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1. Characterization of monoclonal antibodies against goat interleukin-18 and their application in the measurement of goat interleukin-18 in LPS-stimulated peripheral blood mononuclear cells by sandwich ELISA

Author:
Cao, Bing-lei; Qiu, Hong-kai; Hu, Jing-dong; Meng, Fan-dong; Zhang, Hong; Zhao, Hong-kun
Source:
Veterinary immunology and immunopathology 2010 v.138 no.3 pp. 235-238
ISSN:
0165-2427
Subject:
protein binding; hybridomas; lipopolysaccharides; quantitative analysis; interleukins; monocytes; antigens; goats; inflammation; Western blotting; monoclonal antibodies; enzyme-linked immunosorbent assay; immune response; measurement; fluorescent antibody technique
Abstract:
... In order to develop a specific assay for the measurement of goat IL-18 level, two stable hybridoma cell lines were established which secreted IgG1 monoclonal antibodies (mAbs) against goat IL-18. Specific binding of two mAbs named 2E8 and 4C4 to recombinant goat IL-18 expressed in Escherichia coli was demonstrated in an ELISA and Western blotting. Results also showed that mAbs 2E8 and 4C4 bound to ...
DOI:
10.1016/j.vetimm.2010.07.005

2. Effect of Panax ginseng on cytokine expression in bovine mammary glands at drying off

Author:
Baravalle, C.; Dallard, B.E.; Ortega, H.H.; Neder, V.E.; Canavesio, V.R.; Calvinho, L.F.
Source:
Veterinary immunology and immunopathology 2010 v.138 no.3 pp. 224-230
ISSN:
0165-2427
Subject:
dairy cows; temporal variation; messenger RNA; interleukin-1; immunomodulators; lactation; herbal medicines; biochemical pathways; Panax ginseng; reverse transcriptase polymerase chain reaction; Western blotting; gene expression; tumor necrosis factor-alpha; mammary glands; medicinal properties; somatic cell count; udders
Abstract:
... Biological response modifiers (BRM) are agents that modify the host's response to pathogens with resultant beneficial prophylactic or therapeutic effects. The objectives of this study were to describe the immunomodulatory effects of Panax ginseng (GS) on bovine mammary glands at the end of lactation. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10mL of BRM ( ...
DOI:
10.1016/j.vetimm.2010.07.019

3. Wide Distribution among Halophilic Archaea of a Novel Polyhydroxyalkanoate Synthase Subtype with Homology to Bacterial Type III Synthases

Author:
Han, Jing; Hou, Jing; Liu, Hailong; Cai, Shuangfeng; Feng, Bo; Zhou, Jian; Xiang, Hua
Source:
Applied and environmental microbiology 2010 v.76 no.23 pp. 7811-7819
ISSN:
0099-2240
Subject:
DNA primers; Haloarcula; Western blotting; amino acid sequences; amino acids; antiserum; bacteria; biosynthesis; carbon sequestration; enzyme activity; fermentation; genes; molecular weight; phylogeny; polyhydroxyalkanoates; polymerase chain reaction
Abstract:
... Polyhydroxyalkanoates (PHAs) are accumulated as intracellular carbon and energy storage polymers by various bacteria and a few haloarchaea. In this study, 28 strains belonging to 15 genera in the family Halobacteriaceae were investigated with respect to their ability to synthesize PHAs and the types of their PHA synthases. Fermentation results showed that 18 strains from 12 genera could synthesize ...
DOI:
10.1128/AEM.01117-10
PubMed:
20889776
PubMed Central:
PMC2988587

4. Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

Author:
Rennie, Bryan; Filion, Lionel G; Smart, Nonie
Source:
BMC veterinary research 2010 v.6 no.1 pp. 230
ISSN:
1746-6148
Subject:
Mycobacterium bovis; Western blotting; antibodies; antibody detection; bovine tuberculosis; cattle; proteins; skin tests; tuberculin; two-dimensional gel electrophoresis
Abstract:
... BACKGROUND: Bovine tuberculosis, caused by Mycobacterium bovis, afflicts approximately 50 million cattle worldwide and is detected by the tuberculin skin test (TST). While it has long been recognized that purified protein derivative (PPD) tuberculin is composed of a mixture of M. bovis derived protein components, little is known about the quality, relative quantity and identity of the proteins tha ...
DOI:
10.1186/1746-6148-6-50
PubMed:
21062483
PubMed Central:
PMC2994848

5. A Study to Demonstrate Freedom from Trichinella spp. in Domestic Pigs in Switzerland

Author:
Schuppers, M.E.; Rosenberg, G.; Graf, R.; Eidam, V.; Wittwer, C.; Zimmermann, W.; Gottstein, B.; Frey, C.F.
Source:
Zoonoses and public health 2010 v.57 no.7-8 pp. e130
ISSN:
1863-1959
Subject:
swine; swine diseases; Trichinella; trichinosis; disease surveillance; enzyme-linked immunosorbent assay; Western blotting; disease prevalence; disease detection; diagnostic techniques; Switzerland
Abstract:
... Trichinellosis is a food‐borne zoonotic disease caused by the nematode Trichinella spp. Many omnivorous and carnivorous animal species can act as host for this parasite, including domestic pigs. To protect public health, it should be ensured that pork should not contain infective Trichinella larvae. Surveillance for Trichinella spp. can be done using direct (larval detection) and indirect (antibod ...
DOI:
10.1111/j.1863-2378.2009.01299.x

6. Berberine inhibits SREBP‐1‐related clozapine and risperidone induced adipogenesis in 3T3‐L1 cells

Author:
Hu, Yueshan; Kutscher, Eric; Davies, Gareth E.
Source:
Phytotherapy research 2010 v.24 no.12 pp. 1831-1838
ISSN:
0951-418X
Subject:
Western blotting; adiponectin; animal models; berberine; cell differentiation; cell viability; cytotoxicity; gene expression; lipogenesis; messenger RNA; protein synthesis; proteins; weight gain
Abstract:
... Weight gain is a common and potentially serious complication associated with the treatment of second generation antipsychotics such as clozapine and risperidone. Increased peripheral adipogenesis via the SREBP‐1 pathway could be one critical mechanism responsible for antipsychotic drug‐induced weight gain. Berberine, a botanic alkaloid, has been shown in our previous studies to inhibit adipogenesi ...
DOI:
10.1002/ptr.3204
PubMed:
20564506

7. Butyrate increases the formation of anti-angiogenic vascular endothelial growth factor variants in human lung microvascular endothelial cells

