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You searched for: Publication year rev 7998-2002 Remove constraint Publication year rev: 7998-2002 Subject term Western blotting Remove constraint Subject term: Western blotting
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1. Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64

Author:
Alexandra Spenger; Reingard Grabherr; Lars Töllner; Hermann Katinger; Wolfgang Ernst
Source:
European journal of biochemistry 2002 v.269 no.18 pp. 4458-4467
ISSN:
0014-2956
Subject:
Autographa californica; Autographa californica multiple nucleopolyhedrovirus; Human immunodeficiency virus 1; Western blotting; antigens; binding properties; enzyme-linked immunosorbent assay; genetic engineering; glycoproteins; host range; humans; insects; insertional mutagenesis; membrane fusion; molecular biology; mutants; peptides; probability; receptors; viral envelope proteins; viruses
Abstract:
... The envelope protein gp64 of the baculovirus Autographa californica nuclear polyhedrosis virus is essential for viral entry into insect cells, as the glycoprotein both mediates pH‐dependent membrane fusion and binds to host cell receptors. Surface modification of baculovirus particles by genetic engineering of gp64 has been demonstrated by various strategies and thus has become an important and ...
DOI:
10.1046/j.1432-1033.2002.03135.x

2. An antibody to recombinant crustacean hyperglycaemic hormone of Nephrops norvegicus cross-reacts with neuroendocrine organs of several taxa of malacostracan Crustacea

Author:
P. G. Giulianini; N. Pandolfelli; S. Lorenzon; E. A. Ferrero; P. Edomi
Source:
Cell and tissue research 2002 v.307 no.2 pp. 243-254
ISSN:
0302-766X
Subject:
Astacus leptodactylus; Nephrops norvegicus; Palaemon; Squilla mantis; Western blotting; antiserum; bioassays; complementary DNA; cross reaction; glutathione transferase; hormones; immunocytochemistry; monitoring; nerve endings; neurons; neuropeptides; phylogeny; physiology; precipitin tests; rabbits; shrimp; transmission electron microscopy
Abstract:
... The crustacean hyperglycaemic hormones (cHHs) are multifunctional neuropeptides that play a central role in the physiology of crustaceans. A partial cDNA coding for cHH of the Norway lobster, Nephrops norvegicus, was cloned; this cDNA was fused to glutathione-S-transferase (GST) to obtain a recombinant fusion protein that was used to raise a rabbit antiserum and to perform a biological assay. The ...
DOI:
10.1007/s00441-001-0484-9
PubMed:
11845331

3. An immunochemical technique for the detection of ovalbumin in surimi-derived products

Author:
Romero-Rodriguez, Ma Angeles; Barcia-Vieitez, Ramiro; Vazquez-Oderiz, Ma Lourdes
Source:
Journal of the science of food and agriculture 2002 v.82 no.14 pp. 1614-1616
ISSN:
0022-5142
Subject:
Western blotting; actin; detection limit; egg albumen; fish; muscle protein; ovalbumin; surimi
Abstract:
... Egg white is frequently used in surimi-derived products. However, electrophoretic detection of egg white in such products is not reliable, since the egg white protein ovalbumin and the fish muscle protein actin have very similar molecular weights. We report the development of an immunoblotting (Western blotting) technique for the detection of ovalbumin in products of this type. We initially used a ...
DOI:
10.1002/jsfa.1213

4. Assessment of the floral origin of honey by SDS-page immunoblot techniques

Author:
Baroni, M.V.; Chiabrando, G.A.; Costa, C.; Wunderlin, D.A.
Source:
Journal of agricultural and food chemistry 2002 v.50 no.6 pp. 1362-1367
ISSN:
0021-8561
Subject:
cross reaction; Helianthus annuus; Eucalyptus; biomarkers; polyacrylamide gel electrophoresis; Western blotting; pollen; honey
Abstract:
... We report on the development of a novel alternative method for the assessment of floral origin in honey samples based on the study of honey proteins using immunoblot assays. The main goal of our work was to evaluate the use of honey proteins as chemical markers of the floral origin of honey. Considering that honeybee proteins should be common to all types of honey, we decided to verify the usefuln ...
DOI:
10.1021/jf011214i
PubMed:
11879003

