An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... 3, 4-Dihydroxybenzaldehydeisonicotinoylhydrazone was prepared, characterized with spectral analyses and used for developing a new method for the simple, sensitive and rapid spectrophotometric determination of vanadium(V) which gives maximum absorbance at wave length 360 nm. The metal ion gives a yellow colored complex with 3, 4-DHBINH in acetate buffer of pH 5.5 with 1:1 (metal:ligand) composition ...
gas production (biological); in vitro digestion; liquids; feed concentrates; ruminants; ruminant nutrition; rumen microorganisms; rumen fermentation; propionates; acetates; butyrates; forage evaluation; inoculum; volatile fatty acids; batch systems
Abstract:
... A simple and inexpensive in vitro feed evaluation system which estimates microbial fermentation by measuring gas production and allows for adjustment of liquid dilution rate has been design. Working conditions, in terms of substrate and inoculum proportions (experiment 1, EXP 1) and the liquid replacement schedule (experiment 2, EXP 2) were initially set. In EXP 1, three substrate concentrations ( ...
... A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reaction. The flavour esters isobutyl acetate, methyl butyrate, ethyl butyrate and benz ...
... Eosinophils and other phagocytes use NADPH oxidase to kill bacteria. Proton channels in human eosinophils and neutrophils are thought to sustain NADPH oxidase activity, and their opening is greatly enhanced by a variety of NADPH oxidase activators, including phorbol myristate acetate (PMA). In nonphagocytic cells that lack NADPH oxidase, no clear effect of PMA on proton channels has been reported. ...
acetates; carbon; composite polymers; gel chromatography; maleic anhydrides; molecular weight; nuclear magnetic resonance spectroscopy; stable isotopes; styrene
Abstract:
... A new synthetic methodology for the preparation of copolymers having high incorporation of 1-alkene together with multifunctionalities has been developed by polarity-activated reversible addition-fragmentation chain transfer (RAFT) copolymerization. This approach provides well-defined alternating poly(1-decene-alt-maleic anhydride), expanding the monomer types for living copolymerizations. Althoug ...
... This study examined the effects of brewers yeast, fructooligosaccharide (FOS), and GroBiotic®-A, a mixture of partially autolyzed brewers yeast, dairy components and dried fermentation products, on the intestinal microbial community of red drum, Sciaenops ocellatus. Gastrointestinal (GI) tracts were aseptically removed from three sub-adult red drum previously maintained on a commercial diet and pl ...
... An analytical method based on ion-interaction chromatography with UV detection for simultaneous in-vitro estimation of the percutaneous absorption of the most used water-soluble UV filters in sunscreen cosmetics is proposed. These UV filters were phenylbenzimidazole sulfonic acid, disodium phenyl dibenzimidazole tetrasulfonate, benzophenone-4, and terephthalylidene dicamphor sulfonic acid. The met ...
... In this report accelerated weathering characteristics of a novel poly(phenylene ether) (PPE)-based thermoplastic elastomer (TPE), i.e. a blend of styrene–ethylene–butylenes–styrene (SEBS)/ethylene vinyl acetate (EVA) and PPE-polystyrene (PS), were studied in detail. Exterior and interior accelerated weathering protocols were followed during photooxidative weathering to simulate outdoor and indoor ...
acetates; fermentation; gases; hydrogen production; mass transfer; mixed culture
Abstract:
... Gas circulation and fed-batch fermentation were applied for enhancing acetate production by mixed culture in a novel syntrophic acetogenesis and homoacetogenesis coupling system. The results show that the acetate yield in the fed-batch test with gas circulation is about 47% higher than that in the batch test without gas circulation. The fed-batch method helps to increase acetate yield by balancing ...
... Phenylanthraquinones belong to the quite rare class of fully unsymmetric biaryls, consisting of two different molecular portions, an anthraquinone part, chrysophanol, and a phenyl part, 2,4-dihydroxy-6-methoxyacetophenone, linked together by phenol-oxidative coupling. The biosynthesis of these two moieties, from eight and four acetate units, respectively, bears some unique features: Chrysophanol i ...
... Palladium-catalyzed transformations of aryl halides and pseudo-halides involving carbonylation, carbon–carbon and carbon–heteroatom bond-forming reactions, etc. are very well documented, and have already been reviewed several times. The metal-catalyzed activation of benzylic derivatives is much less described. However, during the last decades, a number of papers have shown the interest offered by ...
... This work reports the extraction procedures of alkannin/shikonin mixture from roots of six populations of Onosma echioides, by means of three extraction techniques: Soxhlet extraction, maceration and rapid solid-liquid dynamic extraction (RSLDE). Five solvents with different polarity (hexane, petroleum ether, chloroform, ethyl acetate, methanol) were also studied. Analysis of the extracts was perf ...