Author:
Ciura, J.; Jagodziński, P. P.
Source:
Molecular biology reports 2010 v.37 no.8 pp. 3729-3734
ISSN:
0301-4851
Subject:
RNA; Western blotting; alternative splicing; angiogenesis; antineoplastic agents; cultured cells; endothelial cells; humans; neoplasms; quantitative polymerase chain reaction; reverse transcription; sodium butyrate; vascular endothelial growth factors
Abstract:
... The primary transcript of vascular endothelial growth factor (VEGF) can be alternatively spliced and translated to pro-angiogenic and anti-angiogenic VEGF variants. We investigated the effect of sodium butyrate (NaB) on pro-angiogenic and anti-angiogenic VEGF variants production in immortalized human lung microvascular endothelial cells (HLMEC). These cells were cultured in the absence or in the p ...
DOI:
10.1007/s11033-010-0026-1

8. Changes of Connexin43 Expression in Non‐pregnant Porcine Myometrium Correlate with Progesterone Concentration During Oestrous Cycle

Author:
Karasinski, J.; Galas, J.; Semik, D.; Fiertak, A.; Bilinska, B.; Kilarski, WM
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. 959-966
ISSN:
0936-6768
Subject:
Western blotting; estrous cycle; gap junctions; myometrium; progesterone; steroid hormones; swine; uterine contraction
Abstract:
... Connexin43 (Cx43) is a major protein of myometrial gap junctions. The number of Cx43 gap junctions increase dramatically with the onset of labour in association with development of synchronized uterine contractions. The formation of myometrial gap junctions follows an increase in the oestrogen to progesterone ratio indicating an important role of steroid hormones in regulating Cx43 expression at t ...
DOI:
10.1111/j.1439-0531.2009.01468.x
PubMed:
20149142

9. Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

Author:
Mo, Xiao-han; Chen, Zheng-bin; Chen, Jian-ping
Source:
Virus genes 2010 v.41 no.3 pp. 425-431
ISSN:
0920-8569
Subject:
Aphidoidea; Nicotiana tabacum; Polerovirus; RNA; Tobacco bushy top virus; Western blotting; antiserum; capsid; coat proteins; genome; nucleotides; open reading frames; phylogeny; polyacrylamide gel electrophoresis; reverse transcriptase polymerase chain reaction; sequence analysis; tobacco; virion; viruses
Abstract:
... Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. The complete ge ...
DOI:
10.1007/s11262-010-0524-1

10. Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Author:
Yeng, Chen; Osman, Emelia; Mohamed, Zeehaida; Noordin, Rahmah
Source:
Electrophoresis 2010 v.31 no.23-24 pp. 3843-3849
ISSN:
0173-0835
Subject:
Toxoplasma gondii; Western blotting; active sites; blood proteins; blood serum; circulating antigens; electrophoresis; gels; glycoproteins; host specificity; immunocompromised population; mass spectrometry; parasites; pregnant women; toxoplasmosis
Abstract:
... Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life‐threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2‐DE and Western blot methods were employed to study the parasite circulating antigens and host‐specific proteins in the sera of T. gondii‐infected individuals. The comparisons were made b ...
DOI:
10.1002/elps.201000038

11. Development of a Competitive ELISA for the Detection of Pecan (Carya illinoinensis (Wangenh.) K. Koch) Traces in Food

Author:
Polenta, Gustavo; Godefroy-Benrejeb, Samuel; Delahaut, Philippe; Weber, Dorcas; Abbott, Michael
Source:
Food analytical methods 2010 v.3 no.4 pp. 375-381
ISSN:
1936-9751
Subject:
Brazil nuts; Carya illinoinensis; Western blotting; allergens; enzyme-linked immunosorbent assay; food allergies; food industry; food matrix; foods; hazelnuts; ingredients; inhibitory concentration 50; pecans; polyacrylamide gel electrophoresis; polyclonal antibodies; proteins; rabbits; sodium dodecyl sulfate
Abstract:
... Pecan nuts are included among the so-called big-eight food allergens, which are responsible for 90% of food allergies. However, unlike other tree nuts such as Brazil nut and hazelnut, studies on pecan from the point of view of allergies are scarce, and only a few analytical methods to detect its presence in food have been published. The objective of the present study was to develop an enzyme-linke ...
DOI:
10.1007/s12161-009-9075-2

12. Early Meiotic‐Specific Protein Expression in Post‐natal Rat Ovaries

Author:
Zhang, P.; Lv, LX; Xing, WJ
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. e447
ISSN:
0936-6768
Subject:
Western blotting; animal ovaries; cortex; epithelium; females; genes; immunohistochemistry; mice; mitosis; protein synthesis; proteins; rats; retinoic acid; stem cells; synaptonemal complex; translation (genetics)
Abstract:
... Recent studies in mice challenged the basic doctrine that most mammalian females lose neo‐oogenesis in post‐natal ovaries. In order to provide more information in other species, we examined post‐natal rat ovaries by histological sections and detected the germline cell marker protein RVLG (rat vasa‐like gene), BrdU (5‐bromodeoxyuridine) incorporation in RVLG‐expressing cells, for identification of ...
DOI:
10.1111/j.1439-0531.2010.01599.x
PubMed:
20345585

13. Effect of Cyclin‐dependent Kinase (CDK) Inhibition on Expression, Localization and Activity of Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase (MAPK) in Bovine Oocytes

Author:
Quetglas, MD; Adona, PR; De Bem, THC; Pires, PRL; Leal, CLV
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. 1074-1081
ISSN:
0936-6768
Subject:
Western blotting; cattle; cytoplasm; fluorescent antibody technique; germinal vesicle; maturation-promoting factor; meiosis; messenger RNA; mitogen-activated protein kinase; polymerase chain reaction; proteins
Abstract:
... This study aimed to assess the effects of cyclin‐dependent kinase (CDK) inhibition on factors involved in the control of meiosis in bovine oocytes: maturation promoting factor (MPF) (p34cdc² and cyclin B1) and mitogen activated protein kinase (MAPK). Oocytes were maintained at germinal vesicle (GV) stage in vitro with 10 μm of the CDK inhibitor butyrolactone I (BLI) for 24 h (inhibited). After thi ...
DOI:
10.1111/j.1439-0531.2009.01498.x
PubMed:
19602178

