... We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) using seven structural genes and 108 sequenced amplified fragment length polymorphis ...
... Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants. ...
... Many animals and plants have symbiotic relationships with beneficial bacteria. Experimentally tractable models are necessary to understand the processes involved in the selective transmission of symbiotic bacteria. One such model is the transmission of the insect-pathogenic bacterial symbionts Photorhabdus spp. by Heterorhabditis bacteriophora infective juvenile (IJ)-stage nematodes. By observing ...
Escherichia coli; Streptomyces avermitilis; enzymes; galactooligosaccharides; galactose; genes; hydrolysis; open reading frames; polysaccharides; prokaryotic cells; proteins; secretion; signal peptide; tomatoes
Abstract:
... The putative endo-β-1,6-galactanase gene from Streptomyces avermitilis was cloned and expressed in Escherichia coli, and the enzymatic properties of the recombinant enzyme were characterized. The gene consisted of a 1,476-bp open reading frame and encoded a 491-amino-acid protein, comprising an N-terminal secretion signal sequence and glycoside hydrolase family 5 catalytic module. The recombinant ...
... Hiracin JM79 (HirJM79), a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, was cloned and produced in Lactococcus lactis, Lactobacillus sakei, Enterococcus faecium, Enterococcus faecalis, and Pichia pastoris. For heterologous production of HirJM79 in lactic acid bacteria (LAB), the HirJM79 structural gene (hirJM79), with or without the HirJM79 immunity gene (hiriJM79), was cloned int ...
stilbenes; Rheum palmatum; procyanidins; high performance liquid chromatography; mass spectrometry; rhubarb; spectral analysis; chemical constituents of plants; naphthalene; anthraquinones
Various methodologies have been used to estimate and map percent impervious surface area (%ISA) using moderate resolution remote sensing imagery (e.g., Landsat Thematic Mapper). There is, however, a lack of comparative analyses among these methods. This study compares three major spectral analysis techniques (regression modeling, regression tree, and normalized spectral mixture analysis (NSM ...
... rRNA-based studies, which have become the most common method for assessing microbial communities, rely upon faithful amplification of the corresponding genes from the original DNA sample. We report here an analysis and reevaluation of commonly used primers for amplifying the DNA between positions 27 and 1492 of bacterial 16S rRNA genes (numbered according to the Escherichia coli rRNA). We propose ...
raw materials; methodology; isolation techniques; hydrolysis; Cronobacter sakazakii; factories
Abstract:
... A differential medium, "Cronobacter" screening broth, has been designed to complement agars based on hydrolysis of chromogenic α-glucopyranoside substrates. The broth was evaluated using 329 Enterobacteriaceae strains (229 target isolates), spiked/naturally contaminated samples, and a parallel comparison with current methods for raw materials, line/end products, and factory environment samples. ...
... An Escherichia coli galactose kinase gene knockout (ΔgalK) strain, which contains the L-arabinose isomerase gene (araA) to isomerize D-galactose to D-tagatose, showed a high conversion yield of tagatose compared with the original galK strain because galactose was not metabolized by endogenous galactose kinase. In whole cells of the ΔgalK strain, the isomerase-catalyzed reaction exhibited an equili ...
... Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on the growth of and neurotoxin production by nonproteolytic Clostridium botulinum type E were studied during the growth cycle. Quantitative reverse transcription-PCR and an enzyme-linked immunosorbent assay were used to quantify expression of the type E botuli ...
The diversity of data sources, analysis methodologies, and classification systems has led to a number of new techniques for monitoring land-cover change. However, this wide choice means that it is difficult to know which solution to choose. A system capable of integrating the results of different analyses and applying them to land-cover mapping would therefore be extremely useful. This study ...
... The increased serum survival gene iss has long been recognized for its role in extraintestinal pathogenic Escherichia coli (ExPEC) virulence. iss has been identified as a distinguishing trait of avian ExPEC but not of human ExPEC. This gene has been localized to large virulence plasmids and shares strong similarities with the bor gene from bacteriophage λ. Here, we demonstrate that three alleles o ...
linear models; topography; uncertainty; vegetation
Abstract:
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Much effort has been spent on examining the spatial variation of classification accuracy and associated factors on a per-pixel basis. In the past few years, object-based classification has attracted growing interest. This paper examines factors affecting the spatial variation of classification uncertainty in an object-based vegetation mapping. We studied six categories of factors in an objec ...