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- Cairrão, E.; Santos-Silva, A. J.; Alvarez, E.; Correia, I.; Verde, I.
- In vitro cellular & developmental biology 2009 v.45 no.3-4 pp. 175-184
- umbilical arteries, etc ; actin; calcium channels; collagenase; contractile proteins; cultured cells; elastase; electrophysiology; endothelial cells; fibroblasts; humans; myocytes; myosin heavy chains; smooth muscle; umbilical cord; Show all 15 Subjects
- ... The human umbilical cord is a biological sample that can be easily obtained just after birth. A methodology was developed to perform cultures of human umbilical artery smooth muscle cells (HUASMC) expressing contractile proteins and functional ionic channels. To avoid fibroblast and endothelial cell contamination, we mechanically separated the tunica media, which only contains HUASMC and matrix pr ...