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- Pupillo, P., et al. Show all 4 Authors
- Planta 2005 v.220 no.3 pp. 365-375
- polypeptides; beans; plant biochemistry; hypocotyls; Phaseolus vulgaris; microsomes; membrane proteins; cytochrome b; tonoplast; ascorbic acid
- ... Two membrane-bound, ascorbate-dependent b-type cytochromes were identified in etiolated bean (Phaseolus vulgaris L.) hypocotyls. Following solubilization of microsomal membranes and anion-exchange chromatography at pH 8.0, two major cytochrome peaks (P-I and P-II) were separated. Both cytochromes were reduced by ascorbate and re-oxidized by monodehydroascorbate, but P-I reduction by ascorbate was ...
- Pupillo, P., et al. Show all 6 Authors
- Plant physiology 2005 v.138 no.4 pp. 2210-2219
- photosynthesis; glyceraldehyde-3-phosphate dehydrogenase; isozymes; redox reactions; protein quaternary structure; plant biochemistry; plant genetics; amino acid sequences; nucleotide sequences
- ... Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of higher plants catalyzes an NADPH-consuming reaction, which is part of the Calvin cycle. This reaction is regulated by light via thioredoxins and metabolites, while a minor NADH-dependent activity is constant and constitutive. The major native isozyme is formed by A- and B-subunits in stoichiometric ratio (A2B2, A8B8), but tetramers of recombinant ...
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