5. Brassica napus soluble epoxide hydrolase (BNSEH1): Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris

Author:
Stefan Bellevik; Jiaming Zhang; Johan Meijer
Source:
European journal of biochemistry 2002 v.269 no.21 pp. 5295-5302
ISSN:
0014-2956
Subject:
fatty acids; Komagataella pastoris; polyacrylamide gel electrophoresis; Western blotting; gel chromatography; Brassica napus; antibodies; recombinant proteins; rapid amplification of cDNA ends; amino acids; molecular weight; screening; leaves; Arabidopsis thaliana; complementary DNA; yeasts; seed oils; methyl jasmonate; epoxide hydrolase; cDNA libraries; fatty acid metabolism; hydrolysis; temperature; metabolic engineering; epoxides; pH
Abstract:
... Epoxide hydrolase (EC 3.3.2.3) in plants is involved in the metabolism of epoxy fatty acids and in mediating defence responses. We report the cloning of a full‐length epoxide hydrolase cDNA (BNSEH1) from oilseed rape (Brassica napus) obtained by screening of a cDNA library prepared from methyl jasmonate induced leaf tissue, and the 5′‐RACE technique. The cDNA encodes a soluble protein contai ...
DOI:
10.1046/j.1432-1033.2002.03247.x

6. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

Author:
A. J. Fales-Williams; K. A. Brogden; E. Huffman; J. M. Gallup; M. R. Ackermann
Source:
Veterinary pathology v.39 no.6 pp. 706-711
ISSN:
1544-2217
Subject:
Mannheimia; Pasteurella; Pseudomonas aeruginosa; Western blotting; adults; animal pathology; antibodies; antimicrobial peptides; bacteria; bronchioles; calves; cows; humans; immunohistochemistry; inflammation; inoculum; lipopolysaccharides; respiratory tract diseases; sheep
Abstract:
... Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with Mannheimia (Paste ...
DOI:
10.1354/vp.39-6-706

7. Characterisation of antisera to recombinant IgA of the common brushtail possum (Trichosurus vulpecula)

Author:
Rawson, Renee R.; Belov, Katherine; Gidley-Baird, A. Angus; Cooper, Desmond W.
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 89-95
ISSN:
0165-2427
Subject:
Macropus eugenii; Macropus giganteus; Phascolarctos cinereus; Trichosurus vulpecula; Western blotting; antiserum; blood serum; cross reaction; enzyme-linked immunosorbent assay; immunoglobulin A; immunoglobulin G; mucosal immunity; possums; recombinant proteins
Abstract:
... One of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. This paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of IgA (Cα) of the common brushtail possum (Trichosurus vulpecula). The availability of a marsupial specific anti-IgA provide ...
DOI:
10.1016/S0165-2427(02)00126-5

8. Characterisation of the first Australian isolate of Neospora caninum from cattle

Author:
Miller, C.M.D.; Quinn, H.E.; Windsor, P.A.; Ellis, J.T.
Source:
Australian veterinary journal 2002 v.80 no.10 pp. 620-625
ISSN:
0005-0423
Subject:
Holstein; Neospora caninum; Toxoplasma gondii; Western blotting; antigens; calves; cerebrum; coasts; containers; dairy herds; electron microscopy; encephalitis; histopathology; mice; nucleotide sequences; parasites; pregnancy; pups; sequence analysis; serology; seroprevalence; spinal cord; tissue culture; transplacental transmission; vaccine development; virulence; Australia
Abstract:
... Objective To isolate Neospora caninum from a congenitally infected calf. Procedure A calf was obtained from a N caninum infected dam maintained in a dairy herd of Holstein-Friesian cattle located on the south coast of NSW near Nowra. The calf was euthanased and samples collected for serology and pathology. Samples of brain and spinal cord of the calf were homogenised and injected into immunocompro ...
DOI:
10.1111/j.1751-0813.2002.tb10967.x

