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... A procedure for protoplast isolation and plant regeneration of St. John's wort has been developed to utilize cell-to-cell variability for optimum production of valuable medicinal compounds. Calluses, induced from hypocotyl segments of St. John's wort seedlings, were used for protoplast isolation, induction of sustained cell division, and ultimately, plant regeneration. Callus-isolated protoplasts ...
Mucuna pruriens; root exudates; chemical constituents of plants; L-dopa; quantitative analysis; allelochemicals; bioassays; Lactuca sativa; lettuce; plant growth; growth retardation; cultivars; genetic variation; United States; Brazil; Taiwan; Thailand; Nepal
Abstract:
... We investigated whether or not lettuce growth was inhibited by diffused L-3-(3,4-dihydroxyphenyl)alanine (L-DOPA), an allelochemical exuded from the roots of velvetbean (Mucuna pruriens (L.) DC. var. utilis) cultivars using a modified plant-box bioassay. For all the cultivars and one accession examined L-DOPA diffused from the roots and caused radicle and hypocotyl growth inhibition. A high correl ...
Capsicum annuum; genetic variation; germplasm conservation; hot peppers; Guatemala
Abstract:
... The genetic diversity of 74 chili pepper accessions (Capsicum spp.) was evaluated with amplified fragment length polymorphisms (AFLPs). Thirty-four accessions were collected from home gardens throughout the Department of Alta Verapaz, Guatemala. The remaining accessions were selected from the national ex situ germplasm collection representing the diversity of 12 other departments of Guatemala. Mos ...
... Little information is available on the genetic diversity of hardinggrass (Phalaris aquatica L.), a cool-season forage grass with potential for use in the southern Great Plains. The objective of this study was to determine the genetic diversity within and among 22 promising hardinggrass populations, including plant introductions (PIs), breeding populations, and one cultivar. Nine plants from each p ...
... The genetic diversity of 74 chili pepper accessions (Capsicum spp.) was evaluated with amplified fragment length polymorphisms (AFLPs). Thirty-four accessions were collected from home gardens throughout the Department of Alta Verapaz, Guatemala. The remaining accessions were selected from the national ex situ germplasm collection representing the diversity of 12 other departments of Guatemala. Mos ...
Uniola paniculata; germplasm; genotype; genetic variation; amplified fragment length polymorphism; genetic markers; geographical variation; population structure; genetic polymorphism; Gulf of Mexico region; Southeastern United States
Abstract:
... Uniola paniculata, commonly known as sea oats, is a C4 perennial grass capable of stabilizing sand dunes. It is most abundant along the Gulf of Mexico and southeastern Atlantic coastal regions of the United States. The species exhibits low seed set and low rates of germination and seedling emergence, and so extensive clonal reproduction is achieved through production of rhizomes, which may contrib ...
... We propose a methodology combining a biophysical site index model and a seed source transfer model based on both temperature and precipitation to estimate white spruce plantation yield under present and future global warming conditions. The biophysical site index model predicts dominant height at 25 years, which is further used to estimate plantation yield using yield tables. The transfer model sh ...
... The island of Madeira harbours highly differentiated Robertsonian (Rb) races of the house mouse (Mus musculus domesticus), carrying several unique Rb fusions. The reproductive and geographical isolation between these races is expected to restrict gene flow severely, and contribute to their genetic differentiation. In the present study, the chromosomal distribution and frequency of transcriptionall ...
... An efficient Agrobacterium-mediated method for transformation of popular Bangladeshi Indica rice genotypes has been developed. Mature embryo-derived calluses as well as immature embryos were used as the target material. Transgenic plant production frequency was higher using the immature embryos than mature embryo-derived calluses. However, 3-week-old mature embryo-derived calluses served as an exc ...
