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You searched for: Subject "recombinant DNA" Remove constraint Subject: "recombinant DNA" Publication Year 2006 Remove constraint Publication Year: 2006 Subject promoter regions Remove constraint Subject: promoter regions
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1. A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients

Author:
Leimanis, S.; Hernandez, M.; Fernandez, S.; Boyer, F.; Burns, M.; Bruderer, S.; Glouden, T.; Harris, N.; Kaeppeli, O.; Philipp, P.
Source:
Plant molecular biology 2006 v.61 no.1-2 pp. 123-139
ISSN:
0167-4412
Subject:
recombinant DNA,   etc   ; microarray technology; polymerase chain reaction; transgenic plants; food crops; Cauliflower mosaic virus; promoter regions; reporter genes; kinases; Agrobacterium tumefaciens; Streptomyces hygroscopicus; kanamycin kinase;     Show all 12 Subjects
Abstract:
... A multiplex DNA microarray chip was developed for simultaneous identification of nine genetically modified organisms (GMOs), five plant species and three GMO screening elements, i.e. the 35S promoter, the nos terminator and the nptII gene. The chips also include several controls, such as that for the possible presence of CaMV. The on-chip detection was performed directly with PCR amplified product ...
DOI:
10.1007/s11103-005-6173-4

2. Developmental Expression Patterns of Arabidopsis XTH Genes Reported by Transgenes and Genevestigator

Author:
Becnel, J.; Natarajan, M.; Kipp, A.; Braam, J.
Source:
Plant molecular biology 2006 v.61 no.3 pp. 451-467
ISSN:
0167-4412
Subject:
recombinant DNA,   etc   ; Arabidopsis thaliana; genes; hexosyltransferases; O-glycoside hydrolases; promoter regions; beta-glucuronidase; genetic techniques and protocols; transgenes; transgenic plants; gene expression; cell walls; plant development; developmental stages;     Show all 14 Subjects
Abstract:
... The plant cell wall is the structural basis of cellular form and thus forms a foundation on which morphogenesis builds organs and tissues. Enzymes capable of modifying major wall components are prominent candidates for regulating wall form and function. Xyloglucan endotransglucosylases/hydrolases (XTHs) are predicted to participate in xyloglucan integration and/or restructuring. XTHs are encoded b ...
DOI:
10.1007/s11103-006-0021-z
PubMed:
16830179

3. Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction

Author:
Mandon, C.A.; Diaz-Latoud, C.; Arrigo, A.P.; Blum, L.J.
Source:
Journal of biotechnology 2006 v.124 no.2 pp. 392-402
ISSN:
0168-1656
Subject:
recombinant DNA,   etc   ; thiram; fungicide residues; cadmium; toxicity testing; bioassays; gene expression; reporter genes; luciferase; green fluorescent protein; bioluminescence; fluorescence; promoter regions; Drosophila melanogaster; liver neoplasms; heat shock proteins; uterine cervical neoplasms; cell lines; humans;     Show all 19 Subjects
DOI:
10.1016/j.jbiotec.2006.01.004

4. Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae

Author:
Nevoigt, Elke; Kohnke, Jessica; Fischer, Curt R.; Alper, Hal; Stahl, Ulf; Stephanopoulos, Gregory
Source:
Applied and environmental microbiology 2006 v.72 no.8 pp. 5266-5273
ISSN:
0099-2240
Subject:
recombinant DNA,   etc   ; Saccharomyces cerevisiae; yeasts; genetically engineered microorganisms; genetic vectors; promoter regions; genetic engineering; mutants; transcription (genetics); gene expression; enzyme activity; glycerol-3-phosphate dehydrogenase; genes;     Show all 13 Subjects
Abstract:
... The strong overexpression or complete deletion of a gene gives only limited information about its control over a certain phenotype or pathway. Gene function studies based on these methods are therefore incomplete. To effect facile manipulation of gene expression across a full continuum of possible expression levels, we recently created a library of mutant promoters. Here, we provide the detailed c ...
DOI:
10.1128/AEM.00530-06
PubMed:
16885275
PubMed Central:
PMC1538763

5. Expression of the 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Gene Requires Symbiotic Nitrogen-Fixing Regulator Gene nifA2 in Mesorhizobium loti MAFF303099

