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You searched for: Publication year rev 7994-2006 Remove constraint Publication year rev: 7994-2006 Subject term clones Remove constraint Subject term: clones
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1. A BAC clone-based physical map of ovine major histocompatibility complex

Author:
Liu, H.; Liu, K.; Wang, J.; Ma, R.Z.
Source:
Genomics 2006 v.88 no.1 pp. 88-95
ISSN:
0888-7543
Subject:
sheep; physical chromosome mapping; major histocompatibility complex; clones; bacterial artificial chromosomes; DNA libraries
DOI:
10.1016/j.ygeno.2006.02.006
PubMed:
16595171

2. A Full-Length cDNA Infectious Clone of North American Type 1 Porcine Reproductive and Respiratory Syndrome Virus: Expression of Green Fluorescent Protein in the Nsp2 Region

Author:
Fang, Ying; Rowland, Raymond R.R.; Roof, Michael; Lunney, Joan K.; Christopher-Hennings, Jane; Nelson, Eric A.
Source:
Journal of virology 2006 v.80 no.23 pp. 11447-11455
ISSN:
1098-5514
Subject:
porcine reproductive and respiratory syndrome; green fluorescent protein; Porcine reproductive and respiratory syndrome virus; recombinant DNA; complementary DNA; antibody formation; recombinant fusion proteins; fluorescence; clones; gene expression; pathogenicity; viral proteins; genotype; reporter genes; swine
Abstract:
... The recent emergence of a unique group of North American type 1 porcine reproductive and respiratory syndrome virus (PRRSV) in the United States presents new disease control problems for a swine industry that has already been impacted seriously by North American type 2 PRRSV. In this study, a full-length cDNA infectious clone was generated from a low-virulence North American type 1 PRRSV isolate, ...
Handle:
10113/31656
DOI:
10.1128/JVI.01032-06
PubMed:
16971421
PubMed Central:
PMC1642622

3. A Statistical Test of the Hypothesis that Polyclonal Intestinal Tumors Arise by Random Collision of Initiated Clones

Author:
Michael A. Newton; Linda Clipson; Andrew T. Thliveris; Richard B. Halberg
Source:
Biometrics 2006 v.62 no.3 pp. 721-727
ISSN:
0006-341X
Subject:
Bayesian theory; biometry; clones; mice; neoplasms; statistical models
Abstract:
... The random collision hypothesis is a mathematical idealization of intestinal tumor formation that can account for the polyclonal origin of tumors without requiring a mechanistic description of clonal interaction. Using data from recent polyclonality studies in mice, we develop a statistical procedure to test the random collision hypothesis. Elements from stochastic geometry and approximations due ...
DOI:
10.1111/j.1541-0420.2006.00522.x

4. A branched-chain amino acid aminotransferase gene isolated from Hordeum vulgare is differentially regulated by drought stress

Author:
Malatrasi, M.; Corradi, M.; Svensson, J.T.; Close, T.J.; Gulli, M.; Marmiroli, N.
Source:
Theoretical and applied genetics 2006 v.113 no.6 pp. 965-976
ISSN:
0040-5752
Subject:
Arabidopsis; barley; bacterial artificial chromosomes; Hordeum vulgare; complementary DNA; seedlings; yeasts; Saccharomyces cerevisiae; clones; drought; amino acids; introns; cDNA libraries; water stress; toxicity; abscisic acid; gene expression regulation; cold; shoots
Abstract:
... Differential display was used to isolate cDNA clones showing differential expression in response to ABA, drought and cold in barley seedling shoots. One drought-regulated cDNA clone (DD12) was further analyzed and found to encode a branched-chain amino acid aminotransferase (HvBCAT-1). A genomic clone was isolated by probing the Morex BAC library with the cDNA clone DD12 and the structure of Hvbca ...
DOI:
10.1007/s00122-006-0339-6
PubMed:
16932885

5. A cDNA macroarray approach to parasite-induced gene expression changes in a songbird host: genetic response of house finches to experimental infection by Mycoplasma gallisepticum

Author:
Wang, Zhenshan; Farmer, Kristy; Hill, Geoffrey E.; Edwards, Scott V.
Source:
Molecular ecology 2006 v.15 no.5 pp. 1263-1273
ISSN:
0962-1083
Subject:
Fringillidae; Mycoplasma gallisepticum; Northern blotting; cDNA libraries; clones; complementary DNA; databases; gene expression regulation; genes; heat shock proteins; host range; hosts; immunoglobulins; mucins; mycoplasmosis; natural selection; parasites; population dynamics; songbirds; spleen; suppression subtractive hybridization; United States
Abstract:
... In 1994, the bacterial parasite Mycoplasma gallisepticum expanded its host range and swept through populations of a novel host -- eastern US populations of the house finch (Carpodacus mexicanus). This epizootic caused a dramatic decline in finch population numbers, has been shown to have caused strong selection on house finch morphology, and presumably caused evolutionary change at the molecular l ...
DOI:
10.1111/j.1365-294X.2005.02753.x
PubMed:
16626453

6. A cluster of 11 CBF transcription factors is located at the frost tolerance locus Fr-A m 2 in Triticum monococcum

Author:
Andrea K. Miller; Gabor Galiba; Jorge Dubcovsky
Source:
Molecular genetics and genomics 2006 v.275 no.2 pp. 193-203
ISSN:
1617-4615
Subject:
Triticum monococcum; bacterial artificial chromosomes; barley; breeding; clones; cold; diploidy; frost resistance; genes; loci; quantitative trait loci; temperature; transcription factors; winter; winter wheat
Abstract:
... Due to the adverse effects of cold temperatures on winter wheat, frost tolerance is an important trait for breeding programs in regions with severe winters. Frost tolerance locus Fr-A ᵐ 2 was recently discovered in diploid wheat (Triticum monococcum L.). This locus was mapped as a QTL on chromosome 5Aᵐ in the same region as a QTL for the level of transcription of the cold-regulated gene COR14b at ...
DOI:
10.1007/s00438-005-0076-6
PubMed:
16362370