... Cultivated timothy (Phleum pratense L.) is an important grass for hay production in temperate North America. It is underutilized in management-intensive rotational grazing systems because of its poor persistence when frequently defoliated. The objective of this study was to compare families selected for forage yield under frequent defoliation, families derived from old turf sods, and cultivars for ...
... The presence of the paralytic shellfish poisoning (PSP) dinoflagellate Alexandrium catenella in the north western (NW) Mediterranean Sea has been known since 1983. From this date on, the species has spread along the Spanish and Italian coastlines. Information concerning A. catenella isolates in the NW Mediterranean Sea was gained through phylogenetic studies. Twenty established toxic cultures of A ...
... A single nucleotide polymorphism (SNP; C vs. T) that creates an extra GATA-1 site (T allele) in intron 4 of the swine erythropoietin receptor (EPOR) gene was discovered and a genotyping assay for this SNP was developed. A total of 402 gilts from lines selected either at random (control), for ovulation rate (OR) or for uterine capacity (UC) for 11 generations were unilaterally hysterectomized-ovari ...
... Previous comparisons between the cDNA and gene sequences for secreted folate binding protein (sFBP) indicated a 12-bp insertion/deletion (ins/del) polymorphism in exon 1 and a SNP that altered (Ser-Arg) the protein AA sequence. The effect of the Ser-Arg SNP on reproductive traits was examined in three groups of Meishan-White European breed crossbred gilts. The gilts for all three groups were unila ...
... Adverse effects of gibberellin applications on pear trees after frost such as small fruit size, abnormal fruit shape and poor return bloom are often attributed both to the sole use of GA3 and its overdose. It is unclear whether protection against spring frosts before flower opening is more efficient when GA is applied directly after frost, i.e. before flower opening, or at full bloom or both. In A ...
... This study was conducted to quantify genetic variation within and between stocks of hatchery reared Siamese fighting fish, Betta splendens, which is an economically important ornamental fish in Thailand. Our preliminary study showed that a natural population of Siamese fighting fish had average number of alleles/locus of 1.5, 45.5% polymorphic loci and H(o) = 0.065. Fourteen stocks of Siamese figh ...
Luffa acutangula; alleles; allozymes; cultivars; fruits; genetic variation; loci; male flowers; seeds; small fruits; vegetables; China; India; Laos; Nepal
Abstract:
... The immature fruits of domesticated Luffa acutangula (L.) Roxb. are a common vegetable in Asia and India. To learn more about traditional cultivars, accessions were collected from southern Yunnan Province of China, northern Laos, and southeastern Nepal, and assessed for various parameters of genetic diversity. The size and shape of the fruits and seeds varied considerably. A form that we found cul ...
El-Lithy, Mohamed E.; Rodrigues, Gustavo C.; van Rensen, Jack J. S.; Snel, Jan F. H.; Dassen, Hans J. H. A.; Koornneef, Maarten; Jansen, Marcel A. K.; Aarts, Mark G. M.; Vreugdenhil, Dick
... Natural variation for photosynthetic traits was studied by determining chlorophyll fluorescence parameters in a collection of Arabidopsis accessions. This screen revealed only one single accession (Ely), exhibiting photosynthetic characteristics markedly different from all others, while a few lines showed small but significant variation. Detailed genetic and physiological analyses showed reduced f ...
... Aluminum (Al) distribution among several cellular fractions was investigated in root tips of seedlings of one Al-resistant and one Al-sensitive family of slash pine (Pinus elliottii Engelm.) and loblolly pine (Pinus taeda L.) grown in nutrient solution containing 100 μM AlCl3 (pH 4) for 167 h. Aluminum present in 5-mm-long root tips was fractionated into cell-wall-labile (desorbed in 0.5 mM citric ...
crops; Pythium irregulare; plant pathogenic fungi; pathogenicity; pathotypes; genetic variation; internal transcribed spacers; amplified fragment length polymorphism; diagnostic techniques; genetic markers; DNA fingerprinting; United States
Abstract:
... Pythium irregulare is a plant-pathogenic oomycete that causes significant damage to a variety of crops, including ornamentals and vegetables. Morphological as well as molecular studies have reported high levels of genetic diversity within P. irregulare sensu lato which has raised the question as to whether it is a single species or is actually a complex of morphologically similar (cryptic) species ...
