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- Jong, Yuh-Jyh, et al. Show all 4 Authors
- Electrophoresis 2009 v.30 no.7 pp. 1102-1110
- DNA; cellulose; electrophoresis; fluorescence; genes; genotyping; high performance liquid chromatography; muscular atrophy; neurons; parents; patients; polymerase chain reaction; screening; sequence analysis
- ... We established a universal multiplex PCR and CE to calculate the copy number of survival motor neuron (SMN1 and SMN2) genes for clinical screening of spinal muscular atrophy (SMA). In this study, one universal fluorescent primer was designed and applied for multiplex PCR of SMN1, SMN2 and two internal standards (CYBB and KRIT1). These amplicons were separated by conformation sensitive CE. Mixture ...