... The chloroplast ATP synthase gates the flow of protons out of the thylakoid lumen. In Chlamydomonas reinhardtii deletion of any of the genes for the ATP synthase subunits, or misfolding of the peptides results in photosynthetic membranes devoid of the enzyme (Lemaire and Wollman, J Biol Chem 264:675-685, 1989). This work examines the physiologic response of an algal strain in which the epsilon sub ...
... IL-10-secreting regulatory T cell lines specific to glatiramer acetate [poly(Y,E,A,K)n] or poly(Y,F,A,K)n have been established from the enlarged spleen and lymph nodes that result from copolymer treatment of SJL mice in which experimental autoimmune encephalomyelitis was induced by PLP139-151. These CD4+CD25+T cell lines secrete high levels of IL-10 and IL-13 but only small amounts of IL-4 and vi ...
... Adults and larvae of the thrips Callococcithrips fuscipennis (Moulton) (Thysanoptera: Tubulifera: Phlaeothripidae) live in the sticky wax masses of adult females of the felt scale insect Callococcus acaciae (Maskell) (Sternorrhyncha: Coccoidea: Eriococcidae). The scale is sessile and feeds on Kunzea shrubs (Myrtales: Myrtaceae). If stressed, the thrips produce droplets of secretions. The mixture c ...
... Rod-shaped Alphaproteobacteria possessing long prosthecae-like appendages have been detected abundantly in biofilms attaching onto anode graphite of cellulose-fed microbial fuel cells (MFCs). To identify their ecological roles, the present study isolated a corresponding bacterium (strain Mfc52) by direct plating of a biofilm suspension onto a solid medium containing glucose and ferric ion. Phyloge ...
... BACKGROUND: A single-stage reactor, in which the growth of bacterial culture, induction of desulfurizing enzymes, and desulfurization reaction are carried out in a single step, was adopted to investigate desulfurization of dibenzothiophene (DBT) at high cell densities. Rhodococcus erythropolis, IGTS8 was used as the biocatalyst. Optimal conditions for bacterial growth and DBT desulfurization were ...
... This study investigated the anaerobic degradation of the polycyclic aromatic hydrocarbons (PAHs) phenanthrene and pyrene in mangrove sediment from Taiwan. The anaerobic degradation of PAH was enhanced by the addition of acetate, lactate, pyruvate, sodium chloride, cellulose, or zero-valent iron. However, it was inhibited by the addition of humic acid, di-(2-ethylhexyl) phthalate (DEHP), nonylpheno ...
... Acidogenesis of food waste was studied in a 2-L reactor with semi-continuous mode operation (once-a-day feeding and draw-off) for maximum 65 days to examine optimal volatile acid compositions for biological nitrogen removal (BNR) and enhanced biological phosphorus removal (ENPR). Various operational parameters of hydraulic retention time (HRT), organic loading rate (ORL), pH and temperature were i ...
... In this study, chemical compositions of hydrodistilled essential oil and anti-inflammatory activities from the twigs of Cinnamomum osmophloeum Kaneh. were investigated for the first time. The chemical constituents of the twig essential oil were further analyzed by GC-MS and they were found to be l-bornyl acetate (15.89%), caryophyllene oxide (12.98%), γ-eudesmol (8.03%), β-caryophyllene (6.60%), T ...
... The antibacterial properties of the three seagrasses namely Cymodocea serrulata, Halophila ovalis and Zostera capensis were tested against the human pathogens Staphylococcus aureus, Bacillus cereus, B. subtilis, Escherichia coli, Salmonella paratyphi, Salmonella typhimurium and Micrococcus luteus, using six different solvents namely, petroleum ether, chloroform, ethyl acetate, acetone, methanol an ...
... A screening for larvicidal activity of plant extracts with some known medicinal attributes could lead to the discovery of new agents for pest and vector control. In the backdrop of recent revival of interest in developing plant-based insecticides, the present study was carried out to evaluate the larvicidal properties in three medicinal plants growing abundantly in the region of Chitheri Hills, Dh ...
... Marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. This study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. Nine representative strains were isolated from a range of surfaces and were grown in YEB medium ...
... This paper reports the isolation from soil of Penicillium strain PY-1 with strong antagonistic activity against plant pathogenic fungi. On the basis of its morphological characteristics and the sequence of the ITS region, strain PY-1 was identified as P. oxalicum. Strain PY-1 produces antifungal substances that suppress the mycelial growth of Sclerotinia sclerotiorum and many other plant pathogeni ...
... In this study, in vitro and in vivo antiinflammatory activities of fruits from Lindera erythrocarpa Makino were evaluated. The ethyl acetate soluble fraction derived from the ethanol extract of L. erythrocarpa fruits inhibited significantly nitric oxide (NO) production in lipopolysaccharide (LPS) induced NO in the murine macrophage cell line (RAW264.7) assay, the EC₅₀ being 16.35 μg/mL. Four compo ...
