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- Xu, Qiang; Wen, Xiaopeng; Deng, Xiuxin
- Molecular breeding 2007 v.19 no.2 pp. 179-191
- Rosa roxburghii, etc ; beta-glucanase; chromosome mapping; genes; genotype; loci; phenotype; polymerase chain reaction; powdery mildew; quantitative trait loci; sequence analysis; single nucleotide polymorphism; Show all 12 Subjects
- ... Pathogenesis-related (PR) genes were isolated from chestnut rose (Rosa roxburghii Tratt) using a PCR approach with degenerate primers designed for the conserved regions of two PR gene families: class 2 (β-1,3-glucanase) and class 5 (osmotin). Thirteen PR2 and ten PR5 genes were obtained, with a nucleotide identity that ranged from 40.1 to 99.7% and from 99.2 to 99.8%, respectively. Sequence compar ...
- An, H. M.; Fan, W. G.; Chen, L. G.; Asghar, S.; Liu, Q. L.
- Journal of horticultural science & biotechnology 2007 v.82 no.4 pp. 627-635
- Rosa roxburghii, etc ; Southern blotting; amino acids; ascorbate oxidase; ascorbate peroxidase; ascorbic acid; biosynthesis; biotechnology; complementary DNA; fruiting; gene expression; genes; glucuronic acid; horticulture; messenger RNA; proteins; tissues; Show all 17 Subjects
- ... A cDNA clone encoding L-galactono-1,4-lactone dehydrogenase (GalLDH; EC 18.104.22.168) that catalyses the terminal step of ascorbic acid (AsA) biosynthesis in higher plants, was isolated from fruits of Rosa roxburghii Tratt, a plant species known to be extremely rich in AsA. The gene (RrGalLDH) encodes a protein of 589 amino acids, and the deduced amino acid was highly homologous to GalLDH proteins f ...