14. Effects of ionophores on liver CYP1A and 3A in male broilers

Author:
ZHANG, L.L.; ZHANG, J.R.; YU, Z.G.; ZHAO, J.; MO, F.; JIANG, S.X.
Source:
Journal of veterinary pharmacology and therapeutics 2010 v.33 no.6 pp. 551-557
ISSN:
0140-7783
Subject:
broiler chickens; males; liver; cytochrome P-450; enzyme activity; ionophores; antibiotics; protein synthesis; protein composition; messenger RNA; gene expression; transcription (genetics); chicks; dosage; oral administration; Western blotting; high performance liquid chromatography; caffeine; dapsone; biochemical pathways; gene expression regulation; translation (genetics); drug interactions
Abstract:
... Zhang, L.L., Zhang, J.R., Yu, Z.G., Zhao, J., Mo, F., Jiang, S.X. Effects of ionophores on liver CYP1A and 3A in male broilers. J. vet. Pharmacol. Therap. doi: 10.1111/j.1365‐2885.2010.01168.x. The effects of ionophore antibiotics on the enzyme activity, protein and mRNA expression levels of cytochrome P450 (CYP) isoenzymes were investigated in liver from male Arbor Acres (AA) broiler chicks. Mone ...
DOI:
10.1111/j.1365-2885.2010.01168.x

15. Expression of Endothelin‐1 and Endothelin Receptor A in Canine Ovarian Tumours

Author:
Borzacchiello, G.; Mogavero, S.; Tortorella, G.; Catone, G.; Russo, M.
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. e465
ISSN:
0936-6768
Subject:
Western blotting; animal ovaries; bitches; carcinogenesis; humans; immunohistochemistry; ovarian neoplasms; proteins
Abstract:
... Ovarian tumours have a low incidence in bitch. Endothelin (ET‐1) and endothelin A receptor (ET‐A) are overexpressed in human ovarian cancer. Twenty canine ovarian tumours and five normal samples were first evaluated by western blotting and then immunohistochemically for ET‐1 and ET‐A expression. Seventeen out of twenty tumours were ET‐1 positive. Eight out of twenty tumours were ET‐A immunohistoch ...
DOI:
10.1111/j.1439-0531.2010.01580.x
PubMed:
20210880

16. Expression of hepatitis B small surface antigen in Santalum album embryogenic cell suspension cultures

Author:
Shekhawat, U. K. S.; Ganapathi, T. R.; Srinivas, L.
Source:
Biologia plantarum 2010 v.54 no.4 pp. 720-724
ISSN:
0006-3134
Subject:
Agrobacterium radiobacter; Santalum album; Western blotting; additives; culture media; enzyme-linked immunosorbent assay; genetically modified organisms; growing media; hepatitis B; hepatitis B antigens; kanamycin; reverse transcriptase polymerase chain reaction; surface antigens; trehalose
Abstract:
... Embryogenic cell suspension cultures of Santalum album were transformed with Agrobacterium tumefaciens harboring pD35SHER plant expression vector having hepatitis B small surface antigen (HBsAg) with a C-terminal ER retention signal. The transformed colonies were selected on culture medium supplemented with kanamycin and subsequently the transgenic nature of these colonies was confirmed by PCR ana ...
DOI:
10.1007/s10535-010-0128-6

17. Expression of Interleukin (IL)‐1β and IL‐1 Receptors Genes in the Hypothalamus of Anoestrous Ewes after Lipopolysaccharide Treatment

Author:
Herman, AP; Misztal, T.; Herman, A.
Source:
Reproduction in domestic animals 2010 v.45 no.6 pp. e426
ISSN:
0936-6768
Subject:
Western blotting; ewes; gene expression; genes; hypothalamus; interleukins; lipopolysaccharides; messenger RNA; polymerase chain reaction; quantitative analysis; receptors
Abstract:
... This study was designed to determine the effect of intravenous lipopolysaccharide (LPS) administration on the secretion of interleukin (IL)‐1β and IL‐1 receptors (IL‐1Rs) gene expression in the hypothalamus of anoestrous ewes. Gene expression of IL‐1β and its receptors was assayed by the real‐time polymerase chain reaction. The expression of IL‐1β in the hypothalamus was detected using Western blo ...
DOI:
10.1111/j.1439-0531.2010.01595.x
PubMed:
20345592

18. Functional analysis and molecular characterization of two acetylcholinesterases from the German cockroach, Blattella germanica

Author:
Kim, Y.H.; Choi, J.Y.; Je, Y.H.; Koh, Y.H.; Lee, S.H.
Source:
Insect molecular biology 2010 v.19 no.6 pp. 765-776
ISSN:
0962-1075
Subject:
Blattella germanica; Western blotting; antibodies; central nervous system; disulfide bonds; paraoxon; propoxur; substrate specificity; tail; tissue distribution
Abstract:
... Two acetylcholinesterases (AChEs; BgAChE1 and BgAChE2) from Blattella germanica were functionally expressed using the baculovirus system. Kinetic analysis demonstrated that BgAChE2 had higher catalytic efficiency but lower substrate specificity than BgAChE1. With the exceptions of paraoxon and propoxur, BgAChE1 was generally less sensitive to inhibitors than BgAChE2. Western blot analysis using an ...
DOI:
10.1111/j.1365-2583.2010.01036.x
PubMed:
20738424

19. Functional analysis of Spodoptera litura nucleopolyhedrovirus p49 gene during Autographa californica nucleopolyhedrovirus infection of SpLi-221 cells

Author:
Lin, Tiehao; Yu, Mei; Wu, Wenbi; Yu, Qian; Weng, Qingbei; Yang, Kai; Yuan, Meijin; Pang, Yi
Source:
Virus genes 2010 v.41 no.3 pp. 441-449
ISSN:
0920-8569
Subject:
Autographa californica multiple nucleopolyhedrovirus; Drosophila; Escherichia coli; Spodoptera litura nucleopolyhedrovirus; Western blotting; apoptosis; gene expression; genes; homologous recombination; loci; promoter regions; transposition (genetics)
Abstract:
... The Spodoptera litura nucleopolyhedrovirus (SpltNPV) antiapoptotic gene Splt-p49 is able to rescue replication of a p35-null Autographa californica nucleopolyhedrovirus (AcMNPV) in AcMNPV-permissive Sf9 cells. In this study, an AcMNPV p35 knockout bacmid was generated through ET homologous recombination in Escherichia coli and designated as vAcP³⁵⁻KO. The Splt-p49 gene was transposed into the poly ...
DOI:
10.1007/s11262-010-0520-5

20. Gallic acid induces G2/M phase cell cycle arrest via regulating 14-3-3β release from Cdc25C and Chk2 activation in human bladder transitional carcinoma cells