9. Characterization of the lectin from females of Phlebotomus duboscqi sand flies

Author:
Petr Volf; Sona Skarupová; Petr Man
Source:
European journal of biochemistry 2002 v.269 no.24 pp. 6294-6301
ISSN:
0014-2956
Subject:
Leishmania; Lutzomyia longipalpis; Phlebotomus duboscqi; Phlebotomus papatasi; Western blotting; antibodies; cDNA libraries; carbohydrate binding; complementary DNA; epithelial cells; females; fluorescent antibody technique; gel chromatography; hemagglutination; immunoaffinity chromatography; immunoglobulins; lectins; matrix-assisted laser desorption-ionization mass spectrometry; midgut; molecular weight; polyacrylamide gel electrophoresis; salivary glands; silver nitrate
Abstract:
... Lectin from females of the important sand fly vector, Phlebotomus duboscqi (Diptera: Psychodidae), was isolated by immunoaffinity chromatography using a minicolumn with immobilized anti‐lectin immunoglobulins. Carbohydrate‐binding specificity of active fractions corresponded to that of midgut and salivary gland lysates. Haemagglutination was inhibited by d‐glucosamine, d‐galactosamine and d‐mannos ...
DOI:
10.1046/j.1432-1033.2002.03349.x

10. Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity

Author:
Liu, M.; Cai, Q.X.; Liu, H.Z.; Zhang, B.H.; Yan, J.P.; Yuan, Z.M.
Source:
Journal of applied microbiology 2002 v.93 no.3 pp. 374-379
ISSN:
1364-5072
Subject:
Bacillus thuringiensis; Spodoptera exigua; Western blotting; bioassays; biopesticides; chitinase; colorimetry; insecticidal properties; larvae; molecular weight; pH; polyacrylamide gel electrophoresis; serotypes; synergism; toxicity
Abstract:
... Aims: To investigate the distribution of chitinase in Bacillus thuringiensis strains, and the enhancing effects of the chitinase-producing B. thuringiensis strains on insecticidal toxicity of active B. thuringiensis strain against Spodoptera exigua larvae. Methods and Results: The chitinolytic activities of B. thuringiensis strains representing the 70 serotypes were investigated by the whitish opa ...
DOI:
10.1046/j.1365-2672.2002.01693.x
PubMed:
12174034

11. Cloning, expression, purification, and biological activity of five feline type I interferons

Author:
Wonderling, Ramani; Powell, Tim; Baldwin, Susan; Morales,Tony; Snyder, Scott; Keiser, Kathy; Hunter, Shirley; Best, Elaine; McDermott, Martin J.; Milhausen, Michael
Source:
Veterinary immunology and immunopathology 2002 v.89 no.1-2 pp. 13-27
ISSN:
0165-2427
Subject:
sequence analysis; interferon-alpha; antiviral properties; Komagataella pastoris; complementary DNA; cats; cell culture; glycosylation; humans; recombinant proteins; yeasts; Western blotting; amino acid sequences; amino acids; clones; genes; viruses; immune response
Abstract:
... Type I interferons (IFN) are important mediators of the host defense against viral infections in mammals. In humans multiple subtypes of IFN-α exist, most of which possess antiviral activity. Little is known about the type I IFN genes in cats and the role they may play in feline immunological responses to viruses. We have isolated cDNAs encoding five feline IFN-α (feIFN) subtypes that share from 9 ...
DOI:
10.1016/S0165-2427(02)00188-5

12. Comparison of a Putative Second Serotype of Chicken Infectious Anemia Virus with a Prototypical Isolate II. Antigenic and Physicochemical Characteristics

