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- Federico, Rodolfo, et al. Show all 4 Authors
- Analytical and bioanalytical chemistry 2010 v.396 no.3 pp. 1281-1290
- Lathyrus sativus; diamine oxidase; enzyme activity; gels; image analysis; oxidation; pH; pancreatin; pepsin; peroxidase; polyacrylamide; polyacrylamide gel electrophoresis; proteolysis; putrescine; seedlings; sodium dodecyl sulfate
- ... A zymographic assay of diamine oxidase (DAO, histaminase, EC 220.127.116.11), based on a coupled peroxidase reaction, and its behavior at proteolysis in simulated gastric and intestinal conditions, are described. The DAO activity from a vegetal extract of Lathyrus sativus seedlings was directly determined on sodium dodecyl sulfate polyacrylamide electrophoretic gels containing entrapped horseradish perox ...
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