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- Lin, Win-Li, et al. Show all 10 Authors
- Electrophoresis 2009 v.30 no.2 pp. 410-416
- DNA; DNA methylation; cost effectiveness; digestion; electrophoresis; epigenetics; genes; genomic islands; polymerase chain reaction; restriction endonucleases
- ... In this article, we show that methylation-specific multiplex PCR (MS-multiplex PCR) is a sensitive and specific single assay for detecting CpG methylation status as well as copy number aberrations. We used MS-multiplex PCR to simultaneously amplify three sequences: the 3' ends of the SNRPN gene (for unmethylated sequences), the KRITI gene (as internal control), and the promoter of the SNRPN gene c ...