Author:
Ou, Ting-Tsz; Wang, Chau-Jong; Lee, Yung-Shu; Wu, Cheng-Hsun; Lee, Huei-Jane
Source:
Molecular nutrition & food research 2010 v.54 no.12 pp. 1781-1790
ISSN:
1613-4125
Subject:
gallic acid; bladder; DNA damage; humans; carcinoma; Western blotting; cytoplasm; phosphotransferases (kinases); phosphorylation; cell cycle
Abstract:
... Scope: Cell cycle regulation is a critical issue in cancer treatment. Previously, gallic acid (GA) has been reported to possess anticancer ability. Here, we have evaluated the molecular mechanism of GA on cell cycle modulation in a human bladder transitional carcinoma cell line (TSGH-8301 cell). Methods and results: Using flow cytometer analysis, exposure of the cells to 40 μM GA resulted in a sta ...
DOI:
10.1002/mnfr.201000096
PubMed:
20564478

21. High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice

Author:
Youm, Jung Won; Jeon, Jae Heung; Kim, Hee; Min, Sung Ran; Kim, Mi Sun; Joung, Hyouk; Jeong, Won Joong; Kim, Hyun Soon
Source:
Transgenic research 2010 v.19 no.6 pp. 1099-1108
ISSN:
0962-8819
Subject:
Alzheimer disease; Northern blotting; Southern blotting; Western blotting; agronomic traits; antigens; chloroplasts; enzyme-linked immunosorbent assay; genes; heterologous gene expression; humans; immune response; mice; polymerase chain reaction; protein synthesis; recombinant proteins; tobacco; transcription (genetics); transgenic plants; vaccines
Abstract:
... Plastid transformation has to date been applied to the expression of heterologous genes involved in agronomic traits and to the production of useful recombinant proteins. Here, we report a feasibility study for producing the human β-site APP cleaving enzyme (BACE) via transformation of tobacco chloroplasts. Stable integration of human BACE into the plastome was confirmed by PCR. Genomic Southern b ...
DOI:
10.1007/s11248-010-9383-8
PubMed:
20229285
PubMed Central:
PMC7089353

22. Inhibition of ADP-ribosylation factor-like 6 interacting protein 1 suppresses proliferation and reduces tumor cell invasion in CaSki human cervical cancer cells

Author:
Guo, Fengjie; Liu, Yan; Li, Yalin; Li, Guancheng
Source:
Molecular biology reports 2010 v.37 no.8 pp. 3819-3825
ISSN:
0301-4851
Subject:
transcription factor NF-kappa B; tissue repair; cell movement; cell growth; caspase-9; humans; uterine cervical neoplasms; gene expression regulation; reverse transcriptase polymerase chain reaction; Western blotting; neoplasm cells; small interfering RNA; cell proliferation; interphase; mitogen-activated protein kinase; apoptosis; caspase-3
Abstract:
... ADP-ribosylation factor-like 6 interacting protein 1 (ARL6IP1) is an apoptotic regulator. To investigate the role of ARL6IP1 in human cervical cancer progression, we designed and used short hairpin RNA (shRNA) to inhibit ARL6IP1 expression in CaSki cells and validated its effect on cell proliferation and invasion. Changes in gene expression were analyzed by reverse transcriptase-polymerase chain r ...
DOI:
10.1007/s11033-010-0037-y

23. No H- and L-type cases in Belgium in cattle diagnosed with bovine spongiform encephalopathy (1999-2008) aging seven years and older

Author:
Dobly, Alexandre; Langeveld, Jan; van Keulen, Lucien; Rodeghiero, Caroline; Durand, Stéphanie; Geeroms, Riet; Van Muylem, Patrick; De Sloovere, Jessica; Vanopdenbosch, Emmanuel; Roels, Stefan
Source:
BMC veterinary research 2010 v.6 no.1 pp. 206
ISSN:
1746-6148
Subject:
Western blotting; antibodies; bovine spongiform encephalopathy; cattle; disease outbreaks; food chain; food contamination; molecular weight; monitoring; phenotype; prions; Belgium
Abstract:
... BACKGROUND: The bovine spongiform encephalopathy (BSE) epidemic presented homogeneity of the phenotype. This classical BSE (called C-type) was probably due to the contamination of the food chain by a single prion strain. However, due to the active surveillance and better techniques, two rare variants of BSE have been recently reported in different continents without a clear correlation to the BSE ...
DOI:
10.1186/1746-6148-6-26
PubMed:
20492661
PubMed Central:
PMC2881063

24. Ovine serum biomarkers of early and late phase scrapie

Author:
Batxelli-Molina, Isabelle; Salvetat, Nicolas; Andréoletti, Olivier; Guerrier, Luc; Vicat, Guillaume; Molina, Franck; Mourton-Gilles, Chantal
Source:
BMC veterinary research 2010 v.6 no.1 pp. 229
ISSN:
1746-6148
Subject:
diagnostic techniques; quantitative analysis; Mesocricetus auratus; biomarkers; sheep; gene expression regulation; Western blotting; enzyme-linked immunosorbent assay; scrapie; statistical analysis; molecular weight; blood; prions
Abstract:
... BACKGROUND: Transmissible spongiform encephalopathies are fatal neurodegenerative disease occurring in animals and humans for which no ante-mortem diagnostic test in biological fluids is available. In such pathologies, detection of the pathological form of the prion protein (i.e., the causative factor) in blood is difficult and therefore identification of new biomarkers implicated in the pathway o ...
DOI:
10.1186/1746-6148-6-49
PubMed:
21044301
PubMed Central:
PMC2988006

25. Recombinant expression of human nerve growth factor beta in rabbit bone marrow mesenchymal stem cells

Author:
Fan, Bo-Sheng; Lou, Ji-Yu
Source:
Molecular biology reports 2010 v.37 no.8 pp. 4083-4090
ISSN:
0301-4851
Subject:
Western blotting; bioactive properties; bone marrow; chickens; enzyme-linked immunosorbent assay; ganglia; gene expression; genes; humans; molecular weight; nerve growth factor; polypeptides; rabbits; reverse transcriptase polymerase chain reaction; sensory neurons; stem cells
Abstract:
... Nerve growth factor (NGF) is required for the differentiation and maintenance of sympathetic and sensory neurons. In the present study, the recombinant expression of human nerve growth factor beta (hNGF-β) gene in rabbit bone marrow mesenchymal stem cells (rMSCs) was undertaken. Recombinant vector containing hNGF-β was constructed and transferred into rMSCs, the expressions of the exogenous in rMS ...
DOI:
10.1007/s11033-010-0068-4

