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You searched for: Publication year rev 7990-2010 Remove constraint Publication year rev: 7990-2010 Subject cycloheximide Remove constraint Subject: cycloheximide
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1. IL- 4-induced transcription factor NFIL3/E4BP4 controls IgE class switching

Author:
Kashiwada, Masaki; Levy, Deborah M.; McKeag, Lisa; Murray, Keri; Schröder, Andreas J.; Canfield, Stephen M.; Traver, Geri; Rothman, Paul B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.2 pp. 821-826
ISSN:
0027-8424
Subject:
B-lymphocytes; cycloheximide; genes; immunoglobulin E; interleukin-4; mice; transcription factors
Abstract:
... IL-4 signaling promotes IgE class switching through STAT6 activation and the induction of Ig germ-line ε (GLε) transcription. Previously, we and others identified a transcription factor, Nfil3, as a gene induced by IL-4 stimulation in B cells. However, the precise roles of nuclear factor, IL-3-regulated (NFIL3) in IL-4 signaling are unknown. Here, we report that NFIL3 is important for IgE class sw ...
DOI:
10.1073/pnas.0909235107
PubMed:
20080759
PubMed Central:
PMC2818942

2. Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

Author:
Rosenberger, Simone; Arce, Johanna De-Castro; Langbein, Lutz; Steenbergen, Renske D.M.; Rösl, Frank
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.15 pp. 7006-7011
ISSN:
0027-8424
Subject:
Papillomaviridae; RNA transport; alternative splicing; cycloheximide; epidermal growth factor; epidermal growth factor receptors; epithelium; exons; humans; messenger RNA; mitogen-activated protein kinase; neutralizing antibodies; open reading frames; protein synthesis; signal transduction; small interfering RNA; translation (genetics); uterine cervical neoplasms
Abstract:
... Certain types of human papillomaviruses (HPVs) are etiologically linked to cervical cancer. Their transforming capacity is encoded by a polycistronic premRNA, where alternative splicing leads to the translation of functional distinct proteins such as E6, E6*, and E7. Here we show that splicing of HPV16 E6/E7 ORF cassette is regulated by the epidermal growth factor (EGF) pathway. The presence of EG ...
DOI:
10.1073/pnas.1002620107
PubMed:
20351270
PubMed Central:
PMC2872467

3. Arabidopsis sensitivity to protein synthesis inhibitors depends on 26S proteasome activity

Author:
Kurepa, Jasmina; Karangwa, Consolée; Duke, Liliana Sfichi; Smalle, Jan A.
Source:
Plant cell reports 2010 v.29 no.3 pp. 249-259
ISSN:
0721-7714
Subject:
Arabidopsis; cycloheximide; herbicide resistance; herbicides; hygromycin B; mutants; phenotype; proteasome endopeptidase complex; protein synthesis inhibitors; proteinases; proteins; proteolysis; stress response; translation (genetics)
Abstract:
... The 26S proteasome (26SP), the central protease of the ubiquitin-dependent proteolysis pathway, controls the regulated proteolysis of functional proteins and the removal of misfolded and damaged proteins. In Arabidopsis, cellular and stress response phenotypes of a number of mutants with partially impaired 26SP function have been reported. Here, we describe the responses of proteasome mutants to p ...
DOI:
10.1007/s00299-010-0818-8
PubMed:
20087596

4. CTC medium: A novel dodine-free selective medium for isolating entomopathogenic fungi, especially Metarhizium acridum, from soil

Author:
Fernandes, Éverton K.K.; Keyser, Chad A.; Rangel, Drauzio E.N.; Foster, R. Nelson; Roberts, Donald W.
Source:
Biological control 2010 v.54 no.3 pp. 197-205
ISSN:
1049-9644
Subject:
Beauveria; soil fungi; chloramphenicol; interspecific variation; conidia; sporulation; entomopathogenic fungi; microbial growth; dodine; thiabendazole; culture media; Metarhizium; cycloheximide
Abstract:
... The selective media most commonly used for isolating hyphomycetous species of entomopathogenic fungi from non-sterile substrates rely on N-dodecylguanidine monoacetate (dodine) as the selective fungicide. Although these media are effective for isolating many species of Metarhizium and Beauveria from soil, they are inefficient media for isolation of an important Metarhizium species, Metarhizium acr ...
Handle:
10113/45048
DOI:
10.1016/j.biocontrol.2010.05.009

5. Chemical induction of rapid and reversible plastid filamentation in Arabidopsis thaliana roots

Author:
Itoh, Ryuuichi D.; Yamasaki, Hideo; Septiana, Andi; Yoshida, Shigeo; Fujiwara, Makoto T.
Source:
Physiologia plantarum 2010 v.139 no.2 pp. 144-158
ISSN:
0031-9317
Subject:
Arabidopsis thaliana; acids; antimycin A; chemical treatment; chloramphenicol; cortex; cotyledons; cyanides; cycloheximide; hydrogen peroxide; hypocotyls; leaves; mitochondria; morphogenesis; mutants; nitric oxide; plastids; protein synthesis inhibitors; roots; rotenone
Abstract:
... Plastids assume various morphologies depending on their developmental status, but the basis for developmentally regulated plastid morphogenesis is poorly understood. Chemical induction of alterations in plastid morphology would be a useful tool for studying this; however, no such chemicals have been identified. Here, we show that antimycin A, an effective respiratory inhibitor, can change plastid ...
DOI:
10.1111/j.1399-3054.2010.01352.x
PubMed:
20088905

