycin; essential oils; farming systems; hatcheries; indigenous species; larvae; microbial load; minimum inhibitory concentration; oils; shrimp; sucrose; survival rate; thiosulfates; Madagascar
Abstract:
... Aims: The activity of two essential oils (EOs) of Cinnamosma fragrans, an endemic plant to Madagascar (B8: linalool‐type and B143: 1,8‐cineole‐type), against bacterial isolates from a shrimp hatchery of Penaeus monodon and their effects on the survival and bacterial concentration of larvae were determined. Methods and Results: Minimum inhibitory concentrations were determined using a broth dilutio ...
DOI:
10.1111/j.1365-2672.2010.04694.x
PubMed:
20180878

83. Nutrient and growth responses of cattail (Typha domingensis) to redox intensity and phosphate availability

Author:
Li, Shuwen; Lissner, Jørgen; Mendelssohn, Irving A.; Brix, Hans; Lorenzen, Bent; McKee, Karen L.; Miao, Shili
Source:
Annals of botany 2010 v.105 no.1 pp. 175-184
ISSN:
0305-7364
Subject:
Cladium mariscus subsp. jamaicense; citrates; nutrient content; redox potential; buffers; titanium; water conservation; nutrients; hydroponics; Typha domingensis; hydrology; leaves; phosphates; biomass; nutrient use efficiency; roots; conservation areas; phosphorus; Florida
Abstract:
... BACKGROUND AND AIMS: In the Florida Everglades, the expansion of cattail (Typha domingensis) into areas once dominated by sawgrass (Cladium jamaicense) has been attributed to altered hydrology and phosphorus (P) enrichment. The objective of this study was to quantify the interactive effects of P availability and soil redox potential (Eh) on the growth and nutrient responses of Typha, which may hel ...
DOI:
10.1093/aob/mcp213
PubMed:
19748907
PubMed Central:
PMC2794056

84. On-column silver substrate synthesis and surface-enhanced Raman detection in capillary electrophoresis

Author:
Leopold, Nicolae; Lendl, Bernhard
Source:
Analytical and bioanalytical chemistry 2010 v.396 no.6 pp. 2341-2348
ISSN:
1618-2642
Subject:
Raman spectroscopy; capillary electrophoresis; citrates; copper; desorption; methylene blue; monitoring; silica; silver; silver nitrate
Abstract:
... A new, simple, and efficient approach for on-column surface-enhanced Raman scattering (SERS) detection in capillary electrophoresis (CE) is reported. A ∼50-µm SERS substrate spot was prepared by laser-induced growth of silver particles in the 100-µm inner diameter CE capillary window or in a flow cell consisting of a 250-µm inner diameter fused silica capillary connector. For this purpose, the Ram ...
DOI:
10.1007/s00216-010-3468-3

85. Ordered ferrimagnetic form of ferrihydrite reveals links among structure, composition, and magnetism

Author:
Michel, F. Marc; Barrón, Vidal; Torrent, José; Morales, María P.; Serna, Carlos J.; Boily, Jean-François; Liu, Qingsong; Ambrosini, Andrea; Cismasu, A. Cristina; Brown, Gordon E. Jr.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.7 pp. 2787-2792
ISSN:
0027-8424
Subject:
ambient temperature; citrates; ferrihydrite; ferrimagnetic materials; ferritin; hematite; magnetism; models; particle size; soil; soil weathering
Abstract:
... The natural nanomineral ferrihydrite is an important component of many environmental and soil systems and has been implicated as the inorganic core of ferritin in biological systems. Knowledge of its basic structure, composition, and extent of structural disorder is essential for understanding its reactivity, stability, and magnetic behavior, as well as changes in these properties during aging. He ...
DOI:
10.1073/pnas.0910170107
PubMed:
20133643
PubMed Central:
PMC2840321

86. Organic acids differ in enhancing phosphorus uptake by Triticum aestivum L.—effects of rhizosphere concentration and counterion