26. Role of acidic sphingomyelinase in thymol-mediated dendritic cell death

Author:
Xuan, Nguyen Thi; Shumilina, Ekaterina; Schmid, Evi; Bhavsar, Shefalee K.; Rexhepaj, Rexhep; Götz, Friedrich; Gulbins, Erich; Lang, Florian
Source:
Molecular nutrition & food research 2010 v.54 no.12 pp. 1833-1841
ISSN:
1613-4125
Subject:
antimicrobial properties; immunity; proteins; cell death; DNA fragmentation; immunohistochemistry; thymol; dendritic cells; hosts; Western blotting; mice; bones; cell membranes; ceramides; death
Abstract:
... Scope: Thymol is a component of several plants with antimicrobial activity. Little is known about the effects of thymol on immune cells of the host. This study addressed the effects of thymol on dendritic cells (DCs), regulators of innate and adaptive immunity. Methods and results: Immunohistochemistry, Western blotting and fluorescence-activated cell sorting analysis were performed in bone marrow ...
DOI:
10.1002/mnfr.200900577
PubMed:
20603831

27. Soybean Allergens Affecting North American Patients Identified by 2D Gels and Mass Spectrometry

Author:
Gagnon, Christine; Poysa, Vaino; Cober, Elroy R.; Gleddie, Stephen
Source:
Food analytical methods 2010 v.3 no.4 pp. 363-374
ISSN:
1936-9751
Subject:
foods; hypersensitivity; seeds; soy protein; Americans; allergens; genetic lines; Western blotting; gels; immunoglobulin E; seed storage proteins; soybeans; patients; mass spectrometry; gel electrophoresis
Abstract:
... In this study, we compared the immunoglobulin E immunological reactions of 23 soy-allergic, nine soy-sensitive, and four non-allergic human sera to soybean proteins separated by 1D and 2D gel electrophoresis and screened by Western blot. This method led to novel allergen identification in soybean proteins by tandem mass spectrometry analysis of reactive proteins. Soybean is one of the eight most s ...
DOI:
10.1007/s12161-009-9090-3

28. Protective role of air potato (Dioscorea bulbifera) of yam family in myocardial ischemic reperfusion injury

Author:
Vasanthi, Hannah Rachel; Mukherjee, Subhendu; Ray, Diptarka; Pandian Jayachandran, Karuppiah Shanmugasundara; Lekli, Istvan; Das, Dipak Kumar
Source:
Food & function 2010 v.1 no.3 pp. 278-283
ISSN:
2042-650X
Subject:
Dioscorea bulbifera; Western blotting; apoptosis; bicarbonates; body weight; cardiomyocytes; caspase-3; myocardial infarction; necrosis; pro-apoptotic proteins; protective effect; rats; reperfusion injury; yams
Abstract:
... Hydroalcoholic extract of Dioscorea bulbifera (DB), a yam variety called air potato, was tested for its protective effect on myocardial ischemic/reperfusion (I/R) injury in rats due to apoptosis and necrosis. Myocardial I/R injury was induced by 30 min ischemia followed by 2 h reperfusion by perfusing isolated rat hearts with Krebs Henseilet bicarbonate (KHB) buffer in a Langendorff set up. Pretre ...
DOI:
10.1039/c0fo00048e

29. Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA

Author:
Colwell, Douglas D.; Goater, Cameron P.
Source:
Veterinary parasitology 2010 v.174 no.1-2 pp. 162-165
ISSN:
0304-4017
Subject:
cattle; cattle diseases; Dicrocoelium dendriticum; enzyme-linked immunosorbent assay; Western blotting; antibodies; humoral immunity; disease diagnosis; trematode infections; fecal egg count; Alberta; Saskatchewan
Abstract:
... Apparent changes in the distribution of Dicrocoelium dendriticum in western Canada and the absence of other liver flukes in the area prompted an examination of the humoral responses in naturally infected cattle sharing pasture with heavily infected wild cervids. Western blots using sera from the infected cattle indicated that the cattle had antibodies to various fluke antigens, however the pattern ...
DOI:
10.1016/j.vetpar.2010.08.004

30. Perilipin Overexpression in White Adipose Tissue Induces a Brown Fat-Like Phenotype

Author:
Sawada, Takashi; Miyoshi, Hideaki; Shimada, Kohei; Suzuki, Akira; Okamatsu-Ogura, Yuko; Perfield, James W.; Kondo, Takuma; Nagai, So; Shimizu, Chikara; Yoshioka, Narihito; Greenberg, Andrew S.; Kimura, Kazuhiro; Koike, Takao; Federici, Massimo
ISSN:
1932-6203
Subject:
adipocytes; fatty acids; Western blotting; genes; obesity; heat production; beta oxidation; phenotype; droplet size; insulin resistance; gene overexpression; DNA microarrays; mice; quantitative polymerase chain reaction; proteins; energy expenditure; transgenic animals; white adipose tissue; animal models; lipolysis; droplets
Abstract:
... Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype, we performed additional studies in this tr ...
DOI:
10.1371/journal.pone.0014006
PubMed:
21103377
PubMed Central:
PMC2982838

31. Apelin suppresses apoptosis of human vascular smooth muscle cells via APJ/PI3-K/Akt signaling pathways

Author:
Cui, Rong-Rong; Mao, Ding-An; Yi, Lu; Wang, Cheng; Zhang, Xing-Xing; Xie, Hui; Wu, Xian-Ping; Liao, Xiao-Bo; Zhou, Hua; Meng, Ji-Cai; Yuan, Ling-Qing; Liao, Er-Yuan
Source:
Amino acids 2010 v.39 no.5 pp. 1193-1200
ISSN:
0939-4451
Subject:
G-protein coupled receptors; Western blotting; apoptosis; blood serum; cardiovascular diseases; humans; mitogen-activated protein kinase; models; myocytes; phosphatidylinositol 3-kinase; protein synthesis; signal transduction; small interfering RNA; smooth muscle
Abstract:
... Apoptosis of vascular smooth muscle cells (VSMCs) plays an important role in regulating vascular remodeling during cardiovascular diseases. Apelin is the endogenous ligand for the G-protein-coupled receptor APJ and plays an important role in the cardiovascular system. However, the mechanisms of apelin on apoptosis of VSMCs have not been elucidated. Using a culture of human VSMCs as a model for the ...
DOI:
10.1007/s00726-010-0555-x

32. A single subunit MCM6 from pea forms homohexamer and functions as DNA helicase

Author:
Tran, Ngoc Quang; Dang, Hung Quang; Tuteja, Renu; Tuteja, Narendra
Source:
Plant molecular biology 2010 v.74 no.4-5 pp. 327-336
ISSN:
0167-4412
Subject:
DNA helicases; DNA replication; Western blotting; adenosinetriphosphatase; antibodies; arginine; confocal microscopy; cytosol; enzyme activity; multiprotein complexes; peas; recombinant proteins; zinc finger motif
Abstract:
... The initiation of DNA replication starts from origins and is controlled by a multiprotein complex, which involves about twenty protein factors. One of the important factors is hetrohexameric minichromosome maintenance (MCM2-7) protein complex which is evolutionary conserved and functions as essential replicative helicase for DNA replication. Here we report the isolation and characterization of a s ...
DOI:
10.1007/s11103-010-9675-7
PubMed:
20730596