7. A histidine kinase sensor protein gene is necessary for induction of low pH tolerance in Sinorhizobium sp. strain BL3

Author:
Panlada Tittabutr; Waraporn Payakapong; Neung Teaumroong; Nantakorn Boonkerd; Paul W. Singleton; Dulal Borthakur
Source:
Antonie van Leeuwenhoek 2006 v.89 no.1 pp. 125-134
ISSN:
0003-6072
Subject:
Macroptilium lathyroides; Sinorhizobium; acid tolerance; clones; environmental factors; genes; histidine kinase; mutagenesis; mutants; pH; plasmids; proteins; seedlings; sequence analysis
Abstract:
... The aim of this investigation was to identify and isolate genes involved in acid tolerance from Sinorhizobium sp. strain BL3. It was hypothesized that acid tolerance of strain BL3 could be enhanced by high level expression of certain genes involved in acid tolerance, following insertion of these genes in a multiple copy plasmid. A cosmid clone library of BL3 was introduced into BL3, and the transc ...
DOI:
10.1007/s10482-005-9015-0
PubMed:
16341462

8. A human-horse comparative map based on equine BAC end sequences

Author:
Leeb, T.; Vogl, C.; Zhu, B.; De Jong, P.J.; Binns, M.M.; Chowdhary, B.P.; Scharfe, M.; Jarek, M.; Nordsiek, G.; Schrader, F.
Source:
Genomics 2006 v.87 no.6 pp. 772-776
ISSN:
0888-7543
Subject:
bacterial artificial chromosomes; humans; horses; clones; genes; linkage (genetics); physical chromosome mapping; genetic markers; prediction
Abstract:
... In an effort to increase the density of sequence-based markers for the horse genome we generated 9473 BAC end sequences (BESs) from the CHORI-241 BAC library with an average read length of 677 bp. BLASTN searches with the BESs revealed 4036 meaningful hits (E ≤ 10−5) in the human genome that provide useful markers for the human–horse comparative map. The 4036 BLASTN hits allowed the anchoring of 3 ...
DOI:
10.1016/j.ygeno.2006.03.002
PubMed:
16603334

9. A modeling approach of the in vitro conversion of oil palm (Elaeis guineensis) somatic embryos

Author:
Eugéne E. Konan; Tristan Durand-Gasselin; Justin Y. Kouadio; Albert Flori; Alain Rival
Source:
Plant cell, tissue, and organ culture 2006 v.84 no.1 pp. 100-113
ISSN:
0167-6857
Subject:
somatic embryos; mathematical models; in vitro culture; cell growth; Elaeis guineensis; equations; clones; somatic embryogenesis; growth models; oilseed crops; micropropagation; genetic variation; in vitro regeneration; culture media; shoots
Abstract:
... A mathematical model for the growth and conversion of somatic embryos was developed with the aim of monitoring the large scale production of oil palm microplants. The predicted biomass of somatic embryos obtained and subcultured (B ₙ), together with the number of harvested shoots (Sh ₙ) – two key parameters for production forecasts – have been modeled for seven different shoot harvesting procedure ...
DOI:
10.1007/s11240-005-9010-1

10. A mutation in Arabidopsis cytochrome b5 reductase identified by high-throughput screening differentially affects hydroxylation and desaturation

Author:
Kumar, Rajesh; Wallis, James G.; Skidmore, Chris; Browse, John
Source:
plant journal 2006 v.48 no.6 pp. 920-932
ISSN:
0960-7412
Subject:
Arabidopsis; castor beans; clones; crop production; enzymes; genetic techniques and protocols; hydroxy fatty acids; hydroxylation; leaves; models; mutagenesis; screening; seeds; transgenes; yeasts
Abstract:
... As a model for analyzing the production of novel fatty acids in oilseeds, we used the genetic and molecular techniques available for Arabidopsis to characterize modifying mutations affecting the accumulation of hydroxy fatty acids in the seeds of Arabidopsis plants that express a transgene for the castor bean fatty acid hydroxylase, FAH12. We developed a high-throughput analytical system and used ...
DOI:
10.1111/j.1365-313X.2006.02925.x
PubMed:
17227547

11. A novel system for large-scale gene expression analysis: bacterial colonies array

Author:
C. Barsalobres-Cavallari; V. De Rosa Júnior; F. Nogueira; J. Ferro; S. Di Mauro; M. Menossi; E. Ulian; M. Silva-Filho
Source:
Applied microbiology and biotechnology 2006 v.71 no.6 pp. 963-969
ISSN:
0175-7598
Subject:
analysis of variance; beta-lactamase; expressed sequence tags; clones; gene expression; plasmids; microbial growth; nylon; gene expression regulation; bacteria; Escherichia coli
Abstract:
... In the present work, we report the use of bacterial colonies to optimize macroarray technique. The devised system is significantly cheaper than other methods available to detect large-scale differential gene expression. Recombinant Escherichia coli clones containing plasmid-encoded copies of 4,608 individual expressed sequence tag (ESTs) were robotically spotted onto nylon membranes that were incu ...
DOI:
10.1007/s00253-006-0348-z
PubMed:
16538485

12. A pathogenesis related protein, AhPR10 from peanut: an insight of its mode of antifungal activity

Author:
Chadha, Pooja; Das, Rakha H.
Source:
Planta 2006 v.225 no.1 pp. 213-222
ISSN:
0032-0935
Subject:
Arachis hypogaea; Escherichia coli; Fusarium oxysporum; Thanatephorus cucumeris; amino acid sequences; antifungal properties; cDNA libraries; clones; complementary DNA; energy; hyphae; microbial growth; nucleotide sequences; pathogenesis; pathogens; peanuts; proteins; ribonucleases; temperature
Abstract:
... A pathogenesis related protein (AhPR10) is identified from a clone of 6-day old Arachis hypogaea L. (peanut) cDNA library. The clone expressed as a ~20 kDa protein in E. coli. Nucleotide sequence derived amino acid sequence of the coding region shows its homology with PR10 proteins having Betv1 domain and P loop motif. Recombinant AhPR10 has ribonuclease activity, and antifungal activity against t ...
DOI:
10.1007/s00425-006-0344-7
PubMed:
16832688

13. A protein phosphatase 2A from Fagus sylvatica is regulated by GA₃ and okadaic acid in seeds and related to the transition from dormancy to germination

