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- Schaffer, David V., et al. Show all 2 Author
- Trends in biotechnology 2010 v.28 no.3 pp. 117-124
- engineering; population; stem cells
- ... The stem cell niche is an anatomical site that contains a reservoir of multipotent stem cells (SCs) that can maintain normal tissue, or replenish injured or aged cell populations, in response to mechanisms that regulate whether they should remain quiescent, undergo self-renewal, or differentiate. The choice among these hallmark SC behaviors is governed by intricate soluble and “solid phase” signal ...
- Schaffer, David V., et al. Show all 4 Authors
- Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.28 pp. 12475-12480
- Murine leukemia virus; RNA-directed DNA polymerase; carcinogenesis; engineering; gene expression; genome; humans; insertional mutagenesis; mutants; retroviral vectors; transcription (genetics); zinc finger motif
- ... Retroviral vectors offer benefits of efficient delivery and stable gene expression; however, their clinical use raises the concerns of insertional mutagenesis and potential oncogenesis due to genomic integration preferences in transcriptional start sites (TSS). We have shifted the integration preferences of retroviral vectors by generating a library of viral variants with a DNA-binding domain inse ...
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