33. Atypical Scrapie/Nor98 in a Sheep from New Zealand

Author:
Kittelberger, Reinhold; Chaplin, Melanie J.; Simmons, Marion M.; Ramirez-Villaescusa, Ana; McIntyre, Lachlan; MacDiarmid, Stuart C.; Hannah, Michaela J.; Jenner, Judy; Bueno, Rudolfo; Bayliss, Daniele; Black, Hugh; Pigott, Clive J.; O'Keefe, Joseph S.
ISSN:
1040-6387
Subject:
Western blotting; brain stem; cerebellum; enzyme-linked immunosorbent assay; screening; sheep; Europe; New Zealand
Abstract:
... In a consignment of sheep brains from New Zealand, to be used in Europe as negative control material in scrapie rapid screening test evaluations, brain samples from 1 sheep (no. 1512) gave the following initially confusing results in various screening tests: the brainstem repeatedly produced negative results in 2 very similar screening kits (enzyme-linked immunosorbent assay [ELISA]-1, ELISA-2), a ...
DOI:
10.1177/104063871002200604
PubMed:
21088169

34. Bi-Antigenic Immunoassay Models Based on the Recombinant PvpA Proteins for Mycoplasma Gallisepticum Diagnosis in Chickens

Author:
Büyüktanır, Özlem; Genç, Oktay; Yurdusev, Nevzat
ISSN:
1040-6387
Subject:
Mycoplasma gallisepticum; Western blotting; amino acids; chickens; immunoassays; models; molecular weight; proteins; recombinant antigens
Abstract:
... The present study aimed to produce the relatively conserved central fragment of the Mycoplasma gallisepticum PvpA cytadhesin as recombinant antigen and to determine its species-specific diagnostic potential in comparison with the full-length recombinant rPvpA336 protein. For this purpose, a recombinant protein (rPvpA134) consisting of 134 amino acids with apparent molecular mass of 27 kD was produ ...
DOI:
10.1177/104063871002200609
PubMed:
21088174

35. Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle

Author:
Muroya, Susumu; Ertbjerg, Per; Pomponio, Luigi; Christensen, Mette
Source:
Meat science 2010 v.86 no.3 pp. 764-769
ISSN:
0309-1740
Subject:
Western blotting; meat aging; muscle fibers; muscles; myosin heavy chains; pork; protein degradation; proteolysis; swine
Abstract:
... A novel approach was applied in this study to directly evaluate the effect of muscle fiber type on postmortem protein degradation. Porcine muscle fibers were isolated from longissimus muscle at day 1, 3, and 6 postmortem. Fibers were sorted by immunochemical myosin heavy chain isoform typing. Western blot analysis of fibers pooled separately into type I or IIb showed that the relative amounts of 3 ...
DOI:
10.1016/j.meatsci.2010.06.019
PubMed:
20663615

36. Differentially Expressed Outer Membrane Proteins of Vibrio alginolyticus in Response to Six Types of Antibiotics

Author:
Xiong, Xiao-Peng; Wang, Chao; Ye, Ming-Zhi; Yang, Tian-Ci; Peng, Xuan-Xian; Li, Hui
Source:
Marine biotechnology 2010 v.12 no.6 pp. 686-695
ISSN:
1436-2228
Subject:
Crustacea; Vibrio alginolyticus; Western blotting; antibiotic resistance; bacteria; chloramphenicol; erythromycin; fish; gene expression regulation; kanamycin; lipoproteins; nalidixic acid; new drugs; outer membrane proteins; pathogens; porins; screening; streptomycin; tetracycline
Abstract:
... Vibrio alginolyticus is an opportunistic pathogen that occasionally causes life-threatening infections in individuals and results in great losses in marine aquacultures of crustaceans and fish. Recently, antibiotic-resistant strains of the bacterium from clinical and environmental sources have been reported with increasing frequency. However, few reports were involved in the antibiotic resistance ...
DOI:
10.1007/s10126-009-9256-4

37. Enhanced Cytokinin Synthesis in Tobacco Plants Expressing PSARK::IPT Prevents the Degradation of Photosynthetic Protein Complexes During Drought

Author:
Rivero, Rosa M.; Gimeno, Jacinta; Van Deynze, Allen; Walia, Harkamal; Blumwald, Eduardo
Source:
Plant & cell physiology 2010 v.51 no.11 pp. 1929-1941
ISSN:
0032-0781
Subject:
Calvin cycle; NADH or NADPH oxidoreductases; Nicotiana tabacum; Western blotting; abscisic acid; adenosine triphosphate; biosynthesis; brassinosteroids; chlorophyll; cytochromes; cytokinins; drought; drought tolerance; electron transfer; energy transfer; gene expression; genes; messenger RNA; photorespiration; photosystem I; photosystem II; polymerase chain reaction; protein degradation; protein kinases; tobacco; tomatoes; transcriptome; transgenic plants; water stress
Abstract:
... To identify genes associated with the cytokinin-induced enhanced drought tolerance, we analyzed the transcriptome of wild-type and transgenic tobacco (Nicotiana tabacum 'SR1') plants expressing PSARK::IPT (for senescence-associated receptor kinase::isopentenyltransferase) grown under well-watered and prolonged water deficit conditions using the tomato GeneChip. During water deficit, the expression ...
DOI:
10.1093/pcp/pcq143
PubMed:
20871100

38. Estrogenic Response in Male Bullfrog (Rana catesbeiana) Hepatocytes After Single or Combined Exposure to Cadmium (Cd) and 17beta-Estradiol (E2)

Author:
Lee, Keun Woo; Chang, Ziwei; Oh, Beom-Seok; Lu, Ming; Park, Jang-Su
Source:
Bulletin of environmental contamination and toxicology 2010 v.85 no.5 pp. 452-456
ISSN:
0007-4861
Subject:
Lithobates catesbeianus; Western blotting; cadmium; culture media; estradiol; estrogenic properties; heavy metals; hepatocytes; males; oxidative stress; reactive oxygen species; vitellogenesis; vitellogenin
Abstract:
... Contamination by heavy metals and sex hormones in a water environment is an important health issue. In this study, we investigated the estrogenic effects of cadmium (Cd) administration alone and in combination with 17beta-estradiol (E2) on the hepatocytes of male Bullfrog (Rana catesbeiana). Their vitellogenin (VTG) expression and reactive oxygen species (ROS) were analyzed upon exposure to Cd alo ...
DOI:
10.1007/s00128-010-0119-5