Author:
González-García, Mary Paz; Rodríguez, Dolores; Nicolás, Carlos; Nicolás, Gregorio; Lorenzo, Oscar
Source:
Physiologia plantarum 2006 v.128 no.1 pp. 153-162
ISSN:
0031-9317
Subject:
paclobutrazol; serine; dormancy breaking; seed stratification; gibberellic acid; clones; dormancy; Arabidopsis; seeds; complementary DNA; phosphoprotein phosphatase; protein subunits; okadaic acid; cDNA libraries; Fagus sylvatica; tobacco; seed germination; green fluorescent protein; biosynthesis; reverse transcriptase polymerase chain reaction; abscisic acid
Abstract:
... Several gibberellic acid (GA₃)-induced cDNA fragments encoding putative serine/threonine protein phosphatase (PP) 2A catalytic subunits were obtained by means of differential reverse transcriptase-PCR approach. The full-length clone, named FsPP2A1, isolated from a beechnut cDNA library, exhibited all the features of and homology to members of the PP2A family. By transient expression of FsPP2A1 in ...
DOI:
10.1111/j.1399-3054.2006.00710.x

14. A revised model for spermatogonial expansion in man: lessons from non-human primates

Author:
Ehmcke, Jens; Schlatt, Stefan
Source:
Reproduction 2006 v.132 no.5 pp. 673-680
ISSN:
1470-1626
Subject:
Primates; clones; germ cells; models
Abstract:
... We have recently described a revised scheme for spermatogonial expansion in non-human primates. We proposed that Apale-spermatogonia act as self-renewing progenitors and premeiotic germ cells are organized and divide as small clones. Here, we are revisiting the model described for man and propose a modified scheme for spermatogonial expansion. Our revised model shows high similarity to the scheme ...
DOI:
10.1530/rep.1.01081
PubMed:
17071768

15. A secondary metabolite biosynthesis cluster in Trichoderma virens: evidence from analysis of genes underexpressed in a mutant defective in morphogenesis and antibiotic production

Author:
Mukherjee, Mala; Horwitz, Benjamin A.; Sherkhane, Pramod D.; Hadar, Ruthi; Mukherjee, Prasun K.
Source:
Current genetics 2006 v.50 no.3 pp. 193-202
ISSN:
0172-8083
Subject:
heat shock proteins; biosynthesis; antibiotics; Trichoderma virens; morphogenesis; clones; cytochrome P-450; gene expression; secondary metabolites; genes; hydrophobins; mutants; fungi
Abstract:
... A transcriptional comparison of wild type and a secondary metabolite deficient Trichoderma virens mutant resulted in the identification of six genes similar to those involved in secondary metabolism in other fungi, including four cytochrome P450 genes, one O-methyl transferase and one terpene cylase. Four of the genes (three cytochrome P450s and the cyclase) are located as a cluster. Transcript le ...
DOI:
10.1007/s00294-006-0075-0
PubMed:
16804721

16. A sunflower BAC library suitable for PCR screening and physical mapping of targeted genomic regions

Author:
Bouzidi, M.F.; Franchel, J.; Tao, Q.; Stormo, K.; Mraz, A.; Nicolas, P.; Mouzeyar, S.
Source:
Theoretical and applied genetics 2006 v.113 no.1 pp. 81-89
ISSN:
0040-5752
Subject:
Helianthus annuus; DNA libraries; bacterial artificial chromosomes; clones; polymerase chain reaction; physical chromosome mapping; microsatellite repeats; genetic markers
Abstract:
... A sunflower BAC library consisting of 147,456 clones with an average size of 118 kb has been constructed and characterized. It represents approximately 5x sunflower haploid genome equivalents. The BAC library has been arranged in pools and superpools of DNA allowing screening with various PCR-based markers. Each of the 32 superpools contains 4,608 clones and corresponds to a 36 matrix pools. Thus, ...
DOI:
10.1007/s00122-006-0274-6
PubMed:
16783592

17. AFLP Analysis of the Phenetic Organization and Genetic Diversity in the Sugarcane Complex, Saccharum and Erianthus

Author:
A. Selvi; N. V. Nair; J. L. Noyer; N. K. Singh; N. Balasundaram; K. C. Bansal; K. R. Koundal; T. Mohapatra
Source:
Genetic resources and crop evolution 2006 v.53 no.4 pp. 831-842
ISSN:
0925-9864
Subject:
Saccharum; amplified fragment length polymorphism; breeding programs; clones; cluster analysis; genetic markers; genetic similarity; genetic variation; hybrids; phylogeny; random amplified polymorphic DNA technique; restriction fragment length polymorphism; sugarcane; taxonomy
Abstract:
... Amplified fragment length polymorphism (AFLP) markers were evaluated for determining the phylogenetic relationships, and the diversity in the Saccharum complex using 30 clones belonging to S. officinarum, S. robustum, S. spontaneum, S. barberi, S. sinense and the related genus Erianthus. The phenetic tree of the species clones based on AFLP data was consistent with the known taxonomical relationsh ...
DOI:
10.1007/s10722-004-6376-6

18. Aborted fruits of Opuntia microdasys (Cactaceae): insurance against reproductive failure

Author:
Palleiro, N.; Mandujano, M. C.; Golubov, J.
Source:
American journal of botany 2006 v.93 no.4 pp. 505-511
ISSN:
0002-9122
Subject:
Opuntia; soil water content; fecundity; asexual reproduction; light intensity; clones; plant density; sexual reproduction; xerophytes; plant reproduction; natural selection; genotype; seed productivity; abortion (plants); Mexico
Abstract:
... New individuals in clonal populations arise through the recruitment of sexual or clonal offspring. The predominance of one type of regeneration over the other has been correlated with different selective environmental pressures. We compared the reproductive mode (sexual through seeds and vegetative through plantlets or detached cladodes) of Opuntia microdasys from three desert habitats of the Chih ...
DOI:
10.3732/ajb.93.4.505
PubMed:
21646210

19. Abundance and Population Structure of Ammonia-Oxidizing Bacteria That Inhabit Canal Sediments Receiving Effluents from Municipal Wastewater Treatment Plants

Author:
Urakawa, Hidetoshi; Maki, Hideaki; Kawabata, Sumiko; Fujiwara, Taketomo; Ando, Haruo; Kawai, Toshio; Hiwatari, Takehiko; Kohata, Kunio; Watanabe, Masataka
Source:
Applied and environmental microbiology 2006 v.72 no.10 pp. 6845-6850
ISSN:
0099-2240
Subject:
Nitrosomonas; bacteria; clones; effluents; fluorescent antibody technique; polymerase chain reaction; population structure; restriction mapping; sediments; wastewater; wastewater treatment
Abstract:
... A polyphasic, culture-independent study was conducted to investigate the abundance and population structure of ammonia-oxidizing bacteria (AOB) in canal sediments receiving wastewater discharge. The abundance of AOB ranged from 0.2 to 1.9% and 1.6 to 5.7% of the total bacterial fraction by real-time PCR and immunofluorescence staining, respectively. Clone analysis and restriction endonuclease anal ...
DOI:
10.1128/AEM.00807-06
PubMed:
17021242
PubMed Central:
PMC1610321