Author:
Spackman, E.; Cloud, S.S.; Rosenberger, J.K.
Source:
Avian diseases 2002 v.46 no.4 pp. 956-963
ISSN:
0005-2086
Subject:
Chicken anemia virus; DNA primers; Southern blotting; Western blotting; bird diseases; broiler breeders; chloroform; enzyme-linked immunosorbent assay; fluorescent antibody technique; neutralization tests; nucleotide sequences; pH; polymerase chain reaction; pullets; serotypes; thermal stability; viruses
Abstract:
... A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated included thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenica ...
DOI:
10.1637/0005-2086(2002)046[0956:COAPSS]2.0.CO;2

13. Concerted regulation of free arachidonic acid and hormone‐induced steroid synthesis by acyl‐CoA thioesterases and acyl‐CoA synthetases in adrenal cells

Author:
Paula Maloberti; Rocío Castilla Lozano; Pablo G. Mele; Florencia Cano; Cecilia Colonna; Carlos F. Mendez; Cristina Paz; Ernesto J. Podestá
Source:
European journal of biochemistry 2002 v.269 no.22 pp. 5599-5607
ISSN:
0014-2956
Subject:
Western blotting; acyl coenzyme A; antibodies; arachidonic acid; corticotropin; immunocytochemistry; immunohistochemistry; inhibitory concentration 50; long-chain-fatty-acid-CoA ligase; neoplasm cells; nordihydroguaiaretic acid; steroidogenesis; synergism
Abstract:
... Although the role of arachidonic acid (AA) in the regulation of steroidogenesis is well documented, the mechanism for AA release is not clear. Therefore, the aim of this study was to characterize the role of an acyl‐CoA thioesterase (ARTISt) and an acyl‐CoA synthetase as members of an alternative pathway in the regulation of the intracellular levels of AA in steroidogenesis. Purified recombina ...
DOI:
10.1046/j.1432-1033.2002.03267.x

14. Construction and Immunogenicity Studies of Recombinant Fowl Poxvirus Containing the S1 Gene of Massachusetts 41 Strain of Infectious Bronchitis Virus

Author:
Wang, Xiuqing; Schnitzlein, William M.; Tripathy, Deoki N.; Girshick, Theodore; Khan, Mazhar I.
Source:
Avian diseases 2002 v.46 no.4 pp. 831-838
ISSN:
0005-2086
Subject:
Infectious bronchitis virus; Western blotting; blood serum; chickens; complementary DNA; fluorescent antibody technique; fowl pox; genes; glycoproteins; humoral immunity; immune response; immunization; immunoglobulin G; infectious bronchitis; neutralization; neutralizing antibodies; staining; vaccines; virulence; virulent strains; viruses; Massachusetts
Abstract:
... The spike 1 (S1) surface glycoprotein of infectious bronchitis virus (IBV) is the major inducer of the generation of virus neutralizing antibodies, and the administration of purified S1 has been shown to elicit a protective immune response against virulent virus challenge. On the basis of these observations, recombinant fowl poxvirus (rFPV) containing a cDNA copy of the S1 gene of IBV Mass 41 (rFP ...
DOI:
10.1637/0005-2086(2002)046[0831:CAISOR]2.0.CO;2

15. Cross-sectional study of serum antibodies against Sarcocystis neurona in cats tested for antibodies against Toxoplasma gondii

Author:
Rossano, M.G.; Murphy, A.J.; Vrable, R.A.; Vanzo, N.E.; Lewis, S.K.; Sheline, K.D.; Kaneene, J.B.; Mansfield, L.S.
Source:
Journal of the American Veterinary Medical Association 2002 v.221 no.4 pp. 511-514
ISSN:
0003-1488
Subject:
cats; Sarcocystis; seroprevalence; antibodies; fluorescent antibody technique; Toxoplasma gondii; Western blotting; Michigan
DOI:
10.2460/javma.2002.221.511
PubMed:
12184700