Author:
Nukui, Noriyuki; Minamisawa, Kiwamu; Ayabe, Shin-Ichi; Aoki, Toshio
Source:
Applied and environmental microbiology 2006 v.72 no.7 pp. 4964-4969
ISSN:
0099-2240
Subject:
recombinant DNA,   etc   ; Mesorhizobium loti; regulator genes; transcription factors; gene expression; transcription (genetics); amidine hydrolases; promoter regions; reporter genes; beta-glucuronidase; root nodules; nodulation; Lotus corniculatus var. japonicus;     Show all 13 Subjects
Abstract:
... Many soil bacteria contain 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which degrades ACC, a precursor of the phytohormone ethylene. In order to examine the regulation of the acdS gene encoding ACC deaminase in Mesorhizobium loti MAFF303099 during symbiosis with the host legume Lotus japonicus, we introduced the β-glucuronidase (GUS) gene into acdS so that GUS was expressed under contro ...
DOI:
10.1128/AEM.02745-05
PubMed:
16820494
PubMed Central:
PMC1489367

6. Glycosylated F4 (K88) Fimbrial Adhesin FaeG Expressed in Barley Endosperm Induces ETEC-neutralizing Antibodies in Mice

Author:
Joensuu, J.J.; Kotiaho, M.; Teeri, T.H.; Valmu, L.; Nuutila, A.M.; Oksman-Caldentey, K.M.; Niklander-Teeri, V.
Source:
Transgenic research 2006 v.15 no.3 pp. 359-373
ISSN:
0962-8819
Subject:
recombinant DNA,   etc   ; Hordeum vulgare; barley; transgenic plants; gene expression; enterotoxigenic Escherichia coli; fimbriae; adhesins; recombinant antigens; promoter regions; transgenes; cytochemistry; endoplasmic reticulum; glycosylation; receptors; binding capacity; vaccines; vaccination; antibody formation; mice;     Show all 20 Subjects
DOI:
10.1007/s11248-006-0010-7
PubMed:
16779651

7. Plasmids for expression of heterologous proteins in Rhizopus oryzae

Author:
Mertens, J.A.; Skory, C.D.; Ibrahim, A.S.
Source:
Archives of microbiology 2006 v.186 no.1 pp. 41
ISSN:
1432-072X
Subject:
recombinant DNA,   etc   ; Rhizopus oryzae; genetically engineered microorganisms; genetic transformation; plasmid vectors; promoter regions; glucan 1,4-alpha-glucosidase; pyruvate decarboxylase; phosphoglycerate kinase; reporter genes; green fluorescent protein; gene expression; messenger RNA;     Show all 13 Subjects
Abstract:
... Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase ...
Handle:
10113/985
DOI:
10.1007/s00203-006-0121-9
PubMed:
16804680

8. Selective enhancement of the type 1 cytokine response by expression of a canine interleukin (IL)-12 fused heterodimeric DNA

Author:
Saldarriaga, O.A.; Perez, L.E.; Travi, B.L.; Melby, P.C.
Source:
Veterinary immunology and immunopathology 2006 v.110 no.3-4 pp. 377-388
ISSN:
0165-2427
Subject:
recombinant DNA,   etc   ; dogs; pets; interleukin-12; immune response; protein synthesis; gene expression; complementary DNA; cell-mediated immunity; vaccines; vaccination; messenger RNA; promoter regions; vaccine adjuvants;     Show all 14 Subjects
Abstract:
... Interleukin-12 (IL-12) is a heterodimeric cytokine that is a principal mediator of the innate immune response and modulator of acquired cell-mediated immunity. Administration of exogenous IL-12 can direct the host adaptive T cell response toward a type 1 phenotype. The co-administration of IL-12 with vaccine antigens has been shown to augment the vaccine-induced TH1 response and protection against ...
DOI:
10.1016/j.vetimm.2005.11.003

9. Transgenic Zebrafish Expressing Chicken Lysozyme Show Resistance against Bacterial Diseases

Author:
Yazawa, R.; Hirono, I.; Aoki, T.
Source:
Transgenic research 2006 v.15 no.3 pp. 385-391
ISSN:
0962-8819
Subject:
recombinant DNA,   etc   ; Danio rerio; transgenic animals; genetic transformation; injection; chickens; egg albumen; lysozyme; genes; promoter regions; keratin; Paralichthys olivaceus; gene expression; messenger RNA; disease resistance; Flavobacterium columnare; Edwardsiella tarda; bacterial infections; fish diseases; animal pathogenic bacteria;     Show all 20 Subjects
DOI:
10.1007/s11248-006-0009-0
PubMed:
16779653