6. Chlamydia pneumoniae Infection in Polarized Epithelial Cell Lines

Author:
Törmäkangas, Liisa; Markkula, Eveliina; Lounatmaa, Kari; Puolakkainen, Mirja
Source:
Infection and immunity 2010 v.78 no.6 pp. 2714-2722
ISSN:
0019-9567
Subject:
Chlamydophila pneumoniae; adenosinetriphosphatase; cell culture; cycloheximide; cytokines; doxycycline; epithelial cells; fluorescent antibody technique; genome; models; pathogenicity; pathogens; progeny; respiratory system; secretion; tight junctions
Abstract:
... We set up a polarized cell culture model to study the pathogenicity of a common respiratory tract pathogen, Chlamydia pneumoniae. Immunofluorescence staining of ZO-1 (a tight junction protein) and Na⁺K⁺ ATPase (a protein pump localized at the basolateral membrane in the polarized epithelial cells), as well as TER measurements, suggested that the filter-grown Calu-3 cells, but not the A549 cells, w ...
DOI:
10.1128/IAI.01456-09
PubMed:
20351147
PubMed Central:
PMC2876563

7. Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures

Author:
Doyle, Siamsa M.; Diamond, Mark; McCabe, Paul F.
Source:
Journal of experimental botany 2010 v.61 no.2 pp. 473-482
ISSN:
0022-0957
Subject:
Arabidopsis thaliana; viability; spectinomycin; cell death; protein synthesis; animals; reactive oxygen species; lincomycin; cell suspension culture; norflurazon; heat treatment; chloroplasts; cycloheximide
Abstract:
... Chloroplasts produce reactive oxygen species (ROS) during cellular stress. ROS are known to act as regulators of programmed cell death (PCD) in plant and animal cells, so it is possible that chloroplasts have a role in regulating PCD in green tissue. Arabidopsis thaliana cell suspension cultures are model systems in which to test this, as here it is shown that their cells contain well-developed, f ...
DOI:
10.1093/jxb/erp320
PubMed:
19933317
PubMed Central:
PMC2803215

8. Citrate transporters play an important role in regulating aluminum-induced citrate secretion in Glycine max

Author:
You, J. F.; Hou, N. N.; Xu, M. Y.; Zhang, H. M.; Yang, Z. M.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 766-768
ISSN:
0006-3134
Subject:
Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein synthesis; protein synthesis inhibitors; pyridoxal; roots; secretion; soybeans; transporters
Abstract:
... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
DOI:
10.1007/s10535-010-0138-4

9. Connexin32 hemichannels contribute to the apoptotic-to-necrotic transition during Fas-mediated hepatocyte cell death

Author:
Mathieu Vinken; Elke Decrock; Elke De Vuyst; Marijke De Bock; Roosmarijn E. Vandenbroucke; Bruno G. De Geest; Joseph Demeester; Niek N. Sanders; Tamara Vanhaecke; Luc Leybaert; Vera Rogiers
Source:
Cellular and molecular life sciences 2010 v.67 no.6 pp. 907-918
ISSN:
1420-682X
Subject:
apoptosis; cycloheximide; transcription (genetics)
Abstract:
... The present study was set up to investigate the fate of connexin32 and its channels in hepatocellular apoptosis. Primary hepatocyte cultures were exposed to Fas ligand and cycloheximide, and modifications in connexin32 expression and localization, and gap junction functionality were studied. We found that gap junction functionality rapidly declined upon progression of cell death, which was associa ...
DOI:
10.1007/s00018-009-0220-2

10. Cycloheximide speeds the porcine oocyte nuclear kinetics and retains embryonic developmental potential in the presence of gonadotrophins

Author:
Sun, Xu-Lei; Ma, Wen-Zhi; Zhu, Yu-Bo; Wu, Zhong-Hong; An, Lei; Chu, Da-Peng; Clapper, Jeffrey A.; Li, Ying; Tian, Jian-Hui
Source:
Canadian journal of animal science 2010 v.90 no.2 pp. 189-196
ISSN:
0008-3984
Subject:
cell cleavage; germinal vesicle; equine chorionic gonadotropin; cycloheximide; culture media; blastocyst; cultured cells; cell nucleus; human chorionic gonadotropin; cumulus oophorus; meiosis; metaphase; swine
Abstract:
... Animal embryo engineering requires large amounts of synchronized mature oocytes in vitro. However, porcine cumulus-oocyte complexes aspirated from 3-8 mm follicles are at different germinal vesicle stages. They reach metaphase II stages asynchronously when cultured in vitro. In this study, we examined the effects of pretreatment with or without cycloheximide (CHX), equine chorionic gonadotrophin ( ...

11. Detection of replicating negative-sense RNAs in CaCo-2 cells infected with human astrovirus

Author:
Jang, So Young; Jeong, Weon Hwa; Kim, Mi Suk; Lee, Young Min; Lee, Jae In; Lee, Gyu Cheol; Paik, Soon Young; Koh, Gwang Pyo; Kim, Jong Min; Lee, Chan Hee
Source:
Archives of virology 2010 v.155 no.9 pp. 1383-1389
ISSN:
0304-8608
Subject:
Human astrovirus; RNA; cycloheximide; diarrhea; gastroenteritis; genome; human cell lines; infants; pathogenicity; polymerase chain reaction; protein synthesis; reverse transcription; viruses
Abstract:
... Human astrovirus (HAstV) is the second most important cause of viral diarrhea and acute gastroenteritis in infants under five. However, determination of the infectivity of clinical isolates is difficult, and the replication cycle of HAstV is not yet fully understood. In this study, it was attempted to detect negative-sense (−)RNAs generated during the replication of RNA viruses. We used clinical i ...
DOI:
10.1007/s00705-010-0718-9
PubMed:
20544235

12. Development of a pilot‐scale 1 for Legionella elimination in biofilm in hot water network: heat shock treatment evaluation

Author:
M. Farhat; M.‐C. Trouilhé; E. Briand; M. Moletta‐Denat; E. Robine; J. Frère
Source:
Journal of applied microbiology 2010 v.108 no.3 pp. 1073-1082
ISSN:
1364-5072
Subject:
4',6-diamidino-2-phenylindole; Legionella; biofilm; cycloheximide; disinfection; heat; managers; polymerase chain reaction; tap water; vancomycin; France
Abstract:
... Aims: (i) To develop an analytical tool in order to evaluate the effectiveness of anti‐Legionella treatment in biofilm and (ii) study the fate of Legionella populations in water and biofilm after applying a heat shock treatment. Methods and Results: A pilot‐scale unit simulating a hot water system was built and designed by the Scientific and Technical Building Centre (CSTB, France). At the end of ...
DOI:
10.1111/j.1365-2672.2009.04541.x
PubMed:
19796094