... This study investigates the effects of N-(n-dodecyl)diethanolamine (DDA) on enzymes and growing cells of Escherichia coli NCIMB 8277. Enzyme activities in the presence of DDA were determined by measuring substrate-dependent oxygen consumption by whole cells, or of NADH formation or oxidation by cell extracts. Lysis of growing cells was followed by measuring changes in turbidity and cell count. DDA ...
... Antimicrobial activity of methanolic extracts obtained from the aerial parts of Evax pygmaea was tested against five bacteria and two strains of phytopathogenic fungi using the agar diffusion and broth microdilution methods. Antioxidant properties were evaluated through the ability of the different fractions to scavenge the stable ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) and D ...
... Antimicrobial, antioxidant, and antiviral activities of flower extracts of Retama raetam Forssk. Webb (Fabaceae) were screened both from standard and isolated Gram-positive and Gram-negative bacteria by solid medium technique. Oxacillin, Amoxicillin, Ticarcillin, Cefotaxim, and Amphotericin were used as the control agents. The antiviral activity was evaluated against human cytomegalovirus (HCMV) s ...
... The search for natural antioxidants, especially of plant origin, has notably increased in recent years. Bunium persicum Boiss. is an economically important medicinal plant growing wild in the dry temperature regions in Iran. In this study, chemical constituents of the essential oil of the seed from Bunium persicum Boiss. have been studied by GC/MS technique. The major components were caryophyllene ...
... Cinnamomum osmophloeum Kaneh. is an indigenous tree species in Taiwan. In this study, phytochemical characteristics and antioxidant activities of ethanolic extracts from the twigs of C. osmophloeum were investigated for the first time. The extracts were obtained successively using liquid-liquid partition to yield n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (BuOH) and water fractions. The anti ...
... Acid hydrolysis of almond shells was carried out with 2.5 and 5% sulfuric acid, and the ethyl acetate soluble fraction of hydrolysates (EASH) was fractionated on a Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) column. Three fractions were recovered from the crude extract produced by hydrolysis with 2.5% sulfuric acid (EASH-2.5), and five from the one produced with 5% sulfuric acid (EASH-5 ...
... The liquors from steam explosion of Olea europea wood carried out at temperatures in the range of 190-240°C were analysed for phenolic content, radical scavenging capacity and reducing power. Increased ethyl acetate solubles (EAS) and phenolics [as Gallic Acid Equivalents, (GAE)] extraction yield with increasing temperature were observed. At the higher temperature tested, up to 2.3 g EAS/100 g dry ...
... The present study was designed to explore the antioxidant potential of chloroform, ethyl acetate, n-butanol and water fractions of 80% methanol extract of leaves Chukrasia tabularis by 2,2'-diphenyl-1-picrylhydrazyl (DPPH), deoxyribose degradation (non-site specific and site specific), reducing power and DNA nicking assays. The different fractions showed significant activities in all the free radi ...
... In vitro antioxidant activities of three selected Indian red seaweeds - viz., Euchema kappaphycus, Gracilaria edulis and Acanthophora spicifera were evaluated. Total phenolic content and reducing power of crude methanol extract were determined. The antioxidant activities of total methanol extract and five different solvent fractions (viz., petroleum ether (PE), ethyl acetate (EA), dichloromethane ...
epicatechin; chemical composition; acetates; Dimocarpus longan; proanthocyanidins; methanol; extraction; plant extracts; flowers; solvents; hexane; chemical constituents of plants; antioxidant activity
Abstract:
... Three different solvent extracts (methanol, ethyl acetate, and n-hexane) of longan (Dimocarpus longan Lour.) flowers were assayed with three different antioxidant capacity methods, namely, the DPPH free radical scavenging effect, the oxygen radical absorbance capacity (ORAC) assay, and the inhibition of Cu2+-induced oxidation of human low-density lipoprotein (LDL). It was revealed that the methano ...
... The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography-mass spectrometry (GC-MS) has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as ...
... Adsorptive stripping voltammetry is a very sensitive and selective method for determination of drugs and organic substances in biological fluids. We have shown that determination of testosterone by adsorptive stripping voltammetry is possible using a lead film electrode. The lead film plating process and accumulation of testosterone were performed simultaneously from an acetate buffer solution of ...
... Blood sausages are traditional products in many parts of the world. In most cases, a very short shelf-life limits their consumption to the areas in which they are produced. In this work, different mild preservation methods were applied to Morcilla de Burgos, a Spanish blood sausage, consisting of a range of organic acid salts (OAS) and high-pressure processing (HPP), with the aim of increasing its ...
