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- de Andrade, André Furugen Cesar, et al. Show all 8 Authors
- Animal reproduction science 2010 v.120 no.1-4 pp. 31-38
- semen extenders; viability; cryopreservation; cattle; flow cytometry; automation; cryoprotectants; processing equipment; sperm motility; male fertility; mitochondria; acrosome; Gir (cattle breed); plasma membrane; germplasm conservation; computer software
- ... The objectives of the present study were to investigate the effects of three equilibration times (0, 2, and 4h) and two extenders (TRIS or Bioxcell®) for cryopreservation of bull semen. Semen from 12 Gyr bulls was cryopreserved using an automated freezing machine. There were significant interactions between equilibration times and extenders for sperm motility and membrane integrity. The control tr ...