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- Nishida, Tamotsu; Tanaka, Hirofumi; Yasuda, Hideyo
- European journal of biochemistry 2000 v.267 no.21 pp. 6423-6427
- histidine, etc ; Saccharomyces cerevisiae; active sites; cell nucleolus; cysteine; enzymes; humans; interphase; two hybrid system techniques; ubiquitin; yeasts; Show all 11 Subjects
- ... A novel Smt3âspecific isopeptidase, SMT3IP1, was cloned using a yeast twoâhybrid screen with Smt3b as bait. The clone, named SMT3IP1 (Smt3âspecific isopeptidase 1), which bound to Smt3b but not SUMOâ1 in the twoâhybrid system, was distantly related to budding yeast Saccharomyces cerevisiae Ulp1, human SENP1 or human SUSP1. The catalytic domains in the Câterminal region were very simila ...
- Sperling, Petra; Lee, Michael; Girke, Thomas; ZÃ¤hringer, Ulrich; Stymne, Sten; Heinz, Ernst
- European journal of biochemistry 2000 v.267 no.12 pp. 3801-3811
- histidine, etc ; Physcomitrella patens; Saccharomyces cerevisiae; clones; complementary DNA; enzymes; epoxides; fatty acids; genes; genetically modified organisms; mosses and liverworts; polymerase chain reaction; proteins; yeasts; Show all 14 Subjects
- ... Many plant genes have been cloned that encode regioselective desaturases catalyzing the formation of cisâunsaturated fatty acids. However, very few genes have been cloned that encode enzymes catalyzing the formation of the functional groups found in unusual fatty acids (e.g. hydroxy, epoxy or acetylenic fatty acids). Here, we describe the characterization of an acetylenase from the moss Ceratodo ...
- Ohta, D.; Fujimori, K.; Mizutani, M.; Nakayama, Y.; Kunpaisal-Hashimoto, R.; Munzer, S.; Kozaki, A.
- Plant physiology 2000 v.122 no.3 pp. 907-914
- histidine, etc ; Arabidopsis thaliana; pentosyltransferases; biochemical pathways; isozymes; chemical structure; complementary DNA; nucleotide sequences; amino acid sequences; messenger RNA; plant development; protein synthesis; leaves; mutants; Saccharomyces cerevisiae; enzyme inhibitors; enzyme activity; genetic complementation; genetic recombination; Show all 19 Subjects
- ... We have characterized two isoforms of ATP-phosphoribosyl transferase (ATP-PRT) from Arabidopsis (AtATP-PRT1 [accession no. AB025251] and AtATP-PRT2), catalyzing the first step of the pathway of hisidine (His) biosynthesis. The primary structures deduced from AtATP-PRT1 and AtATP-PRT2 cDNAs share an overall amino acid identity of 74.6% and contain N-terminal chloroplast transit peptide sequences. D ...
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