20. Accuracy and Reliability of High-Throughput Microsatellite Genotyping for Cacao Clone Identification

Author:
Dapeng Zhang; Sue Mischke; Ricardo Goenaga; Alaa A. Hemeida; James A. Saunders
Source:
Crop science 2006 v.46 no.5 pp. 2084-2092
ISSN:
0011-183X
Subject:
microsatellite repeats; alleles; loci; polymerase chain reaction; Theobroma cacao; clones; heterozygosity; repeatability; genetic variation; DNA fingerprinting; accuracy; genetic markers; cultivar identification
Abstract:
... Microsatellite-based DNA fingerprinting has been increasingly applied to cacao (Theobroma cacao L.) genotype identification. However, the accuracy and reliability of using high throughput microsatellite analysis for cacao clone identification have not yet been rigorously assessed. Despite the use of highly robust fingerprinting protocols, cacao genotype identification has been affected by genotypi ...
Handle:
10113/3413
DOI:
10.2135/cropsci2006.01.0004

21. Achieving higher heritabilities through improved design and analysis of clonal trials

Author:
Gezan, S.A.; White, T.L.; Huber, D.A.
Source:
Canadian journal of forest research = 2006 v.36 no.9 pp. 2148-2156
ISSN:
0045-5067
Subject:
simulation models; tree breeding; forest trees; equations; spatial distribution; row spacing; clones; statistical analysis; heritability
Abstract:
... Clonal testing was studied under different environmental patterns and experimental designs through simulation with the criteria of maximizing broad-sense heritability estimates and genetic gain from clonal selection. Several experimental designs were studied together with three patterns of environmental variability. In addition, empirical 95% confidence intervals for heritability estimates were co ...
DOI:
10.1139/X06-113

22. Advanced resources for plant genomics: a BAC library specific for the short arm of wheat chromosome 1B

Author:
Janda, Jaroslav; Šafář, Jan; Kubaláková, Marie; Bartoš, Jan; Kovářová, Pavlína; Suchánková, Pavla; Pateyron, Stephanie; Číhalíková, Jarmila; Sourdille, Pierre; Šimková, Hana; Faivre-Rampant, Patricia; Hřibová, Eva; Bernard, Michel; Lukaszewski, Adam; Doležel, Jaroslav; Chalhoub, Boulos
Source:
plant journal 2006 v.47 no.6 pp. 977-986
ISSN:
0960-7412
Subject:
DNA; Triticum aestivum; bacterial artificial chromosomes; clones; crops; genome; genomics; molecular cloning; probability; sorting; wheat
Abstract:
... Common wheat (Triticum aestivum L., 2n = 6x = 42) is a polyploid species possessing one of the largest genomes among the cultivated crops (1C is approximately 17 000 Mb). The presence of three homoeologous genomes (A, B and D), and the prevalence of repetitive DNA make sequencing the wheat genome a daunting task. We have developed a novel 'chromosome arm-based' strategy for wheat genome sequencing ...
DOI:
10.1111/j.1365-313X.2006.02840.x
PubMed:
16911585

23. Advantage of Using Inosine at the 3' Termini of 16S rRNA Gene Universal Primers for the Study of Microbial Diversity

Author:
Ben-Dov, Eitan; Shapiro, Orr H.; Siboni, Nachshon; Kushmaro, Ariel
Source:
Applied and environmental microbiology 2006 v.72 no.11 pp. 6902-6906
ISSN:
0099-2240
Subject:
Firmicutes; Proteobacteria; annealing; clones; genes; microbial communities; polymerase chain reaction; ribosomal RNA; temperature
Abstract:
... To overcome the shortcomings of universal 16S rRNA gene primers 8F and 907R when studying the diversity of complex microbial communities, the 3' termini of both primers were replaced with inosine. A comparison of the clone libraries derived using both primer sets showed seven bacterial phyla amplified by the altered primer set (8F-I/907R-I) whereas the original set amplified sequences belonging al ...
DOI:
10.1128/AEM.00849-06
PubMed:
16950904
PubMed Central:
PMC1636166

24. Age-Related Shifts in Leaf Chemistry of Clonal Aspen (Populus tremuloides)

Author:
Donaldson, J.R.; Stevens, M.T.; Barnhill, H.R.; Lindroth, R.L.
Source:
Journal of chemical ecology 2006 v.32 no.7 pp. 1415-1429
ISSN:
0098-0331
Subject:
proanthocyanidins; developmental stages; plant-insect relations; herbivores; clones; plant age
Abstract:
... Developmental changes in plant structure and function can influence both mammalian and arthropod feeding preferences for many woody plant species. This study documents age-related changes that occur in the leaf chemistry of trembling aspen (Populus tremuloides Michx., Salicaceae) and discusses implications for the herbivore community and ecosystem processes. We collected leaves from replicate rame ...
DOI:
10.1007/s10886-006-9059-2
PubMed:
16724272

25. Agroinfection-based high-throughput screening reveals specific recognition of INF elicitins in Solanum

Author:
VLEESHOUWERS, VIVIANNE G.A.A.; DRIESPRONG, JAN-DAVID; KAMPHUIS, LARS G.; TORTO-ALALIBO, TRUDY; SLOT, KLAAS A.E. VAN'T; GOVERS, FRANCINE; VISSER, RICHARD G.F.; JACOBSEN, EVERT; KAMOUN, SOPHIEN
Source:
Molecular plant pathology 2006 v.7 no.6 pp. 499-510
ISSN:
1464-6722
Subject:
cell death; Nicotiana; Agrobacterium radiobacter; Solanum huancabambense; plant pathogens; Phytophthora infestans; clones; Solanum microdontum; potatoes; disease resistance; screening
Abstract:
... We adapted and optimized the use of the Agrobacterium tumefaciens binary PVX expression system (PVX agroinfection) to screen Solanum plants for response to pathogen elicitors and applied the assay to identify a total of 11 clones of Solanum huancabambense and Solanum microdontum, out of 31 species tested, that respond to the elicitins INF1, INF2A and INF2B of Phytophthora infestans. Prior to this ...
DOI:
10.1111/j.1364-3703.2006.00355.x
PubMed:
20507464