... Acid-PAGE analysis of gliadins from 159 Indian wheat (Triticum aestivum L.) cultivars developed during the last five decades was accomplished to identify gliadin band patterns as well as the extent of genetic diversity. Extensive polymorphism [genetic diversity index (H) = 0.875] in gliadin pattern was observed in the cultivars studied. A total of 147 band patterns were identified, of which 45 dif ...
... Acid-PAGE analysis of gliadins from 159 Indian wheat (Triticum aestivum L.) cultivars developed during the last five decades was accomplished to identify gliadin band patterns as well as the extent of genetic diversity. Extensive polymorphism [genetic diversity index (H) = 0.875] in gliadin pattern was observed in the cultivars studied. A total of 147 band patterns were identified, of which 45 dif ...
Citrullus; random amplified polymorphic DNA technique; microsatellite repeats; watermelons; plant breeding; genetic improvement; cucumbers; genetic distance; phylogeny; plant genetic resources; Cucumis; disease resistance; genetic variation; pest resistance
Abstract:
... A cucurbit species named Praecitrullus fistulosus (Stocks) Pangalo, which thrives in India, is considered to be a distant relative of watermelon. Recent experiments indicated that it has mild resistance to whiteflies (Bemisia tabaci). However, our attempts to cross various US plant introductions (PIs) of P. fistulosus with watermelon or other Citrullus PIs have not been successful. Thus, to determ ...
canola; races; alleles; Plenodomus lingam; fungal diseases of plants; pathotypes; geographical variation; pathogenicity; plant pathogenic fungi; disease resistance; genetic variation; Brassica napus var. napus; Canada; Australia; Europe
Abstract:
... Leptosphaeria maculans, the causal agent of stem canker of oilseed rape, develops gene-for-gene interactions with its hosts. To date, eight L. maculans avirulence (Avr) genes, AvrLm1 to AvrLm8, have been genetically characterized. An additional Avr gene, AvrLm9, that interacts with the resistance gene Rlm9, was genetically characterized here following in vitro crosses of the pathogen. A worldwide ...
... The aggressiveness of an extensive collection of strains of Erwinia amylovora was analyzed using immature fruit and detached pear flower assays under controlled environmental conditions. The analysis was performed by means of a quantitative approach based on fitting data to mathematical models that relate infection incidence to pathogen dose and time. Probit and hyperbolic saturation models were u ...
restriction fragment length polymorphism; geographical distribution; Cochliomyia hominivorax; haplotypes; mitochondrial DNA; cytochrome-c oxidase; genetic variation; population structure; Uruguay
Abstract:
... The New Word screwworm, Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is one of the most important insect pests of livestock in the Neotropical region. In this work, polymerase chain reaction-restriction fragment length polymorphism of mitochondrial DNA (mtDNA) was used to study the diversity and population structure of seven geographically distinct populations of C. hominivora ...
... Crinipellis perniciosa causes a serious disease of cacao known as witches' broom (WB). Heritable resistance to witches' broom has been used in cacao improvement programs. 'SCA6' and 'SCA12' are highly resistant and are the most commonly used parents in the breeding schemes. However, SCA hybrids are not resistant to witches' broom in all production areas. Presumably, different populations of C. per ...
... A selection of evaluations from Faculty of 1000 Biology: yeast metabolite profiling; high-frequency homologous recombination; functional RNA motifs; phylogeny of Oryzeae; genomic variability within an organism. ...
fish farms; Acipenser; sturgeon; freshwater fish; genetic variation; animal reproduction; spawning; hermaphroditism; fish culture; freshwater aquaculture
Abstract:
... Hormonal stimulation of sterlet sturgeon, Acipenser ruthenus, was carried out with carp pituitary extract at 2 and 5 mg kg(-1) body weight for males on the one hand, females and hermaphrodites on the other hand. Within our experimental stock, reproductive performances defined by relative number of ovulated females, fertilisation rate, and relative number of good quality semen samples had begun to ...