13. Diallyl disulfide and diallyl trisulfide protect endothelial nitric oxide synthase against damage by oxidized low-density lipoprotein

Author:
Lei, Yen-Ping; Liu, Cheng-Tzu; Sheen, Lee-Yan; Chen, Haw-Wen; Lii, Chong-Kuei
Source:
Molecular nutrition & food research 2010 v.54 no.S1 pp. S42
ISSN:
1613-4125
Subject:
1-phosphatidylinositol 4-kinase; atherosclerosis; bioavailability; cyclic GMP; cycloheximide; garlic; health foods; low density lipoprotein; nitric oxide; nitric oxide synthase; phosphorylation; proteasome endopeptidase complex; protein content; protein degradation; protein synthesis; sulfides
Abstract:
... Garlic is viewed as an effective health food against atherosclerosis. In this study, we examined whether diallyl disulfide (DADS) and diallyl trisulfide (DATS) protect endothelial nitric oxide synthase (eNOS) activation against oxidized LDL (ox-LDL) insult and through what mechanism. We found that DADS and DATS reversed the suppression of eNOS Ser1177 phosphorylation by ox-LDL, and wortmannin abol ...
DOI:
10.1002/mnfr.200900278
PubMed:
20229525

14. Effect of cycloheximide, iodoacetamide, and antimycin A on inorganic phosphate synthesis in Saccharomyces cerevisiae VKM Y-1173

Author:
Trilisenko, L. V.; Kochetkova, O. Yu.; Vagabov, V. M.; Kulaev, I. S.
Source:
Microbiology 2010 v.79 no.1 pp. 23-29
ISSN:
0026-2617
Subject:
Saccharomyces cerevisiae; adenosine triphosphate; aeration; antimycin A; cycloheximide; ethanol; glucose; glycolysis; hypoxia; indole acetic acid; oxidative phosphorylation; phosphates; protein synthesis inhibitors; yeasts
Abstract:
... The effect of inhibitors of protein synthesis (cycloheximide, CHI), glycolysis (iodoacetamide, IAA), and oxidative phosphorylation (antimycin A, ANM) on inorganic phosphate (polyP) synthesis during the first 0.5 h of their hypercompensation in Saccharomyces cerevisiae VKM Y-l173 grown on 2% glucose-containing media at low (hypoxia) or high aeration rates or in the presence of 1 vol % ethanol under ...
DOI:
10.1134/S0026261710010030

15. Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora

Author:
Mancilla, Rodrigo A.; Canessa, Paulo; Manubens, Augusto; Vicuña, Rafael
Source:
Fungal genetics and biology 2010 v.47 no.7 pp. 656-661
ISSN:
1087-1845
Subject:
Ceriporiopsis subvermispora; manganese peroxidase; manganese; protein secretion; ligninolytic microorganisms; laccase; enzyme activity; messenger RNA; culture media; Western blotting; nucleic acid hybridization; cycloheximide
DOI:
10.1016/j.fgb.2010.04.003
PubMed:
20434578

16. Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742

Author:
Sitanggang, Azis Boing; Wu, Ho-Shing; Wang, Shaw S.; Ho, Yi-Cheng
Source:
Bioresource technology 2010 v.101 no.10 pp. 3595-3601
ISSN:
0960-8524
Subject:
glucosamine; pellets; Aspergillus; microbial physiology; submerged fermentation; culture media; chemical composition; glutamic acid; cycloheximide; methanol; ethanol
Abstract:
... The higher GlcN production using a wild-type fungi, Aspergillus sp. BCRC 31742 cultivated under submerged fermentation was investigated. Several fermentation aspects were studied, such as pellet size, working volume, agitation rate and stimulating factor. Culture cultivation with conditions, such as pellet diameter of 2.15mm, 50mL working volume (250mL T-flask), incubation at 30°C, 200rpm and pH 7 ...
DOI:
10.1016/j.biortech.2009.12.084
PubMed:
20093019

17. Effect of redox conditions on bacterial and fungal biomass and carbon dioxide production in Louisiana coastal swamp forest sediment

Author:
Seo, Dong Cheol; DeLaune, Ronald D.
Source:
Science of the total environment 2010 v.408 no.17 pp. 3623-3631
ISSN:
0048-9697
Subject:
bacteria; carbon dioxide; cycloheximide; forests; fungi; global warming; greenhouse effect; microbial biomass; redox potential; sea level; sediments; streptomycin; swamps; wastewater treatment; wetland soils; Louisiana
Abstract:
... Fungal and bacterial carbon dioxide (CO₂) production/emission was determined under a range of redox conditions in sediment from a Louisiana swamp forest used for wastewater treatment. Sediment was incubated in microcosms at 6 Eh levels (-200, -100, 0, +100, +250 and +400mV) covering the anaerobic range found in wetland soil and sediment. Carbon dioxide production was determined by the substrate-in ...
DOI:
10.1016/j.scitotenv.2010.04.043
PubMed:
20553938

18. Effects of autochthonous microbial community on the die-off of fecal indicators in tropical beach sand

Author:
Feng, Fan; Goto, Dustin; Yan, Tao
Source:
FEMS microbiology ecology 2010 v.74 no.1 pp. 214-225
ISSN:
0168-6496
Subject:
Enterococcus faecalis; Escherichia coli; Protozoa; bacteria; bacterial communities; bacteriophages; biotic stress; cycloheximide; die-off; gel electrophoresis; monitoring; predation; sand
Abstract:
... The recently observed high levels of fecal indicators in beach sand confound beach water monitoring efforts. The high levels of fecal indicators may be caused by the loss or the reduced activities of common environmental stresses controlling die-off in the sand. Microcosm experiments were conducted to compare the effects of biotic stresses from autochthonous sand bacteria, protozoa, and viruses on ...
DOI:
10.1111/j.1574-6941.2010.00921.x
PubMed:
20629750