26. Allelic Size Standards and Reference Genotypes to Unify International Cocoa (Theobroma cacao L.) Microsatellite Data

Author:
Cryer, N. C.; Fenn, M. G. E.; Turnbull, C. J.; Wilkinson, M. J.
Source:
Genetic resources and crop evolution 2006 v.53 no.8 pp. 1643-1652
ISSN:
0925-9864
Subject:
germplasm; microsatellite repeats; alleles; Theobroma cacao; clones; genotype; gene banks; DNA fingerprinting; cultivar identification; genetic markers
Abstract:
... Standardisation of microsatellite allele profiles between laboratories is of fundamental importance to the transferability of genetic fingerprint data and the identification of clonal individuals held at multiple sites. Here we describe two methods of standardisation applied to the microsatellite fingerprinting of 429 Theobroma cacao L. trees representing 345 accessions held in the worlds largest ...
DOI:
10.1007/s10722-005-1286-9

27. An Anaerobic Methane-Oxidizing Community of ANME-1b Archaea in Hypersaline Gulf of Mexico Sediments

Author:
Lloyd, Karen G.; Lapham, Laura; Teske, Andreas
Source:
Applied and environmental microbiology 2006 v.72 no.11 pp. 7218-7230
ISSN:
0099-2240
Subject:
Archaea; DNA libraries; DNA primers; canyons; clones; community structure; electrons; genes; methane; microbial communities; molecular cloning; oxidation; polymerase chain reaction; ribosomal RNA; sediments; sulfates; Gulf of Mexico
Abstract:
... Sediments overlying a brine pool methane seep in the Gulf of Mexico (Green Canyon 205) were analyzed using molecular and geochemical approaches to identify geochemical controls on microbial community composition and stratification. 16S rRNA gene and rRNA clone libraries, as well as mcrA gene clone libraries, showed that the archaeal community consists predominantly of ANME-1b methane oxidizers; no ...
DOI:
10.1128/AEM.00886-06
PubMed:
16980428
PubMed Central:
PMC1636178

28. An Integrated Approach to Functional Genomics: Construction of a Novel Reporter Gene Fusion Library for Sinorhizobium meliloti

Author:
Cowie, Alison; Cheng, Jiujun; Sibley, Christopher D.; Fong, Ying; Zaheer, Rahat; Patten, Cheryl L.; Morton, Richard M.; Golding, G. Brian; Finan, Turlough M.
Source:
Applied and environmental microbiology 2006 v.72 no.11 pp. 7156-7167
ISSN:
0099-2240
Subject:
Internet; gene deletion; glucose; clones; gene expression; databases; Escherichia coli; sequence analysis; messenger RNA; DNA libraries; succinic acid; transcription (genetics); enzyme activity; plasmid vectors; Ensifer meliloti; homologous recombination; gene targeting; operon; gene fusion; genomics; nucleotide sequences; reporter genes; bacteria
Abstract:
... As a means of investigating gene function, we developed a robust transcription fusion reporter vector to measure gene expression in bacteria. The vector, pTH1522, was used to construct a random insert library for the Sinorhizobium meliloti genome. pTH1522 replicates in Escherichia coli and can be transferred to, but cannot replicate in, S. meliloti. Homologous recombination of the DNA fragments cl ...
DOI:
10.1128/AEM.01397-06
PubMed:
16963549
PubMed Central:
PMC1636157

29. An SSR-based linkage map of Capsicum annuum

Author:
Minamiyama, Y.; Tsuro, M.; Hirai, M.
Source:
Molecular breeding 2006 v.18 no.2 pp. 157-169
ISSN:
1380-3743
Subject:
Capsicum annuum; peppers; genetic markers; microsatellite repeats; clones; chromosome mapping
Abstract:
... There are five cultivated species of pepper, of which Capsicum annuum is the most widely cultivated as a vegetable or spice and the main experimental material of most pepper breeding programs. However, the number of simple sequence-repeat (SSR) markers known for C. annuum is limited. To develop SSR markers for Capsicum species, we constructed four SSR-enriched libraries from the genomic DNA of C. ...
DOI:
10.1007/s11032-006-9024-3

30. An eIF4E allele confers resistance to an uncapped and non-polyadenylated RNA virus in melon

Author:
Nieto, Cristina; Morales, Monica; Orjeda, Gisella; Clepet, Christian; Monfort, Amparo; Sturbois, Benedicte; Puigdomènech, Pere; Pitrat, Michel; Caboche, Michel; Dogimont, Catherine; Garcia-Mas, Jordi; Aranda, Miguel.A.; Bendahmane, Abdelhafid
Source:
plant journal 2006 v.48 no.3 pp. 452-462
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Melon necrotic spot virus; Nicotiana benthamiana; Potyviridae; RNA; alleles; amino acids; bacterial artificial chromosomes; clones; melons; mutants; protein synthesis; recessive genes; viruses
Abstract:
... The characterization of natural recessive resistance genes and virus-resistant mutants of Arabidopsis have implicated translation initiation factors of the 4E family [eIF4E and eIF(iso)4E] as susceptibility factors required for virus multiplication and resistance expression. To date, viruses controlled by these genes mainly belong to the family Potyviridae. Melon necrotic spot virus (MNSV) belongs ...
DOI:
10.1111/j.1365-313X.2006.02885.x
PubMed:
17026540

31. An integrated map of Oryza sativa L. chromosome 5

Author:
Fang-I Kao; Yueh-Yun Cheng; Teh-Yuan Chow; Hong-Hwa Chen; Shu-Mei Liu; Chia-Hsiung Cheng; Mei-Chu Chung
Source:
Theoretical and applied genetics 2006 v.112 no.5 pp. 891-902
ISSN:
0040-5752
Subject:
restriction fragment length polymorphism; fluorescence in situ hybridization; rice; DNA libraries; bacterial artificial chromosomes; Oryza sativa; clones; physical chromosome mapping
Abstract:
... The developments of molecular marker-based genetic linkage maps are now routine. Physical maps based on contigs of large insert genomic clones have been established in several plant species. However, integration of genetic, physical, and cytological maps is still a challenge for most plant species. Here we present an integrated map of rice (Oryza sativa L.) chromosome 5, developed by fluorescence ...
DOI:
10.1007/s00122-005-0191-0
PubMed:
16365756

32. Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship

Author:
Dillon, R.J.; Ivens, A.C.; Churcher, C.; Holroyd, N.; Quail, M.A.; Rogers, M.E.; Soares, M.B.; Bonaldo, M.F.; Casavant, T.L.; Lehane, M.J.
Source:
Genomics 2006 v.88 no.6 pp. 831-840
ISSN:
0888-7543
Subject:
Lutzomyia longipalpis; midgut; expressed sequence tags; clones; digestion; insect physiology; cDNA libraries; immune response
DOI:
10.1016/j.ygeno.2006.06.011
PubMed:
16887324
PubMed Central:
PMC2675706

33. Analysis of Expressed Sequence Tags from the Harmful Alga, Prymnesium parvum (Prymnesiophyceae, Haptophyta)

Author:
John W. La Claire II
Source:
Marine biotechnology 2006 v.8 no.5 pp. 534-546
ISSN:
1436-2228
Subject:
Prymnesium; biochemical pathways; cDNA libraries; clones; complementary DNA; databases; expressed sequence tags; phosphates; photosynthesis; protein synthesis; proteins; secretion; toxicity; toxins; unigenes
Abstract:
... A cDNA library was constructed from late log-phase cultures of the ichthyotoxin-producing haptophyte, Prymnesium parvum. Approximately 6,300 single-pass 5′ nucleotide sequences (out of nearly 8,000 randomly isolated clones) achieved length and quality criteria for incorporation into an expressed sequence tag (EST) database. Collectively, 3,415 unique genes (tentative unigenes [TUGs]) were assemble ...
DOI:
10.1007/s10126-005-5182-2

34. Analysis of expressed sequence tags from Cryptomeria japonica pollen reveals novel pollen-specific transcripts

Author:
Futamura, N.; Ujino-Ihara, T.; Nishiguchi, M.; Kanamori, H.; Yoshimura, K.; Sakaguchi, M.; Shinohara, K.
Source:
Tree physiology 2006 v.26 no.12 pp. 1517-1528
ISSN:
0829-318X
Subject:
messenger RNA; protein synthesis; forest trees; complementary DNA; Cryptomeria japonica; allergens; clones; gene expression; expressed sequence tags; transcription (genetics); transcriptome; amino acid sequences; pollen; poisonous plants; Japan
Abstract:
... Cryptomeria japonica D. Don is one of the most important forest trees in Japan, but more than 10% of the Japanese population is allergic to its pollen. We constructed a cDNA library derived from pollen grains of C. japonica and performed an analysis of expressed sequence tags (ESTs). We obtained partial sequences from 1929 clones, which represented 1365 unique transcripts. Among the unique transcr ...
DOI:
10.1093/treephys/26.12.1517
PubMed:
17169891

35. Analysis of genetic factors related to preferential selection of the NSP1 gene segment observed in mixed infection and multiple passage of rotaviruses

Author:
Mahbub Alam, M.; Kobayashi, N.; Ishino, M.; Naik, T. N.; Taniguchi, K.
Source:
Archives of virology 2006 v.151 no.11 pp. 2149-2159
ISSN:
0304-8608
Subject:
clones; genes; genetic background; genetic techniques and protocols; mixed infection; progeny; viruses
Abstract:
... Reassortment is one of the major evolutionary mechanisms of the rotavirus genome. Preferential selection (assortment) of the NSP1 gene segment from either of the parental viruses after coinfection of these viruses has been reported as a notable finding in reassortment. To analyze genetic factors which are associated with preferential selection of the rotavirus NSP1 gene segment into progeny viruse ...
DOI:
10.1007/s00705-006-0795-y
PubMed:
16773237

36. Analysis of the gacS-gacA regulatory genes of spontaneous mutants of Pseudomonas fluorescens biocontrol strain PfG32R

Author:
W. Gusti Ngurah Alit-Susanta; Yuichi Takikawa
Source:
Journal of general plant pathology 2006 v.72 no.3 pp. 159-167
ISSN:
1345-2630
Subject:
Clavibacter michiganensis subsp. michiganensis; Pseudomonas fluorescens; Ralstonia solanacearum; amino acids; antibacterial properties; antifungal properties; biological control; biological control agents; clones; mutants; mutation; pathogens; proteinases; regulator genes; sequence analysis; siderophores
Abstract:
... Pseudomonas fluorescens strain PfG32R, a potential biocontrol agent against soilborne pathogens, frequently loses its antifungal activity and ability to produce enzymes. To characterize genetically the instability of these bacterial functions, we analyzed gacS and gacA genes of PfG32R and three spontaneous mutants of PfG32R (NR1, NR9, and ASW6), which had lost their ability to produce proteases an ...
DOI:
10.1007/s10327-005-0271-7

37. Anchoring of a large set of markers onto a BAC library for the development of a draft physical map of the grapevine genome

Author:
Lamoureux, D.; Bernole, A.; Le Clainche, I.; Tual, S.; Thareau, V.; Paillard, S.; Legeai, F.; Dossat, C.; Wincker, P.; Oswald, M.
Source:
Theoretical and applied genetics 2006 v.113 no.2 pp. 344-356
ISSN:
0040-5752
Subject:
microsatellite repeats; DNA libraries; bacterial artificial chromosomes; wine grapes; polymerase chain reaction; expressed sequence tags; clones; Vitis vinifera; wine cultivars; physical chromosome mapping; genetic markers
Abstract:
... Five hundred and six EST-derived markers, 313 SSR markers and 26 BAC end-derived or SCAR markers were anchored by PCR on a subset of a Cabernet Sauvignon BAC library representing six genome equivalents pooled in three dimensions. In parallel, the 12,351 EST clusters of the grapevine UniGene set (build #11) from NCBI were used to design 12,125 primers pairs and perform electronic PCR on 67,543 nonr ...
DOI:
10.1007/s00122-006-0301-7
PubMed:
16791700

38. Annotation and expression profile analysis of 2073 full-length cDNAs from stress-induced maize (Zea mays L.) seedlings