Author:
Khademi, Z.; Jones, D. L.; Malakouti, M. J.; Asadi, F.
Source:
Plant and soil 2010 v.334 no.1-2 pp. 151-159
ISSN:
0032-079X
Subject:
Triticum aestivum; calcareous soils; citrates; mineralization; oxalates; phosphorus; potassium dihydrogen phosphate; rhizosphere; roots; shoots; wheat
Abstract:
... Organic acids such as citrate and oxalate have been implicated in enhancing many rhizosphere processes including nutrient acquisition. This study was conducted to determine the importance of organic acid type and concentration on rhizosphere P mobilization and subsequent uptake by wheat (Triticum aestivum L.) roots and its translocation to shoots. A single wheat plant was grown in soil-filled rhiz ...
DOI:
10.1007/s11104-009-0215-7

87. Pentavalent Arsenate Reductase Activity in Cytosolic Fractions of Pseudomonas sp., Isolated from Arsenic-Contaminated Sites of Tezpur, Assam

Author:
Deepti Srivastava; Datta Madamwar; R. B. Subramanian
Source:
Applied biochemistry and biotechnology 2010 v.162 no.3 pp. 766-779
ISSN:
0273-2289
Subject:
cytosol; microbial growth; strains; selenates; soil pollution; glucose; iron; bioremediation; cadmium; cell free system; citrates; soil bacteria; copper; calcium; arsenic; arsenates; temperature; enzyme activity; Pseudomonas; galactose; pH; India
Abstract:
... Pentavalent arsenate reductase activity was localized and characterized in vitro in the cytosolic fraction of a newly isolated bacterial strain from arsenic-contaminated sites. The bacterium was gram negative, rod-shaped, nonmotile, non-spore-forming, and noncapsulated, and the strain was identified as Pseudomonas sp. DRBS1 following biochemical and molecular approaches. The strain Pseudomonas sp. ...
DOI:
10.1007/s12010-009-8852-0
PubMed:
19950002

88. Photo-Fenton degradation of the herbicide tebuthiuron under solar irradiation: Iron complexation and initial intermediates

Author:
Silva, Milady R.A.; Vilegas, Wagner; Zanoni, Maria Valnice B.; Nogueira, Raquel F. Pupo
Source:
Water research 2010 v.44 no.12 pp. 3745-3753
ISSN:
0043-1354
Subject:
carbon; citrates; citric acid; hydroxylation; ions; iron; irradiation; oxidation; solar radiation; tebuthiuron; urea
Abstract:
... The complexation of iron ions with the herbicide tebuthiuron (TBH), during a solar photo-Fenton process, was investigated using cyclic voltammetry with a glassy carbon electrode. An oxidation peak was observed at +0.64 V after addition of Fe(NO3)3 to TBH solution, indicating the formation of a Fe-TBH complex, which was not observed in the presence of ferrioxalate or citrate complexes. This complex ...
DOI:
10.1016/j.watres.2010.04.025
PubMed:
20537676

89. Physicochemical Characterization of Creatine N-Methylguanidinium Salts

Author:
Brandon T. Gufford; Kamaraj Sriraghavan; Nicholas J. Miller; Donald W. Miller; Xiaochen Gu; Jonathan L. Vennerstrom; Dennis H. Robinson
Source:
Journal of dietary supplements 2010 v.7 no.3 pp. 240-252
ISSN:
1939-022X
Subject:
athletic performance; citrates; creatine; dietary supplements; gastrointestinal system; guanidinium; hydrogen; isoelectric point; maleates; pH; permeability; pyruvic acid; therapeutics; water solubility; zwitterions
Abstract:
... Creatine is widely used as a dietary supplement for body builders to enhance athletic performance. As the monohydrate, its low solubility in water and high dose lead to water retention and gastrointestinal discomfort. Hence, alternative creatine derivatives with enhanced water solubility and potential therapeutic advantages have been synthesized. As a zwitterionic compound, creatine can form salts ...
DOI:
10.3109/19390211.2010.491507