19. Eicosapentaenoic acid up-regulates apelin secretion and gene expression in 3T3-L1 adipocytes

Author:
Lorente-Cebrián, Silvia; Bustos, Matilde; Marti, Amelia; Martinez, José Alfredo; Moreno-Aliaga, María Jesús
Source:
Molecular nutrition & food research 2010 v.54 no.S1 pp. S104
ISSN:
1613-4125
Subject:
actinomycin D; adipocytes; cycloheximide; eicosapentaenoic acid; gene expression; glucose tolerance; in vitro studies; messenger RNA; mice; phosphatidylinositol 3-kinase; secretion
Abstract:
... Recent studies have shown the ability of apelin to restore glucose tolerance in obese and insulin-resistant mice. Eicosapentaenoic acid (EPA) is a polyunsaturated fatty acid (PUFA) from the omega-3 family that has many beneficial effects in obesity-linked disorders. The aim of this study was to examine in vitro the effects of EPA on apelin secretion and gene expression in mature 3T3-L1 adipocytes. ...
DOI:
10.1002/mnfr.200900522
PubMed:
20352620

20. Exogenously induced expression of ethylene biosynthesis, ethylene perception, phospholipase D, and Rboh-oxidase genes in broccoli seedlings

Author:
Jakubowicz, Małgorzata; Gałgańska, Hanna; Nowak, Witold; Sadowski, Jan
Source:
Journal of experimental botany 2010 v.61 no.12 pp. 3475-3491
ISSN:
0022-0957
Subject:
1-aminocyclopropane-1-carboxylic acid; Brassica oleracea; acids; broccoli; cycloheximide; ethylene; ethylene production; genes; hydrogen peroxide; ions; phospholipase D; receptors; seedlings; signal transduction
Abstract:
... In higher plants, copper ions, hydrogen peroxide, and cycloheximide have been recognized as very effective inducers of the transcriptional activity of genes encoding the enzymes of the ethylene biosynthesis pathway. In this report, the transcriptional patterns of genes encoding the 1-aminocyclopropane-1-carboxylate synthases (ACSs), 1-aminocyclopropane-1-carboxylate oxidases (ACOs), ETR1, ETR2, an ...
DOI:
10.1093/jxb/erq177
PubMed:
20581125
PubMed Central:
PMC2905205

21. Fungal and bacterial mediated denitrification in wetlands: Influence of sediment redox condition

Author:
Seo, Dong Cheol; DeLune, R.D.
Source:
Water research 2010 v.44 no.8 pp. 2441-2450
ISSN:
0043-1354
Subject:
sediments; redox potential; aerobic conditions; cycloheximide; nitrous oxide; streptomycin; swamps; soil respiration; denitrification; oxidation; gas emissions; lowland forests; microbial activity; bacteria; fungi; Louisiana
DOI:
10.1016/j.watres.2010.01.006
PubMed:
20122708

22. Identification and characterization of dehydrins in horse chestnut recalcitrant seeds

Author:
Gumilevskaya, N. A.; Azarkovich, M. I.
Source:
Russian journal of plant physiology 2010 v.57 no.6 pp. 859-864
ISSN:
1021-4437
Subject:
Aesculus hippocastanum; abscisic acid; alpha-amanitin; antibodies; cotyledons; cycloheximide; dormancy; germination; glucose; heat stability; immunoblotting; petioles; polypeptides; proteasome endopeptidase complex; recalcitrant seeds; tissues; ubiquitin; ubiquitination
Abstract:
... The fraction of heat-stable dehydrins cytosolic proteins from mature recalcitrant seeds of horse chestnut (Aesculus hippocastanum L.) was studied in the period of their dormancy and germination in order to identify and characterize stress-induced dehydrin-like polypeptides. In our experiments, in tissues of dormant seeds, dehydrin was identifies by immunoblotting as a single bright band with a mol ...
DOI:
10.1134/S1021443710060154

23. In Vitro Ageing of Porcine Oocytes: Changes in Phosphorylation of the Mitogen‐Activated Protein Kinase (MAPK) and Parthenogenetic Activability

Author:
Ebeling, S.; Labudda, A.; Meinecke, B.
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. e398
ISSN:
0936-6768
Subject:
anaphase; cycloheximide; dephosphorylation; ethanol; metaphase; mitogen-activated protein kinase; oocytes; parthenogenesis; phosphorylation; swine
Abstract:
... The role of mitogen‐activated protein kinase (MAPK) was investigated during ageing of porcine oocytes following in vitro maturation (IVM). Oocytes exhibiting an extruded first polar body after IVM for 46 h (79.3% metaphase II, M II) were used for the experiments. Nuclear maturation stages were not visibly altered after a further 12 h of ageing. Proportion of M II stages (42.9%) decreased significa ...
DOI:
10.1111/j.1439-0531.2010.01588.x
PubMed:
20210885

24. Influence of indigenous eukaryotic microbial communities on the reduction of Escherichia coli O157:H7 in compost slurry

Author:
Puri, Amrita; Dudley, Edward G.
Source:
FEMS microbiology letters 2010 v.313 no.2 pp. 148-154
ISSN:
0378-1097
Subject:
DNA libraries; Escherichia coli O157; animal manures; composts; cycloheximide; denaturing gradient gel electrophoresis; fresh produce; fungal communities; predation; sequence analysis; slurries; species diversity
Abstract:
... Compost made from livestock manure is commonly used as a crop fertilizer and serves as a possible vehicle for the transmission of Escherichia coli O157:H7 to fresh produce. In this study, we hypothesized that the indigenous microbial communities present in composts adversely affects the survival of E. coli O157:H7. Escherichia coli O157:H7 was spiked into compost slurry and incubated at 25 °C. Esc ...
DOI:
10.1111/j.1574-6968.2010.02141.x

