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- Li, S.Y.
- Journal of economic entomology 2005 v.98 no.6 pp. 1870-1875
- insect viruses; dose response; Baculoviridae; biological control agents; Neodiprion; sawflies; instars; larvae; sublethal effects; virulence; mortality
- ... Different larval stages of balsam fir sawfly, Neodiprion abietis (Harris) (Hymenoptera: Diprionidae), were challenged by different concentrations of a nucleopolyhedrovirus in the laboratory to determine larval susceptibility to the virus and to test treatment effects of the virus on the sawfly survivors. The results indicated that younger larvae were more susceptible to the virus than older instar ...
- Rincon-Castro, M.C. del; Ibarra, J.E.
- Biocontrol science and technology 2005 v.15 no.7 pp. 701-710
- viral insecticides; recombinant DNA; Trichoplusia ni; Baculoviridae; biological control agents; strain differences; virulence; Autographa californica; genes; larvae; microbial genetics; mortality; biological control
- ... A recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) strain showing higher virulence against Trichoplusia ni larvae than the wild-type virus was developed. The 'enhancin' (VEF) gene of T. ni granulovirus (TnGV) and the AcMNPV polyhedrin gene were cloned into the baculovirus transfer vector pAcUW31. This plasmid and AcMNPV BacPAK6 DNA were co-transfected into the BTI-Tn5B1-4 cell line ...
- Barreto, M.R.; Guimaraes, C.T.; Teixeira, F.F.; Paiva, E.; Valicente, F.H.
- Neotropical entomology 2005 v.34 no.1 pp. 67-75
- Baculoviridae; biological control agents; genetic distance; genetic polymorphism; genetic variation; biological control; insect control; Spodoptera frugiperda; moths; insect pests; plant pests; larvae; mortality; larval development; pupal development; duration; lethal dose 50; random amplified polymorphic DNA technique
- ... The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th an ...