90. The Plackett-Burman design in optimization of media components for biomass production of Lactobacillus rhamnosus OXY

Author:
A. Waśko; Monika Kordowska-Wiater; M. Podleśny; Magdalena Polak-Berecka; Z. Targoński; Agnieszka Kubik-Komar
Source:
Acta biologica Hungarica 2010 v.61 no.3 pp. 344-355
ISSN:
0236-5383
Subject:
Lactobacillus rhamnosus; amino acids; bacteria; biomass production; bioreactors; carbon; citrates; cost effectiveness; culture media; glucose; growth factors; meat extracts; models; nitrogen; potassium phosphates; probiotics; pyruvic acid; sodium; sodium acetate; vitamin B complex
Abstract:
... The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, mea ...
DOI:
10.1556/ABiol.61.2010.3.10

91. Plant Growth Promotion by an Extracellular HAP-Phytase of a Thermophilic Mold Sporotrichum thermophile

Author:
Singh, Bijender; Satyanarayana, T.
Source:
Applied biochemistry and biotechnology 2010 v.160 no.5 pp. 1267-1276
ISSN:
0273-2289
Subject:
plant growth substances; Myceliophthora thermophila; molds (fungi); phytases; phosphates; phytic acid; calcium; magnesium; cobalt; aluminum; iron; zinc; enzymatic hydrolysis; temperature; citrates; wheat; fungal spores; plant growth; composts; wheat straw; soil fungi
Abstract:
... Phytase of the thermophilic mold Sporotrichum thermophile Apinis hydrolyzed and liberated inorganic phosphate from Ca⁺², Mg⁺², and Co⁺² phytates more efficiently than those of Al³⁺, Fe²⁺, Fe³⁺, and Zn²⁺. The hydrolysis rate was higher at 60 °C as compared to 26 °C. Among all the organic acids tested, citrate was more effective in enhancing solubilization of insoluble phytate salts by phytase than ...
DOI:
10.1007/s12010-009-8593-0
PubMed:
19333564

92. Plant Uptake of Depleted Uranium from Manure-Amended and Citrate Treated Soil

Author:
Sevostianova, Elena; Lindemann, William C.; Ulery, April L.; Remmenga, Marta D.
Source:
International journal of phytoremediation 2010 v.12 no.6 pp. 550-561
ISSN:
1522-6514
Subject:
Amaranthus retroflexus; Bassia scoparia; Helianthus annuus; Zea mays; aboveground biomass; acidification; citrates; citric acid; glyphosate; greenhouse experimentation; growing season; leaves; phytoremediation; polluted soils; solubilization; steers; stems; sweetcorn; uranium; New Mexico
Abstract:
... Six plant species were tested for their ability to accumulate depleted uranium in their aboveground biomass from deployed munitions contaminated soil in New Mexico. In greenhouse experiments, Kochia (Kochia scoparia L. Schrad.) and pigweed (Amaranthus retroflexus L.) were grown with steer manure added at rates of 22.4, 44.8, and 89.6 Mg ha-1. Citric acid and glyphosate (N-(phosphonomethyl) glycine ...
DOI:
10.1080/15226510903353153

93. Predicting the occurrence of iron chlorosis in grapevine with tests based on soil iron forms

Author:
Isabel Díaz de la Torre; María del Carmen Del Campillo; Vidal Barrón; José Torrent
Source:
OENO One 2010 v.44 no.2 pp. 77-85
ISSN:
2494-1271
Subject:
Vitis; alkalinity; ammonium; ammonium oxalate; calcite; calcium carbonate; chlorophyll; chlorosis; citrates; clearcutting; hydroxylamine; leaves; models; plant growth; risk assessment; rootstocks; vineyards
Abstract:
... Aims: Iron deficiency symptoms (leaf chlorosis and depressed growth) are common in grapevine growing on calcareous soils. The objective of this study was to investigate the relationships between these symptoms and the properties of Spanish vineyard soils. Methods and results: Soils from thirty vineyards with leaf chlorosis were used to pot '110 Richter' rootstock for three seasons. Mean leaf chlor ...
DOI:
10.20870/oeno-one.2010.44.2.1463