25. Meningococcal Porin PorB Prevents Cellular Apoptosis in a Toll-Like Receptor 2- and NF-κB-Independent Manner

Author:
Massari, Paola; Gunawardana, Jay; Liu, Xiuping; Wetzler, Lee M.
Source:
Infection and immunity 2010 v.78 no.3 pp. 994-1003
ISSN:
0019-9567
Subject:
apoptosis; cycloheximide; porins; signal transduction; tumor necrosis factor-alpha
Abstract:
... Meningococcal porin PorB is an inhibitor of apoptosis induced via the intrinsic pathway in various cell types. This effect is attributed to prevention of mitochondrial depolarization and of subsequent release of proapoptotic mitochondrial factors. To determine whether apoptosis is globally inhibited by PorB, we compared the intrinsic and extrinsic pathways in HeLa cells. Interestingly, PorB does n ...
DOI:
10.1128/IAI.00156-09
PubMed:
20028813
PubMed Central:
PMC2825956

26. Midgut and fatbody mitochondrial transhydrogenase activities during larval-pupal development of the tobacco hornworm, Manduca sexta

Author:
Vandock, Kurt P.; Drummond, Christopher A.; Smith, Stan L.; Fioravanti, Carmen F.
Source:
Journal of insect physiology 2010 v.56 no.7 pp. 774-779
ISSN:
0022-1910
Subject:
Manduca sexta; larvae; pupae; larval development; pupal development; biochemical pathways; midgut; fat body; mitochondria; hydrogenation; NADP (coenzyme); NAD (coenzyme); enzyme activity; biosynthesis; ecdysone; ecdysterone; cycloheximide; biotransformation
Abstract:
... Midgut and fatbody mitochondria from fifth larval instar Manduca sexta display a membrane-associated transhydrogenase that catalyzes a reversible hydride ion transfer between NADP(H) and NAD(H). The NADPH-forming activity occurs as a nonenergy- or energy-linked activity with energy for the latter derived from either electron transport-dependent NADH or succinate utilization, or ATP hydrolysis by M ...
DOI:
10.1016/j.jinsphys.2010.01.008
PubMed:
20138054

27. Mycovirus in Pseudocercospora griseola, the causal agent of angular leaf spot in common bean

Author:
Lima, Swiany Silveira; Abadio, Ana Karina Rodrigues; Araújo, Elza Fernandes; Kitajima, Elliot Watanabe; Sartorato, Aloísio; Queiroz, Marisa Vieira de
Source:
Canadian journal of microbiology 2010 v.56 no.5 pp. 359-365
ISSN:
0008-4166
Subject:
Phaseolus vulgaris; Pseudocercospora; agarose; beans; conidia; cycloheximide; deoxyribonucleases; double-stranded RNA; gel electrophoresis; leaf spot; mycelium; mycoviruses; plant pathogenic fungi; ribonucleases; transmission electron microscopy
Abstract:
... Pseudocercospora griseola (Sacc.) Crous & Braun is a widespread fungal phytopathogen that is responsible for angular leaf spot in the common bean (Phaseolus vulgaris L.). A number of fungal phytopathogens have been shown to harbour mycoviruses, and this possibility was investigated in populations of Pseudocercospora griseola. The total nucleic acid extracts of 61 fungal isolates were subjected to ...
DOI:
10.1139/W10-022

28. A Novel Material Incorporation Technique for Medaka (Oryzias latipes) Eggs Using Nanosecond Pulsed Electric Fields

Author:
Nobuaki TOMINAGA; Susumu KONO; Akemi YAMAGUCHI; Hidenori AKIYAMA; Koji ARIZONO
Source:
Bioscience, biotechnology, and biochemistry 2010 v.74 no.6 pp. 1279-1282
ISSN:
1347-6947
Subject:
Oryzias latipes; biochemistry; biotechnology; cell membranes; chorion; cycloheximide; eggs; embryogenesis; permeability
Abstract:
... The effects of nanosecond pulsed electric fields (nsPEFs) on medaka eggs were examined. Although embryogenesis was not affected by nsPEF treatment alone, significant harmful effects were observed when the eggs were treated with nsPEFs in the presence of cycloheximide in the outer solution. Nanosecond PEF treatment affected the permeability of both the egg envelope (chorion) and the cell membrane, ...
DOI:
10.1271/bbb.90958

29. Parthenogenetic development of domestic cat oocytes treated with ionomycin, cycloheximide, roscovitine and strontium

Author:
Rascado, T. da Silva; Martins, L. Rigatto; Minto, B. Watanabe; de Sá Lorena, S.E.R.; Landim-Alvarenga, F. da Cruz
Source:
Theriogenology 2010 v.74 no.4 pp. 596-601
ISSN:
0093-691X
Subject:
cats; oocytes; parthenogenesis; cultured cells; culture media; cumulus oophorus; cycloheximide; strontium; embryogenesis; morula; dosage; dose response; calcium; cations
Abstract:
... The objective was to evaluate the parthenogenetic activation of domestic cat oocytes. Cumulus-oocyte complexes matured for 36 h were subjected to three protocols of parthenogenetic activation: Group 1 - ionomycin + cycloheximide; Group 2 - ionomycin + roscovitine; and Group 3 - ionomycin + strontium. As a control, a fourth group of oocytes were cultured in the absence of any activation agent. In a ...
DOI:
10.1016/j.theriogenology.2010.03.010
PubMed:
20452001

30. Protein synthesis is differentially required for germination in Poa pratensis and Trifolium repens in the absence or in the presence of cadmium

