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- Li, Huijuan, et al. Show all 18 Authors
- Science 2006 v.313 no.5792 pp. 1441-1443
- engineering; Komagataella pastoris; humans; glycosylation; recombinant proteins; yeasts; genes; erythropoietin
- ... Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate the sequential steps of human glycosylation. The rep ...