94. Production of bacterial cellulose from Enterobacter amnigenus GH-1 isolated from rotten apple

Author:
Hungund, Basavaraj S.; Gupta, S. G.
Source:
World journal of microbiology & biotechnology 2010 v.26 no.10 pp. 1823-1828
ISSN:
0959-3993
Subject:
Enterobacter amnigenus; apples; biosensors; calcium; carbon; casein hydrolysates; cellulose; citrates; coconut milk; coconut water; ethanol; fabrics; fermentation; fructose; hydrolysates; industry; magnesium; metal ions; methanol; molasses; orange juice; pH; phosphates; pineapple juice; pomegranates; production costs; solvents; starch; sugarcane juice; temperature; yeast extract; zinc
Abstract:
... Bacterial cellulose finds novel applications in biomedical, biosensor, food, textile and other industries. The optimum fermentation conditions for the production of cellulose by newly isolated Enterobacter amnigenus GH-1 were investigated. The strain was able to produce cellulose at temperature 25-35°C with a maximum at 28°C. Cellulose production occurred at pH 4.0-7.0 with a maximum at 6.5. After ...
DOI:
10.1007/s11274-010-0363-1

95. Proposal of a simple screening method for a rapid preliminary evaluation of “heavy metals” mobility in soils of contaminated sites

Author:
Pinto, Valentina; Chiusolo, Francesca; Cremisini, Carlo
Source:
Journal of soils and sediments 2010 v.10 no.6 pp. 1115-1122
ISSN:
1439-0108
Subject:
buffers; citrates; heavy metals; hydroxides; ions; leaching; mineralization; monitoring; oxides; pH; polluted soils; rapid methods; remediation; risk; screening; soil pollution; soil sampling; titration; urban areas
Abstract:
... Purpose Risks associated to “heavy metals” (HM) soil contamination depend not only on their total content but, mostly, on their mobility. Many extraction procedures have been developed to evaluate HM mobility in contaminated soils, but they are generally time consuming (especially the sequential extraction procedures (SEPs)) and consequently applicable on a limited number of samples. For this reas ...
DOI:
10.1007/s11368-010-0208-4

96. Pseudomonas fluorescens orchestrates a fine metabolic-balancing act to counter aluminium toxicity

Author:
Lemire, Joseph; Mailloux, Ryan; Auger, Christopher; Whalen, Daniel; Appanna, Vasu D.
Source:
Environmental microbiology 2010 v.12 no.6 pp. 1384-1390
ISSN:
1462-2912
Subject:
NAD (coenzyme); citrates; Pseudomonas fluorescens; iron; application technology; oxidative phosphorylation; adenosine triphosphate; aerobiosis; metabolites; carbon; microorganisms; aluminum; homeostasis; toxins; toxicity; aconitate hydratase; tricarboxylic acids; isocitrate lyase
Abstract:
... Aluminium (Al), an environmental toxin, is known to disrupt cellular functions by perturbing iron (Fe) homeostasis. However, Fe is essential for such metabolic processes as the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, the two pivotal networks that mediate ATP production during aerobiosis. To counter the Fe conundrum induced by Al toxicity, Pseudomonas fluorescens utilizes isoc ...
DOI:
10.1111/j.1462-2920.2010.02200.x
PubMed:
20353438

97. Purification and Characterization of OmcZ, an Outer-Surface, Octaheme c-Type Cytochrome Essential for Optimal Current Production by Geobacter sulfurreducens

Author:
Inoue, Kengo; Qian, Xinlei; Morgado, Leonor; Kim, Byoung-Chan; Mester, Tünde; Izallalen, Mounir; Salgueiro, Carlos A.; Lovley, Derek R.
Source:
Applied and environmental microbiology 2010 v.76 no.12 pp. 3999-4007
ISSN:
0099-2240
Subject:
Geobacter sulfurreducens; amino acid sequences; biofilm; citrates; electrodes; electron transfer; electrons; heme; humic substances; hydrogen; microbial fuel cells; molecular weight; oxides; proteins; sequence analysis; temperature; titration
Abstract:
... Previous studies have demonstrated that Geobacter sulfurreducens requires the c-type cytochrome OmcZ, which is present in large (OmcZL; 50-kDa) and small (OmcZS; 30-kDa) forms, for optimal current production in microbial fuel cells. This protein was further characterized to aid in understanding its role in current production. Subcellular-localization studies suggested that OmcZS was the predominan ...
DOI:
10.1128/AEM.00027-10
PubMed:
20400562
PubMed Central:
PMC2893489