... The Archaea diversity was evaluated in an agricultural biogas plant supplied with cattle liquid manure and maize silage under mesophilic conditions. Two different genes (16S rRNA; methyl-coenzyme-M-reductase, MCR) targeted by three different PCR primer sets were selected and used for the construction of three clone libraries comprising between 104 and 118 clones. The clone libraries were analysed ...
acetates; consumer acceptance; decanoic acid; discriminant analysis; gas chromatography; hexanoic acid; isoamyl alcohol; linear models; octanoic acid; odor compounds; odors; principal component analysis; sensory evaluation; wine quality; wines
Abstract:
... In order to investigate the relationship between sensory and gas chromatographic analysis data, and to establish if particular aroma compounds could be used as Malvazija istarska young wine quality markers, 30 samples of Malvazija istarska young wine were subjected to sensory evaluation and gas chromatographic analysis of aroma compounds. Linear regression and principal component analysis were app ...
... This study was conducted to determine the composition of essential oil and headspace from Elsholtzia splendens, and to determine the aroma-active compounds of this aromatic plant. Eighty-five components were identified in the oil by GC and GC-MS. The major compounds were naginata ketone (70.43%) and elsholtzia ketone (4.95%). Sixty-eight volatile flavour constituents were detected in the headspace ...
... Fuller's rose weevil (FRW; Pantomorus cervinus) (Coleoptera: Curculionidae), a polyphagous pest of citrus and other horticultural plants, was studied in laboratory assays designed to identify potential semiochemicals from host plants that might be exploited for weevil pest management. Using still-air bioassays, weevils were found to be most attracted to fresh whole lemon leaves compared with cut a ...
... Two groups of 108 Angus cross yearling heifers were utilised to determine the effects of growth-promoting agents on behaviour, when utilised under thermoneutral, hot and cold environmental conditions. Pens of heifers were observed throughout the day for feed intake pattern via bunk score, panting (hot), degree of bunching and shivering (cold). For cattle that were exposed to cold stress, feed inta ...
Cephus cinctus; acetates; adults; air; air flow; beta-ocimene; bioassays; females; grasses; males; oviposition; pest management; sawflies; stems; volatile compounds; wheat
Abstract:
... 1 Adult wheat stem sawflies Cephus cinctus, pests of cultivated cereals that also infests wild grasses, migrate into wheat fields where they oviposit in elongating, succulent stems. 2 Volatiles released by wheat plants at susceptible stages were analyzed to determine potential semiochemical compounds. Seven major compounds were identified and quantified. 3 A Y-tube bioassay was developed to evalua ...
... This study is focused on the biodegradation of saline tannery effluent by identified halotolerant bacterial consortia in a bench-scale reaction calorimeter. A satisfactory agreement was observed between oxygen uptake rate profiles and heat flux–time curves confirming that, under strict aerobic conditions, calorimetry and respirometry provided the same information. Oxycalorific equivalent determine ...
... The continuous aerobic degradation of phenol, mixed with readily degradable synthetic wastewater was studied over a period of 400 days at 25±5°C temperature in a fixed bed biofilm reactor using 'Liapor' clay beads as packing material. The phenol concentration added to the reactor ranged from 0.19 to 5.17g/l and was achieved by a gradual increase of phenol in wastewater, thus adapting the microbial ...
... Most of our understanding of the physiology of microorganisms is the result of investigations in pure culture. However, in order to understand complex environmental processes, there is a need to investigate mixed microbial communities. This is true for enhanced biological phosphorus removal (EBPR), an environmental process that results in the enrichment of the polyphosphate-accumulating organism A ...
... Biofilm formation and adherence properties of 13 bacterial strains commonly found in wastewater treatment systems were studied in pure and mixed cultures using a crystal violet microtiter plate assay. Four different culture media were used, wastewater, acetate medium, glucose medium and diluted nutrient broth. The medium composition strongly affected biofilm formation. All strains were able to for ...
... Biodegradation kinetic behaviors of ethyl acetate and amyl acetate in a composite bead biofilter were investigated. The composite bead was the spherical PVA/peat/KNO₃/GAC composite bead which was prepared in our previous works. Both microbial growth rate and biochemical reaction rate were inhibited at higher inlet concentration. For the microbial growth process, the microbial growth rate of ethyl ...
... Hydrogen may be considered a potential fuel for the future since it is carbon-free and oxidized to water as a combustion product. Bioconversion of synthesis gas (syngas) to hydrogen was demonstrated in continuous stirred tank bioreactor (CSTBR) utilizing acetate as a carbon source. An anaerobic photosynthetic bacterium, Rhodospirillum rubrum catalyzed water-gas shift reaction which was applied for ...