Author:
Jia, Jinping; Fu, Junjie; Zheng, Jun; Zhou, Xin; Huai, Junling; Wang, Jianhua; Wang, Meng; Zhang, Ying; Chen, Xiaoping; Zhang, Jinpeng; Zhao, Jinfeng; Su, Zhen; Lv, Yuping; Wang, Guoying
Source:
plant journal 2006 v.48 no.5 pp. 710-727
ISSN:
0960-7412
Subject:
Zea mays; bacterial artificial chromosomes; cDNA libraries; clones; complementary DNA; corn; databases; ethylene; genes; messenger RNA; plant response; promoter regions; seedlings; stress response; transcription factors
Abstract:
... Full-length cDNAs are very important for genome annotation and functional analysis of genes. The number of full-length cDNAs from maize (Zea mays L.) remains limited. Here we report the construction of a full-length enriched cDNA library from osmotically stressed maize seedlings by using the modified CAP trapper method. From this library, 2073 full-length cDNAs (accession numbers DQ244142- DQ24621 ...
DOI:
10.1111/j.1365-313X.2006.02905.x
PubMed:
17076806

39. Anopheles gambiae genome reannotation through synthesis of ab initio and comparative gene prediction algorithms

Author:
Jun Li; Michelle M Riehle; Yan Zhang; Jiannong Xu; Frederick Oduol; Shawn M Gomez; Karin Eiglmeier; Beatrix M Ueberheide; Jeffrey Shabanowitz; Donald F Hunt; José MC Ribeiro; Kenneth D Vernick
Source:
Genome biology 2006 v.7 no.3 pp. 1292
ISSN:
1474-760X
Subject:
stop codon; complementary DNA; bioinformatics; data collection; expressed sequence tags; clones; insect vectors; mass spectrometry; proteomics; Anopheles gambiae; malaria; algorithms; databases; researchers; prediction
Abstract:
... BACKGROUND: Complete genome annotation is a necessary tool as Anopheles gambiae researchers probe the biology of this potent malaria vector. RESULTS: We reannotate the A. gambiae genome by synthesizing comparative and ab initio sets of predicted coding sequences (CDSs) into a single set using an exon-gene-union algorithm followed by an open-reading-frame-selection algorithm. The reannotation predi ...
DOI:
10.1186/gb-2006-7-3-r24
PubMed:
16569258
PubMed Central:
PMC1557760

40. Antigenic Variation of TprK V Regions Abrogates Specific Antibody Binding in Syphilis

Author:
LaFond, Rebecca E.; Molini, Barbara J.; Van Voorhis, Wesley C.; Lukehart, Sheila A.
Source:
Infection and immunity 2006 v.74 no.11 pp. 6244-6251
ISSN:
0019-9567
Subject:
Treponema pallidum; antibodies; antigenic variation; clones; cross reaction; genes; immunoassays; rabbits; synthetic peptides
Abstract:
... The tprK gene in the syphilis spirochete, Treponema pallidum subsp. pallidum, undergoes antigenic variation in seven variable (V) regions. tprK is highly variable within T. pallidum strains, and a method has been developed to derive clones of T. pallidum that express a single, unique tprK sequence. Rabbits were infected with three different T. pallidum clones or the parent strain from which the cl ...
DOI:
10.1128/IAI.00827-06
PubMed:
16923793
PubMed Central:
PMC1695500

41. Antioxidant capacity of crude extracts from clones of banana and plane species

Author:
Perez-Perez, E.M.; Rodriguez-Malaver, A.J.; Padilla, N.; Medina-Ramirez, G.; Davila, J.
Source:
Journal of medicinal food 2006 v.9 no.4 pp. 517-523
ISSN:
1096-620X
Subject:
hybrids; plantains (fruit); uric acid; free radicals; lipid peroxidation; plant extracts; hydroperoxides; clones; Musa acuminata; Musa balbisiana; oxidation; interspecific hybridization; superoxide anion; antioxidant activity; ultraviolet radiation; bananas
DOI:
10.1089/jmf.2006.9.517
PubMed:
17201639

42. Artificial induction of flowering in sugarcane under sub-tropical conditions — A successful approach

Author:
R. P. Srivastava; S. P. Singh; PRATAP Singh; S. B. Singh
Source:
Sugar tech 2006 v.8 no.2-3 pp. 184-186
ISSN:
0972-1525
Subject:
Saccharum spontaneum; adverse effects; clones; crossing; flowering; flowers; genotype; pollen; solar radiation; subtropics; sugarcane; temperature
Abstract:
... Four hours extra dark treatment in artificially made photoperiodic chamber induced early flowering in flowering clones i.e. S. 55/99, S. 187/91,S. 301/86, S. 116/2K.S. 73/89 after 12to 14th weeks. Maximum and minimum temperature ranged from 31.0 and 21.4°C respectively. Control plants of respective genotypes flowered generally after one month of the treated plants. Flowered genotypes in photoperio ...
DOI:
10.1007/BF02943658

43. Associations between DNA markers and resistance to diseases in sugarcane and effects of population substructure

Author:
Wei, Xianming; Jackson, Phillip A.; McIntyre, C. Lynne; Aitken, Karen S.; Croft, Barry
Source:
Theoretical and applied genetics 2006 v.114 no.1 pp. 155-164
ISSN:
0040-5752
Subject:
Fiji disease virus; Ustilago scitaminea; Xanthomonas albilineans; amplified fragment length polymorphism; ancestry; breeding; clones; disease resistance; genetic markers; leaves; linkage disequilibrium; parents; phenotypic variation; population structure; regression analysis; root rot; sugarcane
Abstract:
... Association between markers and sugarcane diseases were investigated in a collection of 154 sugarcane clones, consisting of important ancestors or parents, and cultivars. 1,068 polymorphic AFLP and 141 SRR markers were scored across all clones. Data on the four most important diseases in the Australian sugarcane industry were obtained; these diseases being pachymetra root rot (Pachymetra chaunorhi ...
DOI:
10.1007/s00122-006-0418-8
PubMed:
17047910

44. Bacteria Associated with Mucus and Tissues of the Coral Oculina patagonica in Summer and Winter

Author:
Koren, Omry; Rosenberg, Eugene
Source:
Applied and environmental microbiology 2006 v.72 no.8 pp. 5254-5259
ISSN:
0099-2240
Subject:
DNA; Pseudomonas; Vibrio splendidus; agar; bacteria; bacterial communities; centrifugation; clones; corals; databases; genes; gold; mucus; nucleotide sequences; ribosomal RNA; summer; winter
Abstract:
... The relative abundance of bacteria in the mucus and crushed tissue of the Mediterranean coral Oculina patagonica was determined by analyses of the 16S rRNA genes of isolated colonies and from a 16S rRNA clone library of extracted DNA. By SYBR gold staining, the numbers of bacteria in mucus and tissue samples were 6.2 x 10⁷ and 8.3 x 10⁸/cm² of coral surface, respectively, 99.8% of which failed to ...
DOI:
10.1128/AEM.00554-06
PubMed:
16885273
PubMed Central:
PMC1538755

