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water pollution; cadmium; zinc; Microcystis aeruginosa; acclimation; metal tolerance; toxicity; uptake mechanisms; subcellular fractions; bioaccumulation
Abstract:
... To understand the metal tolerance of a bloom-forming cyanobacterium, Microcystis aeruginosa, we investigated its acclimation to and recovery from cadmium (Cd) and zinc (Zn) exposure. The intracellular Cd and Zn (intra-Cd and intra-Zn) quotas increased upon acclimation to increased metal concentrations and were reduced following 1-day or 5-day recovery. Different acclimation to varying metal concen ...
Microcystis aeruginosa; Phormidium; Planktothrix; Synechococcus; World Health Organization; cross reaction; drinking water; enzyme-linked immunosorbent assay; health hazards; high performance liquid chromatography; lakes; microcystins; quantitative analysis; surface water; Turkey (country)
Abstract:
... Dissolved (extracellular) microcystin (MC) concentrations were determined at 3 sampling stations on Lake Kovada, Turkey. The dominant species of cyanobacteria found in August and September of 2006 were Microcystis aeruginosa, Synechococcus sp., Phormidium limosum, Phormidium formosa and Planktothrix limnetica. MC concentrations in water were measured by ELISA and MC variants were examined by HPLC- ...
Microcystis aeruginosa; microalgae; cadmium; zinc; heavy metals; water pollution; uptake mechanisms; bioaccumulation; toxicity; prediction; models
Abstract:
... Microcystis aeruginosa is an important cyanobacteria widely found in numerous freshwater lakes. In this study, we examined the uptake and toxic effects of cadmium (Cd) and zinc (Zn) in non-toxic (Ma 469) and toxic (Ma 905) strains of Microcystis based on the metal free ion and cellular concentrations. We compared their uptake rates, pulse-amplitude-modulated parameters (maximum photosystem II quan ...
... The present study evaluated the effect of naturally developing cyanobacteria on the composition of muscles of two commercially important freshwater fish species. Fish were exposed to cyanobacterial biomass including Microcystis aeruginosa and Microcystis ichthyoblabe for 4 weeks. Then, they were transferred to dechlorinated potable water without any cyanobacteria for another 4-week period, thus mo ...
... The algistatic properties of aqueous barley straw (Hordeum vulgare) extracts have been observed in laboratory studies and in situ. This reported algistatic property has been used by farmers and horticulturists to control algal blooms in various systems and has become standard practice in some areas. However, both inhibition and stimulation of algal growth in freshwater and marine species have been ...
... So far, the presence of microcystins in Portuguese freshwater resources has always been attributed to the bloom-forming cyanobacteria Microcystis aeruginosa. In 2005, however, microcystins were detected at the Beliche reservoir (Algarve, South Portugal), following the development of a bloom dominated by Planktothrix rubescens. The identity of the causative organism was confirmed by combining both ...
... Photosynthetic inorganic carbon utilization was investigated in two cyanobacteria Microcystis aeruginosa and Synechocystis PCC6803 grown in standing culture. Photosynthetic rates for the two algae reached about 10 times the theoretical CO₂ supply rate at low dissolved inorganic carbon (DIC) of 100 μmol/L, and the rates were unaffected by the addition of 20 mmol/L Na⁺, indicating that the two algae ...
... We isolated and identified strains of marine and freshwater planktonic and benthic microalgae from the vicinity of Indian River County, Florida (≈27.5°N, 80.34°W), cultivated them in batch culture, and examined their allelopathic activity against mosquito larvae. Additional algal material was obtained from Syracuse University and the University of Texas—Austin Algal Culture Collection. Mosquito la ...
... Allelochemical ethyl 2-methyl acetoacetate (EMA) can significantly inhibit the growth of bloom-forming Microcystis aeruginosa. In order to assess the implication of the damage of EMA on the algal photosynthetic apparatus, the effects of EMA on the algal ultrastructure and pigment composition were investigated. At initial exposure time to EMA (0-40 h), algal allophycocyanin, phycoerythrin and carot ...
... Arsenic pollution and eutrophication are both prominent issues in the aquaculture ponds of Taiwan. It is important to study the effects of arsenic on algal growth and toxin production in order to assess the ecological risk of arsenic pollution, or at least to understand naturally occurring ponds. The sensitivity of algae to arsenate has often been linked to the structural similarities between arse ...
... The antialgal activity of newly synthesized bacillamides against several cyanobacteria and microalgae isolates was screened using a rapid 96-well microplate bioassay. Cultures were exposed to serial dilutions of each bacillamide derivative (0-160 μg mL⁻¹) in the microplate wells and daily optical measurements were used to estimate growth over a 216 h period. Inhibition values (%) were calculated f ...