Cyprinus carpio; carp; freshwater fish; females; genetic variation; spawning; pituitary gland; ovulation; body weight; fish eggs; egg quality; fish culture; freshwater aquaculture; Poland; Hungary
Abstract:
... Investigations of the effects of controlled reproduction were conducted on carp females of Polish strain 6 and Hungarian strain W after ovulation stimulation with carp pituitary homogenate (CPH) (0.3 mg+2.7 mg kg(-1) body weight), Ovopel (1/5+1 pellet kg(-1) body weight) or Dagin (1 dose kg(-1) body weight). In both strains, the highest percentage of spawning females was found after the Ovopel tre ...
temporal variation; geographical distribution; microsatellite repeats; migratory behavior; Helicoverpa armigera; gene flow; genetic variation; population structure; Australia
Abstract:
... Analysis of gene flow and migration of Helicoverpa armigera (Hubner) in a major cropping region of Australia identified substantial genetic structuring, migration events, and significant population genotype changes over the 38-mo sample period from November 1999 to January 2003. Five highly variable microsatellite markers were used to analyze 916 individuals from 77 collections across 10 localitie ...
... To quantify genetic diversity among modern and earlier maize cultivars, 133 varieties, representative of the maize grown in France during the last five decades, were fingerprinted using 51 SSR. The varieties were grouped into four periods. For each period, allelic richness, genetic diversity and genetic differentiation among periods were computed. A total of 239 alleles were generated. Allelic ric ...
... Ten highly variable microsatellite loci were used to investigate one common population and two selected hatchery populations of Japanese flounder Paralichthys olivaceus. All of the 10 microsatellite loci screened in this study showed marked polymorphism. A total of 76 different alleles were observed over all loci. The number of alleles per locus ranged from 3.67 to 8.67. The average of observed (H ...
... Three red rice ecotypes (Oryza spp), including LA 5, MS 5 and TX 4, were evaluated for acetolactate synthase resistance/tolerance to imazethapyr. The red rice ecotypes were compared with a tolerant line (CL-121), a resistant line (CL-161) and a conventional rice variety (Cypress). Based on enzymatic activity, the mean I50 values were 1.5, 1.1, 1.5, 1.6, 20.8 and 590.6 micromolar imazethapyr, respe ...
Tectona grandis; forest trees; genetic variation; genetic markers; amplified fragment length polymorphism; plant genetic resources; population genetics; India; Thailand; Indonesia
Abstract:
... There is much concern over teak (Tectona grandis L. f.) genetic resources because of uncontrolled logging during the past centuries. We investigated patterns of genetic variation within and among nine populations of T. grandis from diverse geographical regions in India, Thailand, and Indonesia using amplified fragment length polymorphism (AFLP). An analysis of molecular variance (AMOVA) showed tha ...
... Efficient assessment of genetic diversity is important for conservation and utilization of genetic resources. We sought to assess the ability of inter simple sequence repeats (ISSRs) as molecular markers to identify genetic diversity both within and among sympatric populations of common bean, Phaseolus vulgaris L. One wild and four domesticated populations originating from the Sierra Norte de Pueb ...
... Twenty-one Citrus tristeza virus (CTV) isolates from India were characterized, using genotype-specific multiple molecular markers (MMM) from the 54′-terminal region and two other overlapping primer pairs (CN487/489 and CN488/491) from ORF1a (697–1484 nucleotides (nt)). The 5′-terminal genotype-specific primer pairs amplified about 500 bases from the 5′-end of the CTV genomic RNA (gRNA). With the t ...