Author:
de Lespinay, Alexis; Lequeux, Hélène; Lambillotte, Béatrice; Lutts, Stanley
Source:
Plant growth regulation 2010 v.61 no.2 pp. 205-214
ISSN:
0167-6903
Subject:
Poa pratensis; Trifolium repens; actinomycin D; alpha-amanitin; beta-amylase; cadmium; cycloheximide; gene expression; genes; messenger RNA; osmotic treatment; protein synthesis; proteins; seed germination; seed priming; seeds
Abstract:
... The objective of this study was to determine the importance of protein synthesis during germination of white clover (Trifolium repens) and Kentucky bluegrass (Poa pratensis) and to assess the impact of osmopriming on seed germination in the presence of cadmium. Cycloheximide (Cx, 20 and 100 μM), a translation inhibitor, delayed germination and reduced final germination percentage in both species, ...
DOI:
10.1007/s10725-010-9471-z

31. Regulation of pseudorabies virus gG glycoprotein gene promoter independently of pseudorabies immediate early IE180 protein

Author:
Muñoz, A. L.; Torres, M.; Martín, B.; Lerma, L.; Tabarés, E.
Source:
Archives of virology 2010 v.155 no.4 pp. 515-523
ISSN:
0304-8608
Subject:
Suid alphaherpesvirus 1; messenger RNA; transgenes; Aujeszky disease; plasmids; transfection; viruses; glycoproteins; cycloheximide
Abstract:
... The pseudorabies virus (PRV) glycoprotein known as gG is generally regarded as an early protein, and the immediate early IE180 protein regulates its expression during infection. This study, however, provides evidence that although induction by IE180 is observed, the expression of a marker protein (EGFP), or gG itself, under the control of the gG promoter, can also occur independently of the expres ...
DOI:
10.1007/s00705-010-0613-4
PubMed:
20186446

32. Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP

Author:
Kurata, Shinya; Nielsen, Klaus H.; Mitchell, Sarah F.; Lorsch, Jon R.; Kaji, Akira; Kaji, Hideko
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.24 pp. 10854-10859
ISSN:
0027-8424
Subject:
Saccharomyces cerevisiae; adenosine; adenosine triphosphate; aminoglycosides; cycloheximide; dissociation; guanosine triphosphate; messenger RNA; models; protein synthesis; ribosomes; transfer RNA; translation (genetics); yeasts
Abstract:
... After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5γ'-(β,γ-imido)triphosphate ...
DOI:
10.1073/pnas.1006247107
PubMed:
20534490
PubMed Central:
PMC2890720

33. Role of Light in the Appearance of Glutamine Synthetase in Leaves of Pennisetum glaucum

Author:
Shilpi Ghosh
Source:
Journal of plant biochemistry and biotechnology 2010 v.19 no.2 pp. 239-242
ISSN:
0971-7811
Subject:
Pennisetum glaucum; cycloheximide; etiolation; glutamate-ammonia ligase; isozymes; leaves; plant age; seedlings; sucrose; urea
Abstract:
... Leaves of Pennisetum [Pennisetum glaucum (L) HHB 67] seedlings contained two isozymes of glutamine synthetase (GS, EC 6.3.1.2): cytosolic GS1 and chloroplastic GS2. Leaves of seedlings grown in light for seven days contained about twofold higher GS activity than etiolated leaves. In both light and dark grown seedlings, total GS, GS1 and GS2 activity declined with plant age with more pronounced eff ...
DOI:
10.1007/BF03263347

34. Site-directed mutagenesis of Asp853 in Pdr3p transcriptional activator from Saccharomyces cerevisiae

Author:
Dzugasova, Vladimira; Borecka, Silvia; Batova, Monika; Pilisiova, Ruzena; Hervayova, Nora; Subik, Julius
Source:
Yeast 2010 v.27 no.5 pp. 277-284
ISSN:
0749-503X
Subject:
transcription factors; transcriptional activation; alleles; multiple drug resistance; yeasts; Saccharomyces cerevisiae; transcription (genetics); amino acids; mutants; site-directed mutagenesis; adverse effects; cycloheximide
Abstract:
... The PDR3 gene encodes one of the main transcriptional activators involved in the control of multidrug resistance in the yeast Saccharomyces cerevisiae. Recently, it has been demonstrated that a specific D853Y mutation results in the loss of transactivation activity of Pdr3p and its conversion to multicopy suppressor of multidrug resistance. In this study, the Asp853 in Pdr3p was replaced by eight ...
DOI:
10.1002/yea.1751
PubMed:
20146400

35. Storage proteins and cell wall mobilisation in seeds of Sesbania virgata (Cav.) Pers. (Leguminosae)

Author:
Tonini, Patricia P.; Carrara, Thalita B.; Buckeridge, Marcos S.
Source:
Trees 2010 v.24 no.4 pp. 675-684
ISSN:
0931-1890
Subject:
seedlings; Sesbania; trees; carbon; polypeptides; cell walls; structural proteins; storage proteins; transcription factors; actinomycin D; proteolysis; alpha-galactosidase; enzyme activity; seed germination; protein bodies; translation (genetics); nitrogen; galactomannans; proteinases; alpha-amanitin; endosperm; protein degradation; cycloheximide
Abstract:
... The endosperm of seeds of Sesbania virgata (Cav.) Pers. accumulates galactomannan as a cell wall storage polysaccharide. It is hydrolysed by three enzymes, one of them being α-galactosidase. A great amount of protein bodies is found in the cytoplasm of endospermic cells, which are thought to play the major role as a nitrogen reserve in this seed. The present work aimed at understanding how the pro ...
DOI:
10.1007/s00468-010-0437-6

36. Stored and neosynthesized mRNA in Arabidopsis seeds: effects of cycloheximide and controlled deterioration treatment on the resumption of transcription during imbibition

Author:
Kimura, Mitsuhiro; Nambara, Eiji
Source:
Plant molecular biology 2010 v.73 no.1-2 pp. 119-129
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; imbibition; messenger RNA; protein synthesis; seed dormancy; seed germination; seeds; transcription factors; microarray technology; translation (genetics); transcription (genetics); gene expression; genes; physiological response; transcriptome; seed maturation; cycloheximide
Abstract:
... Dry seeds accumulate translatable mRNAs as well as functional proteins for transcription and translation. They are possibly involved in early physiological responses after imbibition, however, their functions remain poorly understood. The aim of this study is to investigate the function of seed stored transcriptional machinery in resumption of gene expression after the onset of imbibition in Arabi ...
DOI:
10.1007/s11103-010-9603-x
PubMed:
20099072

37. Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells

Author:
Heller, S.R.; Rodrigues Goulart, H.; Arthuso, F.S.; Oliveira, T.L.; Bartolini, P.; Soares, C.R.J.
Source:
Journal of biotechnology 2010 v.145 no.4 pp. 334-340
ISSN:
0168-1656
Subject:
Western blotting; bioactive properties; bioassays; biotechnology; cycloheximide; eukaryotic cells; glycosylation; high performance liquid chromatography; humans; lactation; mammary development; mammary glands; matrix-assisted laser desorption-ionization mass spectrometry; prolactin
Abstract:
... Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which is partially (10-30%) occupied when the protein is synthesized in eukaryotic cells. Although the biological activity of glyc ...
DOI:
10.1016/j.jbiotec.2009.12.019

38. TIMP-1 interaction with αvβ3 integrin confers resistance to human osteosarcoma cell line MG-63 against TNF-α-induced apoptosis

Author:
Tsagaraki, Ioanna; Tsilibary, Effie C.; Tzinia, Athina K.
Source:
Cell and tissue research 2010 v.342 no.1 pp. 87-96
ISSN:
0302-766X
Subject:
antagonists; apoptosis; cell viability; confocal microscopy; cycloheximide; cytotoxicity; extracellular matrix; human cell lines; integrins; neoplasm cells; osteosarcoma; precipitin tests; small interfering RNA; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine affecting diverse cellular responses. TNF-α is cytotoxic in many systems, but it can also act as an anti-apoptotic signal to promote cell survival pathways activated through integrins and extracellular matrix components. This is particularly evident in cancer cells. To unravel the basis of resistance to TNF-α-induced apoptosis, human osteos ...
DOI:
10.1007/s00441-010-1025-1

39. Toll-Like Receptor 2- and MyD88-Dependent Phosphatidylinositol 3-Kinase and Rac1 Activation Facilitates the Phagocytosis of Listeria monocytogenes by Murine Macrophages

Author:
Shen, Yanna; Kawamura, Ikuo; Nomura, Takamasa; Tsuchiya, Kohsuke; Hara, Hideki; Dewamitta, Sita R.; Sakai, Shunsuke; Qu, Huixin; Daim, Sylvia; Yamamoto, Takeshi; Mitsuyama, Masao
Source:
Infection and immunity 2010 v.78 no.6 pp. 2857-2867
ISSN:
0019-9567
Subject:
Gram-positive bacteria; Listeria monocytogenes; cycloheximide; cytokines; guanosinetriphosphatase; intravenous injection; lipopolysaccharides; macrophages; mice; models; phagocytosis; phosphatidylinositol 3-kinase; receptors
Abstract:
... Toll-like receptors (TLRs) play a key role in the innate immune response by sensing bacterial ligands. The mechanisms involved in the TLR-mediated cytokine response are well established; however, the possible contribution of TLR-dependent recognition of bacteria to macrophage phagocytosis remains unclear. Listeria monocytogenes is an intracellular, parasitic, Gram-positive bacterium recognized mai ...
DOI:
10.1128/IAI.01138-09
PubMed:
20368346
PubMed Central:
PMC2876581

40. Toxicity of fungicides to natural bacterial communities in wetland water and sediment measured using leucine incorporation and potential denitrification

Author:
Milenkovski, Susann; Bååth, Erland; Lindgren, Per-Eric; Berglund, Olof
Source:
Ecotoxicology 2010 v.19 no.2 pp. 285-294
ISSN:
0963-9292
Subject:
antibiotics; aquatic environment; bacterial communities; benomyl; captan; carbendazim; carboxin; cycloheximide; denitrification; fenpropimorph; leucine; propiconazole; sediments; thiram; toxicity
Abstract:
... We assessed potential toxicity of fungicides to natural bacterial communities from a constructed wetland, located in southern Sweden, and compared the sensitivity of two endpoints indicating bacterial activity, leucine incorporation, and potential denitrification, in detecting toxicity. The effects of eight fungicides (benomyl, carbendazim, carboxin, captan, cycloheximide, fenpropimorph, propicona ...
DOI:
10.1007/s10646-009-0411-5
PubMed:
19768538

41. Transpiration response of 'slow-wilting' and commercial soybean (Glycine max (L.) Merr.) genotypes to three aquaporin inhibitors

Author:
Sadok, Walid; Sinclair, Thomas R.
Source:
Journal of experimental botany 2010 v.61 no.3 pp. 821-829
ISSN:
0022-0957
Subject:
Glycine max; cycloheximide; drought tolerance; genotype; guard cells; leaf conductance; leaves; soil conservation; soybeans; symplast; vapor pressure; water flow
Abstract:
... The slow-wilting soybean [Glycine max (L.) Merr.] genotype, PI 416937, exhibits a limiting leaf hydraulic conductance for transpiration rate (TR) under high vapour pressure deficit (VPD). This genotype has a constant TR at VPD greater than 2 kPa, which may be responsible for its drought tolerance as a result of soil water conservation. However, the exact source of the hydraulic limitation between ...
DOI:
10.1093/jxb/erp350
PubMed:
19969533
PubMed Central:
PMC2814113

42. The Trypanosoma cruzi nucleolus: a morphometrical analysis of cultured epimastigotes in the exponential and stationary phases