... Although nitrate is a macronutrient and can serve as good nitrogen source for many species of phytoplankton, high nitrate concentrations do not benefit the growth of phytoplankton. We hypothesise that algae cultured under high nitrate concentrations can accumulate intracellular nitrite, which is produced by nitrate reductase (NR) and can inhibit the growth of algae. To assess the validity of this ...
... The presence of cyanobacterial hepatotoxins such as microcystin-LR poses health threats to humans due to their potential for causing severe physiological effects when contaminated drinking water is ingested. Here, the electrochemical detection of microcystin-LR is explored using a quantum dot/antibody (QD/Ab) probe for nanoparticle-based amplification and direct electrochemical transduction. The i ...
... Several cyanobacterial species have a high potential to dominate in marine environments and freshwater reservoirs, and the ecological and physiological reasons for this phenomenon are not understood comprehensively. In this study, the ability of a Microcystis aeruginosa Kütz. strain to produce free dissolved enzymes was documented. We have observed that this highly toxic strain releases alkaline p ...
water pollution; plant exudates; phytoplankton; endocrine-disrupting chemicals; in vitro culture; xenobiotics; phenols; toxicity testing; microbial growth; hydrolysis; extraction; Microcystis aeruginosa; Portugal
Abstract:
... Octylphenol (OP) is a xenobiotic with endocrine disrupting properties found in freshwaters worldwide. Its effects have been studied in organisms with nuclear receptors but effects on phytoplankton communities are poorly characterized, despite the fact that these organisms are constantly exposed to this compound. For this reason fate and effects of OP in the cyanobacterium Microcystis aeruginosa we ...
... 1. We investigated the role of algal composition on pumping, clearance, assimilation, pseudofaeces and faeces production, feeding time budgets, and condition of zebra mussels from spring to autumn at two sites in Saginaw Bay (Lake Huron) and one site in western Lake Erie. Size-fractioned chlorophyll was used to distinguish between feeding on small (<53 μm) and large (>53 μm) size fractions, and mu ...
... In recent years, the exploration and development of the effective methods of treatment and prevention to algal blooms, especially Microcystis aeruginosa blooms has been an important issue in the field of water environment protection. Allelochemicals (natural plant toxins) are considered promising sources of algicides to control algal blooms. The objective of this study is to determine the inhibito ...
... To explore the potential grazing effects of mussels on Microcystis aeruginosa, a common bloom-forming phytoplankton, Unio douglasiae and Corbicula fluminea were fed with Scenedesmus obliquus, toxic and non-toxic strains of Microcystis aeruginosa as single food and as mixtures in the laboratory. When fed with single foods, U. douglasiae has similar clearance rates on the three algae populations, wh ...
... To better understand the production of microcystins (MCs) in Microcystis colonies, fluorescence in situ hybridization (FISH) methods were developed to detect DNA involved in the synthesis of these cyanobacterial hepatotoxins. Using colonies of Microcystis aeruginosa (Kütz.) Kütz. isolated from environmental blooms of cyanobacteria and from a colony-forming, MC-producing laboratory strain of Microc ...
... We investigated microcystin (MC) production by three Microcystis aeruginosa strains in response to different kairomone concentrations (indirect exposure: 0%, 10%, 25%, and 50%) of Hypophthalmichthys molitrix and Carassius gibelio langsdorfi. The MC production was significantly different among control and three kairomone treatment levels. MC production was higher with increasing concentration of ka ...
Eurytemora affinis; Microcystis aeruginosa; acute toxicity; dietary exposure; estuaries; fish; fish feeds; lethal concentration 50; long term effects; microcystin-LR; risk; zooplankton
Abstract:
... This study investigates the toxicity and post-exposure effects of dissolved microcystin (MC-LR) on the dominant copepods of the upper San Francisco Estuary (SFE), where blooms of the toxic cyanobacteria Microcystis aeruginosa coincide with record low levels in the abundance of pelagic organisms including phytoplankton, zooplankton, and fish. The potential negative impact of Microcystis on the cope ...
... PCR-based denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments was used to identify the cyanobacterial phylotypes in sediments and plankton of saline-alkaline and freshwater lakes of Kenya. The detection of the aminotransferase domain located on modules mcyE and ndaF using specific molecular markers confirmed the presence of potential toxin-producing cyanobacteria. Th ...