98. Replacement of traditional emulsifying salts by selected hydrocolloids in processed cheese production

Author:
Černíková, Michaela; Buňka, František; Pospiech, Matej; Tremlová, Bohuslava; Hladká, Kristýna; Pavlínek, Vladimír; Březina, Pavel
Source:
International dairy journal 2010 v.20 no.5 pp. 336-343
ISSN:
0958-6946
Subject:
modified starch; cheesemaking; microstructure; citrates; model food systems; dry matter content; texture; pectins; hydrocolloids; sensory properties; carrageenan; emulsifiers; rheological properties; phosphates; locust bean gum; processed cheeses
Abstract:
... Different hydrocolloids were examined as possible replacements for traditional phosphate- and citrate-based emulsifying salts in processed cheese production. The following hydrocolloids (at concentrations in the final product of ≤1.0%, w/w) were chosen: modified starch (with bound sodium octenyl succinate), low methoxyl pectin (alone or combined with lecithin), locust bean gum, κ-carrageenan and ι ...
DOI:
10.1016/j.idairyj.2009.12.012

99. Requirement of the Lactobacillus casei MaeKR Two-Component System for L-Malic Acid Utilization via a Malic Enzyme Pathway

Author:
Landete, José María; García-Haro, Luisa; Blasco, Amalia; Manzanares, Paloma; Berbegal, Carmen; Monedero, Vicente; Zúñiga, Manuel
Source:
Applied and environmental microbiology 2010 v.76 no.1 pp. 84-95
ISSN:
0099-2240
Subject:
Enterococcus faecalis; Lactobacillus casei; Streptococcus agalactiae; Streptococcus pyogenes; Streptococcus uberis; carbon; citrates; deoxyribonuclease I; gene deletion; glucose; histidine kinase; malic enzyme; malolactic fermentation; operon; promoter regions; transporters
Abstract:
... Lactobacillus casei can metabolize L-malic acid via malolactic enzyme (malolactic fermentation [MLF]) or malic enzyme (ME). Whereas utilization of L-malic acid via MLF does not support growth, the ME pathway enables L. casei to grow on L-malic acid. In this work, we have identified in the genomes of L. casei strains BL23 and ATCC 334 a cluster consisting of two diverging operons, maePE and maeKR, ...
DOI:
10.1128/AEM.02145-09
PubMed:
19897756
PubMed Central:
PMC2798650

100. Role of GlnR in Acid-Mediated Repression of Genes Encoding Proteins Involved in Glutamine and Glutamate Metabolism in Streptococcus mutans

Author:
Chen, Pei-Min; Chen, Yi-Ywan M.; Yu, Sung-Liang; Sher, Singh; Lai, Chern-Hsiung; Chia, Jean-San
Source:
Applied and environmental microbiology 2010 v.76 no.8 pp. 2478-2486
ISSN:
0099-2240
Subject:
Streptococcus mutans; acid tolerance; acid treatment; adenosine triphosphate; citrates; enzymes; glutamates; glutamine; metabolism; multigene family; mutants; operon; pH; proteins; regulon; reverse transcriptase polymerase chain reaction; survival rate
Abstract:
... The acid tolerance response (ATR) is one of the major virulence traits of Streptococcus mutans. In this study, the role of GlnR in acid-mediated gene repression that affects the adaptive ATR in S. mutans was investigated. Using a whole-genome microarray and in silico analyses, we demonstrated that GlnR and the GlnR box (ATGTNAN₇TNACAT) were involved in the transcriptional repression of clusters of ...
DOI:
10.1128/AEM.02622-09
PubMed:
20173059
PubMed Central:
PMC2849205