Author:
Nepomuceno‐Mejía, Tomás; Lara‐Martínez, Reyna; Cevallos, Ana María; López‐Villaseñor, Imelda; Jiménez‐García, Luis Felipe; Hernández, Roberto
Source:
FEMS microbiology letters 2010 v.313 no.1 pp. 41-46
ISSN:
0378-1097
Subject:
Trypanosoma cruzi; biogenesis; cell nucleolus; cultured cells; cycloheximide; epimastigotes; models; morphometry; ribosomal RNA; ribosomes
Abstract:
... Our group is interested in rRNA and ribosome biogenesis in the parasitic protozoan Trypanosoma cruzi. Epimastigotes represent an extracellular replicative stage of T. cruzi and can be cultured in axenic media. The growth curve of epimastigotes allows assessment of potential differences in the nucleoli of cells undergoing growth‐rate transitions. To establish cellular parameters for studying riboso ...
DOI:
10.1111/j.1574-6968.2010.02117.x

43. Vmr 1p is a novel vacuolar multidrug resistance ABC transporter in Saccharomyces cerevisiae

Author:
Wawrzycka, Donata; Sobczak, Iwona; Bartosz, Grzegorz; Bocer, Tomasz; Ułaszewski, Stanisław; Goffeau, André
Source:
FEMS yeast research 2010 v.10 no.7 pp. 828-838
ISSN:
1567-1356
Subject:
2,4-D; 2,4-dinitrophenol; ABC transporters; Saccharomyces cerevisiae; cadmium; cycloheximide; drugs; ethanol; genes; glucose; glycerol; multiple drug resistance; toxicity; vacuoles; yeasts
Abstract:
... The Saccharomyces cerevisiae Yhl035p/Vmr1p is an ABC transporter of the MRP subfamily that is conserved in all post Whole Genome Duplication species. The deletion of the YHL035 gene caused growth sensitivity to several amphiphilic drugs such as cycloheximide, 2,4-dichlorophenoxyacetic acid, 2,4-dinitrophenol as well as to cadmium and other toxic metals. Vmr1p-GFP was located in the vacuolar membra ...
DOI:
10.1111/j.1567-1364.2010.00673.x
PubMed:
20846144

44. Xenopus furry contributes to release of microRNA gene silencing

Author:
Goto, Toshiyasu; Fukui, Akimasa; Shibuya, Hiroshi; Keller, Ray; Asashima, Makoto
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.45 pp. 19344-19349
ISSN:
0027-8424
Subject:
Xenopus; cycloheximide; gene expression; gene silencing; genes; herpes simplex; leucine zipper; microRNA; oligonucleotides; protein synthesis; transcription (genetics); vertebrates; viruses
Abstract:
... A transcriptional corepressor, Xenopus furry (Xfurry), is expressed in the chordamesodermal region and induces secondary dorsal axes when overexpressed on the ventral side of the embryo. The N-terminal furry domain functions as a repressor, and the C-terminal leucine zipper (LZ) motifs /coiled-coil structure, found only in vertebrate homologs, contributes to the nuclear localization. The engrailed ...
DOI:
10.1073/pnas.1008954107
PubMed:
20974966
PubMed Central:
PMC2984173

45. The serine/threonine protein phosphatase Sit4p activates multidrug resistance in Saccharomyces cerevisiae

Author:
Miranda, Michel N.; Masuda, Claudio A.; Ferreira-Pereira, Antonio; Carvajal, Elvira; Ghislain, Michel; Montero-Lomelí, Mónica
Source:
FEMS yeast research 2010 v.10 no.6 pp. 674-686
ISSN:
1567-1356
Subject:
ABC transporters; Candida albicans; Saccharomyces cerevisiae; alleles; azoles; cycloheximide; daunorubicin; gain-of-function mutation; multiple drug resistance; mutants; regulatory proteins; serine; threonine; transcription (genetics); transcription factors; yeasts; zinc
Abstract:
... Multidrug resistance in Saccharomyces cerevisiae is frequently associated with gain-of-function mutations in zinc finger-containing transcription factors Pdr1p and Pdr3p. These regulatory proteins activate the expression of several ATP-binding cassette transporter genes, leading to elevated drug resistance. Here, we report that loss of the type 2A-related serine/threonine protein phosphatase Sit4p ...
DOI:
10.1111/j.1567-1364.2010.00656.x
PubMed:
20608983

46. A- type lamin dynamics in bovine somatic cell nuclear transfer embryos

Author:
Kelly, Richard D.W.; Alberio, Ramiro; Campbell, Keith H.S.
Source:
Reproduction, fertility, and development 2010 v.22 no.6 pp. 956-965
ISSN:
1031-3613
Subject:
blastocyst; cattle; cycloheximide; in vitro fertilization; monoclonal antibodies; parthenogenesis; pronucleus; protein synthesis; somatic cells; staining
Abstract:
... The persistence of A-type nuclear lamin in somatic cell nuclear transfer (SCNT) embryos has been proposed as a marker for incomplete nuclear reprogramming. Using monoclonal antibodies to A/C- (A/C-346 and A/C-131C3) and B-type lamin, we compared distribution during early development of bovine IVF, parthenogenetic and SCNT embryos. A/C-346 staining was observed in the pronuclei of IVF embryos and i ...
DOI:
10.1071/RD09264
PubMed:
20591330

47. The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch

Author:
Hellman, Nathan E.; Liu, Yan; Merkel, Erin; Austin, Christina; Le Corre, Stephanie; Beier, David R.; Sun, Zhaoxia; Sharma, Neeraj; Yoder, Bradley K.; Drummond, Iain A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.43 pp. 18499-18504
ISSN:
0027-8424
Subject:
Danio rerio; animal injuries; cilia; cycloheximide; epithelium; gene expression regulation; genes; gentamicin; homeostasis; kidney diseases; kidneys; mice; transcription (genetics); transcription factors
Abstract:
... Cilia are essential for normal organ function and developmental patterning, but their role in injury and regeneration responses is unknown. To probe the role of cilia in injury, we analyzed the function of foxj1, a transcriptional regulator of cilia genes, in response to tissue damage and renal cyst formation. Zebrafish foxj1a, but not foxj1b, was rapidly induced in response to epithelial distensi ...
DOI:
10.1073/pnas.1005998107
PubMed:
20937855
PubMed Central:
PMC2972951