45. Biogeography and Landscape-Scale Diversity of the Dominant Crenarchaeota of Soil

Author:
Oline, David K.; Schmidt, Steven K.; Grant, Michael C.
Source:
Microbial ecology 2006 v.52 no.3 pp. 480-490
ISSN:
0095-3628
Subject:
Crenarchaeota; alpine tundra; biogeography; clones; nucleotide sequences; polymerase chain reaction; ribosomal DNA; soil; soil sampling; soil surveys; Rocky Mountain region
Abstract:
... We surveyed the diversity of soil Archaea across a large scale elevational gradient of ecosystem types, from foothills forest to alpine tundra in the Front Range of the Rocky Mountains. We used a dilution technique to sequence the single most abundant archaeal 16S rDNA sequence in each of the 40 soil cores distributed across the gradient to compare our results to those of typical 16S clone library ...
DOI:
10.1007/s00248-006-9101-5
PubMed:
16909343

46. Carbon Isotope Discrimination, Gas Exchange and Stem Growth of Four Euramerican Hybrid Poplars under Different Watering Regimes

Author:
Voltas, J.; Serrano, L.; Hernandez, M.; Peman, J.
Source:
New forests 2006 v.31 no.3 pp. 435-451
ISSN:
0169-4286
Subject:
gas exchange; drought; carbon; seasonal variation; carbon dioxide; hybrids; stomatal conductance; stems; isotopes; dry matter accumulation; clones; water stress; photosynthesis; Populus; forest trees; net assimilation rate; tree growth; water use efficiency; flood irrigation; genetic variation; Spain
Abstract:
... Seasonal changes in carbon isotope discrimination (Δ) and gas exchange traits were assessed in four Populusxeuramericana clones differing in growth potential. Measurements were made during the second year after establishment in the field under two watering regimes, which were defined by the time-span between flood irrigations, hence resulting in different dry-down cycles: high irrigation (conserva ...
DOI:
10.1007/s11056-005-0879-7

47. Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals

Author:
Ohmiya, Akemi; Kishimoto, Sanae; Aida, Ryutaro; Yoshioka, Satoshi; Sumitomo, Katsuhiko
Source:
Plant physiology 2006 v.142 no.3 pp. 1193-1201
ISSN:
0032-0889
Subject:
Chrysanthemum morifolium; RNA interference; amino acid sequences; carotenoids; clones; color; corolla; cultivars; gene expression; leaves; roots; sequence homology; stems
Abstract:
... The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clone ...
DOI:
10.1104/pp.106.087130
PubMed:
16980560
PubMed Central:
PMC1630759

48. Changes in host chitinase isoforms in relation to wounding and colonization by Heterobasidion annosum: early and strong defense response in 33-year-old resistant Norway spruce clone

Author:
Fossdal, C.G.; Hietala, A.M.; Kvaalen, H.; Solheim, H.
Source:
Tree physiology 2006 v.26 no.2 pp. 169-177
ISSN:
0829-318X
Subject:
temporal variation; forest trees; quantitative analysis; fungal diseases of plants; genetic variation; polymerase chain reaction; Heterobasidion annosum; Picea abies; clones; pathogenicity; plant pathogenic fungi; spatial variation; chemical constituents of plants; isozymes; chitinase; Norway
Abstract:
... We studied the defense reactions of 33-year-old susceptible and resistant clones of Norway spruce (Picea abies (L.) Karst.) to the major root-rot fungus Heterobasidion annosum (Fr.) Bref. and determined if tissue cultures can be used as a model system for studying defense responses of mature trees at the molecular level. Quantitative PCR analysis of genomic DNA obtained from samples taken at diffe ...
DOI:
10.1093/treephys/26.2.169
PubMed:
16356913

49. Characterization of Monoclonal Antibodies Against Fowl Adenovirus Serotype 1 (FAV1) Isolated from Gizzard Erosion

Author:
Taharaguchi, S.; Ito, H.; Ohta, H.; Takase, K.
Source:
Avian diseases 2006 v.50 no.3 pp. 331-335
ISSN:
0005-2086
Subject:
Fowl aviadenovirus A; chickens; hybridomas; disease detection; lesions (animal); gizzard; serodiagnosis; viral diseases of animals and humans; serotypes; poultry diseases; tissue analysis; clones; pathogen identification; enzyme-linked immunosorbent assay; monoclonal antibodies; viral proteins
Abstract:
... Six clones of monoclonal antibodies (Mabs) to fowl adenovirus (FAV) serotype 1 were produced. All Mabs reacted positively by enzyme-linked immunosorbent assay. Three Mabs recognized the putative 100-kD hexon protein and reacted to serotype 1 specifically by western blot analysis but did not react to other FAV serotypes (2, 3, 4, 5, 6, 7, and 8a). These Mabs will be useful for immunodiagnosis of FA ...
DOI:
10.1637/7460-102605R.1
PubMed:
17039830

50. Characterization of Novel Carbazole Catabolism Genes from Gram-Positive Carbazole Degrader Nocardioides aromaticivorans IC177

Author:
Inoue, Kengo; Habe, Hiroshi; Yamane, Hisakazu; Nojiri, Hideaki
Source:
Applied and environmental microbiology 2006 v.72 no.5 pp. 3321-3329
ISSN:
0099-2240
Subject:
Escherichia coli; Nocardioides; Pseudomonas resinovorans; Sphingomonas; acetyl coenzyme A; amino acid motifs; amino acid sequences; clones; electron transfer; iron; metabolism; multigene family; naphthalene; open reading frames; operon; pyruvic acid; reverse transcriptase polymerase chain reaction
Abstract:
... Nocardioides aromaticivorans IC177 is a gram-positive carbazole degrader. The genes encoding carbazole degradation (car genes) were cloned into a cosmid clone and sequenced partially to reveal 19 open reading frames. The car genes were clustered into the carAaCBaBbAcAd and carDFE gene clusters, encoding the enzymes responsible for the degradation of carbazole to anthranilate and 2-hydroxypenta-2,4 ...
DOI:
10.1128/AEM.72.5.3321-3329.2006
PubMed:
16672473
PubMed Central:
PMC1472339