... The biokinetics of glucose metabolism were evaluated in Aeromonas hydrophila during growth in an anaerobic biosystem. After approx 34 h growth, A. hydrophila metabolized 5,000 mg glucose l⁻¹ into the end-products ethanol, acetate, succinate and formate. The maximum growth rate, μ m, half saturation coefficients, K s, microbial yield coefficient, Y, cell mass decay rate coefficient, k d, and substr ...
... This work conducted a denitrifying sulfide removal (DSR) test in an expanded granular sludge bed (EGSB) reactor at sustainable loadings of 6.09 kg m⁻³ day⁻¹ for sulfide, 3.11 kg m⁻³ day⁻¹ for nitrate–nitrogen, and 3.27 kg m⁻¹ day⁻¹ for acetate–carbon with >93% efficiency, which is significantly higher than those reported in literature. Strains Pseudomonas sp., Nitrincola sp., and Azoarcus sp. very ...
... The potential to produce and accumulate mono- and sesquiterpenes in lemon mint (Mentha aquatica var. citrata) glandular trichomes was evaluated. Volatiles were extracted using methyl-tert-butyl ether or solid phase micro extraction and determined by gas chromatography-mass spectrometry. The main components of the essential oil of lemon mint are the monoterpene alcohol linalool ...
... Saxitoxin (STX) and its analogues cause the paralytic shellfish poisoning (PSP) syndrome, which afflicts human health and impacts coastal shellfish economies worldwide. PSP toxins are unique alkaloids, being produced by both prokaryotes and eukaryotes. Here we describe a candidate PSP toxin biosynthesis gene cluster (sxt) from Cylindrospermopsis raciborskii T3. The saxitoxin biosynthetic pathway i ...
... The microbial transformation of β-amyrin acetate (compound 1) occurred in the culture of Rhodobacter sphaeroides producing three metabolites. The metabolites were purified and identified as β-amyrin (compound 2), (3β)-olean-12-ene-3, 23-diol (compound 3), and erythrodiol (compound 4). Initially, it was found that β-amyrin acetate could be deacetylated and hydroxylated by microbial methods. ...
... This study investigated extracellular electron shuttle-mediated RDX biodegradation and the distribution of ring cleavage metabolites generated by biological degradation (cells) versus the products formed by abiotic degradation (reduced electron shuttles), and when the two pathways were acting simultaneously. All pathways were influenced by pH. Buffered suspensions (pH 6.8/7.9/9.2) were performed w ...
... The effects of short-chain fatty acids (butyrate, propionate, and acetate) and trichostatin A (TSA), a typical histone deacetylase inhibitor, on tumor necrosis factor (TNF)-α secretion and nuclear factor κB (NF-κB) activation in peripheral blood mononuclear cells induced with lipopolysaccharide were evaluated in relation to prostaglandin E₂ (PGE₂) secretion. Treatment of cells with butyrate; tribu ...
... A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0–10.0. Excess reagent was extracted with n-hexan ...
... BACKGROUND AND AIMS: Studies on xylogenesis focus essentially on the stem, whereas there is basically no information about the intra-annual growth of other parts of the tree. As roots strongly influence carbon allocation and tree development, knowledge of the dynamics of xylem production and maturation in roots at a short time scale is required for a better understanding of the phenomenon of tree ...
Kartal, Boran; van Niftrik, Laura; Rattray, Jayne; van de Vossenberg, Jack L.C.M.; Schmid, Markus C.; Sinninghe Damsté, Jaap; Jetten, Mike S.M.; Strous, Marc
... Anaerobic ammonium oxidizing (anammox) bacteria are detected in many natural ecosystems and wastewater treatment plants worldwide. This study describes the enrichment of anammox bacteria in the presence of acetate. The results obtained extend the concept that the anammox bacteria can be enriched to high densities in the presence of substrates for heterotrophic growth. Batch experiments showed that ...
... A macrocyclic polyamine, 1,5,9,13,17,21,25,29-octaazacyclodotriacontane ([32]ane-N₈), in the bonded phase was employed as a molecular receptor for CEC separation of oligopeptides. Parameters affecting the performance of the separations were considered. Baseline separation for the mixture of angiotensin I, angiotensin II, [Sar¹, Thr⁸]-angiotensin II, β-casomorphin bovine, β-casomorphin human, oxyto ...
... An assay for the simultaneous determination of the enantiomers of hydroxymebendazole (OH-MBZ) and hydroxyaminomebendazole (OH-AMBZ) together with aminomebendazole (AMBZ) in human plasma is described for the first time. It is based upon liquid-liquid extraction at alkaline pH from 0.5 mL plasma followed by analysis of the reconstituted extract by CE with reversed polarity in the presence of a 50 mM ...
