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- Author:
- Savitsky, I., et al. ; Molad, T.; Mazuz, M.L.; Fleiderovitz, L.; Fish, L.; Krigel, Y.; Leibovitz, B.; Molloy, J.; Jongejan, F.; Shkap, V.; Show all 10 Authors
- Source:
- Veterinary microbiology 2006 v.113 no.1-2 pp. 55-62
- ISSN:
- 0378-1135
- Subject:
- cattle; Anaplasma marginale; Anaplasma centrale; anaplasmosis; cattle diseases; disease detection; serodiagnosis; grazing; polymerase chain reaction; ribosomal RNA; microbial genetics; genes; antibodies; vaccination; enzyme-linked immunosorbent assay; disease outbreaks; disease control; Israel
- Abstract:
- ... A reverse line blot hybridization (RLB) one-stage nested PCR (nPCR) for Anaplasma centrale and a nested PCR for Anaplasma marginale were used to detect infected cattle grazing within an endemic region in Israel. A novel set of PCR primers and oligonucleotide probes based on a 16S ribosomal RNA gene was designed for RLB detection of both Anaplasma species, and the performance of the molecular assay ...
- DOI:
- 10.1016/j.vetmic.2005.10.026
-
http://dx.doi.org/10.1016/j.vetmic.2005.10.026