16. Detection of antibodies against Sarcocystis neurona in cerebrospinal fluid from clinically normal neonatal foals

Author:
Cook, A.G.; Maxwell, V.B.; Donaldson, L.L.; Parker, N.A.; Ward, D.L.; Morrow, J.K.
Source:
Journal of the American Veterinary Medical Association 2002 v.220 no.2 pp. 208-211
ISSN:
0003-1488
Subject:
foals; Sarcocystis; cerebrospinal fluid; blood serum; antibody detection; protozoal infections; equine protozoal myeloencephalitis; Western blotting
DOI:
10.2460/javma.2002.220.208
PubMed:
12126132

17. Detection of ligands for selectins in the oligosaccharide fraction of human milk

Author:
Silvia Rudloff; Christina Stefan; Gottfried Pohlentz; Clemens Kunz
Source:
European journal of nutrition 2002 v.41 no.2 pp. 85-92
ISSN:
1436-6207
Subject:
Western blotting; breast milk; cell adhesion; epitopes; ethanolamine; immunoassays; milk; monoclonal antibodies; oligosaccharides; spectroscopy; thin layer chromatography
Abstract:
... Background Human milk contains a large variety of oligosaccharides which show structural similarities with ligands for selectins, a family of cell adhesion molecules which are involved in many cell-cell interactions. Aim of the study Due to their structural similarity with selectin ligands, human milk oligosaccharides were labelled with phosphatidyl ethanolamine to be able to investigate specific ...
DOI:
10.1007/s003940200012
PubMed:
12083318

18. Detection of rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers

Author:
Chomchan, P.; Miranda, G.J.; Shirako, Y.
Source:
Archives of virology 2002 v.147 no.12 pp. 2291-2300
ISSN:
0304-8608
Subject:
plant tissues; rice; insect pests; disease vectors; Nilaparvata lugens; plant diseases and disorders; cell walls; Oryza sativa; Rice grassy stunt virus; host plants; Western blotting; cytoplasm; nucleocapsid proteins; detection; leaves; homogenization; Philippines
DOI:
10.1007/s00705-002-0893-4
PubMed:
12491098

19. Development and characterization of monoclonal antibodies to chicken interleukin-15

Author:
Min, Wongi; Lillehoj, Hyun S.; Li, Guangxing; Sohn, Eun J.; Miyamoto, Tadashi
Source:
Veterinary immunology and immunopathology 2002 v.88 no.1-2 pp. 49-56
ISSN:
0165-2427
Subject:
T-lymphocytes; Western blotting; cell growth; chickens; enzyme-linked immunosorbent assay; genes; interleukin-15; interleukin-2; monoclonal antibodies; polypeptides
Abstract:
... The chicken IL-15 gene was recently cloned and shown to encode a polypeptide with T cell growth factor activity similar to IL-2. To further characterize the chemical and biological properties of chicken IL-15, we generated a panel of monoclonal antibodies against bacterially expressed protein and characterized their binding specificities. All antibodies were reactive by ELISA with recombinant IL-1 ...
DOI:
10.1016/S0165-2427(02)00123-X

20. Distribution of various peptides in citrus fruits (grapefruit, lemon, and orange)

Author:
Sass-Kiss, A.; Sass, M.
Source:
Journal of agricultural and food chemistry 2002 v.50 no.7 pp. 2117-2120
ISSN:
0021-8561
Subject:
interspecific variation; lemon juice; polyacrylamide gel electrophoresis; peptides; orange juice; adulterated products; Western blotting; grapefruit juice; detection
Abstract:
... Tissue- and species-specific peptides of the grapefruit have been investigated by SDS-PAGE and Western blot. Five peptides from the juice and one peptide from the peel were isolated by preparative gel electrophoresis. Polyclonal antibodies were developed against them in mice. It can be established that 82, 63, and 46 kDa peptides occurred exclusively in the samples prepared from the grapefruit and ...
DOI:
10.1021/jf0111615
PubMed:
11902965