... The electrophoretic properties of boron cluster compounds were determined in water, methanol and ACN as solvents of the BGE and discussed based on the principles of ion migration. Two types of boron cluster compounds were investigated. One type consisted of derivatives of the nido-7,8-dicarbaundecaborate cluster, the other types are derivatized cobalt bis(dicarbollide) ions (COSANs) whose central ...
... A moderately acid-tolerant, malodorous bacterium, strain FP, was isolated from peat that had a pore water pH of c. 4.2. The 16S rRNA gene sequence of FP was closely related to that of acetogens Clostridium drakei, Clostridium scatologenes, and Clostridium carboxidivorans. The DNA-DNA reassociation values obtained with DNA from FP and that of these three acetogens approximated 80%, 64%, and 59%, re ...
Anogeissus; Leishmania; Terminalia; acetates; fractionation; high performance liquid chromatography; nuclear magnetic resonance spectroscopy; parasitoses; tannins
Abstract:
... Stem barks of Anogeissus leiocarpus and Terminalia avicennoides widely used in Africa for treatment of some parasitic diseases were collected and made into methanolic extracts. The extracts were tested on four strains of promastigote forms of Leishmania in vitro. Solvent fractionation in aqueous, butanolic, and ethyl acetate layer indicated butanol and aqueous fractions to have a superior leishman ...
... The ability of Pdᴵᴵ complexes derived from 2,6-pyridinedicarboxylic acids to catalyze homogeneous regioselective aerobic oxidation of 5- and 6-substituted 8-methylquinolines in AcOH–Ac₂O solution to produce corresponding 8-quinolylmethyl acetates in high yield was demonstrated; corresponding 8-quinoline carboxylic acids are minor reaction products. ...
acetates; bismuth; matrix-assisted laser desorption-ionization mass spectrometry; molecular weight; nuclear magnetic resonance spectroscopy; polymerization; polymers; salts
Abstract:
... Acidic bismuth salts, such as BiCl₃, BiBr₃, BiJ₃, and Bi-triflate catalyzed the ring-opening polymerization of 2-methoxazoline (MOZ) in bulk at 100 °C, whereas less acidic salts such as Bi₂O₃ or Bi(III)acetate did not. Bi-triflate-catalyzed polymerizations of 2-ethyloxazoline (EtOZ) were performed with variation of the monomer-catalyst ratio (M/C). It was found that the molecular weights were inde ...
... Bifidobacterium bifidum, in contrast to other bifidobacterial species, is auxotrophic for N-acetylglucosamine. Growth experiments revealed assimilation of radiolabelled N-acetylglucosamine in bacterial cell walls and in acetate, an end-product of central metabolism via the bifidobacterial d-fructose-6-phosphate shunt. While supplementation with fructose led to reduced N-acetylglucosamine assimilat ...
... Polyhydroxyalkanoate (PHA) copolymers comprising the four monomers 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 3-hydroxy-2-methylvalerate (3HMV) and 3-hydroxy-2-methylbutyrate (3HMB) were generated using the recently discovered Defluviicoccus vanus-related glycogen accumulating organisms (DvGAOs) under anaerobic conditions without applying any nutrient limitations. The composition could be m ...
acetates; electrospray ionization mass spectrometry; high performance liquid chromatography; progesterone
Abstract:
... The fragmentation behaviors of progesterone derivatives were studied by high-performance liquid chromatography electrospray ionization tandem mass spectrometry. Under tandem MS conditions, most of the fragments were formed by the cleavage of peripheral groups. Analyses of the fragmentation pathways revealed that the presence of substituents of a progesterone derivative could be deduced from charac ...
acetates; chemical composition; derivatization; emulsions; mass spectrometry; optical properties; polymers; resins; ultraviolet radiation; Spain
Abstract:
... Polyvinyl resins, in particular poly(vinyl acetate) emulsions (PVA), are widely used in contemporary paintings as binding media, because of the optimum mechanical and optical properties these polymers confer on the paint layers. A study has been carried out to chemically characterize samples of PVA resins prepared as coating films from commercial products currently available from fine arts supplie ...
... We describe an approach for characterizing selective binding between oppositely charged ionic functional groups under biologically relevant conditions. Relative shifts in K-shell x-ray absorption spectra of aqueous cations and carboxylate anions indicate the corresponding binding strengths via perturbations of carbonyl antibonding orbitals. XAS spectra measured for aqueous formate and acetate solu ...
