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- Zeng, Run-Ying, et al. Show all 2 Author
- Marine biotechnology 2008 v.10 no.1 pp. 75-82
- EDTA (chelating agent); Nocardiopsis; alpha-amylase; calcium; cobalt; copper; enzyme activity; enzyme stability; glucose; hydrolysates; magnesium; maltose; maltotriose; manganese; marine sediments; mercury; molecular weight; nucleotide sequences; pH; polyacrylamide gel electrophoresis; ribosomal RNA; sequence analysis; starch; temperature; Antarctic region
- ... An actinomycete strain 7326 producing cold-adapted α-amylase was isolated from the deep sea sediment of Prydz Bay, Antarctic. It was identified as Nocardiopsis based on morphology, 16S rRNA gene sequence analysis, and physiological and biochemical characteristics. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram activity staining of purified amylase showed a single band equal ...
- Zeng, Run-ying, et al. Show all 2 Author
- Marine biotechnology 2008 v.10 no.5 pp. 612-621
- Escherichia coli; Pseudomonas; calcium; chromatography; genes; molecular cloning; molecular weight; nucleotide sequences; pH; psychrotrophic bacteria; sediments; serine; temperature; triacylglycerol lipase; triacylglycerols; tributyrin; Antarctic region
- ... A psychrotrophic bacterium producing a cold-adapted lipase was isolated from the deep-sea sediment of Prydz Bay, Antarctic and identified as a Pseudomonas strain. Determination of the nucleotide sequence of the gene encoding a lipase from Pseudomonas sp. 7323 (lipA) revealed that LipA is composed of 617 amino acid residues with a calculated molecular weight of 64,466 Da. LipA has a GXSXG motif, wh ...