DNA; Microcystis aeruginosa; biosynthesis; chemotypes; cluster analysis; genes; lakes; matrix-assisted laser desorption-ionization mass spectrometry; microcystins; phylogeny; rivers; water supply; Portugal
Abstract:
... Strains of the cyanobacterium Microcystis aeruginosa were isolated into pure culture from a variety of lakes, rivers, and reservoirs in Portugal. Samples were tested with matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) to investigate the presence of various peptide groups including aeruginosins, microginins, anabaenopeptins, cyanopeptilins, microcystins, ...
... Phosphorus (P) transfer between Microcystis aeruginosa and the attached bacterium Pseudomonas was studied using radioactive P (³²P) and green fluorescence protein-labeled Pseudomonas. M. aeruginosa in P-starved condition took up most ³²P (70%) in water and about 50% of ³²P in ³²P-saturated bacteria in individual experiments. However, only 26% of ³²P in the ³²P-saturated M. aeruginosa was transferr ...
... A recent study has shown that nonanoic acid (NA) is one of the strongest allelochemicals to a cyanobacterium Microcystis aeruginosa, but the physiological responses of M. aeruginosa to NA stress remain unknown. In this study, physiological characters such as the growth rate, photosynthetic processes, phosphorus and nitrogen uptake kinetics, and the contents of intracellular microcystin of M. aerug ...
nutrient content; cations; watersheds; seasonal variation; Microcystis aeruginosa; groundwater; biochemical oxygen demand; zooplankton; interspecific variation; lakes; Mesocyclops; multivariate analysis; surface water; water quality; community structure; species diversity; chemical oxygen demand; Brachionus; silicates; physicochemical properties; environmental factors; rain; nitrates; India
Abstract:
... Old Fort Lake, a small (1.6 ha), shallow, and recreational water body in Delhi (India) was studied through monthly surveys in two consecutive years (January, 2000-December, 2001). Precipitation is the major source of water for this closed basin lake. In addition, ground water is used for replenishing the lake regularly. This alkaline, hyposaline hard water lake contains very high ionic concentrati ...
... We examined the selective inhibitory potential of rice hull extract on the toxic cyanobacterium Microcystis aeruginosa, in comparison with inhibitory effects on two green algae (Ankistrodesmus convolutus and Scenedesmus quadricauda) and a zooplankton (Daphnia magna) species. The inhibitory effect of rice hull extract, measured by algal growth or zooplankton survival using four different concentrat ...
... Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper ...
... In order to determine the short-term impact induced by salt stress, cultures of Dictyosphaerium chlorelloides and Microcystis aeruginosa were grown in presence of increasing sea-salt concentrations. Growth rate and photosystem II activity in D. chlorelloides, and photosynthetic oxygen production (in both species) were analyzed. A concentration-dependent response was obtained with the presence of s ...
... The elemental composition of primary producers reflects the availability of light, carbon and nutrients in their environment. According to the carbon-nutrient balance hypothesis, this has implications for the production of secondary metabolites. To test this hypothesis, we investigated a family of toxins, known as microcystins, produced by harmful cyanobacteria. The strain Microcystis aeruginosa H ...
... 1. In order to test the effect of Ochromonas sp., a mixotrophic chrysophyte, on cyanobacteria, grazing experiments were performed under controlled conditions. We studied grazing on three Microcystis aeruginosa strains, varying in toxicity and morphology, as well as on one filamentous cyanobacterium, Pseudanabaena sp. Furthermore, we analysed the co-occurrence of Ochromonas and Microcystis in natur ...
Microcystis aeruginosa; phosphorus; chemical concentration; toxicity; cadmium; zinc; uptake mechanisms
Abstract:
... In the present study, we quantified the 4-h uptake and 48-h toxicity of Cd and Zn in a freshwater cyanobacterium, Microcystis aeruginosa, under varied cellular and ambient P concentrations. After acclimation under different P conditions, the cyanobacteria had different cellular P concentrations. We found that an elevated cellular P concentration significantly promoted the short-term uptake of Cd a ...
Chironomidae; Diatoma; Microcystis aeruginosa; Tubificidae; Ulothrix; alkalinity; allelopathy; dissolved oxygen; enzyme-linked immunosorbent assay; flora; genes; genetic markers; lakes; littoral zone; macroinvertebrates; pH; photosynthesis; toxicity; water quality; watersheds; South Africa
Abstract:
... The biological interactions and the physical and chemical properties of the littoral zone of Lake Krugersdrift were studied for a 4-month period when a dense, toxic cyanobacterial bloom dominated by Microcystis aeruginosa was present in the main lake basin. The presence of a toxic strain of M. aeruginosa was confirmed through the use of ELISA and molecular markers that detect the presence of the m ...