... The ArcB/A two-component signal transduction system of Escherichia coli modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the putative arcA and arcB genes of Mannheimia succiniciproducens MBEL55E, a capnophilic (CO₂-loving) rumen bacterium, encode functional proteins that specify a two-component system. The Arc proteins of the two bacterial ...
benzaldehyde; acetates; lactones; whisky; phenols; headspace analysis; pyrazines; butanol; odor compounds; extracts; odors; ethanol; eugenol; United States
Abstract:
... Application of the aroma extract dilution analysis (AEDA) on the volatile fraction carefully isolated from an American Bourbon whisky revealed 45 odor-active areas in the flavor dilution (FD) factor range of 32-4096 among which (E)-β-damascenone and δ-nonalactone showed the highest FD factors of 4096 and 2048, respectively. With FD factors of 1024, (3S,4S)-cis-whiskylactone, γ-decalactone, 4-allyl ...
Cryptomeria japonica; acetates; acetic acid; drying; gas chromatography; kilns; mass spectrometry; steam; temperature; terpenoids; wood; wood drying; wood extractives
Abstract:
... Sawdusts of sugi (Cryptomeria japonica) wood prepared before and after steam drying at 120°C in a kiln were extracted with n-hexane and ethyl acetate to give n-hexane extracts and ethyl acetate extracts. From gas chromatography-mass spectrometry analysis of the ethyl acetate extracts from woods before and after steam drying, the components of 4-epi-cubebol, cubebol, and 2,7(14),10-bis-abolatrien-1 ...
... The labial gland secretions from males of the North American bumblebees Bombus morrisoni Cresson and B. rufocinctus Cresson were analyzed by gas chromatography/mass spectrometry. In both species, 3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenyl acetate was found as the major compound of a complex mixture of alkenols, acetates, hydrocarbons, and wax-type esters. In addition to a mixture of saturat ...
... Many of the herbal extracts used in the Chinese clinical medical routine inhibit the growth of tumor cells. In the present work, extracts of 12 selected herbs were prepared with methanol, chloroform, ethyl acetate and water, and the effects of these on the multidrug resistance (MDR) and P-glycoprotein of mouse lymphoma cells transfected with the human mdr1 gene and on a human lung alveolar epithel ...
... Enantiomer separations were performed on three β-cyclodextrin-based chiral stationary phases (CSP) containing the pernaphthylcarbamoylated β-cyclodextrin (CSP 1), peracetylated β-cyclodextrin (CSP 2) and permethylated β-cyclodextrin (CSP 3) as chiral selectors by capillary liquid chromatography and pressure-assisted capillary electrochromatography in this study. Triethylammonium acetate/MeOH or ph ...
Muller, Alexander J.; Sharma, Madhav D.; Chandler, Phillip R.; DuHadaway, James B.; Everhart, Mary E.; Johnson, Burles A. III.; Kahler, David J.; Pihkala, Jeanene; Soler, Alejandro Peralta; Munn, David H.; Prendergast, George C.; Mellor, Andrew L.
... Topical application of phorbol myristate acetate (PMA) elicits intense local inflammation that facilitates outgrowth of premalignant lesions in skin after carcinogen exposure. The inflammatory response to PMA treatment activates immune stimulatory mechanisms. However, we show here that PMA exposure also induces plasmacytoid dendritic cells (pDCs) in local draining lymph nodes (dLNs) to express ind ...
... BACKGROUND: D-ribose, a five carbon sugar, can be produced via mutant strains with impairment in the non-oxidative part of the pentose phosphate pathway (PPP). D-ribose is an important intermediate in the pharmaceutical industry. This work focuses on the effect of various substrates on D-ribose production with a transketolase deficient strain of Bacillus pumilus IFO13322.RESULT: D-ribose titer is ...
... A comparative study of two strains of Lactobacillus plantarum (REB1 and MLBPL1) grown in commercial medium (MRS broth), cucumber juice, and liquid pig feed was performed to explore changes to the metabolic pathways of these bacteria, using a proteomics approach (two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry) combined with analyses of fermentable sugars and ferm ...
... The interaction of Cu²⁺ ions with the homopolymer poly(styrene sulfonic acid) (PSSH), as well as with the copolymers of maleic acid (MAc) with styrene sulfonic acid (SSH) or vinyl acetate (VAc), was investigated in dilute aqueous solution through turbidimetry, potentiometry, viscometry, and spectrophotometry in the visible region. Cu²⁺ ions were introduced either through neutralization with Cu(OH) ...
... Fungi are the dominant organisms decomposing leaf litter in streams and mediating energy transfer to other trophic levels. However, less is known about their role in decomposing submerged wood. This study provides the first estimates of fungal production on wood and compares the importance of fungi in the decomposition of submerged wood versus that of leaves at the ecosystem scale. We determined f ...
acetates; acetic acid; chlorogenic acid; coffee beans; densitometry; formic acid; high performance liquid chromatography; silica gel
Abstract:
... Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used. Densitometric determination of chlorogenic a ...
plant exudates; chemical composition; acetates; monosaccharides; methylation; chemical analysis; tree trunk; alditols; Prunus persica; arabinogalactans; fruit trees; chemical structure
Abstract:
... The peach tree (Prunus persica) produces gum exudates on its trunk and fruit. The former was extracted with water to give polysaccharide (PPN) and residual material was extracted with alkali giving structurally similar PPNA (NMR examination), which was formed in a slightly higher yield (42% compared with 37%). PPNA consisted of Ara, Xyl, Man, Gal, and uronic acids in a 36:7:2:42:13 molar ratio and ...
