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- Fu, Weiqi, et al. Show all 3 Authors
- Applied microbiology and biotechnology 2007 v.75 no.4 pp. 777-782
- Escherichia coli; Rhodobacter sphaeroides; biosynthesis; codons; enzyme activity; fermenters; genes; glucose; succinic acid; temperature
- ... The 5-aminolevulinate (ALA) synthase gene (hemA) containing several codons rarely used by Escherichia coli was cloned from the genome of Rhodobacter sphaeroides and optimized in two strains of Escherichia coli: BL21(DE3) and Rosetta(DE3), which is a rare codon optimizer strain. The effects of initial isopropyl-β-D-thiogalactopyranoside (IPTG) concentration, induction time, and temperature on enzym ...