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1. Expression of the 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Gene Requires Symbiotic Nitrogen-Fixing Regulator Gene nifA2 in Mesorhizobium loti MAFF303099

Author:
Nukui, Noriyuki; Minamisawa, Kiwamu; Ayabe, Shin-Ichi; Aoki, Toshio
Source:
Applied and environmental microbiology 2006 v.72 no.7 pp. 4964-4969
ISSN:
0099-2240
Subject:
recombinant DNA,   etc   ; Mesorhizobium loti; regulator genes; transcription factors; gene expression; transcription (genetics); amidine hydrolases; promoter regions; reporter genes; beta-glucuronidase; root nodules; nodulation; Lotus corniculatus var. japonicus;     Show all 13 Subjects
Abstract:
... Many soil bacteria contain 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which degrades ACC, a precursor of the phytohormone ethylene. In order to examine the regulation of the acdS gene encoding ACC deaminase in Mesorhizobium loti MAFF303099 during symbiosis with the host legume Lotus japonicus, we introduced the β-glucuronidase (GUS) gene into acdS so that GUS was expressed under contro ...
DOI:
10.1128/AEM.02745-05
PubMed:
16820494
PubMed Central:
PMC1489367

2. Plasmids for expression of heterologous proteins in Rhizopus oryzae

Author:
Mertens, J.A.; Skory, C.D.; Ibrahim, A.S.
Source:
Archives of microbiology 2006 v.186 no.1 pp. 41
ISSN:
1432-072X
Subject:
recombinant DNA,   etc   ; Rhizopus oryzae; genetically engineered microorganisms; genetic transformation; plasmid vectors; promoter regions; glucan 1,4-alpha-glucosidase; pyruvate decarboxylase; phosphoglycerate kinase; reporter genes; green fluorescent protein; gene expression; messenger RNA;     Show all 13 Subjects
Abstract:
... Rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. In this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae. Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase ...
Handle:
10113/985
DOI:
10.1007/s00203-006-0121-9
PubMed:
16804680