Archaea; Dendrosporobacter; acetates; bioreactors; chemical analysis; denaturing gradient gel electrophoresis; fermentation; methane production; methanogens; microbial communities; oligonucleotide probes; oxidation; population structure; propionic acid; sulfate-reducing bacteria; sulfates
Abstract:
... The microbial population structure and function of natural anaerobic communities maintained in lab-scale continuously stirred tank reactors at different lactate to sulfate ratios and in the absence of sulfate were analyzed using an integrated approach of molecular techniques and chemical analysis. The population structure, determined by denaturing gradient gel electrophoresis and by the use of oli ...
... Acetate, propionate, and butyrate, collectively referred to as volatile fatty acids (VFA), are considered among the most important electron donors for sulfate-reducing bacteria (SRB) and heterotrophic nitrate-reducing bacteria (hNRB) in oil fields. Samples obtained from a field in the Neuquén Basin, western Argentina, had significant activity of mesophilic SRB, hNRB, and nitrate-reducing, sulfide- ...
... A kinetic model for the anaerobic filter (AF) that takes into account the mass fractions of sulfate-reducing bacteria (SRB) (f SRB) and methanogenic bacteria (MB) (f MB) and an inhibiting effect of H₂S on bacterial groups is proposed. When the acetate-fed AFs were maintained at the low organic loading rate of 2.5kg COD/m³ d, variations of the influent [formula removed] ratio (0.5-3.0) does not mat ...
chemical composition; grasses; metabolism; acetates; image analysis; stems; cell walls; wood; tension wood; Populus trichocarpa; stem cells; cell wall components; polysaccharides; mass spectrometry; Liliopsida; lignin; leaves
Abstract:
... Acylesterification is one of the common modifications of cell wall non-cellulosic polysaccharides and/or lignin primarily in monocot plants. We analyzed the cell-wall acylesters of black cottonwood (Populus trichocarpa Torr. & Gray) with liquid chromatography-mass spectrometry (LC-MS), Fourier transform-infrared (FT-IR) microspectroscopy, and synchrotron infrared (IR) imaging facility. The results ...
... The objective of the present study was to construct a system that re-creates the conditions of fermentation and absorption of the human proximal colon. The model was constructed using a glass tube with an internal dialysis membrane tube. The food substrate was fed into the dialysis membrane three times a day simulating a typical human feeding. The substrate contained 58% carbohydrates, 35% protein ...
... Controlled radical polymerization of N-vinylcaprolactam (NVCL) via reversible addition-fragmentation chain transfer (RAFT) polymerization or macromolecular design via the interchange of xanthate (MADIX) was described, employing 2-diphenylthiocarbamoylsulfanyl-2-methyl-propionic acid (CTA1), ((O-ethylxanthyl)methyl)benzene (CTA2) and (1-(O-ethylxanthyl)ethyl)benzene (CTA3) as chain transfer agents ...
... The performance of the most common and also some other less common CE buffers has been tested for the pKa determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to 11.8. Whereas some buffers, like acetic acid/acetate, BisTrisH⁺/BisTris, TrisH⁺/Tris, CHES/CHES⁻, and CAPS/CAPS⁻ can be used with all type of analytes, others like a ...
acetates; adenoma; clinical trials; curcumin; curry; humans; lead
Abstract:
... The ability of the curry constituent curcumin to delay the onset of cancer has been the topic of extensive research for many years. Abundant literature is devoted to mechanisms by which curcumin may mediate this activity. These insights have prompted investigations in which curcumin as lead molecule serves as a scaffold for synthetic chemical attempts to optimize pharmacological potency. Among the ...
... Maternal hyperthermia induces pre-implantation embryo death, which is accompanied by enhanced physiological oxidative stress. We evaluated whether the administration of DL-α-tocopherol acetate (TA) to hyperthermic mothers mitigated pre-implantation embryo death. Mice were exposed to heat stress (35 °C, 60% relative humidity) for 12 h or not heated (25 °C) on the day of mating. Twelve hours before ...
... Herbivore-induced volatile organic compounds (VOCs) are widely appreciated as an indirect defense mechanism since carnivorous arthropods use VOCs as cues for host localization and then attack herbivores. Another function of VOCs is plant–plant signaling. That VOCs elicit defensive responses in neighboring plants has been reported from various species, and different compounds have been found to be ...