39. Expression of lactate transporters MCT1, MCT2 and CD147 in the red blood cells of three horse breeds: Finnhorse, Standardbred and Thoroughbred

Author:
MYKKÄNEN, A. K.; PÖSÖ, A. R.; McGOWAN, C. M.; McKANE, S. A.
Source:
Equine veterinary journal 2010 v.42 Suppl s38 pp. 161-166
ISSN:
0425-1644
Subject:
Finnhorse; Standardbred; Thoroughbred; Western blotting; blood sampling; cell membranes; erythrocytes; lactic acid; monocarboxylic acid transporters; racehorses; racing performance
Abstract:
... Reasons for performing study: In exercising horses, up to 50% of blood lactate is taken up into red blood cells (RBCs). Lactate transporter proteins MCT1, MCT2 and CD147 (an ancillary protein for MCT1) are expressed in the equine RBC membrane. In Standardbreds (SB), lactate transport activity is bimodally distributed and correlates with the amount of MCT1 and CD147. About 75% of SB studied have hi ...
DOI:
10.1111/j.2042-3306.2010.00205.x

40. Generation of Polyclonal Antibodies Against Recombinant Human Glucocerebrosidase Produced in Escherichia coli

Author:
Novo, Juliana Branco; Oliveira, Maria Leonor Sarno; Magalhães, Geraldo Santana; Morganti, Ligia; Raw, Isaías; Ho, Paulo Lee
Source:
Molecular biotechnology 2010 v.46 no.3 pp. 279-286
ISSN:
1073-6085
Subject:
Chinese hamsters; Escherichia coli; Western blotting; affinity chromatography; animal ovaries; antiserum; enzyme-linked immunosorbent assay; glycosylation; human cell lines; humans; mice; molecular weight; monoclonal antibodies; nickel; polyclonal antibodies; therapeutics
Abstract:
... Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher's disease, the most common inherited storage disorder. Treatment consists of enzyme replacement therapy by the administration of recombinant GCR produced in Chinese hamster ovary cells. The production of anti-GCR antibodies has already been described with placenta-derived human GCR that requires successive chromatograph ...
DOI:
10.1007/s12033-010-9303-4

41. Hydrogen sulfide protects against vascular remodeling from endothelial damage

Author:
Vacek, Thomas P.; Gillespie, William; Tyagi, Neetu; Vacek, Jonathan C.; Tyagi, Suresh C.
Source:
Amino acids 2010 v.39 no.5 pp. 1161-1169
ISSN:
0939-4451
Subject:
NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); Western blotting; antioxidants; apoptosis; carotid arteries; catheters; drinking; extracellular matrix; gene expression regulation; hydrogen sulfide; metalloproteinases; mice; oxidative stress; proteins; sodium; superoxide dismutase
Abstract:
... Remodeling by its very nature implied synthesis and degradation of extracellular matrix (ECM) proteins. Although oxidative stress, matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) have been implicated in vascular remodeling, the differential role of MMPs versus TIMPs and oxidative stress in vascular remodeling was unclear. TIMP-3 induced vascular cell apoptosis, ther ...
DOI:
10.1007/s00726-010-0550-2

42. Hypocholesterolemic activity of grape seed proanthocyanidin is mediated by enhancement of bile acid excretion and up-regulation of CYP7A1

Author:
Jiao, Rui; Zhang, Zesheng; Yu, Hongjian; Huang, Yu; Chen, Zhen-Yu
Source:
Journal of nutritional biochemistry 2010 v.21 no.11 pp. 1134-1139
ISSN:
0955-2863
Subject:
quantitative polymerase chain reaction; messenger RNA; liver; grapes; Mesocricetus auratus; enzymes; Western blotting; cholesterol; gene expression; bile; low density lipoprotein; blood; cholesteremic effect; excretion
Abstract:
... Interest in grape seed proanthocyanidin (GSP) as a cholesterol-lowering nutraceutical is growing. This study was to investigate the effect of GSP on blood cholesterol level and gene expression of cholesterol-regulating enzymes in Golden Syrian hamsters maintained on a 0.1% cholesterol diet. Results affirmed supplementation of 0.5% or 1.0% GSP could decrease plasma total cholesterol and triacylglyc ...
DOI:
10.1016/j.jnutbio.2009.10.007
PubMed:
20092993

43. Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells

Author:
Zheng, Guopei; Zhou, Min; Ou, Xinrong; Peng, Bo; Yu, Yanhui; Kong, Fangren; Ouyang, Yongmei; He, Zhimin
Source:
FEBS journal 2010 v.277 no.21 pp. 4506-4518
ISSN:
1742-464X
Subject:
drug therapy; cisplatin; cross resistance; Western blotting; neoplasm cells; mitomycin; paclitaxel; multiple drug resistance; gene expression; gene expression regulation; acetazolamide; caspase-3; oligonucleotides; complementary DNA; humans; tongue; squamous cell carcinoma; prediction; doxorubicin; quantitative polymerase chain reaction; carbonate dehydratase; microarray technology; expressed sequence tags; pH; breast neoplasms
Abstract:
... Drug resistance is the major obstacle to successful cancer treatment. To understand the mechanisms responsible for drug resistance in tongue cancer, Tca8113 cells derived from moderately differentiated human tongue squamous cell carcinoma were exposed to stepwise escalated concentrations of pingyangmycin (PYM) to develop the resistant cell line called Tca8113/PYM, which showed over 18.78-fold incr ...
DOI:
10.1111/j.1742-4658.2010.07836.x
PubMed:
21040473

44. Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi

Author:
Pang, H.-Y.; Li, Y.; Wu, Z.-H.; Jian, J.-C.; Lu, Y.-S.; Cai, S.-H.
Source:
Journal of applied microbiology 2010 v.109 no.5 pp. 1800-1809
ISSN:
1364-5072
Subject:
ABC transporters; Epinephelus coioides; Vibrio harveyi; Western blotting; antigens; antiserum; fish; immune response; isoelectric focusing; outer membrane proteins; two-dimensional gel electrophoresis; vaccination; vaccines
Abstract:
... The main aim of this study was to screen novel immunogenic proteins of Vibrio harveyi, which could be vaccine candidates. Whole-cell proteins of V. harveyi, strain Li01 and Huang01, were first separated by isoelectric focusing, followed by 2D-PAGE, respectively. Immunogenic proteins were identified by Western blotting, using Epinephelus coioides antisera against V. harveyi strain Li01. Western blo ...
DOI:
10.1111/j.1365-2672.2010.04808.x
PubMed:
20698911

