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- Platt, H. W. (Bud), et al. Show all 4 Authors
- Plant molecular biology reporter 2009 v.27 no.3 pp. 315-320
- gene expression; reference standards; reverse transcriptase polymerase chain reaction; Arabidopsis thaliana; genes; Solanum tuberosum; Phytophthora infestans; fungal diseases of plants
- ... Analysis of gene expression using real-time reverse transcription polymerase chain reaction (RT-PCR) requires reference genes to normalize expression values between samples. We have developed a novel reference for real-time RT-PCR using an arbitrary primer to amplify a random set of genes. The arbitrary primer amplifies over 30 genes, whose cumulative expression as measured by real-time RT-PCR clo ...