45. Inflammatory Myopathy with Severe Tongue Atrophy in Pembroke Welsh Corgi Dogs

Author:
Toyoda, Kaoru; Uchida, Kazuyuki; Matsuki, Naoaki; Sakai, Hideo; Kitagawa, Masato; Saito, Miyoko; Sasaki, Jun; Nakayama, Hiroyuki
ISSN:
1040-6387
Subject:
Western blotting; autoantibodies; biopsy; breeds; dog diseases; dogs; etiology; histopathology; muscles; muscular atrophy; muscular diseases; skeletal muscle; striated muscle; tongue; Japan
Abstract:
... A disease characterized by tongue and facial muscle atrophy has been recognized sporadically among Pembroke Welsh Corgi (PWC) dogs in Japan. The present study describes the pathologic findings of this canine syndrome. Histopathologic examinations were performed in 2 dogs, including a case of muscular biopsy. Identification and characterization of autoantibodies were attempted by fluorescent antibo ...
DOI:
10.1177/104063871002200605
PubMed:
21088170

46. Influence of cell surface properties on adhesion ability of bifidobacteria

Author:
Wang, Li-Qun; Meng, Xiang-Chen; Zhang, Bai-Rong; Wang, Yan; Shang, Yu-Lin
Source:
World journal of microbiology & biotechnology 2010 v.26 no.11 pp. 1999-2007
ISSN:
0959-3993
Subject:
Bifidobacterium; Western blotting; adhesion; adverse effects; human cell lines; humans; hydrophobicity; intestinal mucosa; lithium chloride; pathogens; pepsin; screening; surface proteins
Abstract:
... Adhesion ability of bifidobacteria to the intestinal mucosa is considered to be the prerequisite for colonization of bifidobacteria and can protect against gastrointestinal pathogens infection. The aim of this study was to investigate bifidobacterial surface traits related to adhesion ability in vitro and characterize the cell surface substances that may be involved in the adhesion process of bifi ...
DOI:
10.1007/s11274-010-0384-9

47. Inhibitory effect of panduratin A isolated from Kaempferia panduarata Roxb. on melanin biosynthesis

Author:
Lee, Chan Woo; Kim, Han Sung; Kim, Han Kon; Kim, Jin‐Woong; Yoon, Ji Hoon; Cho, Yumi; Hwang, Jae Kwan
Source:
Phytotherapy research 2010 v.24 no.11 pp. 1600-1604
ISSN:
0951-418X
Subject:
Kaempferia; Western blotting; biosynthesis; enzymes; melanin; melanocytes
Abstract:
... Hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, thus melanin synthesis inhibitors have been of great interest as target molecules for cosmetic and medicinal purposes. The aim of this study was to investigate the in vitro inhibitory effect of panduratin A, isolated from Kaempferia pandurata Roxb., on melanogenesis and its related enzymes such as tyrosinase ...
DOI:
10.1002/ptr.3163
PubMed:
21031615

48. Kaempferol induced apoptosis via endoplasmic reticulum stress and mitochondria‐dependent pathway in human osteosarcoma U‐2 OS cells

Author:
Huang, Wen‐Wen; Chiu, Yu‐Jen; Fan, Ming‐Jen; Lu, Hsu‐Feng; Yeh, Hsiu‐Feng; Li, Kun‐Hong; Chen, Po‐Yuan; Chung, Jing‐Gung; Yang, Jai‐ Sing
Source:
Molecular nutrition & food research 2010 v.54 no.11 pp. 1585-1595
ISSN:
1613-4125
Subject:
DNA; DNA damage; Western blotting; animal disease models; anticarcinogenic activity; apoptosis; cytotoxicity; endoplasmic reticulum; enzyme inhibitors; flow cytometry; gel electrophoresis; human cell lines; kaempferol; membrane potential; mice; osteoblasts; osteosarcoma; oxidative stress; protein synthesis; signal transduction; stem cells; viability
Abstract:
... Kaempferol is a natural flavonoid. Previous studies have reported that kaempferol has anti‐proliferation activities and induces apoptosis in many cancer cell lines. However, there are no reports on human osteosarcoma. In this study, we investigate the anti‐cancer effects and molecular mechanisms of kaempferol in human osteosarcoma cells. Our results demonstrate that kaempferol significantly reduce ...
DOI:
10.1002/mnfr.201000005
PubMed:
20564475

49. Microbial production of palatinose through extracellular expression of a sucrose isomerase from Enterobacter sp. FMB-1 in Lactococcus lactis MG1363

Author:
Park, Jong-Yul; Jung, Jong-Hyun; Seo, Dong-Ho; Ha, Suk-Jin; Yoon, Jong-Won; Kim, Young-Cheul; Shim, Jae-Hoon; Park, Cheon-Seok
Source:
Bioresource technology 2010 v.101 no.22 pp. 8828-8833
ISSN:
0960-8524
Subject:
Enterobacter; Lactococcus lactis; Western blotting; affinity chromatography; enzymes; genes; glucose; isomaltulose; isomers; pH; signal peptide; sucrose
Abstract:
... Sucrose isomerase (SIase) has been used to produce palatinose, a structural isomer of sucrose, which has many beneficial health properties, such as low-glycemic and low-insulinemic indices. A gene corresponding to SIase from Enterobacter sp. FMB-1 was expressed in Lactococcus lactis MG1363 using the P170 expression system. The autoinducible promoter (P170) and an optimized signal peptide (SP310mut ...
DOI:
10.1016/j.biortech.2010.06.068
PubMed:
20620050

50. Monoclonal antibodies against Stx1B subunit of Escherichia coli O157:H7 distinguish the bacterium from other bacteria

Author:
Li, G.; Hong, J.; Huo, G.; Ren, X.
Source:
Letters in applied microbiology 2010 v.51 no.5 pp. 499-503
ISSN:
0266-8254
Subject:
Escherichia coli O157; Listeria monocytogenes; Mycobacterium tuberculosis; Salmonella; Shiga-like toxins; Staphylococcus aureus; Streptococcus agalactiae; Western blotting; bacteria; enteritis; enzyme-linked immunosorbent assay; hybridomas; monitoring; monoclonal antibodies; virulence
Abstract:
... The Shiga-like toxins (Stx) are critical virulence factors of enterohaemorrhagic Escherichia coli (EHEC). Stx1B subunit plays important roles in EHEC infection. This work aims to generate and characterize monoclonal antibodies (mAbs) against the Stx1B and to investigate their utility in discrimination ELISA. Two newly identified mAbs (designated 2H8 and 1B10, respectively) against the Stx1B protei ...
DOI:
10.1111/j.1472-765X.2010.02919.x
PubMed:
20854398